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        검색결과 18

        2.
        2019.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The poultry red mite (PRM), Dermanyssus gallinae, is one of the most important external parasites in the poultry industry. PRM adheres to birds’ bodies, and its hematophagy causes anemia and itching, and reducing egg production (~20%). Fipronil is a toxic pesticide used to control PRM. Thus, an environmentally friendly alternative control agent is needed. In this study, the effectiveness of various environmentally friendly PRM control measures including an acaricide consisting of synthetic silica, clove extracts, garlic extracts, and a probiotic solution were evaluated, comparing them to a combined treatment using the chemical acaricides. Each agent was tested in vitro using two approaches, in which PRMs were either added to environments containing acaricide, or sprayed in situ. Within 10 hours of inoculation, all the environmentally friendly acaricides except for the probiotic solution killed or rendered immotile significantly more mites than the control, normal saline (p < 0.05). The performance of the environmentally friendly acaricides, except for synthetic silica, was significantly weaker than that of commercial chemical acaricides. (p < 0.05). Further tests to determine the optimal concentration of amorphous synthetic silica agent for complete PRM control found this to be 400 g/L. PRM motility following treatment showed the same pattern: the synthetic silica agent and chemical acaricide killed mites, while application of other environmentally friendly agents resulted in living, but immotile, insects, or those with reduced motility. As a result, these environmentally friendly acaricides, especially the synthetic silica agent, could be used as alternatives to chemical acaricides for PRM control.
        4,000원
        3.
        2018.10 구독 인증기관·개인회원 무료
        친환경 해충 방제 방법 중 LED를 비롯한 빛을 이용한 해충방제가 활발하게 연구되고 있으며, 해양, 농장, 축사 등 산업적인 측면뿐만 아니라 사회 전반적인 분야에서 활용되고 있다. 해충 기피램프의 광원은 주로 LED(550~630mm)가 활용되고 있으나 출력이 약해 넓은 면적에 적용이 힘든 단점이 있다. 이에 제논램프를 이용한 넓은 면적에 적용 가능한 식물재배용 해충 기피램프를 적용하여 담배거세미나방에 의한 작물 및 피해과실률을 비교하였다. 특정 파장(870~920mm, 자외선:가시광선:적외선=2:34:64)을 방출하는 제논램프를 높이 2.5m, 2/10a개 설치하였다. 광원의 처리는 3수준(일몰 후 2시간 경과 시점부터 2시간, 4시간, 무처리)으로 하였으며, 나방의 유입을 원활하게 하기 위하여 모든 출입구를 개방하였다. 과실 피해가 발생한 후 출입구를 폐쇄하여 제논램프가 유입된 나방의 작물 및 과실 가해를 억제하는지 조사하였다. 토마토 재배는 경상남도 함안(35° 15’ 0”N / 128° 25’ 0”E)의 유리온실(6*10*5m)에 토마토(데프니스, 유럽종)를 재식간격 1m(줄 간격), 0.5m(포기사이거리)재배하였다. 생육기간은 4월 12일 정식하여 8월 2일까지이다. 토마토 재배기간 동안 격주간격으로 생육(초장, 엽면적, 잎, 과실, 줄기의 생체중 및 건물중)조사를 하였으며, 매주 피해과, 정상과의 수량을 조사하였다. 조사 결과 대조구에서 엽의 건조 중량이 21%정도 낮았으며, 줄기의 건조 중량은 2.5배 높았으며, 초장, 엽면적은 차이가 없었다. 제논램프 처리 시간에 따라 피해과실률((피해과실 중량(g)/총 과실 중량(g))*100)은 제논램프 4시간 : 초기 7.59 → 2주 후 0.02a, 2시간 : 초기 3.41 → 2주 후 6.32b, 대조구 초기 10.00 → 2주 후 42.71c% (Analysis of variance, p < 0.1) 로 조사되었다. 대조구에서는 유입된 담배거세미나방이 온실 내에서 번식함에 따라 엽의 생체중은 감소하였으나 엽면적에서는 유의한 차이를 보이지 않았는데, 이는 대조구의 잎이 심하게 황화된 경우가 많았고, 이 때문에 엽면적은 증가하였으나 중량감소 하였다. 제논램프 2시간, 4시간 처리에서는 담배거세 미나방에 의한 작물 및 피해과실률이 감소하였으며, 제논램프 4시간 처리에서 유의하게 높은 방제 효과를 보였다.
        5.
        2018.06 KCI 등재 서비스 종료(열람 제한)
        In this paper, a high performance underwater vehicle which can be manufactured at low cost is designed and fabricated, and its performance is verified through experiments. To improve efficiency, the Myring equation is used to design the appearance and the duct structure including the thruster is planned to increase the propulsion efficiency while reducing the drag force. Through various methods, it is secured stable waterproof performance, and also is devised to have high speed movement and turning performance. The developed underwater vehicle is equipped with a high output BLDC motor to achieve a linear speed of up to 2 m/s and can change direction rapidly with stability through four rudders. The rudders are driven by coupling a timing belt and a pulley by extending the axis of a servo motor, and are equipped at the end of the body to turn heading. In addition, for stable posture control, the roll keeps its internal center of gravity low and maintains its stability due to restoring force. By controlling the four rudders, pitch and yaw are handled by the PID controller and show stable performance. To investigate the horizontal turning performance, it is confirmed that the yaw rate controller is designed and stable yaw rate control is performed.
        6.
        2016.08 KCI 등재 서비스 종료(열람 제한)
        For the underwater localization, acoustic sensor systems are widely used due to greater penetration properties of acoustic signals in underwater environments. On the other hand, the good penetration property causes multipath and interference effects in structured environment too. To overcome this demerit, a localization method using the attenuation of electro-magnetic(EM) waves was proposed in several literatures, in which distance estimation and 2D-localization experiments show remarkable results. However, in 3D-localization application, the estimation difficulties increase due to the nonuniform (doughnut like) radiation pattern of an omni-directional antenna related to the depth direction. For solving this problem, we added a depth sensor for improving underwater 3D-localization with the EM wave method. A micro scale pressure sensor is located in the mobile node antenna, and the depth data from the pressure sensor is calibrated by the curve fitting algorithm. We adapted the depth(z) data to 3D EM wave pattern model for the error reduction of the localization. Finally, some experiments were executed for 3D localization with the fast calculation and less errors.
        7.
        2014.07 서비스 종료(열람 제한)
        The legume family is the third largest group, including approximately 650 genera and 18,000 species, in the flowering plants and the second important crops to the Poaceae in the agricultural economy. Comparative analysis is a useful tool to understand cross-species genomic structure and alterations during organism’s evolutionary history. In this study, we constructed a composite comparative map of ten legume species, including Medicago truncatula, Medicago sativa, Lens culinaris, Pisum sativum, Lotus japonicus, Cicer arietinum, Vicia faba L, Vigna radiata, Phaseolus vulgaris and Glycine max. Of these species, M. truncatula, which is a representative model system, played a central role to develop the cross-genome amplifiable PCR gene markers for the purpose of transferring them to other related legume species. A total of 140 cross-species core markers were employed to analyze genomic colinearity across this broad array of legume species. The comparative map demonstrates a diverse array of evolutionary events, such as duplications, inversions and reciprocal translocations. It is anticipated that resulting maps would provide a broader insights into the lineage-specific genomic organization of these glalegoid/phaseoloid legumes, which are two clades containing almost all crop legumes of economic importance, and can further used for the molecular breeding through translating genomic information into other orphan legumes.
        8.
        2014.07 서비스 종료(열람 제한)
        Susceptible Vitis vinifera responds to Xylella infection with a massive redirection of gene transcription. This transcriptional response is characterized by increased transcripts for phenlypropanoid and flavonoid biosynthesis, ethylene production, adaptation to oxidative stress, and homologs of pathogenesis related (PR) proteins, and decreased transcripts for genes related to photosynthesis. In addition, the results suggest that susceptible genotypes respond to Xylella infection by induction of limited, but inadequate, defense response. We also compared the transcriptional and physiological response of plants treated by pathogen infection, low or moderate water deficit, or a combination of pathogen infection and water deficit. Although the transcriptional response of plants to Xylella infection was distinct from the response of healthy plants to moderate water stress, we observed synergy between water stress and disease, such that water stressed plants exhibit a stronger transcriptional response to the pathogen. This interaction was mirrored at the physiological level for aspects of water relations and photosynthesis, and in terms of the severity of disease symptoms and pathogen colonization, providing a molecular correlation of the classical concept with the disease triangle.
        9.
        2014.07 서비스 종료(열람 제한)
        Comparative analysis is a typically useful tool for translating genomic information from one species to another. However, currently available softwares are relatively difficult to directly use for researchers that are not familiar with use of bioinformatic tools. Therefore, we intended to develop a new platforms and/or interface through which one can use in more comfortable way, based on the concept of interactive comparative analysis. Towards this direction, we, firstly, constructed relational database to store the information on abiotic stress genes identified from multiple plant species using various resources, such as the TAIR (http://www.arabidopsis.org), gene expression profiles and relevant literatures, and linked with comparative analysis interface. For purposes of comparative analysis and identification of synteny blocks, cross-species orthologous genes were determined using a combination of tBlastX and BlastP homology searches. We adapted and developed a Circos-like format to present resulting comparative maps. Users can readily choose analysis parameters, for example individual genes and specific chromosomes for chosen species, in the pane of analysis DB, which is useful feature to avoid complexity of comparative genomic analysis. This DB-associated comparative analysis tool, developed in this study, will be able to provide customer-friendly interface for comparative analysis and extend its utility across a broader range of plant genomes.
        10.
        2014.02 KCI 등재 서비스 종료(열람 제한)
        An electric motor is the one of the most important parts in robot systems, which mainly drives the wheel of mobile robots or the joint of manipulators. According to the requirement of motor performance, the controller type and parameters vary. For the wheel driving motors, a speed tracking controller is used, while a position tracking controller is required for the joint driving motors. Moreover, if the mechanical parameters are changed or a different motor is used, we might have to tune again the controller parameters. However, for the beginners who are not familiar about the controller design, it is hard to design pertinently. In this paper, we develop a nominal robust controller model for the velocity tracking of wheel driving motors and the position tracking of joint driving motors based on the disturbance observer (DOB) which can reject disturbances, modeling errors, and dynamic parameter variations, and propose the methodology for the determining the least control parameters. The proposed control system enables the beginners to easily construct a controller for the newly designed robot system. The purpose of this paper is not to develop a new controller theory, but to increase the user-friendliness. Finally, simulation and experimental verification have performed through the actual wheel and joint driving motors.
        11.
        2013.07 서비스 종료(열람 제한)
        Comparative analysis is a typically useful tool for translating genomic information from one species to another. However, currently available softwares are relatively difficult to directly use for researchers that are not familiar with use of bioinformatic tools. Therefore, we intended to develop a new platforms and/or interface through which one can use in more comfortable way, based on the concept of interactive comparative analysis. Towards this direction, we, firstly, constructed relational database to store the information on abiotic stress genes identified from multiple plant species using various resources, such as the TAIR (http://www.arabidopsis.org), gene expression profiles and relevant literatures, and linked with comparative analysis interface. For purposes of comparative analysis and identification of synteny blocks, cross-species orthologous genes were determined using a combination of tBlastX and BlastP homology searches. We adapted and developed a Circos-like format to present resulting comparative maps. Users can readily choose analysis parameters, for example individual genes and specific chromosomes for chosen species, in the pane of analysis DB, which is useful feature to avoid complexity of comparative genomic analysis. This DB-associated comparative analysis tool, developed in this study, will be able to provide customer-friendly interface for comparative analysis and extend its utility across a broader range of plant genomes.
        12.
        2013.07 서비스 종료(열람 제한)
        Cross-species translation of genomic information may play a crucial role in applying biological knowledge gained from one species to other genomes. To screen and identify a broad range of abiotic stress-responsive genes, we employed a diverse array of resources, including Arabidopsis databases (http://www.arabidopsis.org), expression profiling data and previously reported literatures. As a result, a total of 1,377 genes were identified and classified into 18 different functional criteria based on biological processes of gene ontology. The gene set was translated into M. truncatula, which is a representative model system in the Fabaceae, by identifying orthologous genes between these two genomes with a combination of tBlastx and BlastP analyses. It is shown that approximately 82% of genes were estimated to be translated between the two genomes below the E-value of 10-30. These orthologous loci were used to construct comparative maps by developing a user-friendly analysis platform, resulting in a total of 52 synteny blocks. Furthermore, to discover central genes by which control responses to the abiotic stresses, a combination of AraNet (http://www.functionanet.org) and the Cytoscape program was used for the gene network analysis. The analysis resulted in the identification of 240 potential key genes. We anticipate that these genes may impact molecular breeding programs by discovering trait-associated SNPs followed by marker development.
        13.
        2013.07 서비스 종료(열람 제한)
        The legume family is the third largest group, including approximately 650 genera and 18,000 species, in the flowering plants and the second important crops to the Poaceae in the agricultural economy. Comparative analysis is a useful tool to understand cross-species genomic structure and alterations during organism’s evolutionary history. In this study, we constructed a composite comparative map of ten legume species, including Medicago truncatula, Medicago sativa, Lens culinaris, Pisum sativum, Lotus japonicus, Cicer arietinum, Vicia faba L, Vigna radiata, Phaseolus vulgaris and Glycine max. Of these species, M. truncatula, which is a representative model system, played a central role to develop the cross-genome amplifiable PCR gene markers for the purpose of transferring them to other related legume species. A total of 108 cross-species core markers were employed to analyze genomic colinearity across this broad array of legume species. The comparative map demonstrates a diverse array of evolutionary events, such as duplications, inversions and reciprocal translocations. It is anticipated that resulting maps would provide a broader insights into the lineage-specific genomic organization of these glalegoid/phaseoloid legumes, which are two clades containing almost all crop legumes of economic importance, and can further used for the molecular breeding through translating genomic information into other orphan legumes.
        14.
        2012.11 KCI 등재 서비스 종료(열람 제한)
        In this paper, developing of a pool cleaning robot is addressed. First, we analyze commercial pool cleaning robot mechanisms, and the merits and demerits of wireless version of a pool cleaning robot is introduced. And then the water-jet moving mechanism for a pool cleaning robot is proposed to improve energy efficiency and mechanical design advantage, which is one of the strong candidates for wireless pool cleaning robots. Next, the method of cleaning performance evaluation of pool cleaning robots is firstly defined with five key factors, and it was verified by experimental results. If the cleaning performance can be quantitatively defined, we can design optimally a pool cleaning robot, which results in the cost down.
        15.
        2012.07 서비스 종료(열람 제한)
        For purposes of studying intron structures and predicting consensus splice motifs, a total of 102 legume species were used to isolate introns across the family. Of 196 gene-targeted PCR primer pairs, we successfully amplified 118 intron-containing genes (60.2%) and obtained a total of 1,870 introns with an average size of 143 nucleotides. Species-based compilation of 5’- and 3’-splicing motifs showed lineage-specific conservation in each splicing motif. Compilation of the entire intron set permitted prediction of the consensus sequences of splicing signal motifs in legumes, AYGWGTABABGH and TVNC/TAGGHTV for the 5’- and 3’-splicing motifs, respectively. Interestingly, these consensus motifs are very similar to the corresponding splicing signals of two model systems, Arabidopsis and rice. This result is suggestive of conservation of pre-mRNA splicing mechanisms in higher plants. Multiple alignments of CALTL introns demonstrated that the region from the branch point to 3’ splice site was relatively more conserved than the region from5’ splice site to the branch point. Phylogenetic analysis demonstrated that each of three splicing motifs, 5’-splice sites, 3’-splice sits, and branch site, was relevant to evolutionary divergence of species and phylogenetically informative, suggesting that splice signal sequences would be useful as a potential tool for the molecular phylogenetic analysis.
        16.
        2012.07 서비스 종료(열람 제한)
        A core genetic map of the legume Medicago truncatula has been established by analyzing the segregation of 288 sequence-characterized genetic markers in an F2 population composed of 93 individuals. These molecular markers correspond to 141 ESTs, 80 BAC end sequence-tags, and 67 resistance gene analogs, covering 513 cM. In the case of EST-based markers we used an intron-targeted marker strategy, with primers designed to anneal in conserved exon regions and amplify across intron regions. Polymorphisms were significantly more frequent in intron vs exon regions, thus providing an efficient mechanism to map transcribed genes. Genetic and cytogenetic analysis produced eight well-resolved linkage groups, which have been previously correlated with eight chromosomes by means of FISH with mapped BAC clones. We anticipated that mapping of conserved coding regions would have utility for comparative mapping among legumes; thus 60 of the EST-based primer pairs were designed to amplify orthologous sequences across a range of legume species. As an initial test of this strategy, we used primers designed against M. truncatula exon sequences to rapidly map genes in Medicago sativa. The resulting comparative map, which includes 68 bridging markers, indicates that the two Medicago genomes are highly similar, and establishes the basis for a “Medicago” composite map.
        17.
        2012.07 서비스 종료(열람 제한)
        Cross-species translation of genomic information may play a pivotal role in applying biological knowledge gained from one species to other genomes. Abiotic stress-responsive genes in Arabidopsis have been translated to a legume model system, Medicago truncatula. A total of 1,370 Arabidopsis genes were identified by searching TAIR database, expression profiling data and literatures. For purposes of cross-genome identification of orthologous genes, tBlastX or BlastP were employed between these two model systems. Candidate genes potentially associated with abiotic stress responses were classified into 18 functional criteria and corresponding genomic locations were analyzed by Circos program. To do this, user-friendly bioinformatic analysis platform was established. In order to discover abiotic stress-associated genes, gene network and/or interactome analyses were conducted using a combination of AraNet web-based platform and CytoScape program. As a result, we could identify 240 key genes that appeared to play an important role within central gene networks. We anticipate that these genes may impact molecular breeding programs by developing them into genetic markers and discovering trait-associated nucleotide variations.
        18.
        2007.06 KCI 등재 서비스 종료(열람 제한)
        The thruster is the crucial factor of an underwater vehicle system, because it is the lowest layer in the control loop of the system. In this paper, we propose an accurate and practical thrust modeling for underwater vehicles which considers the effects of ambient flow velocity and angle. In this model, the axial flow velocity of the thruster, which is non-measurable, is represented by ambient flow velocity and propeller shaft velocity. Hence, contrary to previous models, the proposed model is practical since it uses only measurable states. Next, the whole thrust map is divided into three states according to the state of ambient flow and propeller shaft velocity, and one of the borders of the states is defined as Critical Advance Ratio (CAR). This classification explains the physical phenomenon of conventional experimental thrust maps. In addition, the effect of the incoming angle of ambient flow is analyzed, and Critical Incoming Angle (CIA) is also defined to describe the thrust force states. The proposed model is evaluated by comparing experimental data with numerical model simulation data, and it accurately covers overall flow conditions within 2N force error. The comparison results show that the new model's matching performance is significantly better than conventional models'.