In this project, ECP(Electrical conducting polymer) gasket is used to detect the leakage of chemicals inside the reservoir. It is necessary that the electrical signal between ECP gasket and chemical leakage should be detected to protect the critical accident. This research introduce the leakage detection method to measure the electrical resistance of the cracked prototype pipe installed ECP gasket. Also the Arduino program is set to monitor the incident of chemical leakage.

As a part of an infrastructure project on medicinal herb-based remedies, we conducted a phytohemical investigation of the 100% MeOH extract from the aerial part of Boehmeria quelpaertense; our findings resulted in the isolation of flavonoids (1-2), isoquercitrin (1) and hyperoside (2). The identification and structural elucidation of these compounds were based on 1H-,13C-NMR, and LC ESI IT-TOF MS data. All the compounds isolated from this plant were reported for the first time. In this study, we examined the antioxidant activity of the 1 and 2 on the hydrogen peroxide (H2O2)-induced oxidative stress in a Rat Cardiomyoblast cell line (H9c2). The pretreatment of the flavonoids showed that it protects against H2O2-mediated cell death in the H9c2 cell line. Also, it decreases the intracellular reactive oxygen species (ROS) levels by the flavonoids in the H2O2-treated H9c2 cell line. These results showed that the 1 and 2 are a source of antioxidants. As a result, they might be helpful in preventing the progress of various oxidative stress mediated diseases, including myocardial infarction.

Background : Korean Indigenous Hylotelephium erythrostictum is widely distributed in South Korea and is used in Korean traditional medicine. In this study, the phylogenetic analysis of Korean native Hylotelephium erythrostictum and related plants on Internal Transcribed Spacer (ITS) sequences were investigated to distinguish its origin. Methods and Results : The phylogenetic analysis of 6 species of Hylotelephium were investigated by ITS. The dendrogram was constructed by UPGMA(Unweighted Pair Group Method with Arithmetic Mean) clustering algorithm based on genetic similarity of ITS. In the ITS sequence analysis, the size of total was varied from 676 to 779 bp. The size of ITS 1 was rated at 287bp, while ITS 2 was rated at 123bp. The G+C content of ITS region was ranged from 60 to 66%. In the ITS tree, six species of Hylotelephium were monophyletic, and H. viviparum was the first branching within the clade. Conclusion : H. spectabile formed a clade with H. erythrostictum, while H. verticillatum formed with H. viviparum.

Background : Agrimonia pilosa (A. pilosa) Ledebour has been registered in The Korean Herbal Pharmacopeia (KHP). In the recent study, A. Coreana showed antioxidant and anti-inflammatory effect. However, Studies of components in Agrimonia coreana (A. Coreana) Nakai was not much. So, we compared A. pilosa and A. coreana by High performance liquid chromatography (HPLC). we perfomed Thin layer chlromatography (TLC) including the anatomical characteristics by using microscope. Methods and Results : The anatomical characteristics of A. pilosa were similar to them of A. coreana. But, fascicular fivers of A. Coreana was broader than it of A. pilosa. TLC were performed to identify the Rutin and Apigenin-7-glucuronide compound. In the extract of Agrimoniae Herba, they were identified on the spot of Rf 0.2, 0.4 in Ethyl acetate - Formic acid – Water (8 : 1 : 1). The Rutin and Apigenin-7-glucuronide were analysed by HPLC/UV with Thermo Column (5 μm, 4.6 × 250 mm, C18), the column temperature at 40 ℃ and a diode-array detector (DAD) seted at 255 nm and 338 nm. The mobile phase was composed of water and acetonitrile containing 0.1% formic acid with the flow rate 1 mL/min. All compounds showed good linearity (R2>0.999) within test ranges. Agrimoniae herba was extrated by four kinds of extraction methods: MeOH, 50% MeOH, EtOH and water. The highest extraction rate occurred, when it was treated with 50% Methanol for refluxing extraction (60min). Content of Rutin was found to be 0.07±0.00 mg/g in A. pilosa and 0.02±0.00 mg/g in A. coreana. Content of Apigenin-7-glucuronide was found to be 0.12±0.00 mg/g in A. pilosa and 0.11±0.00 mg/g in A. coreana. Conclusion : The anatomical characteristics of A. pilosa were similar to them of A. coreana. Contents of Rutin and Apigenin-7-glucuronide in A. pilosa was higher than them in A. coreana slightly. But there were observed the similar patterns of Agrimonia pilosa Ledebour and Agrimonia coreana Nakai on the finger print anelysis.

Background : Campanula takesimana is a herb(Jabanpungnyeoncho) used traditionally in the korea private and we tried the development on a medicinal material. It was known that it has chlorogenic acid, as a immunoadjuvant activity. In this study we investigated the contents of the chlorogenic acid in Companula takesimana at different harversting time by the high performance liquid chromatographic(HPLC)-PDA. Campanula takesimana was collected on May maddle (Flower buds farmed), June middle (flowers opened) and July middle (seeds were mature). Methods and Results : HPLC analysis was carried out using X-bridge C18 column (5um, 250*4.6mm) and a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 1.0 ml/min. The optimum wavelength for the detection of the Chlorogenic acid was 330 nm using Photo Diode Array Detector. The contents of the chlorogenic acid in the Campanula takesimana extrat were 1.00% (May middle), 0.84% (June middle), 0.05% (July middle), respectively. As the different parts of Campanula takesimana, chlorogenic acid contents in May middle extrat were 2.73% (aerial part), 0.05% (root) and in June middle extrat were 1.61% (aerial part), 0.03% (root), 0.70% (flower) and in July middle were 0.13% (aerial part), 0.03% (root), 0.00% (seed), respectively. Conclusion : From the above results, the highes content of chlorogenic acid was observed in aerial part and May middle extract of Campanula takesimana.

Background : Curcuma longa L., is a very famous spice and medicinal herb in several countries of the world. Curcumin and volatile oils of C. longa improve hepatic and renal functions, and could be used against biliary disorders, diabetic and hepatic disorders. This medicinal plant was introduced for the regional promotion, Jeollanam-do, as the subtropic area in Korea. In the present study, the plant was introduced to the Jeollanam-do region of Korea with the aim to determine the optimal planting densities for growth characteristics, yield and curcumin contents which have not been reported up until now. Methods and Results : The field experiment was conducted at the experimental farm of Division of Traditional Korean Medicine Resource, NIKOM, Jangheung-gun, Jeollanam-do, Korea. The seedstock was planted at 40×20cm, 40×30cm and 40×40cm spacing with 3 replications. Nine plants were selected randomly for observations on growth characteristics such as plant height, stem diameter, leaves number, leaves area and to dry weight at days after transplanting (DAT) 90, 120 and 150. The dried samples of plant were stored in air tight polythene bags at room temperature for HPLC analysis of curcumin, which was undertaken following standard procedures. Top plant growth was observed in the 40×30cm planting density of C. longa, plant height, leaves number, leaves area and day shoot weight were larger than in the other treatments. Root length, root diameter and supporting roots of underground part were increased as planting density was wide, and dry root weight was increased in 40×30cm. However, yield and curcumin constituent was highest each 294 kg/10a and 68.7 mg/g in the planting density of 40×20cm. Conclusion : Based on the results of this study, planting density of 40×20cm is the most suitable in the cultivation and curcumin constituents of C. longa.