탁주 및 약주의 양조시에 koji를 곰팡이로 사용되고 있는 Aspergillus awamori var. kawachii로서 쌀 koji를 제조할 때에 방화효소의 생산에 영향을 미치는 배양조건을 검토하였다. 본 균의 당화효소 생산 최적온도는 36℃이었고, 이 온도에서 40시간 배양했을 때 최고값에 도달하였다. 배양개시 후 20∼25시간까지 36℃로 배양한 후 그 이후에 32℃로 배양하는 방법은 koji의 유기산 생산과 당화효소 생산에 호의적이었다. 증미의 수분함량이 35% 이하일 때는 당화효소의 생산이 낮았으나 40∼60% 일 때는 높았다. 국균포자의 접종량이 지나치게 적을 때에는 배양초기의 당화효소 생산은 낮았으나 배양 40시간 이후에서는 차이가 없었다. Koji의 두께가 3㎝ 이상일 때는 당화효소의 생산이 현저히 억제되었다. 본 koji의 당화효소는 pH 2∼7에서 안정하였고, pH 2∼5에서 높은 활성을 나타내었다.

Concern about water quality and pollution was heightened as contaminants flowed into the Namhan River downstream of the Chungju Dam. Thus, this study calculated the Real-Time Water Quality Index (RTWQI) using monthly average water quality monitoring data for major tributaries that require water quality control, which were obtained between 2010 and 2019. To review the applicability of the RTWQI for water quality assessment in the major tributaries of the Namhan River, it was compared with the living environment standard river. The calculated RTWQI result indicated that Bokhacheon, with 59 points, was in the “fair” grade, and this stream was identified as a tributary that preferentially requires water quality improvement as it showed a decreasing (-) tendency of RTWQI. A comparison between RTWQI and the living environment standard of T-P showed that the categories of “completely coincident” and “different by one grade” accounted for 45% of the total, and the water quality of major tributaries was assessed as low. Therefore, the RTWQI, which applies comprehensive water quality parameters and has higher reliability than assessing a single water quality parameter, was determined to be efficient for water quality assessment of the major tributaries of the Namhan River.