수입된 관상용 부추속 종과 야생 부추속 종을 교배하고 새로운 품종을 얻기 위해서는 한국에 자생하는 부추속 종의 개화생리의 이해가 필요하다. 본 연구는 일장처리를 통해 자생 부추속 4종의 화아발달 및 생육특성을 알아보고자 수행하였다. 실험은 국립수목원 유용식물증식센터의 Phyto-Garden System에서 실시되었으며, 온도는 25℃, 습도는 70%로 유지 되었다. 꽃눈이 각 1개씩 출현한 개체를 이용하여, 2019년 6월 28일에 장일(LD, 16hours), 단일(SD, 9hours), 외부환경으로 나누어 실험하였다. LD와 SD처리에서 8:00~17:00(9h)까지 는 296±13μmol･m-2 ･s-1의 광도 하에서 실험하였다. 이후의 추가되는 시간은 형광등을 이용해 약 5μmol･m-2 ･s-1로 일장연장 (day extension, DE)처리를 하였다. 생육조사는 생장특성으로 초장, 초폭, 엽수, 엽장을, 개화특성으로는 꽃의 수와 개화소 요일수를 조사하였다. 실험결과, 한라부추의 단일처리에서 엽수를 제외한 초장, 초폭, 엽장, 엽폭이 장일처리 및 외부환경 보다 높게 측정되어, 4종의 부추속 식물 중 한라부추가 추대 이후 단일처리에서 가장 생장이 왕성한 것으로 확인하였다. 또한, 단일처리에서 개화소요일수는 각각 강부추 51.8일, 둥근 산부추 46.5일, 선부추 43.7일 그리고, 한라부추 52일로 나타 났다. 반면 장일처리의 개체들은 실험종료일까지 전혀 개화하지 않았다. 외부 환경조건에서는 부추속 4종이 평균적으로 97.2일째에 개화하였다. 본 실험은 자생 부추속 4종이 단일처리에서 외부환경보다 화아발달이 촉진되는 결과를 나타냈다.

Three species of the genus Neolygus Knight - N. hakusanensis (Yasunaga, 1991), N. roseus (Yasunaga, 1991) and N. zhugei (Yasunaga, 1991) - are recognized for the first time in Korea. An identification key to the eleven Korean Neolygus species is presented. Some illustrations of male genitalic structures are also provided.

In this study, a load duration curve was applied to the Jangseongcheon, one of the tributaries of the Yeongsan River, to assess whether the target water quality was achieved. In addition, pollution of the water body was investigated to develop and suggest the optimal management time with respect to polluted flow sections and monthly conditions. The average flow rates of sections JS1 and JS2 were 0.25 m3/s and 1.08 m3/s , respectively. The BOD and T-P for water-quality standards at JS1 were rated at II, whereas the COD and TOC were rated at III, thus indicating a fair level of water quality. By contrast, the BOD at JS2 was rated at III, the T-P at IV, and the TOC at V, indicating poor water quality in this section. The load duration curve was plotted using the actual flow data measured in eight-day intervals for eight years from 2011 to 2018 at locations JS1 and JS2 in the Jangsungcheon Basin. In an assessment using the load duration curve on whether the target water quality was met at location JS1, all of the water quality parameters (BOD, COD, TOC, T-N, T-P, and SS) satisfied the target water quality. By contrast, at location JS2, parameters COD, TOC, T-N, and T-P exceeded target values by more than 50%, indicating the target water quality was not met. The discharge loads of locations JS1 and JS2 were analyzed to identify the reasons the target water quality was exceeded. Results revealed that the land system contributed considerably. Furthermore, the discharge load of JS2 accounted for more than 80% of the load on the entire basin, excluding that of JS1. Therefore, the best method for restraining the inflow of pollutants into the stream near location JS2 must be applied to manage the water quality of the Jangsungcheon.

Genome sequencing researches for considerable numbers of crops and wild plants are being developed. Cytogenetic researches according to chromosome number and size are essential to confirm and comprehend ploidy level and genome size before genome sequencing project is actually conducted. Cytogenetic researches on six food crop plants were carried out by DAPI staining and fluorescence in situ hybridization (FISH) method. Fagopyrum esculentum Moench showed 2n=2x=16, each chromosome length of 1.42㎛ to 1.77㎛, total chromosome length of 13.31㎛, and karyotypic formula of 2n=8m; Phaseolus angularis W.F. Wight, 2n=2x=22, 2.01㎛ to 3.84㎛, total 28.03㎛, 2n=9m+2sm, Perilla frutescens var. japonica Hara, 2n=2x=40, 1.73㎛ to 2.76㎛, total 44.36㎛, 2n=5m+13sm+2st. Chromosome sizes of the other three species such as, Panicum miliaceum L., 2n=2x=36, total chromosome length of 30.83㎛, Sesamum indicum L., 2n=2x=26, 27.39㎛, lpomoea batatas L., 2n=2x=30, total 33.51㎛ were too small for each chromosome type to be identified and analyzed. The result of FISH analysis using 5S and 45S rDNA probe showed species-specific chromosome locations in the genome. These preliminary analyses were carried out to decide which food crop to prioritize for genome sequencing. This work was supported by the “Cooperative Research Program for Agriculture Science & Technology Development (No.PJ009837), Rural Development Administration, Republic of Korea.