OBJECTIVES : In this study, microstructural components of crumb rubber modified asphalt (CRMA) binder were investigated using environmental scanning electron microscope (ESEM). To clearly understand the elemental composition of the CRMA binder, energy dispersive X-ray spectroscopy (EDX) was employed on the ESEM samples. METHODS: CRMA binders were produced using open blade mixers at 177℃ for 30 min. The binders were artificially aged through a series of accelerated aging processes. Sample preparation was done by making a mold shape on the glass slide. Thereafter, the morphology of the CRMA binder was observed using the ESEM coupled with the EDX. RESULTS : The images captured from the ESEM indicate that the unaged CRMA binder appears to have a single-phase continuous nonuniform structure after the addition of crumb rubber particles, whereas the artificially aged CRMA binder was observed to have two different phases. ESEM coupled with EDX shows detailed internal structure of the modified binders compared to other technologies (i.e., optical microscopy, atomic force microscopy, and conventional scanning electron microscope). CONCLUSIONS: The captured images resemble the internal structures such as the viscous properties of the unaged CRMA binder and the interaction between the rubber particles and the base binder at aged condition. ESEM is a powerful instrument and with the introduction of EDX, it provided more details of the network microstructure of the asphalt binder. ESEM coupled with EDX is recommended for use in future investigation of microstructure of asphalt binders.

PURPOSES : The performance properties (indirect tensile strength, rutting resistance, and resilient modulus) of recycled aged CRM mixtures and their correlation with Superpave binder properties (viscosity, high failure temperature, G*sinδ, and stiffness) were investigated. METHODS: A series of Superpave binder tests was performed by using a rotational viscometer, DSR, and BBR to evaluate the performance properties. In addition, the CRM mixes were artificially aged through accelerated aging processes, and their properties were evaluated. The correlation between the properties of recycled aged CRM binders and the engineering properties of recycled aged CRM mixtures was experimentally determined. RESULTS : The rut depth values decreased and the ITS values increased with increasing high failure temperature. In general, the resilient modulus properties seemed to be poorly correlated with the high-temperature values, regardless of the aggregate source. CONCLUSIONS: The recycled aged CRM binders and mixtures can lead to satisfactory performance, and the properties of these binders are strongly correlated with the engineering properties of the mixtures.

PURPOSES : This paper presents a description of the current issues facing road managers regarding the surface-type conversion of lowvolume roads for cost savings. METHODS: The paper reviews previous works conducted toward this end, acknowledges gaps in the current research, and lays out what information is needed for further studies. RESULTS : If the cost to maintain an unsurfaced road is less than the cost of maintaining a surfaced road, then there is potential for cost savings for the management agency. However, the problem is bigger than simply maintaining the roads that already exist. If unsurfaced roads prove to be more economical than surfaced roads, then the cost to convert from a surfaced to an unsurfaced roadway, and vice versa, when necessary, must also be examined. CONCLUSIONS : No other studies have addressed the un-surfacing of a road for cost savings, and it is therefore unknown whether substantial savings can be realistically obtained by converting from a surfaced to an unsurfaced road. To determine whether a conversion policy would be a viable option, additional data and research are needed.

PURPOSES: This study set out to investigate the current state of microsurfacing in Texas and compared the results with the current state of the practice nationwide. METHODS: For this study, case studies were extracted from the existing literature and compared with the data obtained both from site visits by the research team and data obtained from a survey by Texas Department of Transportation (TxDOT) personnel. The successes and failures of microsurfacing are detailed and explanations of different issues are outlined. Forensic studies from Texas are included in the descriptions of those microsurfacings that failed when configured as part of the cape seals. RESULTS : Microsurfacing has been shown to be an effective pavement preservation technique when applied to an appropriate road, at an appropriate timing, and as a remedy for certain issues. The failures experienced in Texas can mostly be attributed to cape seals and an inability to recognize structurally faulty pavement. CONCLUSIONS : When applied to an appropriate road, at an appropriate timing, and as a remedy for certain issues, microsurfacing has been shown to perform well in numerous case studies. The majority of microsurfacing failures are the result of poor project selection, usually involving the treatment being applied to structurally unsound pavement.

2013년 11월부터 2014년 7월까지 가축유전자원시험장 장내에서 사육 중인 한우 및 칡소 206두(한우 187두, 칡소 19두)를 대상으로 연령별, 종별 그리고 성별로 각각 분류하고, 이들에 대한 혈액학적 수치를 확인하였다. 혈액학적 검사 결과에서 1년 이하에서 5년 이상으로 연령이 증가됨에 따라 RBC(12.0~9.5x106/μl로)와 PLT치(717~432x103/μl로)에서 각각 유의성 있는 감소(P＜0.05)가 인정되었다. 동일 연령(2~3년)의 한우군과 칡소군 간에서의 비교에서는 RBC(10.1~10.4x106/μl)와 HCT(44.8~42.1%), MCV(49.4~44.9 fl) 및 MCHC (28.9~31.1 g/dl)에서 두 품종 간의 차이점이 인정되었으며, MCV와 MCHC에서도 통계적 유의성이 인정되었다(P＜0.05). 또한, 백혈구계 검사 결과에서도 연령이 증가함에 따라 WBC 수치(1년 이하의 13.8에서 5년 이상의 9.5x103/μl로)가 감소하는 경향이 인정되었으며, 백혈구 분포의 백분율에서는 전반적으로 림프구가 46.2%를, 분엽형 호중구가 35.6%로 나타났다. 한우군과 칡소군 간의 백혈구계 비교에서는 칡소군의 분엽형 호중구치가 2.7x103/μl로서 한우군에 비하여 유의성 있는 저하(P＜0.05)가 인정되었으며, 림프구치는 5.2x103/μl로 4.7x103/μl 한우군에 비하여 높게 나타났다. 임신우군과 비임신우군 간의 비교 분석에서는 Hg(9.9 g/dl)과 HCT(40.5%)이 비임신군에 비하여 통계적 유의성이 있는 저하(P＜0.05)가 인정되었으며, 총 백혈구수와 분엽형 호중구수, 림프구수 모두 비임신우군에 비하여 높게 나타났지만, 통계적 유의성은 인정되지 않았다.

Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts (10.2x1014/mol s-1 versus 6.4x1014/mol s-1, p＜0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over 12.0x1014/mol s-1, respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

본 연구에서 생쥐 초기 배아를 이용하여 동결 보존된 배아의 생존율 또는 발달율에 영향을 미치는 요인들의 상관관계를 알아본 결과, 배아의 발달 단계에 따른 동결 - 해동 후 생존율에 있어서는 2세포기 배아가 4~8세포기 배아보다 유의하게 높았으나(p＜0.01), 배 발달율에 있어서는 4~8세포기 배아가 유의하게(p＜0.01), 높아 생존율과 배 발달율과는 상관관계가 없는 것으로 사료된다. 또한, 동결 보호제에 따른 배아의 생존율에 있어서는 2, 4~8세포기 배아 모두 DMSO에서 유의하게 높았으나(p＜0.01), 배 발달율에 있어서는 EG가 DMSO에 비해 더 유의하게 높은 성적을 나타내어(p＜0.01, p＜0.05) 동해로 인한 상해가 적은 것으로 생각되어 2, 4~8세포기 배아에서 EG가 더 효과적인 동결 보호제로 사료되며, 동결 프로그램으로는 완만 동결 - 급속 해동법이 더 우수한 프로그램으로 보인다.

생쥐 2세포기, 4세포기, 8세포기를 각 발생 단계에서 채취하여 동결 보호제를 첨가한 서로 다른 배양액에서 배양하고, 배아의 동결 보존 및 융해 시 급속 처리와 저속 처리 단계로 비교하여 이들 조건이 배아의 생존과 발현에 미치는 영향을 조사하여 다음의 결과를 얻었다. 동결 보호제로 처리하여 배양액을 달리한 경우, 급속 단계에서는 모든 배양액에서 비슷한 발생율을 보였고, 저속단계의 4세포기와 8세포기는 D-PBS에서 높은 발생율을 보였다(P＜0.05, P＜0.01). 배아의 발생 시기에 따른 동결 보존 후, 발생율은 2, 4, 8세포기로 넘어갈수록 발생율의 증가를 보여 8세포기에서 발생율이 가장 높았다(P＜0.01). 동결 보호제의 처리단계에 따른 발생율은 2세포기의 급속 단계에서는 유사하였으나, 4세포기와 8세포기는 저속단계에서 높은 발생율을 보였으며(P＜0.05), 특히 8세포기에서 가장 높았다(P＜0.01).

Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vitro models. RT-PCR and real time PCR analysis respectively revealed that LH had no effect on c-Ski mRNA expression in the cultured granulosa cells regardless of LH treatment. Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-Ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally. Moreover, expression of mRNA of Arkadia, an E3 ubiquitin ligases, in luteinizing granulosa cells in vivo was assessed by realtime-PCR. The levels of Arkadia mRNA expression were unchanged during follicular growth and postovulatory luteinization. These findings suggest that Ski protein level may be regulated during luteinization at translational and/or post-translational level but not by Arkadia.

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski protein is implicated in proliferation/differentiation in a variety of cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of this study was, by means of immunohistochemical methods, to locate Ski protein in the rat ovaries during ovulation and corpora lutea (CL) formation to predict the possible involvement of Ski in luteinization. In addition, we performed to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vivo models. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). These results indicate that Ski is profoundly expressed in the luteinized granulosa cells and luteal cells of CL during luteinization, and suggest that Ski may play a role in luteinization of granulosa cells.

소비 시장이 감성 소비 환경으로 바뀌면서 브랜드의 역할이 더욱 중요해졌고, 이를 위한 브랜드 이미지 전략으로서 디자인의 역할이 확장되고 있다. 브랜드와 소비자가 소통하는 지점인 소비자 접점은 체험을 통한 브랜드 이미지가 형성되는 경로이므로, 기업이 지향하는 이미지로 각인시키기 위해서는 소비자 접점에 대한 총체적인 기획 및 관리가 필요하게 되었다. 위와 같은 배경에서, 이 연구의 목적은 변화된 시장 환경에서 성공적인 브랜드 이미지를 구축하기 위한 통합 디자인의 구성 방법을 정의하는 것이다. 이를 위해 브랜드 이미지의 특성, 소비자 체험의 조건, 감성의 역할을 이해하고, 이미지와 감성 처리 모형과의 상관성을 해석하여, 이미지의 3가지 특성을 도출하였다. 이미지의 특성은 1) 경험적 판단에 근거하며, 2) 감성적 내용을 표현 전달하고, 3)관념적 공감에 의한 심리적 가치를 만드는 것이다. 이러한 이미지를 창출하는 디자인의 조건을 성공적인 브랜드의 사례연구를 통해 분석하여, 강력한 이미지를 구축하기 위한 브랜드 아이콘의 역할과 이를 위한 통합 디자인의 필요성을 밝혔다. 이 통합 디자인을 구성하는 방법은 1) 브랜드 이미지 포지셔닝에 의한 이미지 집중화, 2) 브랜드 이미지 플롯에 의한 시각적 주제 활용, 3) 이미지 통합 관리를 위한 Total Identity Program, 4) 브랜드 이미지각인을 위한 Brand Icon 개발 등이다.

Five species of graptolites were discovered from the Jigunsan Shale of Taebaeg area, Korea. They are herein described as Archiclimacograptus riddellensis (Harris, 1924), Pseudamplexograptus distichus(Eichwald, 1840), Hustedograptus teretiusculus(Hisinger) sensu Jaanusson, 1960, Hustedograptus vikarbyensis (Jaanusson, 1960), and Hustedograptus sp. The graptolite assemblage from the Jigunsan Shale corresponds to those of the Pseudamplexograptus distichus zone in the Baltic and German areas and the geologic age of graptolites bearing beds of the Jigunsan Shale might be late Middle Llanvirn.

his Study attempted to comprehend difference between hiker and non-hiker's sense of place value at Namhansanseong Provincial Park. Especially, it was analyzed after visitors, who were divided into local residents & tourists and first time visitors & repeat visitors. Out of 600 visitors, who were over 19 years old, participated in the survey, 587 responses were used for data analysis. Consequently, Sense of place value at Namhansanseong Provincial Park were classified into three factors: Health·Culture, Ecology·Environment, and Connectedness. Hikers feel higher value on promotion of health, relaxation, environment than non-hikers. Among hikers, local residents feel higher value on ecology·environment and connectedness than tourists. Among non-hikers, however, they have no difference between local residents and tourists, only repeat visitors and first time visitors. On this result, possibility of resident participation management on natural environment and trail was considered, and when the program, which is for raising cultural and traditional value, would develop, increasing number of repeat visitor should be considered as a high priority.

The impacts of climate change and land use change are still causing changes in natural patterns as increase of temperature, rainfall intensity, and frequency of rainfall. Also, urbanization is well known to impact hydrology of basins because of its creation of impervious surfaces. This study has been investigated developing method being able to estimate changes of hydrological properties and assessing these changes on each city. For this study, it used three land use scenarios in 1975, 1990, and 2009 for considering impervious changes and future climate change scenario based on RCP 8.5 for impacts of climate change. Three clusters by k-means clustering method were formed by variables shown high correlations from results of Spearman’s order-rank method and Kendall Tau for various variables as rate of each land cover over area of each subwatershed on 145 subwatersheds in Nakdong River, difference of rate of land cover area and CN (Curve Number) between scenarios based on scenario in 1975, and rate of hydrological properties like rate of evaporation, surface runoff, and groundwater. An assessment method using the results from correlation and cluster analysis was developed for deriving the weighted index in accordance with sensitive degree of hydrological property and increase of impervious area. The assessment method for sensitive city applied principal component analysis (PCA) method. Results show that Daegu is greatly sensitive on impacts of hydrological property and increase of impervious area. Consequently, linear regression equations on Daegu are derived by analysis of hydrologic response unit. Urbanization appeared to increase of surface runoff and decrease of evaporation and groundwater in accordance with change of land uses, also the results are considerably important and useful in case of development and design of city.

A cut slope mudstone formed due to construction work has usually been accompanied such as a joint and fracture zone so it especially is required to be significant care when cutting rock construction. Therefore, in this study was analyzed about engineering characteristics through case of a slope activity. In addition, two-dimensional finite element analysis with construction stages and actual measurement results were compared by each displacements. Through this, application between finite element analysis and actual field condition was investigated for stable slope design and construction.

새로운 베지클인 glyceryl citrate/ lactate/ linoleate/ oleate를 이용한 수중유형 형태의 아스타잔틴 나노에멀젼에 대해 항산화 효과, 세포 생존력, 단백질과 관련한 효소의 영향, 피부 침투도 그리고 피부에 대한 보습 및 탄력 등의 약용화장품적인 측면에서의 전반적 연구를 실시하였다. 항산화력 및 세포 생존력에 대해선 각각 DPPH법과 MMT assay를 이용하여 측정하였다. 아스타잔틴 나노에멀젼에 대한 또 다른 성질은 2D-Page를 이용한 단백질 분석 및 컨포칼, in-vivo 테스트를 통해 측정하였다. 본 연구를 통해, 아스타잔틴을 포함하는 나노에멀젼은 MMP발현에 관련한 단백질 억제 및 세포외 기질의 분해를 막고 라디칼의 소거에 매우 우수한 결과를 보였다. 종전의 레시친을 이용한 나노에멀젼 보다는 새로운 베지클을 이용한 아스타잔틴 나노에멀젼의 피부 침투가 매우 효과적임을 CLSM을 통해 측정하였다. 또한 28일 동안의 한국 성인 여성 11명을 통한 보습 및 탄력 인비보 테스트에서 우수한 효과를 확인할 수 있었다.

Space-borne remote sensing is an effective and inexpensive way to identify crop fields and detect the crop condition. We examined the multi-temporal spectral characteristics of rice fields in South Korea to detect their phenological development and condition. These rice fields are compact, small-scale parcels of land. For the analysis, moderate resolution imaging spectroradiometer (MODIS) and RapidEye images acquired in 2011 were used. The annual spectral tendencies of different crop types could be detected using MODIS data because of its high temporal resolution, despite its relatively low spatial resolution. A comparison between MODIS and RapidEye showed that the spectral characteristics changed with the spatial resolution. The vegetation index (VI) derived from MODIS revealed more moderate values among different land-cover types than the index derived from RapidEye. Additionally, an analysis of various VIs using RapidEye satellite data showed that the VI adopting the red edge band reflected crop conditions better than the traditionally used normalized difference VI.

The objective of this research was to analyze the determinants of demand for residential settlements in rural societies. A significant aspect of the demand analysis was to consider depopulation classification as a moderating variable with a view to its role as an essential dividing factor of socioeconomic characteristics and physical environments of the areas of concern. The data collection for analysis was divided according to types of depopulation into the three categories of less developed, stagnated, and developed areas. For the cause and effect analysis between the residential demand and factors of settlement, the ordered probit model was applied. Significant determinants of settlement demand unfolded according to depopulation types. In the case of less developed areas, residential demand was affected significantly by the factors of daily life convenience and public facilities. Key settlement demand determinants of stagnated regions included the aspects of basic natural environment, daily life convenience and education. Meanwhile, key settlement demand determinants for developed areas included education and agriculture economic aspects. The importance-performance analysis was also applied to a set of settlement characteristics of rural communities to figure out the settlement factors requiring urgent endeavor to improve.

Taekwondo, Korean traditional martial art as one of the ten Korean cultural symbols, has acquired a reputation for introducing and globalizing Korean culture together with its standing as official Olympic Game in 2000, Sydney, Australia. Nevertheless the World Taekwondo Headquarters, Kukkiwon, which is an exclusive Taekwondo institution only accommodates for three thousand persons. Considering the worldwide reputation of Taekwondo, the scale of Kukkiwon leaves a big supply problem. This study therefore suggests the plan to design Taekwondo park that playa major role of Taekwondo development in the 21st century and represent the cultural heartland of Taekwondo. The study considers four points. The points are as follows: 1) Necessary programs drawn by analyzing and assessing Muju city as a site for Taekwondo park, then a survey conducted for potential visitors to a Taekwondo park. Under the theme of "A World Cultural Heritage Upon Our Own Lives", the park plan intends to become a shrine of Taekwondo as well as a big tourist attraction. 2) Programs are appropriate for the terms of the investment under the auspices of government, by considering potential visits and the site conditions. 3) The meaning of Taekwondo and its history should be reflected in the space distribution and facilities allotment. Three meaningful zones: symbolic zone, training zone and culture zone are considered in planning program and space distribution. 4) The plan also intends to harmonize with local development by groping Muju city's uplifting, not only designs a Taekwondo park.

Tourism and recreation spots in Korea have been developed metropolitan cities-oriented that facility construction has too much importance in local tourist site development more than satisfaction and experience. Tourism hardly seems to play its role as a motive power even in locals where tourism occupies much in their economic development. Therefore, the ministry of culture & tourism has introduced a plan to discover cultural and tourism resources as a development alternative which handles theme-ability and specialization. However, most projects of local tourism resources developed since 1999 have resulted similar features comparing to previous and existing tourist spots. And the main objectives of this paper have not been realized very well. This research hence forth suggests a program-based model development in tourism resources, with a case study of Gokun-gugok, one of the historical and cultural sites and is located in Hwachon-gun, Gangwon Province. Main points include: Since the Gokun-gugok landscape has been undermined and been loosed the traditional cultural value due to the road development, this study intends to plan to make the adventure of tourism destination including restoring the site as a cultural place. The Gokun-gugok site needs to develop combining various types of tours and adventures with instructive and educational programs to meet the visitors' needs. This research also intends to precede a development plan based on harmonizing natural, historical and cultural assets of the Gokun-gugok with facility maintains and tourism development. Meanwhile, the study stresses on realizing development of tourism resources categorizing programs by seasonality, visitor's economic class, and visit duration. Asa consequence, the research presents a "Culture & Tourism Academy" which deals with these types of adventure programs and informative educations. To assess feasibility of the development plan in terms of economy, environment and policy, the research conducted the site inspection and examined the site's surroundings, land properties and inundation. 145,000 square meters have been extracted as a feasible development area out of total 392,500 square meters. Finally, the study segmentizes target markets basedon the result of the survey on visitors and local residents. The more segmentized markets employ facilities according to their traditional characters.