10% ethanol에 의한 처녀발생유가 및 체외수정된 돼지 난포란을 CZB와 CRlaa 에서 배양하여 배발달율을 조사하였다. 또한 CZB에 각기 다른 농도의 cholesterol (0g/mL, 2g/mL, 5g/mL, 10g/mL)을 첨가한 후 체외수정된 돼지 난포란을 배양하여 배발달률을 조사하였다. CZB 구는 BOEC와 공배양하였다. 처녀발생유가 48시간 후 2~8세포기로 발달한 난자의 비율은 CZB 구가 32.2%, CRlaa구가 16.8%였으며

3.3kb 웅성특이 DNA(pEM39 plasmid DNA)가 성 특이 DNA 검색자로 활용되어질 수 있는가를 확인하기 위하여 구조적인 분석을 Southern blotting, DNA sequencing과 computer program 분석을 통하여 실시하였다. 전체 3.3kb에서 유래된 약 1kb 단위의 단편을 이용하여 표지된 짧은 DNA probe들은 Southern blot 분석에서 웅성특이성을 나타내었다. McGraw와 Jeon의 sequence

In order to compare the forage value and to promote the growth without the hindrance of rice transplanting period for several forage crops as prior crops of paddy field, this experiment was conducted. Three kinds of foliar application, such solution as Urea, Gibb. and Gibb. +Urea were applied to promote the growth. The results gained are summarized as follows; 1. According to the forage crops, there were large difference of yields. The rankings of yield were shown as follows; Fresh weightㆍDry weight : Oats. Italian rye grass. White clover. Corn. Soybean. Crude protein content: Soybean. White clover. Corn. Italian rye grass. Oats. Crude protein Yield: White clover. Italian rye grass. Oats. Corn. Soybean. 2. The ranking of suitable forage crops as prior crops of paddy field were Oats, Italian rye grass, White clover, but Corn and Soybean were unsuited. 3. In general, length of stem, length and width of leaf were enlarged such rank as foliar application of Urea, Gibb. and Gibb. + Urea solution. There were apt to be increased fresh and dry weight by growth of stem length, these trend were shown among all crops and between foliar applications of same crops. 4. All crops were shown not only promoted growth but also increased yield by every treatments. The order of application effects were as follows; Fresh weigthㆍDry weigth: Gibb., Gibb. +Urea, Urea. Crude protein content and yield: Urea, Gibb, Gibb. + Urea. 5. In Oats, heading date was more accelerated 1, 2 and 3 days than control by foliar application of Urea, Gibb. and Gibb. + Urea respectively.