In this paper, we describe a case of using robot technology in child studies to examine children’s judgement and reasoning of the life phenomenon on boundary objects. In order to control the effects of the appearance of the robot, which children observe or interact directly with, on the children's judgement and reasoning of the life phenomenon, we developed a robot similar to human. Unit experimental scenarios representing biological and psychological properties were implemented based on control of robot's motion, speech, and facial expressions. Experimenters could combine these multiple unit scenarios in a cascade to implement various scenarios of the human-robot interaction. Considering that the experimenters are researchers of child studies, there was a need to develop a remote operation console that can be easily used by non-experts in the robot field. Using the developed robot platform, researchers of child studies could implement various scenarios by manipulating the biological and psychological properties of the robot based on their research hypothesis. As a result, we could clearly see the effects of robot's properties on children’s understanding about boundary object like robots.

골 형성 단백질(Bone morphogenetic protein, BMP)은 TGF-β superfamily의 구성원 중에 하나이며, 이 들은 원래 뼈 형성을 유도하는 능력에 의해 발견되었지만, 곧 세포분열, 세포자살, 세포이동 그리고 세포 분화를 조절하는 것으로 밝혀졌다. 매우 다기능적인 골 형성 단백질은 포유동물의 reproduction에도 매우 중요한 역할을 하지만 알려진 바가 적다. 그래서 본 연구에서는 골 형성 단백질이 착상 전 oviductal environment에 어떠한 영향을 미치는지 밝히고자 하였다. 먼저, Estrus 시기 생쥐의 oviduct cDNA를 통해 RT-PCR한 결과, BMP1, 2, 3, 4, 5, 6, 7, 8, 10, 15 mRNA와 BMP receptor인 BMPR1A, BMPR1B, BMPR2 mRNA의 발현을 확인하였고, BMP Inhibitor인 Noggin, Follistatin, Chordin mRNA의 발현 또한 확인하였다. 이들 중 BMP2, 3, 7, 15 mRNA의 level이 BMP5, 6, 10 mRNA의 level보다 많은 발현량을 보였다. BMP receptor 중에서는 BMPR2 mRNA의 level이 제일 높았다. BMP Inhibitor 중에서는 Chordin mRNA의 발현량이 제일 많았다. 이들 가운데 가장 높은 발현 수준을 보인 BMP2의 단백질의 localization은 Immunohistochemistry를 통해 확인하였다. BMP receptor와 BMP Inhibitor에 대한 연구는 진행 중에 있지만, 생식주기별 생쥐의 oviduct 내에서 BMP2 단백질의 발현수준은 proestrous, metestrous, diestrous보다 estrous stage에서 가장 강한 발현을 보였다. 이는 BMP2가 steroidogenesis에 영향을 주어 oviductal cell proliferation에 관여한다는 것을 보여준다.

The object of this study was to obtain single oral dose toxicity of Arisaema Rhizome (Arisaema amurense f. serratum (Nakai) Kitag) aqueous extracts. Arisaema Rhizome (Chunnamsong in Korean) is one of the most important folk remedy plants used in Asia. In the study, a 28-day rat oral gavage study has been conducted with the extracts from Arisaema Rhizome at dose of 1,250, 2,500 and 5,000 ㎎/㎏/day. The following endpoints were evaluated: clinical observations, body weight, gross and microscopic pathology, clinical chemistry, and hematology. Based on the analysis of these endpoints, it was estimated that NOEL (no observed effect level) for male rats and NOAEL (no observed adverse effect level) for female rats are 5000 ㎎/㎏/day of the water-extracts from Arisaema Rhizome.

In this study the urban atmospheric environment map in Busan was made and it consist of the atmospheric environment element map and the atmospheric environment analysis map. The atmospheric environment element map covered the topography, the urban climate, the air pollutant emission, ozone and PM10 concentrations in Busan and the atmospheric environment analysis map included the thermal environment and the wind flow by using WRF meteorological numerical simulation. The meteorological elements from 2007 to 2011 in Busan were used in this study. As a result, in the center of Busan and Buk-gu along to the Nakdong river was the temperature high. To analyze the air flow of Busan 3 clusters depending on the wind direction were extracted with the cluster analysis. The results of the analysis on the detailed wind field of each cluster showed that the weak ventilation could be happened locally at the specific meteorological condition.

PM10 concentration is related to the meteorological variables including to local and synoptic meteorology. In this study the PM10 concentrations of Busan in 2007~2011 were analyzed and the days of yellow sand or rainfall which is more than 5 mm were excluded. The sections of PM10 concentration were divided according to 10-quantiles, quartiles and 90-quantiles. The 90-quantiles of daily PM10 concentration were selected as high concentration dates. In the high concentration dates the daily mean averaged cloudness, mean daily surface wind speed, daily mean surface pressure and PBL height were low and diurnal variation of surface pressure and daily maximum surface temperature were high. When the high PM10 dates occurred, the west and south wind blew on the ground and the west wind blew strongly on the 850 hPa. So it seemed that long range transboundary air pollutants made effects on the high concentration dates. The cluster analysis using Hysplit model which is the backward trajectory was made on the high concentration dates. As a result, 3 clusters were extracted and on the short range transboundary cluster the daily mean relative humidity and cloudness were high and PBL height was low.

Ozone is the secondary photochemical pollutant formed from ozone precursor such as nitrogen dioxide and non-methane volatile organic compounds(VOCs). The ambient concentration of ozone depends on several factors: sunshine intensity, atmospheric convection, the height of the thermal inversion layer, concentrations of nitrogen oxides and VOCs. Busan is located in the southeast coastal area of Korea so the ozone concentration of Busan is mainly affected from the meteorological variables related to the sea such as sea breeze. In this study the ozone concentrations of Busan in 2008~2010 were used to analyse the cause of the regional ozone difference in eastern area of Busan. The average ozone concentration of Youngsuri was highest in Busan however the average ozone concentration of Gijang was equal to the average ozone concentration of Busan in 2008~2010. The two sites are located in eastern area of Busan but the distance of two sites is only 9km. To find the reason for the difference of ozone concentration between Youngsuri and Gijang, the meteorological variables in two sites were analyzed. For the analysis of meteorological variables the atmospheric numerical model WRF(Weather Research and Forecasting) was used at the day of the maximum and minimum difference in the ozone concentration at the two sites. As a result of analysis, when the boundary layer height was lower and the sea breeze was weaker in Youngsuri, the ozone concentration of Youngsuri was high. Furthermore when the sea breeze blew from the south in the eastern area of Busan, the sea breeze at Youngsuri turned into the southeast and the intensity of sea breeze was weaker because of the mountain in the southern region of Youngsuri. In that case, the difference of ozone concentration between Youngsuri and Gijang was considerable.

The urban microscale wind field around the air quality monitoring station was investigated in order to check how a building complex influences it. For this study as the high density areas Jwa-dong and Yeonsan-dong monitoring sites in Busan were chosen. As the direction of inflow which is perpendicular to the building of the monitoring station was expected to cause the considerable variation of the wind field, that direction was selected. The model Envi-met was used as the diagnostic numerical model for this study. It is suitable for this investigation because Envi-met has the microscale resolution. After simulating it, on the leeward side around a building complex the decrease of flow velocity and some of vortexes or circulation area were discovered. In addition, on the edge of the top at the building and at the back of the building the upward flow was developed. If the sampling hole of monitoring site were located in this upward flow, it would be under the influence of upward flow from the near street.

비타민 vitamin E 구성 성분으로 항산화, 항암, 고지혈증 개선 등 다양한 생리활성을 나타내는 tocotrienol이 국내산 차조기에서 보고된 바, 존재 여부 확인을 위하여 국내수집 6종, 일본수집 8종 등 총 14종의 차조기 유전자원과 들깨 종실을 대상으로 HPLC 및 GC, GC/MS 비교 분석을 수행 하였으며 그 결과는 다음과 같다. 1. 순상의 HPLC 이용시 차조기에서 4종의 tocopherol 및β-tocotrienol , λ-tocotrienol 과 매우 유사한 머무름 시간의 발견되었다. 2. 그러나 동일 시료를 GC로 재분석한 결과 13개 차조기 유전자원에서 모두 4종의 tocotrienol과 일치하는 물질은 발견되지 않았다. 3. GC 이용시 δ-tocotrienol 및 λ-tocotrienol 과 유사한 머무름 시간의 peak가 검출되었으나 GC/MS를 이용하여 확인한 결과 tocotrienol이 아닌 것으로 확인되었다. 4. 이상의 HPLC 및 GC 비교분석결과에 기초할 때, 차조기에는 tocotrienol류의 vitamin E는 존재하지 않는 것으로 추정되었으며 GC를 이용한 vitamin E isomer 분석이 HPLC 이용시 발생가능한 유사 머무름시간대 물질을 tocotrienol로 판단하는 오류 방지에 효과적임을 알 수 있었다.

비타민 E를 구성하는 토코페롤(Tocopherol, T)과 토코트리에놀(Tocotrienol, T3)은 각각 alpha(a)-, beta(b)-, gamma(g)-, delta(a)- 의 이성질체가 존재하며 항산화, 항암 및 고지혈증 개선 등 효과가 뛰어난 물질로 알려져 있다. 국내에서 수집된 재래종 수도 유전자원을 대상으로 이들 성분의 함량 분포를 조사코자2 20개 품종을 선발하여 현미를 분석한 결과 평균적인 aT, bT, gT 및 총T 함량은 각각 8.3, 0.5, 1.2, 및 10.0 ug/g 이었으며, aT3, gT3, dT3 및 총 T3 함량의 평균치는 각각 6.7, 17.5, 0.7 및 24.7 ug/g이었다. 8종의 vit E 이성질체의 총 합으로 나타낸 총 vit E 함량의 최소, 평균 및 최대값은 각각 9.9, 60.3 및 34.6 ug/g 이었다. 변이계수를 기준으로 평가할 때, gT (CV=79%)의 함량 분포가 품종간 변이가 심한 것으로 나타났으며 aT 함량(CV=79%)은 가장 변이가 작은 것으로 나타났다. 분석된 220개 품종 중에서 가장 높은 aT, aT3, gT3 및 총 vit E 함량을 나타낸 품종은 각각 화성밭찰, 재래륙도, 조석조, 산도이었다.

인공산성비를 처리하였을 때 작물이 입는 피해양상 상호간의 관계를 알아보기 위하여 벼(일품벼), 콩(만리콩), 감자(대지), 고추(풍촌)에 pH 1.8, 2.3, 2.8인 인공산성비와 PH 6.0인 인공비를 출아 또는 정식 후 30일 부터 수확기까지 2주 간격(감자는 1주 간격)으로 처리하여 얻은 결과는 다음과 같다. 1. 인공산성비에 의한 잎의 가시적 피해는 벼, 콩, 고추는 pH 1.8과 2.3처리에서는 갈색 반점으로 나타났고, pH가 낮을수록 피해가 더 심했다. 감자는 어느 pH의 인공산성비 처리에서도 가시적 피해가 없었다. 2. 엽록소 함량은 벼와 감자에서는 인공산성비 처리간에 차이가 없었지만 고추와 콩에서는 인공산성비 pH가 낮을수록 잎의 엽록소 함량이 감소하였다. 3. 광합성 능력은 벼에서는 인공산성비 pH간에 차이가 없었다. 그러나 콩, 감자, 고추는 pH 2.8과 6.0에서는 차이가 없었으나 pH 2.8이하에서는 pH가 낮을수록 광합성 능력이 감소하였다. 4. 벼, 콩, 감자의 수량은 인공산성비 pH간에 차이가 없었으나 고추에서는 인공산성비의 pH가 낮을수록 과장, 과경, 1 과중, 주당 과실수, 과실 수량은 감소하였다. 5. 산성비에 대한 내성이 큰 벼와 감자에서는 가시적 피해가 다른 조사형질과는 상관이 없었다. 한편 내성이 약한 콩과 고추에서 가시적 피해는 콩의 엽록소 함량과 광합성 능력, 고추의 광합성 능력과 수량간에 부의 상관이 있었다.