본 연구에서는 고투과성 Ni-ZiO2 모세관 지지체를 NIPS 방법에 의해서 제조되었으며, 기체분리용 고투과성 분리막 제조에 사용하고자 하였다. 이때 Ni:ZrO2 비율을 각각 100:0, 70:30, 50:50, 30:70으로 섞어 만든 도프용액을 이용하여 모세관 지지체를 제조한 후 소결온도를 950, 1000, 1100, 1200, 1300, 1400, 1500°C으로 달리하여 Ni:ZrO2 비율과 소결온도가 Ni-ZiO2 모세관 지지체의 기계적 안정성, 기공구조 및 단일기체투과도에 미치는 영향을 분석하고자 하였다.

본 연구의 목표는 유자박 올레오레진, 비타민, 미네랄이 첨가된 유자 젤리 제조 시 설탕 및 자일리톨의 함량 차이가 유자 젤리의 외관 및 물성에 미치는 영향을 연구하는 것이다. 유자 젤리는 유자 착즙액에 젤라틴, 유자박 올레오레진, 비타민, 미네랄을 첨가하고 이때 설탕 및 자일리톨의 조성을 각각 0, 25, 50%의 조건에서 제조하였다. 제조된 유자 젤리의 특성분석을 위하여 명도, 적색도, 황색도를 측정하였고, 저장 기간에 따른 유자 젤리의 견고성, 점성, 탄성, 점착성 변화를 측정하였다. 유자 젤리는 설탕의 첨가 비율이 높을수록 명도가 낮고 견고한 젤리가 제조되었고, 유자박 올레오레진을 첨가하였을 때 명도가 낮고 부드러운 젤리가 제조되었다. 유자 젤리는 저장 기간이 증가함에 따라 설탕보다 자일리톨의 첨가 비율이 높은 시료에서 당 석출 정도가 높았다. 결론적으로 설탕과 자일리톨의 비율이 각 25%일 때 유자 젤리의 외관 및 물성 변화가 최소화되었다. 본 연구에서 확립한 유자 젤리 제조 방법은 유자박의 활용성 제고 및 고부가가치 유자 가공품 개발을 위한 기초 자료로 사용될 수 있을 것으로 판단된다.

본 연구에서는 자포니카 타입 품종 중 하나인 설갱을3가지(100, 200, 300mesh)입도크기로 나눠 제분하여 글루텐 프리 쌀 식빵을 제조하고 빵의 부피, crumb의 경도, 색도, 외관형태를 통해 제빵 적성에 가장 적합한 입도를 결정하였다. 100mesh를 통과한 쌀가루로 제조한 쌀 식빵의경우 기공이 일정하지 않고 crust의 갈라짐 현상이 발생하였고, 300mesh를 통과한 쌀가루로 제조한 쌀 식빵의 경우반죽을 형성하기 위한 물에 의해 발효과정이 적절하지 않게 진행되어 반죽이 부풀지 못해 좋지 않은 품질의 빵이제조되었다. 200mesh 체를 통과하여 제조된 쌀 식빵의 경우 가장 큰 비체적과 낮은 crumb의 경도, 균일한 기공모양등 제빵 적성에 적합한 것으로 나타났다. 쌀은 밀에 비해쌀알의 경도가 높아 미세한 입자크기를 갖도록 제분할 때전분에 손상이 과하게 발생하는 단점이 있는데, 이와 달리설갱 품종은 일반 쌀 품종에 비해 쌀알의 경도가 낮아200mesh 체를 통과하는 미세한 쌀가루를 제조하는 과정에서도 전분의 손상도가 낮게 제분할 수 있는 특징을 보였다.이러한 연구결과를 통해 글루텐 프리 쌀 식빵을 제조하는데 있어 가장 적합한 쌀가루의 입도를 결정할 수 있다.

본 연구에서는 자포니카 타입 3품종(설갱, 고아미, 백진주)의 쌀을 이용하여 글루텐 프리 쌀 식빵을 제조하고 제빵 가공적성을 빵의 부피, TPA, crust의 색도를 통해 평가하였다. 품종별 특성을 보았을 때 손상전분의 함량은 4.5% 이내, 아밀로스 함량은 20-25% 일 때 가장 적합한 것으로 확인되었는데, 3종류의 품종 중 설갱으로 식빵을 제조했을 때 부피가 가장 크고, 기공 분포 상태가 양호하며, crumb의 경도가 낮아 식빵을 제조하는데 가장 적합한 품종인 것으로 나타났다. 설갱은 쌀알의 경도가 다른 품종에 비해 낮아 분쇄가 쉽게 이루어질 수 있는데 이러한 설갱의 특징은 손상전분의 함량이 적게 미분할 수 있으며 중간 정도의 아밀로스 함량을 가지는 품종에 특성상 제빵적성에 적합한 것으로 생각된다. 이러한 결과를 통해 쌀 식빵을 제조하는데 있어 설갱이 제빵적성에 가장 부합하는 품종이며 이를 활용하여 쌀 식빵을 제조했을 때 가장 좋은 품질의 식빵을 얻을 수 있을 것으로 기대된다.

This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The semen was collected using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by Tris-Egg yolk extender and contrifuged in 1,500 rpm for 15 minutes. The supernatant was removed. The pellect was diluted to final sperm concentration of 2×108/ml by doubling in every 30 minutes at 4℃ cold chamber. The semen was equilibrated for 4 hours at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm for 10 minutes. The height and duration affect the freezing speed by temperature. The frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at 37℃ water bath. Frozen-thawed sperm were evaluated sperm viability, membrane integrity and acrosome integrity. Post-thawed sperm viability has been significantly higher (p<0.05) in fresh sperm (93.27±1.62%) than frozen-thawed sperm (73.34±3.27%). However, there were no significant differences between fresh and frozen-thawed dead cell rate (7.35±2.63 vs, 13.71±2.85). In sperm motility, between Triladyl and AndroMed Extender, there was no significant different (72.86±2.83 vs, 81.47±2.48), similarly, the dead cell rates was similar (18.41±3.42% and 17.26±4.25). The results of our study suggest that AndroMed to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Jeju Native Black bull semen.

데니쉬 페이스트리의 유지는 반죽과 결합하여 수분증발속도를 늦춰주고 부피를 증대시키는 효과가 있으며 유지의 종류에 따라 데니쉬 페이스트리의 품질이 다르다. 유지의 종류에 따라 다른 데니쉬 페이스트리의 특성을 비교한 결과, 쇼트닝을 충전용 유지로 이용한 데니쉬 페이스트리는 가장 작은 부피와 가장 큰 경도와 점탄성을 보여주었으며 단면을 잘라 비교하였을 때 상대적으로 크고 불규칙한 기공을 나타내었다. 반면 버터를 충전용 유지로 이용한 데니쉬 페이스트리는 비교적 큰 부피와 작은 경도를 갖는 것을 보아 매우 부드러운 질감을 가졌으며 조밀하고 균일한 크기의 기공을 나타냈다. 따라서 버터, 마가린, 쇼트닝을 이용하여 데니쉬 페이스트리를 제조 시 버터를 이용하였을때 가장 좋은 품질의 데니쉬 페이스트리를 제조할 것으로 기대된다.

Cryopreservation induces sublethal damage to the spermatozoa, which leads to their reduced fertile life. This study was designed to determine effect of glycerol and ethylene glycol as cryoprotectant in extender on improve the freezability of Jeju horse semen. The semen was cryopreserved with glucose-EDTA extender containing each 5% glycerol, 5% ethylene glycol, 8% glycerol or 8% ethylene glycol, respectively. Post-thawed sperm were evaluated motility, viability, Membrane integrity and acrosome integrity. Post-thawed sperm motility were not significantly differences among treatments. However, sperm viability were significantly higher (p<0.05) in 8% glycerol (39.85% ±11.41) than in 5% glycerol treatment (18.08%±1.61). In membrane integrity, swelling sperm ratio was significantly higher (p<0.05) in 8% glycerol (34.12%±11.02) than other treatments. In the percentage of capacitated sperm assessed by CTC staining, F pattern was significantly higher in 8% ethylene glycol than 5% glycerol and 5% ethylene glycol (p<0.05). B pattern ratio was significantly increased in 5% ethylene glycol compared with 8% glcerol and 8% ethylene glycol (p<0.05). Moreover, 8% ethylene glycol treatment was significantly decreased AR pattern ratio compared with other treatments (p<0.05). It is concluded that treatment of 8% glycerol was improved the sperm viability and 8% ethylene glycol was improved the sperm ascrosome integrity after thawing. However, they were not significantly difference between 8% glycerol and 8% ethylene glycol on post-thawed sperm viability. Therefore, 8% ethylene glycol was more effective sperm cryoprotectant than 8% glycerol in Jeju Horse.

This study was designed to determine whether low-density lipoporoteins (LDL) extracted from egg yolk in extender improve the function of Korean Jeju Black Bull semen. The semen was cryopreserved with 5% ethylene glycol (EG) or 7% glycerol (G) extenders containing 10% egg yolk (EY), 4% LDL and 5% EY or 8% LDL. Frozen-thawed sperm were evaluated sperm motility, viability, membrane integrity and acrosome integrity. Post-thawed sperm motility has been significantly higher (p<0.05) in 4% LDL + 5% EY (; EG and ; 7% G) than 8% LDL (; EG and ;G). Treatment of 4% LDL + 5% EY-EG () has been significantly improved sperm viability compared to other treatments except 10% EY - EG. Moreover, in membrane integrity, swollen sperm ratio has been only significantly increased (p<0.05) in 4% LDL + 5% EY - EG () among all treatments. In assess to detect acrosome integrity, especially, AR pattern ratio has been significantly decreased (p<0.05) in 4% LDL + 5% EY - EG among all treatments. In sperm viability as time passes, between 4% LDL + 5% EY and 10% EY, there was no significant difference, but 8% LDL was significantly decreased sperm viability in EG (1 and 2 hrs) and G (30 min, 1, 2, 5 and 12 hrs) extender. However, there were no significant differences among all treatments except 8% LDL-G in sperm membrane integrity. 8% LDL-G has been significantly decreased swollen sperm ratio at 5 hrs after thawed. It is concluded from these results that 4% LDL + 5% EY to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Korean Jeju Black bull.

This study was designed to determine whether low-density lipoproteins (LDL) from egg yolk and taurine, hypotaurine and trehalose as antioxidant in extender improve the freezability and fertility of Korean Jeju Black Bull semen. The semen was cryopreserved with tris egg yolk extenders containing 7% glycerol and treated 4% LDL, 20 mM taurine, hypotaurine and trehalose. Frozen-thawed sperm were evaluated motility, viability, membrane, and acrosome integrity and sperm penetration ability. The results were compared to semen cryopreserved in tris egg yolk extender only as control. Frozen-thawed semen evaluation cleary indicated that the addition of LDL and LDL-antioxidants (taurine, hypotaurine and trehalose) combination were significantly improved (p<0.05) the viability (%; with staining test using eosin-Y) compared to control spermatozoa. Also, in membrane integrity (%; with supravital hypo-osmotic swelling test), not only LDL-antioxiants combination but also LDL were significantly increased (p<0.05) the swelled sperm using HOST compared to control. Sperm acrosome integrity state was classified by CTC (chlortetracycline) staining test. F pattern was significantly increased in LDL-antioxidant combination than control (p<0.05) and B pattern was not significantly differences among all treatments and control. However, AR pattern was significantly decreased in LDL-antioxidants combination than control (p<0.05). Pronucleus formation and sperm penetration index (SFI) were significantly increased in LDL and LDL-antioxidants combination than control (p<0.05). Especially, LDL-taurine significantly improved pronucleus fomation and SFI than LDL (p<0.05). It was concluded that LDL and LDL-antioxidants in extender improved the freezability and fertility of Korean Jeju Black bull spermatozoa.

쌀가루를 온도와 수분함량을 조절하여 압출 한 쌀가루의 특성과 밀가루와 7:3의 비율로 혼합된 쿠키의 품질을 경도, 색도, 관능평가를 실시하여 쿠키에 적용가능성을 확인하였다. 압출 쌀가루는 호화가 이루어져 α 화 되었으며 압출온도가 100oC에서 130oC로 증가할수록 점도가 급격하게 감소되었으며 수분함량이 25%에서 27%로 증가할수록 점도가 다소 감소되는 것을 확인하였다. 압출쌀가루의 수분흡수력, 수분용해도, 팽윤력은 대체적으로 높게 측정되었으며 30oC와 80oC에서 수분용해도와 팽윤력은 생쌀가루에 비해 높게 측정되었으나 80oC에서는 130oC 압출쌀가루는 생쌀가루와 비슷하였다. 압출 공정 온도가 증가할수록 수분용해도는 급격하게 증가하였다. 밀가루와 각 압출쌀가루가 혼합된 쿠키는 밀가루 쿠키와 비교하여 경도가 높았으며 퍼짐성지수는 낮았다. 100oC 압출쌀가루가 첨가된 쿠키는 색이 짙었으며 130oC 압출쌀가루가 첨가된 쿠키는 밀가루와 비슷하였다. 압출쌀가루가 첨가된 쿠키는 관능 평가에서는 향기, 맛, 전체적인 기호도가 높았으며, 부드러운 정도는 비슷한 평가를 받았다. 압출쌀가루를 사용하여 밀가루 함량을 감소시키기 위한 연구를 실시한 결과, 압출쌀가루는 밀가루 함량을 줄여 사용할 때 가공적성문제점을 보완할 수 있었다. 또한 다양한 분야로 압출쌀가루 연구를 통해 쌀의 활용을 확대될 가능성이 있음을 확인하였다.

The objective of this study was to examine effect of ethylene glycol for semen cryopreservation in Korean Jeju Black Bull. The semen was cryopreserved with extenders containing cryoprotectants (7% glycerol and 3%, 5%, 7% ethylene glycol) and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 min, 5 cm for 10 min and 8 cm for 10 min. And then frozen straw was plunged into LN2. Post-thawed sperm motility, viability and membrane integrity were significantly higher in 5% ethylene glycol (72.5±5.00%, 54.88±0.66% and 46.00±2.40%; p<0.05). Motility and viability were similar between 7% glycerol and 5% ethylene glycol. However, the membrane integrity was significantly higher in 5% ethylene glycol (34.69±4.64% vs 46.00±2.40%; p<0.05). The viability and membrane integrity were significantly higher in 5 cm for 10 min and 8 cm for 10 min than 3 cm for 5 min (viability: 55.81±2.94, 55.19±3.34 vs 47.94±3.48%; p<0.05 and membrane integrity: 44.94±3.51, 46.06±2.25 vs 40.38±1.03%; p<0.05). The percentage of capacitated sperm assessed by CTC staining, percentage of F pattern was higher in 7% glycerol, 5% and 7% ethylene glycol, and AR pattern was significantly higher in 3% ethylene glycol. F pattern was significantly increased in 5 cm for 10 min and 8 cm for 10 min (p<0.05), but AR pattern was significantly increased in 3 cm for 5 min (p<0.05).

The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.

This study was carried out to establish most suitable freezing condition, to evaluate the different glycerol concentration of freezing and thawing rates on motility, viability, membrane integrity and acrosome intecrity of frozen Korean Jeju Black Bull spermatozoa, Semen was collected from a Korean Jeju Black Bull using an artificial vagina and transported to the laboratory. The semen was extended gradually 1:5 then cooled slowly for 2 hrs to 4. The semen was diluted 1:1 with cryoprotectant extenders (3%, 5% and 7% glycerol) and equilibrated for 2 hrs at cold chamber and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 min and above 8 cm for 10 min. And then the frozen straw was plunged into LN. The presented straws were examined the viability and motility after thawed at 37 water bath. The viability and membrane integrity immediately post-thawing were significantly higher in samples frozen in 7% glycerol than 3% and 5% glycerol (p<0.05). After CTC staining to assess acrosome integrity, F pattern was significantly increased, but B pattern was significantly decreased in 7% glycerol (p<0.05). Freezing distance of 5 cm from liquid nitrogen and pre-cooling for 10 min yield better survival and membrane integrity, but not significant difference. However, AR pattern according to CTC staining was significantly decreased in 3 cm for 5 min.

The present study investigated the efficient methods to produce in vitro Hanwoo embryos, and to improve the pregnancy rate. The developmental rate, total cell number and ICM ratio of in vitro embryos were compared amongst different culture media. Comparisons were also made on the status of recipients, pregnancy rate along with day of transfer after the estrus. Development of embryos into blastocyst stage in IVMD101 supplemented with 5% fetal bovine serum (FBS) group was significantly higher (34.2%) than that of TCM-199 supplemented with 5% FBS (26.8%) and IVMD101 without FBS (25.9%) (p<0.05). The development rate to blastocyst stage was significantly faster in IVMD101(5% FBS) than that of other groups () (p<0.05). The average number of inner cell mass and trophectoderm were similar among treatment groups, which were and . However, total cell number in IVMD101(5% FBS and 0% FBS) was significantly higher than that of TCM199(5% FBS). There were no differences in the pregnancy rate among treatment groups (32.0%, 33.9% and 28.6%, respectively). However, the pregnancy rate of Day 6 embryos cultured in IVMD101(5% FBS) was significantly (p<0.01) higher than IVMD101 without FBS and TCM-199 + 5% FBS (38.0% vs. 17.2% and 32.4%, respectively). No significant difference was observed for the pregnancy rate between heifer and cow transferred with Day 6 embryos cultured in IVMD 101(5% FBS) (42.7% and 39.3%, respectively). However, there was a significant difference of pregnancy rate (p<0.05) in heifer between one and two embryos transferred (31.4% and 41.9%). There was no difference of pregnancy rate among transfer days after estrus between heifer and cow, but the pregnancy rate of transfer to heifer with day 6 after estrus was significantly higher (p<0.05) than that of day 7 and 8 (22.2% vs. 49.0% and 38.7% respectively). Based on the above findings, there is a possibility to produce in vitro produced embryos cultured in IVMD101(5% FBS) showed higher blastocyst rate and the increased cell number. In terms of the pregnancy rate of in vitro produced embryos, the highest pregnancy rate was observed when two embryos were cultured in IVMD101(5% FBS) and transferred.

본 연구는 도축된 제주마의 난소를 이용하여 초음파 유도난포란 채취 기술을 확립하고자 난소, 난포 및 황체의 크기를 초음파상과 육안적 측정치를 비교하고자 하였다. 초음파상 측정치와 caliper를 이용한 실제 크기의 측정치 비교에서는 통계학적 차이는 보이지 않았다. 배란 직전의 난포를 조사한 결과, 초음파상에서 관찰한 것은 난소 한 개 당 평균 0.83개와 평균 크기는 2.86 m였으며, 육안으로 관찰된 것은 난소 한 개 당 평균 0.75개와 평균 크기는

본 연구에서는 제주마의 연령에 따라 Caslick's index와 번식률의 관계를 조사하고자 수행하였다. 제주마의 연령이 증가할수록 Caslick's index 역시 증가됨을 알 수 있었다. 연령이 어린 암말(세)에서는 평균 Caslick index가 45.4, 세에서는 72.5, 15세 이상은 72.5로 나타났다. Caslick's index에 따른 암말의 평균 연령은 Caslick's index 50 이하에서는 10.9세이고, 50이상 150 이하에

본 연구에서는 수컷 랫드에 DEHP를 투여하여 실험적으로 생식 독성을 유발하고, vitamin E와 catechin을 단독 및 병용 투여하여 수컷 랫드에서 정소의 조직학적 변화, 정액 특성의 변화 및 정자의 운동성 변화 등을 조사하여 그 예방 효과를 알아보고자 하였다. DEHP를 투여한 군은 대조군에 비해 정자농도, 정자 생존율, 정상 정자율이 감소하였다. DEHP에 의한 생식 독성을 예방하기 위해 vitamin E와 catechin을 단독 또는 병행

본 연구는 제주도의 흑우에서 다배란을 유기한 후 발정기 동안 혈중 호르몬의 농도 검사, 혈액 생화학 검사, 체내 수정란 회수율 등을 관찰하였다. FSH를 4일간 8회 50 mg씩 주사하여 다배란을 유기하였다. 성호르몬의 측정은 radioimmunoassay (RIA)법으로 측정하였으며 혈액 생화학치는 자동혈청 분석기로 측정하였다. 인공 수정 후 7일에 수정란을 비외과적 방법으로 회수하였다. 본 연구의 결과는 다음과 같았다. 1. 혈중 호르몬의 농도를 측