Cloned calves derived from somatic cell nuclear transfer (SCNT) have been frequently lost by sudden death at 1 to 3 month following healthy birth. To address whether placental anomalies are responsible for the sudden death of cloned calves, we compared protein patterns of 2 placentae derived from SCNT of Korean Native calves died suddenly at two months after birth and those of 2 normal placentae obtained from AI fetuses. Placental proteins were separated using 2-Dimensional gel electrophoresis. Approximately 800 spots were detected in placental 2-D gel stained with coomassie-blue. Then, image analysis of Malanie III (Swiss Institute for Bioinformatics) was performed to detect variations in protein spots between normal and SCNT placentae. In the comparison of normal and SCNT samples, 8 spots were identified to be up-regulated proteins and 24 spots to be down-regulated proteins in SCNT placentae, among which proteins were high mobility group protein HMG1, apolipoprotein A-1 precursor, bactenecin 1, tropomyosin beta chain, H+-transporting ATPase, carbonic anhydrase II, peroxiredoxin 2, tyrosine-rich acidic matrix protein, serum albumin precursor and cathepsin D. These results suggested that the sudden death of cloned calves might be related to abnormal protein expression in placenta.

본 시험은 우리나라에서 발생하는 푸른곰팡이병균의 종의 빈도와 발병환경 및 방제법을 구명코져 실시하였다. 시험결과 Trichoderma koningi, T. lignorum, T. glaucum과 미동정의 1종등 4종의 병원균이 분리되었고 이들의 빈도는 각각 와 이었다. 푸른곰팡이병균은 감자배양액, 왁스만배양액과 리차드배양액에서 생육이 잘 되었으며 중성-염기성배지에서는 생육이 불량한 반면 산성에서 생육이 왕성하였으며 최적산도는 pH4였다. 양송이 수확기간 중 재배사내의 온도는 내외 일 때 본명의 발생이 적었고 수량이 많으며 이상에서는 본병의 발생이 격심하였다. 푸른곰팡이병균은 복토흙 소독시 에서 60분, 혹은 에서 30분간 열처리하므로서 완전히 사멸하였고 퇴비 후발효 과정에서도 사멸되었다.

치과 콘빔 전산화단층 검사 시 갑상선 부위의 방사선 피폭선량을 최소화하기 위하여 보루스 (Bolus)를 이용한 차폐체를 제작하고, 방사선 차폐 효과와 영상의 적정성을 평가하였다. 치과용 콘빔 전산화단층검사 장비를 사용하여 치과방사선 두부 팬텀을 대상으로 갑상선의 좌, 우측 부위에 유리선량계를 부착시켜 방사선량을 측정하였다. 차폐체의 두께를 각각 10 mm, 20 mm, 30 mm로 다르게 하여 각 차폐체별로 흡수선량을 측정하여 차폐체를 사용하지 않았을 경우와 비교하였다. 8명의 평가자가 진료영상의 적정성을 여부를 평가하였다. 왼쪽 갑상선 부위에 30 mm Bolus 차폐체를 사용한 경우에는 결과값이 평균 342.67 μ㏉로 Bolu s 차폐체를 사용하지 않고 측정한 결과값의 평균 431.22 μ㏉보다 20.7% 감소하였고, 오른쪽 갑상선 부위에 30 mm Bolus 차폐체를 사용한 경우에는 평균 424.56 μ㏉로 21.9%의 선량감소효과를 보였다. 진료 영상의 적정성은 평가자 모두 사용 가능하다 판단하였다. 결론적으로, 치과 콘빔 전산화단층검사 시 갑상선 부위의 방사선 피폭선량을 최소화하기 위해 제작된 Bolus 차폐체는 장해음영 없이 적정한 진단 영상 처리가 가능하고, 방사선차폐 효과가 있어 유용한 차폐체로 적용 가능할 것으로 사료된다.

약물용출 스텐트(drug-eluting stents, DES)는 일반 금속 스텐트에 비하여 재협착을 현저하게 줄였지만, 여전히 관상동맥 스텐트 재협착은 비율이 높다. 2012년 11월부터 2016년 12월까지의 일 대학교병원 심혈관센터에서 경피적 관상동맥 스텐트 삽입술 후 관상동맥 조영술에서 스텐트 재협착 환자 187명 그룹 I (약물코 팅 풍선카테터 사용, n=127명), 그룹 II (약물용출 스텐트 사용, n=60명)로 분류하여 치료효과, 주요심장사건, 사망 발생률, 심근경색, 표적병변 재개통술 그리고 스텐트 혈전등을 2년 동안 추적 분석하였다. 임상적 특성는 두 그룹간 차이는 없었고(21.1 ± 5.3 vs. 25.3 ± 9.6 mm, p<0.002), 혈관조영검사에서 약물코팅 풍선 카테터 사용 그룹에서 스텐트 재협착 길이가 짧았다. 주요심장사건은 8.7%vs.10.0%, p=0.789, 사망발생률 0%vs.0%,p=1.000, 심근경색 1.6%vs.6.7%, p=0.085, 표적병변 재개통술 8.7% vs. 10.0%, p=0.789 그리고 스텐트 혈전증 0% vs. 0%, p=1000에서 양군 간에 차이를 보이지 않았다. 약물코팅 풍선카테터가 약물방출 스텐트와 비교하여 2년 추적 검사 결과 주요심장사건에서 차이가 없었고, 약물코팅 풍선카테터는 스텐트 재협착 병변에서 약물방출 스텐트와 함께 선택할 수 있는 좋은 치료방법이라고 사료된다.

본 연구는 일반인을 대상으로 시대성이 반영된 각종 잠재적 위험요소와 원자력(방사선)발전의 위험성 대 한 인식을 분석하였다. 설문 대상은 다양한 계층으로 하고 그 중에 총 293부를 분석하였다. 그 결과, 잠재 적 위험요소 중에서 화재에 대한 위험도 인식이 높게 나타났으며, 다음으로 방사선 테러와 핵(원자력)에너 지의 위험도를 다른 위험요소들에 비해 비교적 높게 인식하였다. 연령별, 학력별, 정치 이념 성향에 따른 분석에서 원자력발전의 필요성과 위험성, 안전성에 대해서 상반된 결과를 나타내었다. 정치이념의 성향에 따른 잠재적 위험요소와 원자력발전에 대한 인식은 보수적 이념집단에서 긍정적인 인식을, 진보적 집단에 서는 부정적인 인식을 하고 있는 것으로 나타났다. 즉, 정치 이념의 성향에 따라 원자력발전 인식 분석에서 통계적 차이를 보였다. 따라서 원자력(방사선)발전 정책 방향 설정과 방사선 이용 관련 산업에 있어서 전문 가 의견과 일반인의 다양한 의견을 반영해서 결정되어져야 할 것으로 판단되고, 일반인도 사실을 바탕으로 객관적이고 과학적인 근거에 입각해 각종 잠재적 위험과 원자력(방사선)에 대해 막연한 불안감을 가지지 말고 유연한 대처를 할 필요가 있다고 판단된다.

This study was conducted with the aim of evaluating the antibiotic effects of leaves, stems, and roots in yacon (Smallanthus sonchifolius). Antibacterial activity of leaf extract by disk diffusion method with Bacillus subtilis and Escherichia coli respectively showed 13.3 and 13.75mm diameters of clear zone. There was no significant difference between the stems and leaves. The minimum inhibitory concentration of leaves’ heating and agitation extraction showed a restrain of strain at 1mg/ml, but the stems and root extract did not appear. Yacon is a functional antibacterial material, and methanol extraction is more effective than water. This research was to investigate the growth stage of collection has the most effective antibacterial effects. It has collected yacon's leaves from June to October, which is an appropriate time for collection right before reaping. Yacon leaf has antibacterial effects on Bacillus subtilis, Escherichia coli, Enterococcus faecium, and Salmonella enteritifis. There were no significant differences by the growth stage of collection. Leaves collected in July are high in phenol which helps in sulfating activity works well considering the high scavenging capability of DPPH. Leaves collected in September are high in total flavonoid.

Background : Recently, ginseng (Panax ginseng C.A. Meyer.) berry has been used as a health-promoting supplements. Also, Mulberries (Morus alba L.) fruit have been used in traditional herbal medicine to treat and prevent diabetes. In this study, we measured the cytotoxicity after fermentation of the extracts in Panax Ginseng Berry and Mulberry Fruit. Methods and Results : The extracts were prepared by decoction for 3 hours in distilled water (100 g/L). The dried extract was then dissolved in phosphate-buffered saline (PBS) in preparation for use. Cell viability was examined by an MTT assay. RAW 264.7 cells were seeded at 1 × 104/mL densities in 96-well plates. Each grouping had a non-treated group as the control. The extracts were added to each well and incubated for 24 hours at 37°C and 5% CO2. The MTT solutions (5 mg/mL) were added to each well, and the cells were cultured for another 2 hours. The supernatant was then discarded, and 100 μL of dimethyl sulfoxide was added to each well. The optical density was read at 540 nm. Conclusion : Probiotics and prebiotics modulate the composition of human and domestic animal gut microbiota. The beneficial effects may result from suppression of harmful microorganisms or stimulation of organisms which contribute in a positive way to the nutrition and health of human and domestic animal. Recently, fermentation using microorganisms for the production of more effective compounds has been extensively studied. In particular, the novel pharmacological effects of a new compound generated by fermentation have been reported. Some previous studies have demonstrated that Fermented herbal medicine extract showed better bioactivity than normal herbal Plants extract when used at the same concentration.

Background : According to medicinal plant standard culture, flower organ should be removed but there is no detail information on flower organ removal in Atractylodes macrocephala Koidzumi. This study was carried out to examine effect of flower organ removal on the increase of roots yield in Atractylodes macrocephala Koidzumi. Methods and Results : ○ Experiment variety : Atractylodes macrocephala Koidzumi. ○ Treatment : ① Flower organ non-cutting ② Flower organ cutting ○ Planting date : April, 2015/ April 25, 2016 ○ Planting distance : 30×20cm/ 30×15cm ○ Experiment place : Ginseng & Medicinal Plant Research Institute(Geumsan-gun, Chungcheongnam-do) Conclusion : The fresh rhizome yield of F. O. C. at a bud treatments were increased 21.0% ∼50.0% compare to F. O. N. C. treatments. The fresh rhizome yield of F. O. C. at a flower treatments were increased 17.4%∼34.6% compare to F. O. N. C. treatments. The fresh weight of above-ground parts of F. O. C. treatments were decreased 77.2%∼65.7% compare to F. O. N. C. treatments. F. O. C.(=Flower organ cutting) / F. O. N. C.(=Flower organ non-cutting).

Background : This study was carried out to understand the effect of seedling weight (SW) on growth and flowering in Panax ginseng. Methods and Results : The testing materials were Chunpoong (CP), Yunpoong (YP) and Jakyeongjong (JK). The increase of seedling (1yr) weight led to an increase in ratio of flowering plant and in number of flower per plant. The seed setting rate of two year-old plant (CP, YP, JK) increased with increase of SW at the planting time (PT) and number of flower per plant of three year-old plant (CP, YP) increased also. In the two year-old plant (JK), the ratio of three leaves per plant was 8.8, 19.6, 31.0, 42.0, 44.7 and 58.2%, respectively, in the SW of >0.6, 0.6~0.8, 0.8~1.0, 1.02~1.2, 1.2~1.4 and 1.4g<. The growth of ginseng plant was good with increase of SW at the PT. Conclusion : There was a highly positive correlation between seedling weight and flowering characteristics.

Background : Jiwhang (Rehmannia glutinosa L.) cultivation area of chungnam province was 62ha(219 farms, 53.9% of domestic cultivation area, M.A.F.R.A. 20142)). Recently, jiwhang is rapidly spread to 150ha ( Geumsan administration statistics, 2015) as taking place of income crop in Chungnam. High quality jiwhang GAP production and improvement of the distribution system including magnus customers, producer association fosterage and conventional market Vitalization were the reason of rapid cultivation area increase of Rehmannia glutinosa L. in Geumsan. This study was performed to set up the standard Rhizome size for planting in Rehmannia glutinosa L. Materials and Methods ○ Experiment variety : Togang ○ Treatment(rhizome length) : ① 1cm ② 2cm ③ 3cm ④ 4cm ⑤ 6cm(control) ○ Planting date : May 6, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : In the aboveground growth was no significant difference among treatments. In the root characteristics, there was no significant difference among treatments. Take a 1∼2cm rhizome planting saved rhizome amount and planting time to 73.8%∼64.3% and 68.4%∼ 57.8% respectively than 6cm(control).

Background : Generally, price of Jiwhang(Rehmannia glutinosa L.) is highest in August. In this study, Economic Evaluation and Productivity of 9 Jiwhang varieties w ere analyzed according to harvesting tim es of August and October. Materials and Methods ○ Experiment variety : Jiwhang 1, Korea jiwhang, Daegyung, Gogang, Togang, Dagang, Wongang, Hwanggang and Yeongang ○ Treatment : ①Initial-August harvest ②Mid-August harvest ③Ended-August harvest ④Ended-October harvest ○ Planting date : April 17, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : The higher roots yield at August harvest was in Gogang, Dagang and Togang. The lower roots yield at August harvest was in Korea jiwhang and Hwanggang. The higher economic feasibility at August harvest was in Gogang, Togang, Dagang and Wongang. The higher economic feasibility at ended-October harvest was in Gogang, Yeongang, Togang, Dagang and Wongang.

Background : According to medicinal plant standard culture, flower stalk should be removed at the stage of 4-5 main leaves appearance but there is no detail information on stalk removal in jiwhang (Rehmannia glutinosa L.). This study was carried out to examine effect of flower stalk removal on the increase of total yield in Rehmannia glutinosa L. Materials and Methods ○ Experiment variety : Korea jiwhang, Wongang ○ Treatment : ① Peduncle non-cutting ② Peduncle cutting ○ Planting date : April 18, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : Aerial part growth was no significant difference according to treatments in Wongang and aerial weight was havier in Peduncle cutting than in Peduncle non-cutting. Aerial part growth was no significant difference according to treatments in Korea jiwhang. The roots yield in Korea jiwhang was increased about 5.2% in Peduncle cutting. The roots yield in Wongang was increased about 24.9% in Peduncle cutting.

Background : Bai Zhu is generally harvested in 1-2 years after seedstock planting. Recently mainly in Guemsan, Bai Zhu is harvested in just 1 year after planting in spring . According to medicinal plant standard culture, Bai Zhu should be directly sown by 20cm distance in April. This study was carried out to find out proper planting date. Materials and Methods ○ Experiment variety : Bai Zhu (Atractylodes macrocephala Koidz.) ○ Treatment : Planting Date ① Late march ② Early april ③ Middle of april(control) ④ Late april Plantind density : ① 30×10cm ② ① 30×20cm ○ Planting type : Direct Seeding ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : Number of lateral branches in 30×10cm was more in late march than late April and aerial part growth was superior as planting date was earlier. Emergence rate was higher in middle of April and late April and aerial part weight, fresh root weight, dry root weight rate, and dry root weight were superior as planting date was earlier. Dry weight yield increased as 38.3% in late March compared to middle of April. Dry weight was havier as 36.2% in planting time of late March and early April than planting time of middle of April and late April. Total income was higher in planting time of late March and early April than planting time of middle of April and late April.

Background : Jiwhang (Rehmannia glutinosa L.) cultivation area of Chungnam province was 62ha (219 farms, 53.9% of domestic cultivation area, MAFRA. 20142)**). Recently, jiwhang is rapidly spread to 150ha (Geumsan administration statistics, 2015) as taking place of income crop in Chungnam. High quality jiwhang GAP production and improvement of the distribution system including magnus customers, producer association fosterage and conventional market vitalization were the reason of rapid cultivation area increase of Jiwhang in Geumsan. But low emergence rate caused by spring drought should be solved. This study was carried out to obtain basic data for emergence rate improvement in jiwhang. Materials and Methods ○ Experiment variety : Korea jiwhang ○ Treatment : ①Control ② 1 time irrigation at planting time ③1 time irrigation at planting time + transparent vinyl mulching ○ Planting date : April 30, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : There was no significant difference in the aboveground growth among treatments. The seedling emergence rate was higher in 1 time irrigation at planting time + transparent vinyl mulching(1ITVM) than control or 1 time irrigation at planting time(1I). Aerial part weight was significantly havier in 11ITVM than control or 1I. The roots yield was also more in 1ITVM than control or 1I. According to economic analysis, total income in 1ITVM compared to 1I and control was 125.1% and 54.0% higher respectively.