스탠다드국화 신품종을 육성하기 위하여 충남농업기술원 화훼연구소에서 2009년에 황색의 모본 ‘Summer Yellow’와 부본 ‘ST07-09-02’계통을 인공 교배하였다. 2010년에 종자를 파종하였고, 이중 내병성이 강하고 기호성이 우수한 개체를 선발하여 ‘ST10-047-01’로 계통명을 부여하였다. 2011년부터 2013년까지 주년 생산성을 위해 촉성 및 자연, 억제재배 특성을 각각 검정하였으며, 2013년 ‘Geumhwa’로 명명하고 품종등록 출원하였다. ‘Geumhwa’의 생육 및 개화특성은 국내에서 많이 재배되고 있는 황색 스탠다드국화인 ‘Summer Yellow’를 대조 품종으로 하여 조사하였다. ‘Geumhwa’ 품종은 자연개화기가 10월 6일로 ‘Summer Yellow’의 10월 25일에 비해 빨랐다. ‘Geumhwa’는 초장이 86.3cm로 ‘Summer Yellow’의 93.8cm보다 작았고, 곁가지 제거수는 8.1개로 ‘Summer Yellow’의 16.6개보다 적었다. ‘Geumhwa’의 꽃직경은 13.6cm로 ‘Summer Yellow’의 13.5cm와 비슷하였으며, 꽃잎수는 ‘Geumhwa’가 263.6개로 ‘Summer Yellow’의 295.3개보다 적었다. 재배상 유의사항은 ‘Geumhwa’는 중간종이므로 초기생육이 왕성하도록 비배 관리를 하고 생육기간 중 지베렐린 1,000mg･L-1를 2회 처리하여 신장력을 높이도록 한다. 또한 설상화수가 적은 편이므로 재전조를 실시하여 설상화수를 늘린다면 황색의 연중 조기개화가 가능한 고품질 신품종 스탠다드 절화국화로써 소비자 기호 충족 및 농가소득 창출에 기여할 수 있을 것으로 기대된다.

S. aureus is reported as a major cause of nosocomial infections after dental care and involved in endocarditis, bacteremia, osteomyelitis, peritonitis, and soft tissues etc. It is very important to identify the distribution and the diversity of toxin gene associated with the S. aureus expression in dental care patients with periodontitis directly for an effective prevention and treatment of dental diseases. Fifty four strains of S. aureus were isolated from the saliva of 129 patients who were diagnosed with periodontitis at dental clinics and hospitals located in Seoul. The distribution of the virulence gene and the genetic diversity of the strains were studied using the polymerase chain reaction with isolated strains. The enterotoxin test showed Seb was the most frequent gene with 88.9%. The hemolysin gene of Hla, Hib and Hld were the most frequently gene with 98.1% (53 strains), leukocidins gene of lukM showed 90.7% (49 strains), and laminin binding protein gene of Eno showed 100% (54 strains), respectively. The diversity of the enterotoxin gen was held as Seb-Seg-Sei gene of 35.2% (19 strains), the diversity of hemolysin gene of Hla-Hlb-Hld gene was 98.1% (53 strains) and the diversity of leukocidins gene of LukD-LukM were 88.9% (48 strains), respectively. Patients with dental disease showed somehow high toxin gene expression so that S. aureus in dental care area is judged to show very highly pathogen with a high and infection rate. In the future, additional studies for these toxin genes seem to be required.

The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, SeminarkPro (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at 17℃ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/Dead® stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at 17℃) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90～6.86) and T4 (6.86～6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0～7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 (20×106 sperm cells/ml : 5×105 cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.

The purpose of the present study was to examine the nutrient intake status of elderly dementia patients. We surveyed the dietary intake of 50 demented elderly patients receiving a regular diet, who were hospitalized in a geriatric hospital in Yongin, Gyeonggi-do. The average age of the subjects was 79.6 土6.5 for the males and 80.5 土6.3 for the females. The average heights were 169.5 土6.0 cm (males) and 154.6 土5.2 cm (females), and the average weights were 58.5 土7.4 (males) and 51.7 土8.9 kg (females). Depending on the type of dementia, the male patients showed significant differences in their intakes of vegetable protein, fiber, total iron, and non-heme iron between the Alzheimer’s disease (AD) and the vascular dementia (VD) groups, and the female patients showed significant differences in their intakes of total protein, phosphorus, zinc, and vitamin B6 between the two groups. The male patients showed significant differences in their intakes of animal-source calcium and carotene according to ability to self-feed. According to physical activity, the male patients showed significant differences in vegetable fat and vitamin A intake, and the females showed significant differences in their intakes of total protein, animal protein, fiber, ash, total calcium, vegetable-source calcium, animal-source calcium, phosphorous, total iron, non-heme iron, heme iron, sodium, vitamins B1, B2, B6, niacin, vitamin C, folate, and potassium. We found that the patients consumed excessive protein, but low amounts of calcium, vitamin B2, and folate. Also, the patients’ physical activity abilities appeared to affect their nutrient intakes.