포유동물 수정란의 동결보존기술은 최근 기후 변화에 따른 생물종 다양성을 보존하기 위해서 중요하게 여겨지는 연구 분야이다. 따라서 멸실 위험에 처한 동물의 개량과 증식, 보존과 복원 및 생명공학의 분야에 이르기까지 응용 기술은 다양하게 이용되어진다. 본 연구에서는 한우 수정란의 동결 후 생존성 향상을 위해서 동결 방법에 따른 체내 외수정한의 내동성을 조사하였다. 완만동결에 따른 체내 외수정란의 동결 융해 후 수정란의 재확장률은 89.6%와 81.5% 그리

This thesis is a study on the oldest feng-shui book 『Janggyeong(葬經)』. Specially, it is a focus on the feng-shui orientation method so called Sasepalryongbub(四勢八龍法) in that book. This method is to identify fortune and misfortune with the directions to be measured by a feng-shui compass. Sa(四) of Sase(四勢) is the four pieces of direction of dividing 12 parts for 360 degree circle. To divide for 12 parts is related with 12-Jiji(地支). Sa mean In(寅), Sin(申), Sa(巳), and Hae(亥). Se of Sase means a large stem of mountain. Generally, Sase are the big mountain stems of the four directions as In, Sin, Sa, and Hae. Pal(八) of Palryong(八龍) mean the 8 parts of equal division for 360 degree. It is related with Palgoe(八卦) that divide for 8 parts equally. Palgoe are Jin(震), Li(離), Gam(坎), Tae(兌), Geon(乾), Gon(坤), Gan(艮), and Son(巽). Ryong(龍) of Palryong(八龍) is a stem of mountain that is measured with 8 parts of equal division feng-shui compass. Sasepalryongbub make a connection between Sase and Palryong. When they are connected, it is a good fortune like as In(寅) and Li(離), Sin(申) and Gam(坎) or Gon(坤) or Gan(艮), Sa(巳) and Tae(兌) or Geon(乾), Hae(亥) and Jin(辰) or Son(巽). It is based on two theories. The one is 12-Unseongron(運星論), the other is one of Ohaengron(五行論) as Samhap-Ohaeng(三合五行). 12-Unseongron begins from dividing all human life affairs with 12 steps. Saeng(生), Wang(旺), Myo(墓) are the very important things among 12 steps. Saeng means starting, Wang means flourishing, Myo means keeping. Samhap-Ohaeng is made with Jiji of Saeng, Wang and Myo steps about 12-Unseongron. Saeng is the most important thing among of Saeng, Wang and Myo in Sasepalryongbub which means birth. Saeng of Jiji direction can help Palgoe direction as a counterpart. Like this, the good relation of Sase and Palryong is that Jiji direction can help Palgoe direction. For example, there are In(寅) of Jiji and Li(離) of Palgoe, can be explained as these kinds of the good relations. In view of Samhap-Ohaeng, In of Jiji is regared as Saeng of fire(火). This fire– Saeng- In as Jiji can help fire- Li as Palgoe. It can be said as a good condition that Sase and Palryong be connected like this case. This is the main content of Sasepalryongbub as being treated in this study.

한우 이력추적제에 적용되는 11개의 MS marker (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, ETH3, ETH225, BM1824 and INRA23)와 성감별을 위한 2개의 sexing primer로 조합된 하나의 Multiplex PCR set를 이용하여 모근에서 추출한 genomic DNA를 이용해 3510두의 대량 시료를 분석한 결과 3.93%의 genotyping 실패율로 성공적인 분석결과를 얻었다. 무작위교배집단으로 가 정 시 동일개체출현확률 (PI)은 1.31×10-23, 반형매교배집단으로 가정 시 동일개체출현확률 (PIhalf-sibs)은 2.52×10-16 그리고 전형매교배집단으로 가정 시 동일개체출현확률 (PIsibs)은 1.09×10-6으로 나타나 현재 사용 중인 11종의 MS marker는 범용적으로 사용하여도 무방할 것으로 재확인 되었다. 또한 생산 및 사 육단계의 생우의 경우 모근을 이용하여 DNA를 추출하는 것은 시료 채취 시 소에게 주어지는 스트레스를 최소화 시킬 수 있을 뿐만 아니라 대립유전자형 분석에 있어서 시간적, 경제적인 효율성을 높일 수 있었 다. 또한 모근 채취 부위 중 등, 배, 꼬리상부와 꼬리하부를 이용하여 검정한 결과 꼬리하부의 모근을 이 용하여 5~13가닥을 사용했을 때 최적의 분석결과를 보였다. 최종적으로 한우의 사육단계 대량 유전자형 분석에 적용 가능한 96 well 단위를 기본으로 하는 모근 DNA분리 체계를 확립하였다.

This study relates to the architectural planning principle of Gyeonghoeru pavilion, one of the major buildings in Gyeongbokgung palace. The study is concentrated on The Book of Gyeonghoeru Pavilion written by Jeong Haksun in 1865 during the reconstruction of Gyeonghoeru pavilion. The architectural planning principle of Gyeonghoeru pavilion disclosed in The Book of Gyeonghoeru Pavilion appears to be 'the method of expanding with six by six system'. 'The method of expanding with six by six system' means a way of continuously extending the number related to 6 by addition, subtraction, multiplication and division, and reflecting to architecture, having number 6 as the basic number. Number 6 means a large amount of water in East Asian philosophy, Applying 'the method of expanding with six by six system' to the architecture of Gyeonghoeru includes an intention to prevent fire in a way of a type of incantation because Korean traditional wooden buildings are we to fire. Since Gyeonghoeru is surrounded by a pond and was constructed based on the number 6 having a strength of water, it was believed that it could be safe from fire. This study compares the contents disclosed in The Book of Gyeonghoeru Pavilion with the construction of Gyeonghoeru to find out how the number 6 was applied to the overall construction of Gyeonghoeru. From the fact that the total number of km of Gyeonghoeru is 36 ($6{\times}6$), the number of pillars is 42 ($6{\times}7$), and the number of windows and doors is multiples of 6, it has been found out that the number 6 is deeply related to the overall construction of Gyeonghoeru. In addition to the fact that the construction of Gyeonghoeru can be explained by 'the method of expanding with six by six system', The Book of Gyeonghoeru Pavilion also discloses parts where Diagram of Hotu, Later Heaven Arrangement and Arrangement of 64 Hexagrams are applied. Therefrom, it has been found out that Gyeonghoeru pavilion was constructed by applying the principles of East Asian philosophy based on The Book of Changes.

본 연구는 천연기념물로 지정되어 있는 제주마의 유전자원 보존과 증식을 위한 인공수정 기술의 실용화를 위한 기초자료를 제공하고자 제주도 축산진흥원에서 사육되고 있는 제주마 종마 4두를 공시하여 Missouri style 인공질을 이용 정액을 채취하여 사정 특성 및 정액의 일반 성상을 조사하였다. 제주마는 교미를 위하여 발정 중인 암말에 사정당 2.31.8회 승가를 하며 사정시간은 27.012.5초였다. 정액의 일반성상을 조사한 결과 총 정액량은 47.82

본 연구는 phosphatidylinositol 대사의 기질 및 억제물질이 돼지 난모세포의 체외 성숙·수정에 미치는 영향에 대하여 실험을 하였다. 난모세를 inositol (250 mM) 첨가 또는 무첨가한 mTLP-PVA 배양액에서 46시간 체외배양하였다. 성숙이 완료된 난모세포는 신선 돼지정액을 이용하여 6시간 동안 mTALP-PVA 배양액에서 체외수정을 시켰다. 수정 후 6시간에 이들 난모세포를 10% 혈청을 첨가한 TCM-199 배양액에서 추가로

The purpose of this study was to investigate spatial distributions of total deposition. A total number 79 samples were collected at 17 sampling sites from September 1999 to January 2000. Total (=wet+dry) atmospheric depositions were collected by filtered deposition sampler at sampling site (the Western Part of Kyongsangnam Province). In addition, the deposition of soluble and insoluble fraction was also investigated to find a suitable simplified collection method for a long-term monitoring of total deposition. The total depositions were measured soluble amount(mm/month), insoluble amount(kg/km2/month), pH, conductivity(E.C.) and eight ionic components. The spatial distribution of deposition flux was to estimated by using a kringing analysis. The 17 sites mean fluxes of water soluble ionic components; SO42-, Cl-, NO3-, Na+, NH4+, K+, Mg2+, Ca2+ were 100.7～315.6kg/km2/month, 30.1～234.3kg/km2/month, 64.4～139.4kg/km2/month, 7.5～68.3kg/km2/month, 10.7～48.7kg/km2/month, 5.6～27.9kg/km2/month, 4.5～17.5kg/km2/month, 27.6～81.7kg/km2/month, respectively.