This study was conducted to analyze long-term trends in moisture content and regional distribution characteristics of winter forages produced in Korea. A total of 14,204 samples collected from forage production farms and TMR facilities across 128 Si/Gun units nationwide from 2015 to 2024 were analyzed for moisture content, and annual, regional, and species-specific trends were examined accordingly. The overall mean moisture content was 34.82 ± 16.68%, with a declining trend from 46.70% in 2015 to 30.71% in 2024. Among the forage species, Italian ryegrass showed the lowest mean moisture content (29.44 ± 13.31%), decreasing consistently from 41.16% in 2015 to 23.91% in 2024, contributing substantially to the overall declining trend. Regional variation in moisture content was associated with differences in forage storage type composition, with Gangwon province showing higher moisture content corresponding to a greater proportion of silage, while Jeonbuk province showed lower moisture content with a higher proportion of hay. No statistically significant correlations were found between moisture content and environmental factors including precipitation and drainage installation rate (p>0.05), suggesting that final moisture content is likely influenced by multiple factors beyond regional precipitation, highlighting the need for further studies including direct variables like post-harvest management. The proportion of hay increased from 7.8% in 2015 to 32.3% in 2024, while silage decreased from 43.9% to 15.0%, a trend that coincides with the implementation of the national forage quality grading system initiated in 2015, suggesting a possible association. These results provide baseline data for developing region-specific forage production and quality management strategies in Korea.

Molecular markers have been widely utilized in population genetics, diagnostic taxonomy, and genetic mapping, and can be applied to cultivar discrimination during field selection processes for alfalfa. In this study, whole-genome sequencing information was obtained for seven alfalfa lines and cultivars developed in Korea, including ‘Alfaone (MS001)’, using Next-Generation Sequencing (NGS). Single nucleotide polymorphism (SNP) analysis revealed that ‘Alfaone (MS001)’ could be distinguished from other lines and cultivars using six SNP loci. Specifically, only two SNP loci were sufficient to differentiate ‘Alfaone (MS001)’ from major lines and cultivars such as ‘MS002’ and ‘Alfaking (MSCB07)’. This set of SNP barcodes provides a reliable standard for alfalfa cultivar discrimination, contributing to domestic cultivar protection and the advancement of the Korea forage industry. Furthermore, the development of distinguishing markers across alfalfa cultivars will enhance genetic resource identification and support the breeding of high-quality new cultivars.

Italian ryegrass (Lolium multiflorum Lam.) is a major winter forage crop requiring improved drought tolerance under climate change. This study evaluated 42 accessions, consisting of 20 Korean cultivars and 22 exotic germplasm under four polyethylene glycol (PEG) concentrations (0, 10, 15, and 20%) to assess germination and early seedling responses to drought stress. Increasing PEG levels significantly reduced germination percentage (GP), germination energy (GE), germination index (GI), and seedling vigor index (SVI), while mean germination time (MGT) increased. At 20% PEG, GP decreased by approximately 74% compared with the control. Early seedling vigor, expressed as SVI (integrating fresh weight, shoot length, and root length) also declined depending on concentration. Germination traits (GE and GI) showed strong positive correlations with SVI, indicating that early germination performance was closely associated with subsequent growth under drought stress. Fuzzy comprehensive evaluation and principal component analysis consistently identified several accessions (Earlybird, IR602, IR901, Florida 98, Maximus, Orlando, and Moscovsky 74) as relatively drought tolerant. Overall, the integration of germination and seedling vigor index provides a reliable phenotypic screening approach for identifying drought-tolerant Italian ryegrass.

This study demonstrated that inlet moisture content is a critical factor influencing drying efficiency, production rate prediction accuracy, and feed value changes of Italian ryegrass (IRG) in a multi-stage conveyor-type hot-air forage dryer. The experiment was conducted by uniformly loading IRG at a fixed drying height of approximately 25 cm with four different inlet moisture contents (30, 40, 50, and 60%). Drying time increased exponentially with increasing inlet moisture content, and a strong linear relationship was observed between inlet moisture content and the time required to reach 15% moisture, enabling the development of a reliable drying time prediction model. The proposed model showed high applicability and prediction accuracy at inlet moisture contents of 30–40%, where predicted and measured production rates were in close agreement. However, at inlet moisture contents of 60% or higher, the model substantially overestimated production rates, indicating limitations under high-moisture conditions. This behavior was attributed to reduced airflow penetration and diminished heat and mass transfer efficiency caused by increased material thickness and particle mass at high moisture levels. Feed value analysis revealed that crude protein content decreased by 12–20% following hot-air drying compared with fresh material, while ether extract, crude ash, and fiber components were not significantly affected. No significant differences in crude protein loss were observed among treatments with inlet moisture contents of 60% or lower. In conclusion, the multi-stage conveyor-type hot-air dryer and the proposed prediction model are suitable for drying Italian ryegrass at inlet moisture contents of 40% or lower and can be effectively applied under practical operating conditions. However, at inlet moisture contents of 60% or higher, improvements in airflow distribution within the dryer or the incorporation of moisture-dependent correction factors are required to enhance drying performance and prediction accuracy.

This study reports the development of a new alfalfa (Medicago sativa L.) variety, ‘Alfaone’, at the Forage Production Systems Division, National Institute of Animal Science, Rural Development Administration, Korea, from 2015 to 2023. The variety originated from an artificial cross between Xun Lu (maternal parent) and RadarⅡ Brand (paternal parent), followed by pedigree selection and performance testing. The elite line ‘MsCB01’ was subsequently released as ‘Alfaone’. Regional adaptability trials were conducted for two years (2022–2023) across four representative sites in Korea (Cheonan, Pyeongchang, Jeongeup, and Jinju) to evaluate agronomic traits, forage yield, and quality. Evaluated characteristics included plant height, regrowth ability, winter survival, and lodging resistance. The average dry matter yield of ‘Alfaone’ was 20,811 kg/ha, approximately by about 3% higher than that of the standard cultivar ‘Vernal’ (20,236 kg/ha). Yield superiority was particularly evident in Pyeongchang, suggesting excellent cold tolerance and winter hardiness. Assessment of forage nutritive traits indicated that ‘Alfaone’ was comparable to ‘Vernal’, demonstrating that its yield advantage did not come at the expense of quality. Overall, ‘Alfaone’ is a promising cultivar that combines high productivity with strong adaptability to unfavorable environments, particularly cold-prone regions. Its release is expected to promote the expansion of alfalfa cultivation, enhance forage self-sufficiency, and reduce dependence on imported hay in Korea.

A new barnyard millet (Echinochloa esculenta L.) cultivar, ‘Da-on’ (line BM3), was developed by the National Institute of Animal Science (NIAS) through pedigree selection using local germplasm collected from Jeju Island in 2016. After four years of line separation (2017–2020), a yield trial (2022), and regional adaptability tests across three sites (2023–2024), its agronomic performance and forage quality were evaluated. ‘Da-on’ is a mid-maturing cultivar with a heading date of August 5, which is 11 days later than the check cultivar ‘Borajik’. It exhibits an erect growth habit, purple panicles, and strong lodging resistance. The average plant height was 178.8 cm, which was 40.8 cm taller than that of ‘Borajik’. The dry matter yield of ‘Da-on’ was 16,858 kg/ha, representing a 130% increase compared with ‘Borajik’. Forage quality traits showed lower ADF (34.0%) and NDF (63.7%) contents, while total digestible nutrients (TDN) were higher (62.0%) than in the check. Crude protein content was comparable between the two cultivars. In addition, ‘Da-on’ showed resistance to lodging and leaf blight during field trials, confirming its stability across diverse environments. These results demonstrate that ‘Da-on’ is a promising summer forage crop cultivar suitable for nationwide cultivation, providing higher productivity and nutritive value to enhance forage self-sufficiency in Korea.

Field drying remains the primary bottleneck to consistent alfalfa haymaking under Korea’s humid, rain-prone summers. This study evaluated how dry matter (DM) yield and tedding frequency shape seasonal drying trajectories and leaf shatter in Cheonan during 2024. The experiment was conducted in May, June, August, September, and October 2024 with three DM yield levels (3, 5, and 7 ton/ha) and three tedding regimes (no tedding, once daily, and twice daily). Favorable May–June weather enabled swaths to reach <20% moisture within 100 h, whereas in September–October, moisture commonly remained ≥50%, indicating conditions unsuitable for dry hay. Yield effects on drying were generally small except in August, when re-wetting events accentuated between-group differences. Tedding effects were season-dependent: treatment differences emerged after rainfall in August and were evident in September, with twice-daily tedding producing the lowest moisture among tedding treatments. Leaf dropout rose with greater tedding frequency and higher DM yield; significant tedding effects were detected in June and September, and twice-daily tedding often exceeded no-tedding for leaf losses, particularly at 7 ton/ha. Collectively, omitting tedding is a viable option for May–June hay (4 days to ~20% moisture), while for post-June harvests, tedding offers limited drying benefits and may elevate leaf shatter; ensiling is therefore recommended when drying conditions are suboptimal.

Climatic change affects agricultural, environmental and livestock production. Forage productivity is highly dependent on the weather change. The Pyeongchang region has more difficulty in cultivation of Sorghum-Sudangrass hybrids (SSH) than other warm regions because of the cold weather. This experiment was carried out to analysis the agronomic characteristics, productivity, and nutritive value of three cultivars of Sorghum-Sudangrass hybrids in the Pyeongchang region from 2021 to 2022. Two harvests were taken in each year, and the agronomic characteristics, dry matter yield, and nutrient content of the whole SSH crops were determined. The plant height ranged between 281-291 cm and 165-172 cm in the first and second harvest, respectively. Plant height of Superdan (291±49 cm) was the highest in the first cut and Dairy mens Dream (172±30 cm) was the highest in the second cut time. Stem diameter ranged from 9.5 to 8.8 mm with Superdan having a higher level than the other two cultivars. The Dairy mens Dream variety produced significantly higher dry matter yield (15,695 kg/ha) than those of Superdan (14,584 kg/ha) and Supergreen (14,300 kg/ha) over a two-year experimental period. In terms of nutritional quality, the crude protein content, neutral detergent fiber and acid detergent fiber of SSH cultivars were analysed. There were no significant changes in the content of CP, ADF and NDF ranges among the cultivars. In addition, the Superdan cultivar had slightly higher CP content (10.17%) and was followed by Dairy mens Dream (9.84%) and Supergreen (9.54%) in the second cut time of 2-year average. Dairy mens dream had lower ADF and NDF values than other cultivars; however, no significant differences amongst cultivars in fiber content were observed. Therefore, these three cultivars displayed the potential growth characteristics, DM yield and nutritive values in Pyeongchang region. Hence, the SSH cultivars have a potential to withstand the climatic change and improve SSH productivity in the study area.

Cool-season grasses are currently widely used in South Korea, but their productivity and quality may decline due to global warming associated with climate change. Warm-season grasses have shown greater adaptability to heat stress and drought conditions, making them potential alternatives for summer pasture establishment in central and southern Korea. This study aimed to compare the growth characteristics, productivity, and forage value of warm-season grasses bermudagrass and crabgrass for pasture establishment in the central region of South Korea. The experiment was conducted from May 2023 to October 2024 at the National Institute of Animal Science, Cheonan, South Korea. Crabgrass exhibited superior initial establishment and faster growth rates, whereas bermudagrass had poor initial growth, placing it at a competitive disadvantage against weeds. However, after the first cutting, bermudagrass successfully established itself and maintained a stable botanical composition. The annual dry matter productivity of crabgrass (8,586 kg/ha in 2023 and 12,013 kg/ha in 2024) was 32.62% and 40.18% higher, respectively, than that of bermudagrass (5,785 kg/ha in 2023 and 8,570 kg/ha in 2024). In forage value, crabgrass tended to show higher feed value at the initial harvest, while bermudagrass showed higher quality at the later harvests. In conclusion, these results suggest that both crabgrass and bermudagrass have potential for pasture establishment, with carbgrass being more suitable for short-term due to its rapid growth and high productivity, and bermudagrass being more appropriate for long-term due to its stable productivity in regions where overwintering in possible.

Near infrared reflectance spectroscopy (NIRS) is widely used to assess the nutrient composition of forages. In forage, the leaf to stem ratio of alfalfa greatly affects its forage quality, with a high ratio of leaf indicated as high quality. This study aimed to evaluate the predictability of the alfalfa leaf-to-stem ratio and feed value using NIRS. Alfalfa hay was manually separated into leaves and stems by hand and the analysis samples were then made in the controlled range between 0 and 100%. Calibration models (n=320) were developed using modified partial least squares regression (MPLS) based on cross-validation. The optimal calibrations were selected based on the highest coefficients of determination in cross-validation (R2) and the lowest standard error of cross-validation (SECV). The prediction accuracy for the leaf-to-stem ratio (SECV, 5.95 vs. 5.71%; R2, 0.91 vs. 0.91) in alfalfa hay was comparable. For leaves, the standard error of calibration (SEC) was 4.94% (R2=0.94), and for stems, it was 4.81% (R2=0.94). The leaves and stems of the SEC were 4.94% (R2=0.94) and 4.81% (R2=0.94), respectively. The prediction accuracy for feed value, based on the leaf-to-stem ratio, predicted SECV values of 0.92% (R2=0.88) for crude protein (CP) content, 1.92% (R2=0.91) for neutral detergent fiber (NDF) content, 1.36% (R2=0.91) for total digestibility nutrient (TDN) content, and 9.86 (R2=0.81) for relative feed value (RFV). The results of this study demonstrate the potential of the NIRS method as a reliable tool for predicting the leaf-to-stem ratio of alfalfa hay, and show available techniques for routine feed value evaluation.

Forage crop cultivation and management are the greatest challenge under warm and dry climatic conditions. In this study, we estimated the productivity of three Sorghum-Sudangrass hybrids (SSH) cultivars in Cheonan, Korea, under different weather conditions during 2021-2023. The selected three cultivars performed well in the first and second cutting time during the experimental period and the plant growth characteristics were slightly different among cultivars. Particularly, the plant height was highest in Superdan cultivars (282 ± 24, 271 ± 30 cm), followed by Dairy mens dream (263 ± 39, 283 ± 29 cm) and Supergreen (270 ± 36, 264 ± 34 cm), for the first and second cuts respectively. The stem diameter slightly decreased in the second cut compared with the first cut of SSH cultivars. The highest stem diameter was found in Superdan cultivars (11.1 ± 1.7 mm), greater than Supergreen (10.2 ± 1.7 mm), and Dairy mens dream (9.5 ± 1.8 mm). Also, the total dry matter yield (DMY) was highest in Dairy mens dream (28,868 ± 6,653 kg/ha) followed by Supergreen and Superdan cultivars. In 2021 and 2023, the highest plant height, stem diameter and DMY were measured in the selected cultivars compared to 2022. The crude protein level was higher in the first cutting of all three cultivars at approximately 9-12%, in the order of Supergreen > Dairy mens dream > Superdan varieties. Moreover, the crude protein content was lowest in the second harvest of all cultivars, but the NDF and ADF levels did not alter in both harvest periods across different cultivars and years. In conclusion, the selected cultivars for SSH forage production could be efficient and recommended in the Cheonan region. However, the choice of cutting time and optimum precipitation should be considered to further increase SSH forage cultivation.

A new variety of Alfalfa (Medicago sativa L.), named 'Alfaking' was developed between 2015 and 2023 at the Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, Republic of Korea. The variety was produced through artificial hybridization, with ‘Paravivo’ serving as the maternal line and ‘WL514’ as the paternal line. ‘Alfaking’ underwent field tests across four regions (Cheonan, Pyeongchang, Jeongeup, and Jinju) to evaluate its agronomic characteristics and forage production over two years (2022-2023). The dry matter yield of ‘Alfaking’ reached 22,516 kg/ha, which is 11% higher than the control variety, ‘Vernal.’ ‘Alfaking’ exhibited 2.1% higher crude protein content than ‘Vernal’ in forage nutritive value. The development of this new alfalfa variety, which exhibits excellent adaptability to challenging environmental conditions, is expected to enhance forage cultivation and productivity in Korea.

The alfalfa weevil (Hypera postica) is an important pest that causes significant damages to alfalfa crops, reducing yield and quality, but there’s a solution. This research had two main goals to evaluate the efficacy of insecticides available in Korea for alfalfa weevil control and to provide data for pest management studies. The experiment, conducted from 2023 to 2024 at the National Institute of Animal Science in Cheonan, Republic of Korea, included four treatment plots: control (CON), early-occurrence (EAR), mid-occurrence (MID), and late-occurrence (LAT). It also included two frequency plots with primary and secondary insecticide using 50% fenitrothion emulsion, which made it truly comprehensive study. The primary insecticide was applied at the first observation of the alfalfa weevil larvae, with subsequent secondary applications at specified intervals. The results showed that two-times insecticide applications significantly reduced larvae populations and increased yield and nutrient content compared to a single application. Specifically, control rates ranged from 94 to 94.7% on the third day after treatment and from 72.2 to 93.4% on the seventh day. Plots with two applications had higher yields and crude protein content. The study concluded that the timing and frequency of insecticide applications are critical to maximizing alfalfa yield and quality, emphasizing the importance of optimized application strategies for effective pest control.

Corn is essential for both humans and animals. The crop is the third most important food crop after rice and wheat. A rise in farming is resulting in a lack of self-sufficiency of high quality forages. Therefore, corn cultivars in Korea must be optimized to increase productivity and yield. A study was conducted in Pyeongchang, South Korea between 2021-2022 to evaluate the characteristics and productivity of three corn cultivars: Kwangpyeongok, AGR 41, and Nero IT. Different varieties of corn were sown in Pyeongchang on April 29 and April 27 of 2021 and 2022, respectively. The productivity and characteristics of the cultivars Kwangpyeongok, AGR 41, and Nero IT were evaluated during the yellowing ripening stage. The characteristics included the leaves, stems, grain, and stover content. Across different corn cultivars, there was no significant difference in stem height, diameter, forage or grain quantity based on an average of two years. Yet, significant differences were noted in the stem heights of all cultivars between 2021 and 2022 (p<0.05). There was a significant difference in the diameters of Kwangpyeongok and Nero IT between 2021 and 2022. A significant difference in forage yield was observed for Nero IT between 2021 and 2022. The yield of grains and forages was higher in 2021 than in 2022, which could be attributed to temperature and precipitation variations. We can conclude from these data that all cultivars in Pyeongchang showed significant productivity and yield, which could be useful to produce nutrient rich silage for livestock.

Sorghum-Sudangrass hybrids (SSH) is a grass cereal hybrid crop with a high yield potential under different climatic conditions. The aim of this study was to evaluate the growth characteristics, dry matter yield and nutrient content of the SSH cultivars such as Dairy Mens Dream, Superdan and Supergreen from 2021 to 2023 in Jeju Island, Korea. Among the three cultivars, Superdan grew significantly taller with a larger stem diameter at both harvest times. In contrast, Dairy Mens Dream had least height and less stem diameter, but the dry matter yield was greater for Dairy Mens Dream than other cultivars. Furthermore, the crude protein content at the first cut was higher than 8.5% compared to the second cut (6.5 – 7.0%). The neutral detergent fiber (NDF) and acid detergent fiber (ADF) content was higher in the second harvest of all cultivars compared to the first harvest of SSH in 2021. The SSH cultivation in 2021 and 2023 had higher ADF and NDF content than in 2022, possibly due to the average precipitation and optimum temperature suitable for SSH production in 2021 and 2023. The selected three SSH cultivars showed significant growth characteristics, dry matter content, and nutritional value. The overall data suggested that all three SSH showed significant productivity and nutritional content in the Jeju region of sub-tropical climatic condition.

This study aims to contribute to resolving the critical issue of weed management in newly established alfalfa fields, study has been conducted on effective herbicide use. The study evaluated the impact of various domestically available foliar herbicides on alfalfa phytotoxicity, weed control, yield, and nutritive value. The experiment was designed in a randomized complete block design with four treatments. Alfalfa ‘SW 5615’ seeded in the spring of 2024 on a 1 ha field (March 18), with herbicide treatments including fluazifop-P-butyl (FPB), bentazone (BEN), and a mixture of these herbicides (MIX). Herbicide efficacy, alfalfa yield, and nutritive value were assessed 30 days post-application. Results indicated that the MIX treatment achieved superior weed control comparable to hand weeding (HW), although it exhibited higher phytotoxicity, requiring extended recovery periods. While MIX led to lower overall yield, it enhanced alfalfa purity, resulting in higher crude protein (CP) content and relative feed value (RFV) compared to other treatments. The study concludes that despite the potential for increased phytotoxicity, mixed herbicide treatments could offer a strategic advantage in enhancing the quality of alfalfa feed through effective weed management, thereby improving CP and RFV, critical factors for the nutritional value of alfalfa. These findings provide valuable insights for optimizing weed management practices in alfalfa cultivation, suggesting that mixed herbicide application, although associated with increased phytotoxicity on the plants, could improve the overall feed quality by reducing weed competition.

This study was conducted to find a way to improve quality by observing changes in quality and microbial communities according to whether corn silage was treated with additives and the storage period, and to utilize them as basic research results. The experimental design was performed by 2˟4 factor desigh, and the untreated (CON), and the additive inoculated (ADD) silage were stored and fermented for 30 (TH), 60 (ST), 90 (NT), and 120 (OHT) days, with each condition repeated 3 times. There was no change in the nutrient content of corn silage according to additive treatment and storage period (p>0.05). However, the change in DM and the increase in the relative proportions of lactic acid content and Lactobacillales according to the storage period (p<0.05) indicate that continuous fermentation progressed until OHT days of fermentation. Enterobacterales (33.0%), Flavobacteriales (14.4%), Sphingobacteriales (12.7%), Burkholderiales (9.28%) and Pseudomonadales (6.18%) dominated before fermentation of corn silage, but after fermentation, the diversity of microorganisms decreased sharply due to the dominance of Lactobacillales (69.4%) and Bacillales (11.5%), Eubacteriales (7.59%). Therefore, silage maintained good fermentation quality with or without microbial additives throughout all fermentation periods, but considering the persistence of fermentation even in long-term storage and the aerobic stability, it would be advantageous to use microbial additives.

This study was conducted to determine the optimal spring seeding dates for alfalfa yield and feed value. The experiment was conducted annually for three years (2021~2023) at the field in the Department of Animal Resources Development, NIAS, located in Cheonan. The treatments involved six seeding dates ranging from February 24 to April 14, with 10days intervals. Alfalfa was harvested four times a year at the early flowering stage. Dry matter yield showed a tendency to decrease with delayed the seeding date. However, depending on the climatidc condisions in the seeding year, the dry matter yield on March 14 or 24 was comparable to that on February 24. Annual dry matter yield varied, influenced by the daylight conditions each year. The average feed value did not significantly differ within in the same year with delayed seeding dates (p>0.05). Therefore, the most stable period for alfalfa spring seeding in the central area of South Korea is considered to be from February 24 to April 4, with February 24 indentified as the optimal date.

Understanding changes in fermentation characteristics and microbial populations of forage silage during ensiling is of interest for improving the nutrient value of the feed for ruminants. This study was conducted to investigate the changes in fermentation characteristics and bacterial communities of whole crop rice (WCR) silage during the ensiling period. The chemical compositions, pH, organic acids and bacterial communities were evaluated at 0, 3, 6, and 12 months after ensiling. The bacterial communities were classified at both the genus and species levels. The dry matter content of WCR silage decreased with the length of storage (p<0.05), but there was no significant difference in crude protein and NDF contents. Following fermentation, the pH level of WCR silage was lower than the initial level. The lactic acid content remained at high levels for 3 to 6 months after ensiling, followed by a sharp decline at 12 months (p<0.05). Before fermentation, the WCR was dominated by Weissella (30.8%) and Pantoea (20.2%). Growth of Lactiplantibacillus plantarum (31.4%) was observed at 3 months after ensiling. At 6 months, there was a decrease in Lactiplantibacillus plantarum (10.2%) and an increase in Levilactobacillus brevis (12.8%), resulting in increased bacteria diversity until that period. The WCR silage was dominated by Lentilactobacillus buchneri (71.2%) and Lacticaseibacillus casei (27.0%) with a sharp reduction in diversity at 12 months. Overall, the WCR silage maintained satisfactory fermentation quality over a 12-month ensiling period. Furthermore, the fermentation characteristics of silage were found to be correlated to bacterial microbiome.

Pasture formation and management are crucial to avoid yield reduction. This experiment aimed to examine the effects of tall fescue-centered mixed-seeding combinations on yield and vegetation changes in perennial pastures in the central region for two years, from September 2020 to October 2022. The treatments were arranged in three replications in a randomized block design: control (C), tall fescue-based mixture-1 (T-1), and tall fescue-based mixture-2 (T-2). The tall fescue (TF), orchard grass (OG), perennial ryegrass (PRG), Kentucky bluegrass (KBG), and white clover (WC) were used. The emergency rate of grasses (70.0 to 73.3%) did not differ among mixed seeding combinations. Overwintering rates (81.7 to 83.3%) were similar among treatments. The plant height of grasses was similar at each harvest date, with the highest height (86.2 cm) recorded in the second harvest of the first year, followed by that (58.4 cm) in the third harvest of the first year; it was least (38.9 cm) in the fourth harvest of the second year. There was no significant difference in the dry matter yield of grasses among the mixed seeding combination treatments in the first, third, or fourth harvests of the first year (p>0.05). For second-year grasses, dry matter yield was not significantly different in harvest date among the treatments (p>0.05). Based on mixed seeding ratio, orchard grass showed the highest yield at 70% in the C treatment, followed by tall fescue at 80% and 60% in the T-1 and T-2 treatments, respectively, in the first harvest after seeding. There was no significant difference in feed value between treatments (p>0.05), but a significant difference was observed between the third and fourth harvest (p<0.05). Therefore, it indicated that it is important to create perennial pastures in the central region through mixed seeding combinations centered on tall fescue.