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        검색결과 19

        1.
        2012.09 구독 인증기관 무료, 개인회원 유료
        Cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. This study was designed to determine effect of glycerol and ethylene glycol as cryoprotectant in extender on improve the freezability of Jeju horse semen. The semen was cryopreserved with glucose-EDTA extender containing each 5% glycerol, 5% ethylene glycol, 8% glycerol or 8% ethylene glycol, respectively. Post-thawed sperm were evaluated motility, viability, Membrane integrity and acrosome integrity. Post-thawed sperm motility were not significantly differences among treatments. However, sperm viability were significantly higher (p<0.05) in 8% glycerol (39.85% ±11.41) than in 5% glycerol treatment (18.08%±1.61). In membrane integrity, swelling sperm ratio was significantly higher (p<0.05) in 8% glycerol (34.12%±11.02) than other treatments. In the percentage of capacitated sperm assessed by CTC staining, F pattern was significantly higher in 8% ethylene glycol than 5% glycerol and 5% ethylene glycol (p<0.05). B pattern ratio was significantly increased in 5% ethylene glycol compared with 8% glcerol and 8% ethylene glycol (p<0.05). Moreover, 8% ethylene glycol treatment was significantly decreased AR pattern ratio compared with other treatments (p<0.05). It is concluded that treatment of 8% glycerol was improved the sperm viability and 8% ethylene glycol was improved the sperm ascrosome integrity after thawing. However, they were not significantly difference between 8% glycerol and 8% ethylene glycol on post-thawed sperm viability. Therefore, 8% ethylene glycol was more effective sperm cryoprotectant than 8% glycerol in Jeju Horse.
        4,000원
        2.
        2012.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        내현적 주의와 재정향이 탐지과제 수행에 미치는 영향을 반응촉진과 회귀억제를 통해 탐색하였다. Posner와 Cohen(1980)의 고전적인 탐지패러다임에서는 단서를 제시하고 일정한 자극제시시차 후 그 단서와 같거나 또는 다른 위치에 나타나는 표적에 대한 탐지수행을 관찰한다. 본 연구에서는 내현적 주의를 유발하기 위해 단서가 표적에 대해 예측력을 가지도록 조작하였고, 단서자극과 표적 사이에 새로운 단서(재정향단서)를 삽입하여 재정향을 유도하였다. 실험 1에서는 재정향단서가 제시되는 시기를 초기, 중기, 후기로 구분하여 실험을 실시하였다. 그 결과 재정향이 제시되는 시기별로 자극제시시차(150ms, 400ms, 850ms)에 따라 다른 양상의 반응촉진 및 회귀억제가 발생하였으나, 해석 가능한 일정 패턴을 확인하기는 어려웠다. 하지만 재정향이 초기에 발생한 실험조건을 재분석한 결과, 반응촉진과 회귀억제가 자극제시시차에 따라 교차하여 나타나는 단순탐지과제의 전형적인 결과를 얻을 수 있었다. 실험 2에서는 실험 1에서 재정향이 초기에 발생하는 조건에 대한 추가 실험을 실시하였다. 실험 결과, 자극제시시차가 짧을 때는 반응촉진이 발생하였으며 자극제시시차가 길때는 회귀억제가 발생하였다. 이 결과는 단서자극이 표적에 대한 예측력을 가질 때 자극제시시차가 긴 조건에서 회귀억제가 사라진다는 기존의 보고(Wright & Richard, 2000)와 반대되는 결과이다. 이 결과는 최초 단서가 제시된 후 회귀억제의 효과가 소멸되기 전에 매우 빠르게 제시되는 재정향단서는 이중 회귀억제를 가져온다는 것을 제안한다. 본 연구는 내현적 주의에 의해 특정한 위치에 주의를 할당할 때에도 반복적으로 빠르게 제시되는 단서자극은 회귀억제를 극대화함으로써 내현적 주의를 상쇄할 수 있음을 시사한다.
        4,000원
        3.
        2011.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Our previous study demonstrated that fermented four-herb combination (Ulgum, Yagmomil, Maesil and Bokbunja) with probiotics (Saccharomyces cerevisiae, Lactobacillus acidophilus, Bacillus subtilis) (hence, this additives was designated PFH) have immune enhancing and antibacterial effects in broiler chickens. The aims of the present study were to investigate the effects of PFH on growth performance and meat quality of broiler chickens. Twenty-day-old Ross broiler chickens were fed with or without PFH for 2 weeks. Body weight gain of the 2% PFH-fed group was significantly increased compared with those of the control group (*P<0.05). Moreover, body weight gain of the 1% PFH-fed group was also showed a increased tendency compared with the control group, although the difference was not significant (P=0.06). However, no significant difference in feed intake was observed among the groups. Feed conversion ratio of the 2% PFH-fed group was significantly decreased compared with those of the control group (*P <0.05). In addition, feed conversion ratio of the 1% PFH-fed group was also showed a slightly decreased tendency compared with the control group, although the difference was not significant (P=0.07). However, no significant differences in the proximate composition (moisture, crude fat, crude protein and crude ash) and the physico-chemical index (cooking loss, shearing force, water holding capacity and pH) were found among the groups. In addition, succulence, tending and flavor in meat of all 3 groups were also not different. Taken together, these findings suggest the possibility that PFH could be used as the alternative of antibiotic growth promoters by improving the performance of broiler chickens.
        4,000원
        4.
        2007.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        더러브렛종 암말의 번식 능력을 향상시키기 위해 호르몬 투여 및 인공 조명에 의한 인위적으로 발정 조절을 실시한 결과는 다음과 같다. 발정 주기에 있는 암말의 또는 altrenogst투여에 의한 발정 발현율은 100%이었으며, 수태율은 각각 95.2% 및 71.4%였다. 전년도 공태마에 대하여 altrenogest단독 또는 altrenogest과 estradiol 병행 투여시 3월 이내 100% 발정이 발현되었으며, 발정 발현일은 altrenogest 투
        4,000원
        6.
        2016.06 KCI 등재 서비스 종료(열람 제한)
        Background: Sedum takesimense Nakai has been used as folk medicine in Korea. The present study aimed to determine the biological activity of S. takesimense by investigating the anti-inflammatory effects of S. takesimense water extract (SKLC) on the lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Methods and Results: Cytotoxicity of SKLC on RAW 264.7 cells was determinded by performing MTS assay was found to have no cytotoxic effect on RAW 264.7 cells at a concentration range of 62 - 500 ㎍/㎖. Further, pretreatment of SKLC inhibited lipopolysaccharide-induced nitric oxide (NO) production in a dose-dependent manner. To determined the inhibitory mechanisms of SKLC on inflammatory mediators, we assessed the inducible nitric oxide synthase (iNOS) and cyclooxygnease-2 (COX-2) pathways. The activities of these pathways were decreased in a dose-dependent manner by SKLC. The production of tumor necrosis factor- α (TNF-α), interleukin (IL)-1β‚ and IL-6 were also reduced. Conclusions: These results suggest that the down regulation of iNOS, COX-2, TNF-α, IL-1β‚ and IL-6 expression by SKLC are mediated by the down regulation of nuclear factor-κB (NF-κB) activity, a transcription factor necessary for pro-inflammatory mediators. This might be the mechanism underlying the anti-inflammatory effects of SKLC.
        10.
        2014.10 KCI 등재 서비스 종료(열람 제한)
        Dendrobium moniliforme (DM) is a valuable and versatile herbal medicine with the anecdotal claims of antioxidant and anti-inflammation. In the present study, we investigated the whitening and anti-wrinkling effects of DM under various conditions with B16F10 melanoma cells and human dermal fibroblasts. The DM extract inhibited melanin contents and tyrosinase activity in a dose-dependent manner, compared with untreated group. Treatment of the DM extract effectively suppressed the α-MSH-stimulated melanin formation, tyrosinase activity and dendrite outgrowth. Moreover, the α-MSH-induced mRNA expressions of tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2) and protein expression of tyrosinase were significantly attenuated by DM treatment. We also investigated the DM increased the production of type I procollagen and inhibited TNF-α-induced mRNA expressions of MMP-1, -3 in the human dermal fibroblast. These results indicate that DM may be a great cosmeceutical ingredient for its whitening and anti-wrinkle effects.
        14.
        2013.06 KCI 등재 서비스 종료(열람 제한)
        This study was carried out to find out the optimum method of preparation of indigo standard solution and its stability, and to investigate the indigo contents in Niram, blue dye extract, from a total of 7 indigo plants and 34 breeding lines of Persicaria tinctoria H. Gross. Proper solvent for indigo standard was dimethyl sulfoxide (DMSO), and appropriate concentration was 1 mg of indigo in 10 mL of DMSO. Absorbance value of UV/Vis Spectrophotometer at 620 nm of standard solution was changed decreasingly 12 hours after the preparation of standard solution irrespective of the storage conditions such as temperature and light. Average value of absorbance of 8-fold diluted standard solutions prepared daily during 16 days was 0.210±0.005, indicating the powder of indigo compound was stable chemically. Calibration curve was made for quantitative analysis of indigo of 7 Niram samples, and indigo contents ranged from 0.69% to 18.76% showing relatively larger variation. Across all 34 breeding lines, the range of indigo content was from 7.9 mg to 56.4 mg per 100 g of fresh leaves, averaging 25.2 mg of indigo content and showing a 47.7% coefficient of variation.
        15.
        2013.06 KCI 등재 서비스 종료(열람 제한)
        쪽 두 품종, 나주재래종과 나람블루를 사용하여 높은 생엽수량 및 색소함량을 얻기 위한 적정한 이식시기와 수확시기를 알아보고자 수행한 재배시험 결과, 대체적으로 두 품종 모두 비슷한 경향을 나타냈다. 이식시기가 늦을수록 생엽수량은 감소하는 경향을 보였으며, 이식시기 시험구간 다소 차이가 있었지만 니람이나 색소함량은 유의한 차이를 보이지 않았다. 수확시기가 늦어질수록 초장, 분지수, 생엽수량은 증가하는 경향을 보였지만, 니람함량은 8월 20일까지증가하다가 이후 정체하거나 감소하는 경향을 보였다. 수확시기에 따른 인디고 함량 변화는 8월 5일에 가장 높은 함량을 보였고 이후 감소하는 경향을 보였다. 따라서 쪽 재배에서 높은 생엽수량과 색소함량을 얻기 위해서는 가능한 이르게 이식하는 것이 좋으며 8월 초순경에 수확하는 것이 유리한 것으로 판단되었다.
        19.
        2003.08 KCI 등재 서비스 종료(열람 제한)
        하천에 오염물질이 순간적으로 유입된 경우에는 연속적인 유입의 경우와 다르게 분산계수의 변화에 따라 오염물질의 거동 특성이 민감하게 변한다. 하천에 순간적으로 유입된 오염물질의 거동특성을 분석하기 위하여 한강하류부에서 수리인자 및 수질인자를 실측하였다. 분산계수 추정에 사용되는 경험식에 실측된 수리인자를 적용하여 갈수시 한강하류부의 분산계수의 규모를 분석하고, 적용 가능성이 큰 경험식을 제시하였다 또한, 실측된 수질인자를 RMA-4 모형의 계산치와 비교하