Soybean proteins are widely used for human and animal feeds worldwide. The use of soybean protein has been expanded in the food industry due to their excellent nutritional benefits. But, antinutritional and allergenic factors are present in the raw mature soybean. P34 protein, referred as Gly m Bd 30K, has been identified as a predominant immunodominant allergen. The objective of this research is to identify the genetic mode of P34 protein for the improvement of soybean cultivar with a very low level of P34 protein. Two F2 populations were developed from the cross of "Pungsannamulkong" x PI567476 and "Gaechuck2ho" x PI567476 (very low level of P34 protein). Relative amount of P34 protein was observed by Western blot analysis. The observed data for the progeny of "Pungsannamulkong" and PI567476 were 133 seeds with normal content of P34 protein and 35 seeds with very low level of P34 protein (X2=1.157, P=0.20-0.30). For the progeny of "Gaechuck#1" and PI567476, the observed data were 177 seeds with normal content of P34 protein and 73 seeds with very low level of P34 protein (X2=2.353, P=0.10-0.20). From pooled data, observed data were 310 seeds with normal content of P34 protein and 108 seeds with very low level of P34 protein (X2=0.156, P=0.50-0.70). The segregation ratio (3:1) and the Chi-square value obtained from the two populations suggested that P34 protein in mature soybean seed is controlled by a single major gene. Single gene inheritance of P34 protein was confirmed in 32 F2 derived lines in F3 seeds, which were germinated from the low level of P34 protein obtained from the cross of "Pungsannamulkong" and PI567476. These results may provide valuable information to breed for new soybean line with low level of P34 protein and identification of molecular markers linked to P34 locus.

Italian ryegrass (Lolium multiflorum Lam.) is one of important forage crop grass widely cultivated in Korea. The genetic manipulation of Italian ryegrass (Lolium multiflorum Lam.) necessitates a reliable and efficient, genotype-independent method of transformation. We are interested in developing molecular breeding methods to improve its nutritional quality and abiotic stress resistance. Development of a rapid and efficient transformation system is the basis for genetic manipulation of Italian ryegrass. In order to establish an efficient Agrobacterium-mediated transformation system was applied to transfer genes into seven genotypes of Italian ryegress, namely cv. 'Kogreen', 'Kopeed', 'Kowinearly', 'Kowinmaster', 'Hwasan 101', 'Hwasan104' and 'Kowinner.' The transformation system developed in this study would be useful for Agrobacterium-mediated genetic transformation of Italian ryegrass plants with genes of agronomic importance.

Silage corn can make good livestock feed. Anytime drought occurs during the growing season, corn grain yields will be affected. The extent of the loss will depend on stage of grow and the length of time the crop is subject to droughty conditions. During times of extended drought when grain yield potential is sever limited or nonexistent, the plants may still offer a valuable source of nutrients for livestock provided careful attention is given to how it is harvested and fed. As a rule, drought-damaged corn will have 85 to 95 percent of the feeding value of normal corn silage. Ideally, corn silage would be 60 to 70 percent moisture at harvest. Dry conditions around the state cause many corn producers to wonder about making silage from drought-damaged corn. Although silage made from drought-damaged corn is usually not as good as that made from unstress corn, drought -damaged corn can make good livestock feed. This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field of Chonnam Province. As a result of experiment, production of silage corn reduced more than 60% compared to that of normal condition.

Italian Ryegrass, also called annual ryegrass, is a high productivity and feed value, and an upright grass that behaves like a biennial or short-lived perennial. It grows vigorously in winter and early spring. Italian ryegrass and a related species, perennial ryegrass, Lolium perenne, are the two common weedy ryegrassses. Italian ryegrass and perennial ryegrass can hybridize, resulting in offspring that are difficult to identify as either species. Ryegrasses are cultivated for turf and forage. Sometimes Italian ryegrass is grown as cover crop. It has the potential to produce high yields and, with proper management, can be high quality with good animal performance. To develop of a high quality, productivity and early variety, 11 varieties were in the seven different irradiation conditions (0Gy, 100Gy, 200Gy, 300Gy, 400Gy, 500Gy, 1,000Gy) and examined growth and germination characters of each plant as like plant height, number of tillers. As a increase of irradiation, germination rate of 11 Italian ryegrass varieties are somewhat reduced.

dlm allele controlling disease lesion mimic trait is useful in basic research aimed at better understanding disease hypersensitive response and programmed cell death in soybean. Inheritance between dlm trait and any morphological trait, position of dlm allele on classical linkage group, molecular marker linked to dlm allele were not reported. Two populations [T255 (lf2lf2DlmDlm) x T363 (Lf2Lf2dlmdlm), T363 (dlmdlmp1p1) x T43 (DlmDlmP1P1)] were made to find independent assortment or linkage between dlm locus and lf2 or between dlm locus and P1 locus. The segregation ratios of 3 : 1 were observed in the F2 population and the Chi-square values strongly suggested that the disease lesion mimic and seven-leaflet trait was controlled by a single recessive gene. Glabrousness trait was controlled by a single dominant gene. Segregation ratios of 48 Lf2_Dlm_: 30 Lf2_dlmdlm: 21 lf2f2Dlm_ : 8 lf2lf2dlmdlm based on F2 phenotype showed that dlm allele was inherited independently with the lf2 allele controlling seven-leaflet trait in soybean. However, more F2 plants will be needed to confirm this result. Also, segregation ratios of 137 P1_Dlm_: 46 P1_dlmdlm: 49 p1p1Dlm_ : 16 p1p1dlmdlm from F2 phenotype confirmed strongly that dlm allele was inherited independently with the P1 allele controlling glabrous trait in soybean. This results indicate that dlm allele will not located in soybean classical linkage group 2 (molecular linkage group K) and soybean classical linkage group 16. This observation will helpful to attempt to position the Dlm locus on the soybean molecular linkage map.