In the present study, a novel ELISA method used recombinant nucleocapsid protein (rNP) as the coating agent. Recombinant Newcastle disease virus (NDV) protein was cloned and expressed in Escherichia coli. Though the rNP-ELISA results were consistent with commercial ELISA results for the NDV-negative sera samples, qualitatively and quantitatively variable (often reduced) results were obtained with NDV-positive sera. Although the rNP-ELISA results for NDV detection were inconclusive, further improvement and standardization of the rNP-ELISA approach, such as using multiple recombinant proteins as the ELISA coating agent and performing comprehensive statistical analyses of combined recombinant protein ELISA, should help counter Newcastle disease outbreaks by improving NDV detection.

목적 : 60세 이상 녹내장 환자의 시력, 입체시, 대비감도, 시야 결과를 분석하고자 하였다. 방법 : 본 연구에 참여 의사를 밝힌 고령자 중 정상안압녹내장에 해당하는 환자 총 18명(남5, 여13, 평균 나이 는 70.00±5.12세)을 대상으로 하였다. ETDRS(Early Treatment Diabetic Retinopathy Study)를 사용하여 측 정하였고, 입체시 검사는 티트무스 검사를 시행하였다. 대비감도 검사는 명소시 조건하에서 각각 3, 6, 12, 18 cpd (cycle per degree)의 주파수에 대해 검사하였다. 시야 평균지수는 정적시야계를 이용하여 측정하였고, 각각의 측 정값들의 상관성은 SPSS 21.0를 사용하여 분석하였고, 유의수준은 0.050 이하로 하였다. 결과 : 정상안압녹내장 환자군에서 좋은 눈의 평균지수 값은 –1.39±1.31 dB, 나쁜 눈의 평균지수 값은 – 3.59±2.34 dB 이였고, 티트무스 검사를 이용한 입체시 검사 결과 248.89±235.25초를 보였다. 고대비 시력은 0.11±0.11 LogMAR, 저대비 시력은 0.42±0.08 LogMAR를 보였다. Better MD는 입체시, 우안 저대비시력, 좌 안 대비감도 3 cpd와 의미 있는 음의 상관관계를 보였다(p<0.050). 결론 : 60세 이상 녹내장 환자에서 발생할 수 있는 시력, 입체시, 대비감도, 시야변화의 상관성을 확인할 수 있 었으며 정기적인 시기능 평가가 필요할 것으로 보인다.

Porcine epidemic diarrhea virus (PEDV) infects all-age pigs and causes enteric diseases. Genetic diversities in isolates been reported from each country, and those diversities highlighted in pathogenicity and vaccine. In this manuscript, we are reporting of new PEDV isolation in Korea, and with genetic characteristics. Our new isolate belongs to G2b and put the name as CNUP6-2018.

Exudative epidermitis (EE) is a generalized skin disease of pigs, mainly caused by Staphylococcus hyicus (S. hyicus). Antibiotic resistant S. hyicus leads to the failure of antimicrobial treatments. This necessitates proper identification of the strains in the field and determination of their antimicrobial susceptibility. This study was carried out to isolate Korean S. hyicus and determine its antimicrobial resistance. Isolate was sensitive to ceftiofur, ciprofloxacin, enrofloxacin, gentamicin, neomycin, and tylosin, but remarkably resistant to amoxicillin, lincomycin, penicillin, streptomycin, and tetracycline. Our study contributes to the understanding of the characteristics and antimicrobial resistance of Korean S. hyicus, which in turn will provide an antimicrobial treatment strategy to control EE.

Currently in Korea, standard operating procedure for the analyses of phenolic compounds in water is the spectrometric comparison of colors developed by 4-amino antipyrin with phenolic compounds. It is however that this method cannot identify individual compound and that some phenolic compounds do not react with 4-amino antipyrin. Spectrometric determinations of phenolic compounds were compared with chromatographic analyses of gas chromatography (GC) and high pressure liquid chromatography (HPLC) of various phenolic compounds. Individual phenolic compounds could be determined by both chromatographic methods but HPLC methods were more precise with lower detection levels in general.