Background : Korean Indigenous Hylotelephium erythrostictum is widely distributed in South Korea and is used in Korean traditional medicine. In this study, the phylogenetic analysis of Korean native Hylotelephium erythrostictum and related plants on Internal Transcribed Spacer (ITS) sequences were investigated to distinguish its origin. Methods and Results : The phylogenetic analysis of 6 species of Hylotelephium were investigated by ITS. The dendrogram was constructed by UPGMA(Unweighted Pair Group Method with Arithmetic Mean) clustering algorithm based on genetic similarity of ITS. In the ITS sequence analysis, the size of total was varied from 676 to 779 bp. The size of ITS 1 was rated at 287bp, while ITS 2 was rated at 123bp. The G+C content of ITS region was ranged from 60 to 66%. In the ITS tree, six species of Hylotelephium were monophyletic, and H. viviparum was the first branching within the clade. Conclusion : H. spectabile formed a clade with H. erythrostictum, while H. verticillatum formed with H. viviparum.