This study was conducted to analyze the productivity and economic feasibility of truss-limited tomatoes (Lycopersicon esculentum L.) grown at a high density in Cambodia during the rainy season. During the rainy season in Cambodia, due to excessive rain and very high temperature, tomatoes cannot grow well in an open field, leading to a greatly reduced yield. High density truss-limited cultivation (HDTLC) of tomatoes can shorten the growing season and increase yields. In conventional cultivation (control), tomatoes were transplanted at a spacing of 70×40 cm and harvested up to the 4th fruit cluster. In HDTLC, tomatoes were transplanted at a spacing of 20×20 cm or 25×25 cm and harvested up to the 2nd fruit cluster. Plant heights in HDTLC were higher than those in the control at 22 days after transplanting (DAT). At 39 DAT, plant heights did not differ between 25×25 cm and control. At 60 DAT, plant height, stem diameter, leaf length, and leaf width of the HDTLC were lower than those of the control. Fruit length and width were also lower in HDTLC compared to those of the control. However, sugar content was more than 19% higher in HDTLC compared to that in the control. Yield per plant of the control was the highest, but yields per ha in HDTLCs were 99% (20×20 cm) and 38% (25×25 cm) higher, respectively, compared to that of the control. No pesticides were used for disease control in this experiment. However, to ensure yield, it is recommended to observe the disease status of plants and spray pesticides at the appropriate time. The disease index (1: disease-free; 5: all leaves were disease-infected) of late blight (Phytophthora indestans) was significantly higher for 20×20 cm (4.5) and 25×25 cm (3.6) groups compared to the control (2.0). Income with 20×20 cm (5,668 thousand KRW) and 25×25 cm (1,863 thousand KRW) were 4.9 and 1.6 times higher, respectively, compared to that with the control (1,149 thousand KRW). So, we want to spread the HDTLC technology, which transplanting of tomato seedlings at 20×20 cm intervals and harvests up to 2nd fruit cluster, to Cambodian farmers.
본 실험은 위점막에 존재하는 H. pylori에 대한 PJT 추출물의 생장 억제를 알아보고자 하였다. H. pylori에 대한 치료제로는 항생제가 있으나 높은 내성률 등의 문제가 야기되 고 있다. 천연물은 화합물보다 높은 안전성과 항생제 내성 문제의 해결 측면에서 큰 장점을 가지고 있어 예방 및 치료제의 개발로 많은 연구가 진행되고 있다. PJT는 활성화와 항알레르기 작용 및 실험성 위궤양의 항궤양 작용에 우수한 것으로 알려져 있다. 또한 뇌 졸중으로 발병되는 중풍병, 해독 등의 효능이 있어 활용성이 높은 약초로 향후 연구대상 약용작물로 각광을 받을 가능성이 큰 항균성 약용작물이다.
실험에서 사용된 H. pylori 균은 멸균된 플레이트에 접종한 후, 10% CO2 및 100% 습 도가 유지되는 37℃ incubation에 3일간 배양 후 평가하였다. 균이 접종된 플레이트에 각각 표준자를 이용하여 6시간, 12시간, 24시간 및 48시간 후 clear zone의 지름으로 표현되는 억제범위(the zone of inhibition)를 측정하여 균이 자라지 않은 clear zone을 관찰하였다.
실험 종료 후, H. pylori 생장 억제 활성 측정결과 에탄올, 열수 추출물에서의 생장 억제를 확인할 수 있었다. 본 실험에서 열수 추출보다 에탄올 추출에서 더 나은 저해 활성을 나타난 것을 확인할 수 있었고, 이것은 물과 에탄올 즉 용매의 극성이 각각 다르기 때문에 차이가 나는 것으로 사료된다. 따라서 PJT은 H. pylori를 효과적으로 억제하는 것으로 판단되며, 향후 H. pylori에 대한 갯기름나물 항궤양 효과에 지표로 쓰일 수 있을 것으로 판단된다.
In this project, ECP(Electrical conducting polymer) gasket is used to detect the leakage of chemicals inside the reservoir. It is necessary that the electrical signal between ECP gasket and chemical leakage should be detected to protect the critical accident. This research introduce the leakage detection method to measure the electrical resistance of the cracked prototype pipe installed ECP gasket. Also the Arduino program is set to monitor the incident of chemical leakage.