Since nuclear power plant (NPP) dismantling carries the possibility of radiation exposure from a hazardous environment, it’s important to minimize that by using a remote manipulator et al. However, due to complexity of nuclear facilities, it’s necessary for operators to increase their proficiency by operating in advance in a virtual environment. In this research, we propose a virtual manipulator system using a haptic device for NPP’s reactor vessel internals (RVI) dismantling which can realistically manipulate.

Omni-directional high dynamic range images are used in image-based lighting or environment mapping. In game development environment high dynamic images can be used with shader assets. Recently various 360 VR camera systems are produced which can capture omni-directional images or video images without extra image stitching. In game development environment, shader assets or assets for shaders can be made along very time-consuming complex processes. In this paper, we proposed more simple and autonomous conversion system which can use directly in Unity.

Celiac disease (CD) is classified as an autoimmune disease of small intestine and occurred with people with the human leucocyte antigen (HLA) DQ2(8) cells. The gluten commonly called for the gliadins and glutenins from wheat and related proteins from barley and rye is significant cause of celiac disease. There are many sequences that recognized by T-cell according to species and different types of gliadins. In ω-gliadin, two sort of epitopes were figured out that consisting of some proline(P) and glutamine(Q) scattered in gliadin sequence. All registered ω-gliadin sequences deposited in NCBI database were downloaded and collected. In order to classify groups depending on sequence difference, sequence similarity and their closeness were analyzed by phylogenetic trees using by MEGA (ver.6.06). Chinese spring genome sequence database offered by URGI (Unité de Recherche Génomique Info) is used for sequence assembly. Primers to validate presence of epitopes were designed by two different type from conserved and specific region. Primer pair from consensus region were designed in conserved domain of ω-gliadin sequences from public database by sequence alignment. And, sequence-specific primers of ω-gliadin were designed from the unique region of each ω-gliadin sequence comparing ω-gliadin sequences from NCBI database with draft sequence of Chinese spring in URGI. The two known epitopes of ω-gliadin were located on same site, approximately from the 315th nucleotide to the 348th nucleotide in CDS. Candidate epitopes present in ω-gliadin were divided into three categories based on analysis of sequence similarity. This categorization shows similar pattern with groups that were previously reported by sequence motifs such as SRLL, AREL, ARQL and KELQ. However, sequence which has AREL motif and sequence ARQL motif were not distinguished obviously in ω-gliadin based on sequence alignment.

Wheat is the third largest crop in the world behind corn and rice. Wheat is grown over a wide range of environments, and an essential source of carbohydrates. However, the genomics of wheat, a non-model species, is still challenging despite of corn and rice was done. The recent advent of RNA-Sequencing, a massively parallel sequencing method for genome and transcriptome analysis, provides opportunity to identify gene discovery and molecular mechanisms of cellular processes. We performed a RNA-Seq experiment to find differentially expressed genes under high temperature condition. More than 344 million shot reads were generated using Illumina HiSeq technology. A comprehensive and integrated 285,324 transcripts were assembled via Trinity by combining tentative consensus sequences. Transcripts annotated by BLAST2Go and differently expressed transcripts were analyzed. A total of 208 up-regulated and 182 down-regulated transcripts were found that involve in plastid, starch and sucrose metabolism, glycerolipid metabolism and glycerolipid and dicarboxylate metabolism. Our results demonstrate that RNA-Seq can be successfully used for gene identification, transcript profiling in wheat. Furthermore these sequences will provide valuable resources for wheat researchers.

The main nutritional problem of sorghumxsudangrass hybrid (Sorghum bicolorxSorghum sudanese) silage is low quality and palatability. This experiment was conducted to evaluate whether organic com grain and crushed rice addition of sorghumxsudangrass hybrid silage increases forage quality of the silage. The sorghumxsudangrass hybrid silages with added com grain and crushed rice were similar to conventional corn silage in moisture content. However organic sorghumxsudangrass hybrid silage had low pH values. Silage added with gain and byproduct had higher crude ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF) contents than control corn silages, while its non-fiber carbohydrate (NFC) and total digestible nutrients (TDN) showed the opposite results. Lactic acid, butyric acid and lactic percentage oftotal organic acid (UT) of sorghumxsudangrass hybrid silages were higher than those of com silage, but acetic acid was higher than control. In vitro dry matter digestibi/ity (IVDMD) of com silage was higher than other sorghum silages. Feed intake of sorghumxsudangrass hybrid silage added with crushed rice was highest among silages. Therefore, these data indicate that crushed rice and com grain added sorghumxsudangrass hybrid silage could be recommended as the most effective treatment for increasing silage quality and palatability.

For sensitive and accurate gene expression analysis, normalization of gene expression data against housekeeping genes is required. There are conventional housekeeping gene (e.g. ACT) that primarily function as an internal control of transcription. In this study, we performed an in silico analysis of 278 rice gene expression samples (GSM) in order to identify the gene that is most consistently expressed. Based on this analysis, we identified novel candidate housekeeping genes that displayed improved stability among the cross experimental conditions. Furthermore four of the most conventional housekeeping genes were included in our 30 other housekeeping genes among the most stable genes. Therefore, these 30 genes can he used to normalize transcription results in gene expression studies on rice at a broad range of experimental conditions.

MYB proteins are a superfamily of transcription factors (TF) that play regulatory roles in developmental processes and resistance mechanism in plants. We identified 130 and 109 genes in the MYB superfamily from an analysis of the complete Arabidopsis and rice genome sequence. Although microarray based transcriptome analysis approach allows the investigation of the biological networks of MYB TF in DNA level, the underling mechanisms related to their functional role is not fully understood. In this work, we performed meta-analysis of public microarray data that analyzed with Arabidopsis and rice using co-expression analysis. A phylogenetic comparison of the members of this superfamily were performed with Sorghum bicolour to suggested that MYB super family underwent a rapid expansion their evolutionary times. We identified conserved expression pairs which play important role in transcription. Our comprehensive analysis of this huge transcription factor of Arabidopsis and rice may shed further light on the possible biological roles of the MYB TF in various plants.

Rapid extension of genomic database leads to the remarkable advance of functional genomics. This study proposes a novel methodology of functional analysis using 5-methyltrytophan (5 MT) mutant together with their 2-DE analysis and public microarray database. A total of 24 proteins was changed in 5 MT mutant and four remarkably different expressed proteins were identified. Among them, three spots were converted to Affymetrix probe. A total of 155 microarray samples from Gene Expression Omnibus (GEO) in NCBI was retrieved and followed by constructing gene co-expression networks over a broad range of biological issues through Self-Organising Tree Algorithm. Three co-expressing gene clusters were retrieved and each functional categorization with differential expression pattern was exhibited from 5 MT resistance mutant rice. It was indicated new co-expression networks in the mutant. This study suggests that on investigating possibility which correspond 2-DE to microarray database with their full potential.

Most gene functions of biochemical pathways were still unexplored, especially interactions of constituent genes. We attempted to uncover interaction network of biochemical pathways via a survey of co-expression clusters, which we have constructed from the NCBI GEO database, and then to define key genes of networks with expression correlations between members. Top 20 pathways with high numbers of individual genes were retrieved from 178 pathways. One pathway, ‘removal of superoxide radicals’ was excluded for further study, evidencing somewhat low degree (16%, 13 out of 79 genes) of mapped probes. We employed expression correlations of random pairs of 1,000 randomly selected genes for determining a cut off r-value for gene networks. Numbers of interactions with a significant expression correlation values between members might evidence that “hub genes” play key roles among a given pathway genes. For example most interactive pathway, ‘tRNA charging pathway’, that is composed of 60 probes corresponding to genes showed 264 positive significant interactions between members of 47 genes while 5 negative interactions between members of 7 genes., evidencing ‘Os10g26050’ (methionyl-tRNA synthetase) gene with highest interactions is suggestive of a hub gene. These findings might provide some clues on evolutionary fate of co-expression genes including each of biochemical pathways, e.g. convergent evolution

The genetic diversity was evaluated using RAPD and ISSR among natural populations and Korean wheat cultivars (Triticum aestivum). Understanding the genetic diversity of putative parental and wild stocks would be useful in wheat breeding programs. Ninety three populations were evaluated with fifty RAPD and three ISSR primers. A total of 185 RAPD and ISSR polymorphism were produced. These markers were considered to estimate the genetic distance among accessions. The genetic similarity ranged from 0.41 to 0.86. The dendrogram were constructed by using the UPGMA clustering algorithm based on genetic similarity. The genetic diversity within and among accession was assessed through Principal Component Analysis (PCA) for statistics analysis. In cluster analysis, four groups were clustered and 17 accessions were not clustered. The PCA was corresponded well to the result. This study provides basic information about the genetic relationships for breeding purposes.