Rapid, simple, and sensitive detection of pathogen bacteria is a highly topical research area due to increasingly concerning of food safety and public health. Surface-enhanced Raman spectroscopy (SERS) is a promising and attractive technique offering fast, sensitive, comparatively low-cost, and in-suit detection of pathogenic bacteria. However, this technique requires the preparation step for reducing the noise derived from heterogeneous matrixes of food sample. Immunomagnetic separation (IMS) is widely used technique enabling separation and concentration of the target analyte. It can be used not only laboratory scale but also field diagnosis easily. Here, we synthesized gold-shelled starch magnetic microparticles (GS@SMMPs) for effective separation and concentration of Escherichia coli O157:H7, which were subsequently subjected to SERS integrated with gold-coated 3D-well substrate for bacterial detection in aqueous solution. GS@SMMPs were labelled by Anti-E. coli O157 monoclonal antibody through gold binding protein and staphylococcal protein G (GBP-SPG) fusion protein. In IMS experiment, the immuno-GS@SMMPs showed high capture efficiency over 90% to E. coli O157:H7, which resulted in 10 times decrease in detection limit in PCR assay. Through SERS assay, E. coli O157:H7 concentrated by immuno-GS@SMMPs were successfully detected even at an extremely low concentration of 101 CFU/ml the subjected to SERS. Moreover, by using sandwich method using SERS reporter consisting of GBP-SPG, we found that E. coli O157:H7 were able to be detected by SERS quantitatively through measuring the SERS intensity of GBP-SPG. This novel strategy combining SERS and IMS could be meaningful for extending the application in SERS for in-suit sensitive detection of pathogenic bacteria.

Incremental sheet forming (ISF) is a highly versatile and flexible process for rapid manufacturing of complex sheet metal parts. Compared to conventional sheet forming processes, ISF is of a clear advantage in manufacturing small batch or customized parts. ISF needs die-less machine alone, while conventional sheet forming requires highly expensive facilities like dies, molds, and presses. This equipment takes long time to get preparation for manufacturing. However, ISF does not need the full facilities nor much cost and time. Because of the facts, ISF is continuously being used for small batch or prototyping manufacturing in current industries.However, spring-back induced in the process of incremental forming becomes a critical drawback on precision manufacturing. Since sheet metal, being a raw material for ISF, has property to resilience, spring-back would come in the case.It is the research objective to investigate how geometrical shaping parameters make effect on shape dimensional errors. In order to analyze the spring-back occurred in the process, this study experimented on Al 1015 material in the ISF. The statistical tool employed experimental design with factors. The table of orthogonal arrays of L8 (27) are used to design the experiments and ANOVA method are employed to statistically analyze the collected data. The results of the analysis from this study shows that the type of shape and the slope of bottom are the significant, whereas the shape size, the shape height, and the side angle are not significant factors on dimensional errors. More error incurred on the pyramid than on the circular type in the experiments. The sloped bottom showed higher errors than the flat one.

Incremental sheet metal forming is a manufacturing process to produce thin parts using sheet metals by a series of small incremental deformation. The process rarely needs dedicated dies and molds, thus, preparation time for the process is relatively short as to be compared to conventional metal forming. Spring back in sheet metal working is very common, which causes critical errors in dimensions. Incremental sheet metal forming is not fully investigated yet. Hence, incremental sheet metal forming frequently produces inaccurate parts. This paper proposes a method to minimize dimensional errors to improve shape accuracy of products manufactured by incremental forming. This study conducts experiments using an exclusive incremental forming machine and the material for these experiments are sheets of aluminum AL1015. This research defines a process parameter and selects a few factors for the experiments. The parameters employed in this paper are tool feed rate, tool diameter, step depth, material thickness, forming method, dies applied, and tool path method. In addition, their levels for each factor are determined. The plan of the experiments is designed using orthogonal array L8 (27) which requires minimum number of experiments. Based on the measurements, dimensional errors are collected both on the tool contacted surfaces and on the non-contacted surfaces. The distances between the formed surfaces and the CAD models are scanned and recorded using a commercial software product. These collected data are statistically analyzed and ANOVAs (analysis of variances) are drawn up. From the ANOVAs, this paper concludes that the process parameters of tool diameter, forming depth, and forming method are the significant factors to reduce the errors on the tool contacted surface. On the other hand, the experimental factors of forming method and dies applied are the significant factors on the non-contacted surface. However, the negative forming method always produces better accuracy than the positive forming method.

The usage of corrugated cardboard for packing material is increasing in these days because it is light and easy to manufacture packing boxes. However, the structure analysis of packing boxes, made of cardboard, is not well carried. The reason can be deduced that its mechanical properties for structure analysis are not well known. The cardboards are made different shapes with various types of raw materials that are paper-based compound. In addition, the cardboards are considered to be orthotropic material. This research finds mechanical properties of triple layered cardboard which is composed of outer liner and inner liner. The moduli of elasticity and of shear for liners are found from tension test and T-Peel test. The mechanical properties of the cardboard are calculated using the super position method and equivalent evaluation method.

기계가공은 절삭 부위의 냉각작용과 윤활작용을 위해 절삭유를 많이 소모한다. 절삭유는 염소계의 극압첨가제 등이 함유되어 있어 작업자들에게 유독할 뿐만 아니라 대기의 오염을 초래하여 청정생산을 저해하게 되므로 이런 전통적인 방법은 작업자의 직업병으로부터 보호와 환경보호를 위하여 새로운 가공방법으로 변경되어야만 한다. MQL기계가공 방법은 절삭유를 아주 소량 소모하므로 청정생산을 위한 대안으로 떠오르고 있지만 많은 작업자들이 이에 대한 기술적인 확신이 부족하

  Machining processes produce high accurate metallic parts in metal working industries. Lubrication for machining enhances quality of machined surface and it prolongs the life of cutting tools. Since lubricant is poisonous to human body and environment, i

  재료의 중량과 강도는 기계부품 특히 항공기의 부품에 중요한 요소가 되므로 가볍고 강인한 열처리 강화 알루미늄이나 티타늄 등이 많이 사용된다. 그러나 알루미늄은 용융점이 낮기 때문에 기계 가공 시 발생되는 열에 의해 부품이 얇고 길수록 쉽게 변형된다.   본 연구는 end milling 가공에서 최적의 절삭 parameter를 선정하여 열 변형을 최소화한다. 밀링 가공의 절삭속도, 이송속도, 절삭 깊이를 실험 인자로 정하여 다구찌 방법으로 실험을 계획

Machining processes generate severe heat, which deforms thin metallic parts while they are being processed. The amount of deformation of a thin part is mainly dependent on heat and the shape of a component. The selection of machining parameters can decrease the temperature arisen during a machining process. This paper investigates selecting machining parameters to minimize the heat-deformation that is produced during machining. Taguchi method is applied to setup experiments. Three factors and three levels for each factor in the experimental design are selected. This research analyzes the effects of machining parameters to minimize the heat-deformation through examining the analysis of variance (ANOVA) and the signal to noise ratio (S/N).

High-speed machining is one of the most effective technologies to improve productivity. Because of the high speed and high feed rate, high-speed machining can give great advantages for the machining of dies and molds. High speed machine tool makers try to

High speed machining is a machining process which cuts materials with the fast movement and rotation of a spindle in a machine tool. It reduces machining time because of the high feed and the high speed of a spindle. In addition it gets rid of post proces

In order to polish complicated-shaped inner surfaces of models, a new polishing method with magnetic congelation liquid was invented, we called this name MAGIC polishing tool. This tool is magnetic fluid at high temperature, can be solidified by cooling. The distribution of abrasives in the liquid can be controlled by magnetic field. Therefore we can make a special polishing tool which has well arranged abrasives inside after cooling. In order to know the fundamental polishing properties of this new polishing tool, brass plate was polished. The polishing tool was pressed and rotated against the brass plate in specified polishing time. After many experiments, it was found that this type of polishing tool provided enough removal rate and surface roughness.