1. 본 연구는 한국 재래종 강낭콩 209자원의 phytochemical 및 항산화활성을 평가하였다. 2. 항산화활성은 DPPH, ABTS, FRAP, SOD를 분석하였으며 phytochemical은 kaempferol, myricetin, quercetin, naringenin 함량을 각각 분석하였다. 3. 항산화활성은 강낭콩 자원 간 다양한 분포를 보였으며 DPPH의 경우 62.3~643.9 (IC50), ABTS의 경우 0.28~1.49 mgAAE/g, FRAP의 경우 0.41~5.44 mgAAE/g, SOD의 경우 50.4 ~ 299.8 (IC50)로 나타났다. 4. Relative antioxidant capacity index (RACI)로 강낭콩 자원의 항산화활성을 비교한 결과 IT104587이 가장 높은 항산화활성을 보였으며 IT189598이 가장 낮은 항산화활성을 보였다. 5. 분석된 Phytochemical 중에서 한국 재래종 강낭콩에서는 Kaempferol이 가장 높은 함량을 나타냈다. 6. PCA 분석 결과 209자원은 3개의 그룹으로 나뉘었으며 이중 그룹 III에 속한 46자원의 강낭콩이 낮은 항산화활성 및 phytochemical 함량을 보였다. 7. 본 연구 결과는 한국 재래종 강낭콩의 항산화활성 및 phytochemical 정보를 제공하며 이 정보는 강낭콩 품종 개발을 위한 기초 정보로 사용될 수 있을 것이다.

The aim of this report is to understand the status and management of genetic resources on ICRISAT(International Crops Research Institute for the Semi-Arid Tropics) to promote exchange of germplasm and information. ICRISAT genebank at Patancheru, India has the responsibility to collect, conserve, characterize, document and distribute the genetic resources. Also, ICRISAT has performed the estimation of environment tolerance, disease resistance, and functional compounds of genetic resources. ICRISAT has assembled more than 120,000 accessions of chickpea, groundnut, pigeonpea, pearl millet, sorghum, and six small millets. Since 1974 to 2008, ICRISAT provided 1,350,000 accessions with 144 countries to develop new cultivars. The provision pattern of genetic resources in ICRISAT showed that more than 80 % of each crop by the total holdings resources had been offered and that it is the direction of the ideal conserve and management of genetic resources. To evaluate their genetic resources (chickpea, groundnut, pigeonpea, pearl millet, sorghum, and six small millets), they made descritors for each crop and investigated their germplasms. This report would be meaningful to understand utilization and effect of the germplasms to be held at ICRISAT.

Soft rot caused by Pectobacterium carotovorum subsp. carotovorum is a destructive disease of the fleshy storage tissues of vegetables and ornamentals that occurs worldwide. This study was carried out to find an effective method for evaluating soft rot disease on a massive scale in greenhouse and to screen resistant accession for the disease with lettuce germplasm. Spray method with 5 mL suspension of 109 colony formation unit per milliliter (CFU/mL) of P. carotovorum subsp. carotovorum per plant at the 6 ~ 7 foliage leaf stage was the most effective to evaluate soft rot disease. We evaluated resistance to soft rot using this method for 812 accessions of lettuce germplasm introduced from 52 countries. As the results, withered rates for each germplasm accession ranged from 0 to 100 percent and among 812 accessions of lettuce germplasm investigated, 21 accessions showed very high resistance. Among the 21 accessions, 11 accessions were from Korea, each 2 accessions were from USA and Israel, and each 1 accession was from Afghanistan, Germany, Hungary, Netherlands, Pakistan, and Uzbekistan. Accessions from Israel, Syria, and Afghanistan showed high resistance level. Among very high resistant 21 accessions, 12 were landraces, 6 were cultivars, and 3 were wild relatives. The 21 accessions were consisted of 12 leaf lettuce, 5 romaine type, 3 wild relatives, and 1 butterhead lettuce. Leaf lettuce accessions showed the highest resistance level while crisphead lettuce accessions had the lowest resistance level among the cultivated lettuce types. Wild relatives had greater resistance than landraces and cultivars. These accessions which resistance to soft rot will be used for soft rot resistance breeding program in lettuce.

중앙아시아에서 수집한 꽃피는 마늘 A. longicuspis의 주아와 주아를 도입해서 생산한 1년생 통구를 각각 노지에 재배하여 우리나라에서 주아재배를 통한 종구(통구)생산과 통구재배를 통한 마늘생산 가능성을 검토하였다. 평균 월동율은 주아재배에서 77.3%, 통구재배에서는 100%로서 우리나라의 대표적인 재래종인 단양마늘이나 의성마늘에 비하여 훨씬 높아서꽃피는 마늘의 주아와 통구를 중부지방에서 재배함에 있어서월동성은 큰 문제가 되지 않을 것으로 사료되었다. 주아재배에서 월동한 모든 개체가 통구 수확이 가능하였고 통구 형성율은 모든 자원이 100%이었다. 통구재배에서 추대율이 100%이었으며, 재래종 대비 화경장이 길고 주아 수가 많았다. 구크기는 대부분이 재래종에 비하여 작았지만 K248215 자원은구중이 40 g으로서 단양마늘과 비슷하였으며 구당 쪽수는 재래종에 비하여 대부분의 자원이 많았고 화기에 꽃과 주아가동시에 존재하였으며 주아를 제거하지 않은 상태에서는 종자가 맺히지 않았다.

1. 카자흐스탄은 중앙아시아의 북쪽에 동서로 길게 위치한 국가로서 러시아, 중국, 우즈베키스탄 등 5개국과 국경을 접하고 있고 사막과 스텝이 국토면적의 80%이상을 차지하며, 동남부 지역은 4,000 m 이상의 산악지대 이고 국토의 최저점과 최고점의 차이가 7,000 m 이상이다. 2. 카자흐스탄은 전통적인 농업국가 이었지만 최근 석유산업의 발달로 농업생산이 GDP에 기여하는 비율이 5.2%로 낮아졌으나 경제 활동인구 중 농업종사자 비율이 25.9%로 높은편이다. 3. 카자흐스탄은 경지면적이 24,035천 ha로 넓지만 농업생산이 밀 위주로 되어 있어서 밀 생산량이 세계 10위, 수출은 7위이나 과일, 채소 및 유료작물은 상당량 수입하고 있으며, 강우량이 적고 건조한 기후조건 때문에 주로 관개농업에 의존하고 있다. 주요 농작물은 밀, 보리, 채소, 사료작물, 목화, 옥수수, 벼, 감자, 과수, 유료작물이다. 4. 축산업이 농업전체 생산액에서 차지하는 비율은 38.9%로서 높은 편이나 많은 량의 닭고기, 돼지고기, 우유 및 계란을 수입하고 있으며 유우, 육우, 면양, 돼지, 닭 및 말이 주된 가축이다. 5. 카자흐스탄은 야생 양파와 마늘 등을 포함한 400여 작물의 선조종이 자생하며 사과·살구 등 6 과수 종의 원산지이고 종자은행에는 75,000점의 자원을 수집하여 현지외 보존하고 있는 자원부국이다. 6. 현재 카자흐스탄과는 농업기술협력이 미미한 실정이나 카자흐스탄과의 현 실정으로 볼 때 농업기술 협력은 자급이 되지 않아 해외에서 수입하고 있는 작물의 재배나 생산성향상기술, 관개방법, 채소재배 시설 확충, 농기계, 농산물 가공분야, 양계 및 유전자원 교환 등이 협력 사업으로서 유망할 것으로 사료된다.

종실 돌연변이를 유기하여 미질개선을 위한 육종으로의 적극적인 활용을 목적으로 얻어진 신동진벼 돌연변이 계통의 작물학적 특성과 종실 저장단백질을 변이모본과 비교 분석을 검토하여 얻은 결과를 요약하면 다음과 같다. 1. 종실 변이계통의 작물학적 특성은 변이모본보다 생태특성의 출수기에서는 대부분 조생의 경향을 보였고, 간장 및 수장은 짧은 경향을 나타냈고, 또한 종실특성의 현미 길이, 현미폭 및 천립중에서도 변이모본보다 짧거나 낮은 정도를 나타냈다. 2. SDS-PAGE 분석결과 opaque 군의 SM-22와 giant embryo 군의 SM-34는 글루테린 폴리펩타이드에서 높은 농도를 보였고, floury 군의 SM-23, shrunken 군의 SM-26, sugary 군의 SM-31은 전단백질 농도패턴은 낮게 보이면서 55kDa 이상의 고분자 band에서 다양성을 나타냈다.

China is a one of the largest agricultural countries in the world. China consumes around 12.5 billion kilograms of seeds each year. Suchhuge demand for seeds has made the Chinese seed market more and more attractive for investment. Through analysis on the present situation and existing problems of the seed industry in China and based on the current Chinese seed industry development, some future prospects for investments are indicated. This investigation was carried out to propose the appropriate strategies on the development of the Korea seed industry as it considers its entry into the China seed market as a new growth engine in the agricultural sector. The basic law regulating the Chinese seed industry is the PRC Seed Law that generally refers to the protection of germplasm resources, verification of varieties, seed quality issues, the import and export of seeds, seed administrative management, and various rights and obligations. The regulations were aimed at the protection of the rights concerning new varieties of plants. China has two main industry associations, the National Seed Association and the China Seed Industry IP Union, that are non-profit associations consisting of entities and people engagin in the seed scientific research, production, operation and management. The China National Seed Group Co., Ltd. (“Sino Seeds”) is the market leader in China regarding the seed industry. The chinese government, however, encourages investment from multinational companies as well as importation of modern crop planting management technologies and equipment. It supports the entry of investors with proven experiences in breeding and germplasm resources expansion and R&D. There has never been a better time for multinational companies with proven seed industry experience to look at building relationships with the Chinese government and enterprises.

This study describes an efficient and stable droplet vitrification following cryopreservation of strawberry shoot tip (Fragaria × ananassa Duch.) accessions ‘Massey’ and ‘MDUS3816’. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 17.5% glycerol and 17.5% sucrose for 40 min and exposed to dehydration solution (B1) containing 50% glycerol and 50% sucrose for 40 min at 25oC. Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 ㎝× 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with 0.3M sucrose for 30 h + 0.5M sucrose for 16 h at 25oC. The cryopreserved shoots tips exhibited 57.8 % recovery rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks. Variation was not observed in both of ploidy analysis and morphological investigation on plantlets of two accessions cryopreserved under variable preculture conditions.

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of strawberry (Fragaria × ananassa Duch.) cvs. ‘Wonkyo3114’ and ‘Gurumi40’. The shoot tips were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.5M). Precultured explants were osmoprotected with loading solution (LS, C4) containing 20% glycerol and 20% sucrose for 40 min and exposed to dehydration solution (B5) containing 40% glycerol and 40% sucrose for 40 min at 25℃, Subsequently, the explants were transferred onto droplets containing 2.5 μL PVS3 on sterilized aluminum foils (4 ㎝ × 0.5 ㎝) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regrowth rate (%) in both the cultivars was obtained when the shoot tips were precultured with MS + 0.3M sucrose for 40 h at 25℃. The cryopreserved shoots tips exhibited 55% regrowth rate by culturing in NH4NO3-free MS medium supplemented with 3% sucrose, 1.0 g/L casein, 1.0㎎/L GA3, and 0.5 ㎎/L BA for 5 weeks and in MS medium supplemented with 0.5 ㎎/L GA3 for 8 weeks. This result shows that droplet-vitrification could be employed as a promising method for cryostorage of strawberry germplasm.

The adzuki bean (Vigna angularis L.) is a red-grained legume that has a number of essential nutrients and is used in traditional dishes in Asia. Adzuki bean industrial by-products are also a potential low-cost source of some unique bioactive polyphenols. Hence, here, the authors aimed to perform a comparative study of the phytochemical profiles of the leaves and seeds of the adzuki bean and compare their antioxidant, α-glucosidase inhibition, and tyrosinase inhibition activity. The authors assessed antioxidant activity by DPPH, ABTS, FRAP, PR, TPC, and SOD assays, which showed wide variation, respectively. From the relative antioxidant capacity index results, 10 adzuki bean landraces were selected to compare for phytochemicals and bioactivity using leaf and seed extracts. Antioxidant, α-glucosidase inhibition, and tyrosinase inhibition activity in the leaf extracts were higher than in the seed extracts, and there were more flavonols and isoflavones in the leaf extracts than in the seed extracts. This study demonstrated that adzuki bean leaf extracts could be a new natural antioxidant or antidiabetic agent and a skin whitener and can also be used in industrial applications.

Eleutherococcus senticosus (Siberian ginseng) is an important medicinal tree found in northeast Asia. In this study, we analyzed the genome-wide distribution of microsatellites in E. senticosus. By sequencing 711 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in E. senticosus accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 131 E. senticosus accessions in Korea and China. The number of alleles ranged from 2 to 11, with an average of 7.4 alleles. The mean values of observed heterozygosity (HO) and expected heterozygosity (HE) were 0.59 and 0.56, respectively. The average polymorphism information content (PIC) was 0.51 in all 131 E. senticosus accessions. E. senticosus accessions in Korea and China showed a close genetic similarity. Significantly low pairwise genetic divergence was observed between the two regions, suggesting a relatively narrow level of genetic basis among E. senticosus accessions. Our results not only provide molecular tools for genetic studies in E. senticosus but are also helpful for conservation and E. senticosus breeding programs.

Garlic (Allium sativum L.) has been used as both food and medicine in many cultures for thousands of years. Garlic cultivars are completely sterile and propagated through vegetative method. Collection of a large number of fertile accessions of these genus is needed to explore genetic variability. In order to investigate genetic variation among Allium species and its possibilities for direct cultivation in Korea, we characterized 12 accessions of A. longicuspis, flowering wild garlic which had collected from Central Asia, the main center of garlic diversity. Most of A. longicuspis accessions showed higher over-wintering and bolting rate, longer scape length and more number of bulbils than Korean landraces cultivar, Danyang and Euiseong, but A. longicuspis accessions exhibited smaller size of bulbs and bulbils. Most accessions of A. longicuspis had more number of cloves per bulb, except K229596 and K248824 than Korean landraces. All the accessions of A. longicuspis from Central Asia had complete bolters having many flowers and topsets in umbel. Further studies of A. longicuspis should focus on securing true seeds through removal of topsets and crosses among accessions to create the genetic variability.

The chloroplast (cp) is an organelle with its own genome encoding a number of cp-specific components. The membrane-bound organelles are mainly involved in the photosynthetic conversion of atmospheric CO2 into carbohydrates in which light energy is stored as chemical energy. Resequencing technology via next-generation sequencing has recently been successfully applied which results the field of cp genome characterization is growing fast. Here, we report the complete sequence of the chloroplast genome of Capsicum frutescens, a species of chili pepper. The total length of the genome is 156,817 bp, and the overall GC content is 37.7%. A pair of 51,584-bp inverted repeats (IRs) is separated by a small (17,853 bp) and a large (87,380 bp) single-copy region. The C. frutescens chloroplast genome encodes 103 unique genes, including 79 protein-coding genes, 20 tRNA genes, and four rRNA genes. Of these, 19 genes are duplicated in the IRs and 18 genes contain one or two introns. Comparative analysis with reference cp genome revealed 125 simple sequence repeat (SSR) motif and 34 variants, mostly located in the non-coding regions. These microsatellite markers will facilitate the studies of genetic diversity, population genetic structure, and sustainable conservation for C. frutescens.

Chloroplast (cp) genome sequences provide a valuable source for DNA barcoding. Molecular phylogenetic studies have concentrated on DNA sequencing of conserved gene loci. However, this approach is time consuming and more difficult to implement when gene organization differs among species. Here we report the complete re-sequencing of the cp genome of Capsicum pepper (Capsicum annuum var. glabriusculum) using the Illumina platform. The total length of the cp genome is 156,817 bp with a 37.7% overall GC content. A pair of inverted repeats (IRs) of 50,284 bp were separated by a small single copy (SSC; 18,948 bp) and a large single copy (LSC; 87,446 bp). The number of cp genes in C. annuum var. glabriusculum is the same as that in other Capsicum species. Variations in the lengths of LSC, SSC and IR regions were the main contributors to the size variation in the cp genome of this species. A total of 125 simple sequence repeat (SSR) and 48 insertions or deletions variants were found by sequence alignment of Capsicum cp genome. These findings provide a foundation for further investigation of cp genome evolution in Capsicum and other higher plants.

Chloroplast DNA sequences are a versatile tool for species identification and phylogenetic reconstruction of land plants. Different chloroplast loci have been utilized for phylogenetic classification of plant species. However, there is no evidence for a short sequence that can distinguish all plant species from each other. Molecular markers derived from the complete chloroplast genome can provide effective tools for species identification and phylogenetic resolution. Thus, the complete chloroplast genome sequence of Korean landrace “Subicho” pepper (Capsicum annuum var. annuum) has been determined here. The total length of the chloroplast genome is 156,878 bp, with 37.7% overall GC content. A pair of IRs (inverted repeats) of 25,801 bp was separated by a small single copy (SSC) region of 17,929 bp and a large single copy (LSC) region of 87,347 bp. The chloroplast genome harbors 132 known genes, including 87 protein-coding genes, 8 ribosomal RNA genes, and 37 tRNA genes. A total of seven of these genes are duplicated in the inverted repeat regions, nine genes and six tRNA genes contain one intron, while two genes and a ycf have two introns. Analysis revealed 144 simple sequence repeat (SSR) loci and 96 variants, mostly located in the non-coding regions. The types and abundances of repeat units in Capsicum species were relatively conserved and these loci will be useful for developing molecular markers.