The mu opioid receptor (MOR) has been regarded as the main site of interaction with analgesics in major clinical use, particularly morphine. The repressor element-1 silencing transcription factor (REST) functions as a transcriptional repressor of neuronal genes in non-neuronal cells. However, it is expressed in certain mature neurons, suggesting that it may have complex and novel roles. In addition, the interactions between MOR and REST and their functions remain unclear. In this study, we examined the effects of morphine on the expression of REST mRNA and protein in human neuroblastoma NMB cells to investigate the roles of REST induced by MOR activation in neuronal cells. To determine the effects of morphine on REST expression, we performed RT-PCR, real-time quantitative RT-PCR, western blot analysis and radioligand binding assays in NMB cells. By RTPCR and real-time quantitative RT-PCR, the expression of REST was found to be unchanged by either the MOR agonist morphine or the MOR specific antagonist CTOP. By western blot, morphine was shown to significantly inhibit the expression of REST, but this suppression was completely blocked by treatment with CTOP. In the radioligand binding assay, the overexpression of REST led to an increased opioid ligand binding activity of endogenous MOR in the NMB cells. These results together suggest that morphine inhibits the expression of REST in human neuroblastoma cells through a post-transcriptional regulatory mechanism mediated through MOR.

Coupled with the international expansion of Korean culture in recent years, a number of restaurants from Korea have been trying to tap into the global market place. The purpose of this study was to identify the characteristics of non-Korean patrons in Korean Restaurants in New-york city. The survey was conducted at six popular Korean restaurants, all of which had been recognized in the Zagat Survey in recent years, located in prime business districts in Manhattan. The data collected from the six local Korean restaurants that participated in this study were qualitatively and quantitatively analyzed. After employing individual in-depth interviews with restaurant operators, a qualitative analysis identified demographic characteristics, Socioeconomic characteristics and segmentation of restaurant operation. Self-administrated survey questionnaires were used to acquire quantitative data. Primary data were collected from non-Korean patrons at the six participating Korean restaurants in New York City in 2008 (N=245). The patrons who answered the survey indicated that they were highly satisfied with the ‘Food’; however, they were not satisfied with the ‘Beverage’ and ‘Value’. In addition, older patrons (55<) were not as content with the ‘Food’ as the younger patrons. The most influential satisfaction variable that affected a patron’s intention to revisit the Korean restaurant was ‘Food’ and ‘Overall experience’. This study findings will help Korean restaurant operators and marketers better understand their patrons and formulate strategies to cater and target segments more effectively.

Lesch-Nyhan 증후근(LNS)은 hypoxanthine guanine phosphoribosyle transferase(HGPRT) 효소를 암호화 하는 X 염색체가 불완전해서 일어나는 유전적인 추제외로계(또는 기저핵)의 드문 병변이다. 출생시 LNS 유아는 정상적인 운동발달이 관찰되어진다. LNS에게서 현저하게 진단적인 특징으로 보여지는 운동심리적 행동인 self-mutilating 행위는 4살 이후에나 나타난다. LNS 아이들은 오히려 초기에

Problem of the diffuse radiation (DFR) transfer is solved exactly for pure hydrogen nebulae of uniform density, and accuracies of the on-the-spot (OTS) approximation are critically examined. For different values of density and spectral types of the central star, we have calculated the degree of ionization and the kinetic temperature of electrons as functions of distance from the central star, and compared them with the corresponding results of the OTS approximation. At most locations inside an HII region. the DFR ionizes considerable amount of hydrogen; therefore, the OTS approximation under-estimates the size of ionized regions. The exact treatment of the DFR transfer results in an about 10 to 20 percent increase in the classical S t r ¨ o m g r e n radius. The OTS approximation overestimates the local heating rate by raising the density of neutral hydogens. Consequently, it predicts higher values for the local electron temperature. The OTS approximation also exaggerates the dependence of electron temperature on density. When the hydrogen density is changed from 10 / c m 3 to 10 3 / c m 3 with an 06.5V star, the OTS approximation shows an about 3,000 K difference in the electron temperature, while the exact treatment of the DFR-transfer reduces the difference to about 1,000 K. The OTS approximation fails to demonstrate the brightening of the electron temperature close to the ionization boundary.

The performance of grass filter strips (GFS) in abating P04-P concentrations from the forage cropland was tested in an experiment on the 10% slope in Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration (RDA) from October 2007 to September 2009. Forage croplands with rye-corn double cropping system applied with chemical fertilizer and livestock manure (LM) were compared in a natural condition. The plots were hydrologically isolated Main plots consisted of the length of GFS, such as Om, 5m, 10m and 15m. Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). Concentrations of P04-P in surface runoff water were reduced as the length of GFS increased. Especially, GFS with 10m and 15m reduced PO4-P concentrations significantly compared to that with Om and 5m (p<0.05). The results from this study suggest that GFS improved the removal and trapping PO4-P from forage croplands.