The corrugated steel plate culvert structure was developed and actively developed in the United States in the 1930s and was introduced into the domestic scene in the 1970s. Currently, about 1,000 corrugated steel structures were being used for expressway concrete passage culverts and waterway culverts. Compared to concrete materials that require a basic curing time, corrugated steel plates could be assembled and backfilled, so that the construction speed is fast and the economics were excellent. However, as the number of public years has elapsed, problems in maintenance have been found. Therefore, there is a growing interest in the stability of corrugated steel plate structures. Therefore, in this study, the improvement for the efficient maintenance of the corrugated steel structure constructed on the expressway was examined.

On the expressway, culvert structures serve as a channel or channel connecting villages and towns blocked by clay. The culvert structures are mainly constructed of reinforced concrete or corrugated steel sheets in various ground conditions. Most culverts are divided into aging and damage trends according to the number of public years. However, most of the damage to the culvert structures applied on the soft ground is likely to be caused by the subsidence of the ground. In addition, even if the settlement of the paper sheet is stabilized, if the road is constructed at the upper part, the culvert structure is also expanded at the time of the expansion work, and damage due to a behavior different from that before the expansion work may be found. Therefore, in this study, the behavior of the culvert structures according to the ground supporting force was analyzed. For this purpose, the causes of damage are derived through design review and field investigation.

Recently, maintenance science has been promoted and many advanced equipments have been developed such as GPR surveying, automatic gauging robot, bridge deck inspection robot. And the government - led policy on the fourth industrial revolution affects the R & D field of science and technology, and science for all fields remains a challenge. However, the equipment that has been developed so far is applied separately to each field, so that it is not possible to grasp the state of the entire structure. Therefore, in this study, the applicability of BIM and image information mapping technology in expressway maintenance was analyzed. To accomplish this, a basic analysis was conducted to identify the characteristics of the equipment developed for the current structure inspection and to build a platform to manage the collected data collectively.

A entomopathogenic ascomycetous fungus, Cordyceps militaris is distributed world wide and cultivated as a medicinal mushroom having many valuable biological and pharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and tested their characteristics such as mycelial growth, mycelial density, fruiting body formation ability, the production of perithecia. Also two opposite mating types were assayed using two sets of primers specific for C. militaris, which were amplified a 191-bp fragment for MAT1-1 and 233-bp fragment for MAT1-1. F1 hybrids resulted in well-developed perithecial fruiting bodies and their crossings were confirmed by the PCR assays. New hybrids were produced showing high quality fruiting bodies in artificial media. Mycelial growth of new strain 'Dowonhongcho 2’ was better on SDAY and at 15-25°C when it was compared with that of 'Yedang 3'. The stromata of new strain were club-shaped and bright orange-red color. Its length was 7.1 cm and the cordycepin content was 0.33% on average. In comparison with 'Yedang 3', the new strain had a yield that was 7% higher and it produced fruiting bodies which were firmer. Days of fruiting body were similar with 50 days from inoculation. This cultivar may serve as a valuable one for artificial cultivation and industrial-scale production of C. militaris.

For heaths and vitality Cordyceps sinensis has been widely used as an important medicinal mushroom. C. militaris is popular for its substitute and can be cultivated artificially. Its new strains have been improved by crossing single spore strains. A bipolar heterothallic fungus, C. militaris has two strains of compatible mating types and they can be differentiated by crossing, fruiting body formation ability and the production of perithecia. Each isolate was determined to be monokaryotic or dikaryotic on the basis of perithecial formation by fruiting tests. This process was also carried out by molecular markers for the detection of two opposite mating types or F1 hybrids using two sets of primers specific for C. militaris amplifying a 191-bp fragment for MAT1-2 and 233-bp fragment for MAT1-1. Therefore the crossing of two compatible mating types resulted in well-developed perithecial fruiting bodies and their crossings were confirmed by the PCR assays for the rapid and specific detection of both MAT1-1 and MAT1-2. This results showed that molecular markers provide an efficient alternative for identification of hybridal status or mating types in the breeding program for artificial cultivation and industrial-scale production of C. militaris.

Cordyceps species are important mushrooms traditionally used for heaths and vitality. C. sinensis has been widely used as a medicinal mushroom and C. militaris is popular for its substitute. C. militaris can be cultivated artificially and new strains has been improved by crossing single spore strains. As a bipolar heterothallic fungus C. militaris has two strains of compatible mating types and they can be differentiated by crossing, fruiting body formation ability and the production of perithecia. However this process is very laborious and time consuming to carry out. In this study, molecular markers were designed and used for the detection of two opposite mating types. Two mating types were assayed using two sets of primers specific for C. militaris, which were amplified a 191-bp fragment for MAT1-2 and 233-bp fragment for MAT1-1. After crossing of two compatible mating types F1 hybrids resulted in well-developed perithecial fruiting bodies and their crossings were confirmed by the multiplex PCR assays for the rapid and specific detection of both MAT1-1 and MAT1-2. This results may serve as a efficient process in the breeding program for artificial cultivation and industrial-scale production of C. militaris.

Mushroom flies are one of the most serious threats to mushroom production and cultivation in Korea, and they cause significant losses in yield and quality. In this study, we investigated the occurrence and control of mushroom flies in farms where Agaricus bisporus was cultivated in Chungnam. The cultivation period was more than 7 years in the 32 mushroom farms examined; about 28% were environment-friendly cultivation farms, and 72% were conventional cultivation farms. Mushroom flies were a major pest in about 84% of the examined farms. Most of the mushroom flies were sciarids and phorids (71% and 24%, respectively). The adult flies were found throughout the year, but their occurrence was the highest in September when the temperature increased. In general, the occurrence of mushroom flies in the examined farms was high; however, insect nets and post-crop steaming programs used as basic control methods were not implemented properly. Our findings show that integrated pest management of mushroom flies is urgently required for A. bisporus cultivation in Korea.

Mushroom flies are the most serious insect pest problems affecting the cultivation of button mushroom, Agaricus bisporus Lange, and among them sciarid flies are the most common. This study was set out to determine the occurrence of mushroom flies at the mushroom farming areas in Chungnam. The occurrence of mushroom flies were highest in September as temperature increases causing yield loss of up to 27%. Adult flies invade mushroom farms and oviposit in freshly spawned compost. They spoil the appearance of the fruiting bodies, spread various fungal and bacterial disease, and transmit mites etc. Damage is mainly caused by the larvae, which feed on growing mycelium and developing stipes and caps of mushrooms. Out of 32 examined farms most of cultivation years were more than 7 years and environmental friendly cultivating farms were about 28% and conventional cultivating farms were 72%. About 84% of the examined farms selected mushroom flies as a major pests. It was found that sciarid were 71%, phorid were 24% as main mushroom flies. The occurrence density of mushroom flies were highest in September as temperature increases. The occurrence of mushroom flies at the examined farms were serious in general. However insect nets and the hygiene program as basic control were not effective. These results offer good potential for control and could implement for the integrated pest management.

Single ascospores were isolated and tested their characteristics such as mycelial growth, mycelial density, fruiting body formation ability, the production of perithecia in the breeding of new Cordyceps militaris mushroom. Among them selected isolates were crossed and hybrids were produced showing high quality fruiting bodies in artificial media. New strain 'Dowonhongcho’ was better on SDAY and at 10~25°C when it was compared with that of 'Yedang 3' in mycelial growth. The stromata of new strain were club-shaped and bright orange-red color. Its length was 6.1 cm and the cordycepin content was 0.34% on average. In comparison with 'Yedang 3', the new strain had a yield that was 9% higher and it produced fruiting bodies which were firmer. The optimum temperature for mycelial growth was 22~25°C and the optimum temperature for stroma development was 18~22°C. Days of fruiting body were similar with 45 days from inoculation. This cultivar may serve as a valuable one for artificial cultivation and industrial-scale production of C. militaris.

Cordyceps militaris is being studied and cultivated as a medicinal mushroom having many valuable biological andpharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and examined theirmycelial growth, mycelial density, and production of stroma and perithecia. Among them selected isolates were crossed andhybrids were produced showing high quality fruiting bodies on artificial media. Mycelial growth rate of new strain‘Dowonhongcho’ was higher than that of better on ‘Yedang 3’ on SDAY at 10-25oC. The stromata of new strain were club-shaped and bright orange-red. Its height was 6.1 cm and the cordycepin content was 0.34% on average. The new strainshowed 9% higher yield than ‘Yedang 3’ with producing firmer fruit bodies. The optimum temperature for mycelial growthwas 22~25oC and the optimum temperature for stroma development was 18~22oC. Fruiting bodies were began to produce45 days later after inoculation. This new cultivar may serve as a valuable one for artificial cultivation and industrial-scaleproduction of C. militaris.

검정날개버섯파리과에 속하는 긴수염버섯파리(Lycoriella ingenua)는 우리나라 양송이버섯 재배에서 심각한 피해를 주는 주요해충의 하나이다. 본 연구는 양송이버섯 재배에서의 긴수염버섯파리의 온도별 발달과 피해양상을 조사하고자 실시하였다. 버섯파리는 균사를 배양한 페트리디쉬에서 사육하였고 16, 20, 24, 28oC 온도에서 알에서 성충까지 소요기간은 각각 35.2, 25.8, 23.5, 22.2일로 조사되었다. 버섯파리 성충은 버섯재배사를 침입하여 종균이 접종된 배지에 알고 낳고 1-7 mm의 부화된 유충에 의해서 균사와 생육하고 있는 버섯의 갓과 대를 섭식하여 가장 큰 피해를 입힌다. 성충은 자실체의 외관을 더럽히고, 여러 가지 곰팡이와 세균병을 전파시키며, 응애 등을 옮긴다. 적절한 해충방제 없는 조건에서 버섯파리에 의한 피해가 증가하면서 자실체 수량은 27%까지 감소하였다.