Cell transplantation therapy using adult stem cells has recently been identified as a potential treatment for spinal cord injury (SCI). But, recovery after traumatic SCI is very limited. As dogs are physiologically much more similar to human compared with other traditional mammalian models in disease presentation and clinical responses, a number of researches demonstrated canis familiaris is a suitable model for human diseases. This study investigated the effect of transplantation of canine Mesenchymal Stem Cells (cMSC) and neural-induced cMSC (nMSC) to understand how these cells improve neurological function in canine SCI model. The differentiation of cMSC into neural precursor cells was induced in dulbecco’s modified eagle’s medium supplemented with N2-supplement, dibutyryl cyclic adenosine monophosphate, and butylated hydroxyanisole. SCI was induced between T1 and T2 by surgical hemi-section in adult dogs, and then assigned to two groups according to the applied cell types (cMSC vs nMSC). Pelleted cMSC or nMSC were transplanted directly into the injured site after SCI, respectively. Analysis of motor function after transplantation was evaluated by modified Olby score. Magnetic resonance imaging (MRI), histological and immunohistichemical analysis were also performed. Functional recovery in group of cMSC was increasing gradually after transplantation and was higher than nMSC. In MRI, we could not confirm any difference between the cMSC and nMSC experimental groups. Immunohistochemically, beta3-tubuline and nestin were observed in injury site of two experimental groups with the expression level close to non-injured groups. Transplantation of mesenchymal stem cells could promote neuronal reconstruction and repair motor function in SCI. These showed mesenchymal stem cells could be a great candidate as a therapeutic tools in degeneration disease, and dogs could be used to explore human regenerative medicine as a promising animal model. This research was supported by iPET (Grants 110056032CG000), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

Acteoside (verbascoside) is a typical phenylethanoid glycoside, extracted from various plants. It has various biological functions such as anti-oxidant, anti-inflammation, and anti-hypertension. Specially, it was powerful anti-oxidants either by direct scavenging of reactive oxygen and nitrogen species, or by acting as chain-breaking peroxyl radical scavengers. We examined the role of acteoside in IVM medium on the morphological progress of meiosis, developmental competence, and ROS in porcine oocytes. And we investigated effect of acteoside on the oocytes condition represented by cytoplasmic maturation by homogeneous distribution and formation of cytoplasmic organelles and regulation of apoptosis-related genes. The selected COCs were cultured in TCM-199 with various concentration of acteoside: 0 (control), 10, 30, and 50 μM. After 22 h of maturation with hormones, the oocytes were washed twice in a fresh maturation medium before being cultured in hormone-free medium for additional 22 h. The oocytes maturation rates of supplemented with acteoside were no significantly different compared with control group (71.13, 75.96, 72.95 and 73.68%, respectively). Level of ROS was significantly decreased in acteoside treated group. Furthermore, the parthenogenetic blastocyst rate was significantly improved in 10 μM acteoside treated group compared with control group (40.03 vs. 22.95%). During IVM, 10 μM acteoside treated oocytes showed that the mitochondria and lipid droplet were smaller and homogeneous distribution in cytoplasm compare with non-treated control oocytes. And reverse transcription polymerase chain reaction (RT-PCR) witarthenogenetic blstocysts revealed that acteoside increased the anti-apoptoticgenes, otherwise reibued pro-apoptotic genes. In conclusion, our results represents that addition of acteoside to the IVM medium has a beneficial effect in physiology of porcine oocytes such as viability and activation, providing a improved method for porcine oocytes in vitro.

도암만은 전남 장흥군, 강진군, 해남군, 완도군 등 4개 군으로 둘러싸인 전형적인 하구형 만으로, 남북방 향 길이 약 19km, 최대너비 약 7km의 네 방향으로 갈라진 십자형의 형태로, 만내에는 크고 작은 8개의 유 인도 및 무인도가 있으며, 남부에는 완도, 고금도 등이 위치하여 이들 사이의 수로를 통해 해수가 제한적으 로 유출입하는 반폐쇄성 만으로 파랑에 의한 영향이 적다. 만내로 전란남도의 3대강 중의 하나인 탐진강 하천수와 강진천, 칠량천, 대구천의 하천수가 유역으로 유입되면서 담수와 해수의 교환이 이루어지고 담수 유입량에 의해 조류가 영향을 받고 있다. 효율적인 내만의 수질관리를 위해서는 우선 부하발생원에 따른 오염배출량이 어느 정도 인지 확인하고 해역의 환경용량과 환경기준을 만족하기 위해서 부하삭감량을 산정하는 사전대책이 요구된다. 연안해역의 수질에 영향을 끼치는 부하발생원은 크게 점오염원과 비점오염원으로 구분되는데, 비점오염원은 강우시 도 시지역이나 농경지 및 산림지역의 유출수로 파악될 수 있지만, 유입지점을 명확하게 찾기 어렵고, 일간 계 절간 배출량 변화가 크며 예측과 정량화가 어려워서 관리가 힘든 특징이 있다. 이에 반해 점오염원은 가정 하수, 공장폐수 등으로 구성되며 일정한 지점에서 일정량이 지속적으로 발생하여서 배출구와 배출단위가 파 악가능하기 때문에 수질관리를 위한 부하삭감량 산정의 주 대상이 되고 있다. 따라서 본 연구에서는 해역으로 유입하는 외부부하량을 실측하고 분석하여 득량만의 효과적인 연안해역 의 수질관리의 기초자료로 활용하고자 한다. 하천 부하량 조사는 도암만 유역에 포함되는 하천 24개 지점을 대상으로 하였으며, 조사 시기는 3월부터 12월까지 매월 1회 실시 하였고, 각각의 유입하천은 직독식 전자 유속계(ACM-200PC) 및 부표를 이용하여 유속을 측정한 다음, 단면적을 계산하여 각 유입원의 유량을 산정 하였다. 각 시점에서 채수한 하천수의 pH, 수온, 염분, 전기전도도, 화학적 산소요구량(CODMn) ), 총질소(T-N), 총인(T-P), 용존산소(DO), 부유물 질(SS), 용존무기질소(DIN), 용존무기인(DIP) 등을 분석하였고, 환경부 수질오염공정시험방법(환경부, 2008), 해양환경공정시험기준(국토해양부, 2008), 준하여 분석하여 유량과 곱해서 그 부하량을 산정하였다. 하천 유량의 월별 총합은 821590∼5971583 m3/day 이고, 부유물질(SS)과 화학적 산소요구량(COD), 용존 무기질소(DIN), 용존 무기인(DIP), 총질소(TN), 총인(TP), 월별 부하량은 각각 6070.3∼47781.3(평균 20999.4)kg/day, 3570.5∼21471.5(평균 9455.2)kg/day, 843∼5042.4(평균 2015.1)kg/day, 14.2∼163.7(평균 66.7)kg/day, 1249.2∼10166.9(평균 4151.3)kg/day, 47.3∼390.8(평균 165.9)kg/day 이다.