Background : Cornus kousa fruit has been used as a medicine for a long time. Therefore, for the purpose of utilizing C. kousa fruit as a health functional food, antioxidant activity was tested by extracting ethanol concentrations to identify optimal extraction conditions. Methods and Results : C. kousa fruits used in this study were washed, dried and pulverized. Then, the samples and solvents by ethanol concentration (0, 20, 40, 60 80 and 100%) were extracted at 80℃ for 90 minutes at 1 : 20 ratio, respectively. Afterwards, it was filtered, concentrated, frozen and dried. Total phenol and flavonoid content, DPPH and ABTS radical scavenging activity, ferric reducing antioxidant power and oxygen radical absorbance capacity were measured. The total phenol and flavonoid content is the highest in 60% ethanol extract at 20.08 ㎎·GAE/g, 0.86 ㎎·QE/g. In addition to all antioxidant studies showed the highest activitiy in 60% ethanol extract. Also, when HepG2 cells were treated with ethanol extract at 200 ㎍/㎖ concentrations, the survival rate was more than 90% and cytotoxicity was not observed. Then, HepG2 cells were treated with tBHP to induce oxidative stress, and the cytoprotective effect and inhibitory activity of ROS production were measured. As a result, 60% ethanol extract showed the best activity. Conclusion : When extracting C. kousa fruits with ethanol as solvent, It is expected best to use 60% ethanol to maximize potential as a material for health functional foods.

Background : The Dolwoe is a native plant to Asia and a medicinal plant belonging to the family of cucurbitaceae. It is generally consumed in the form of tea. The purpose of this study was to investigate the effects of roasting treatment and the extracts of ethanol and water on antioxidant activity and to examine the possibility of raw materials for health functional food. Methods and Results : In this study, each specimen was extracted as water and ethanol by dividing it into Dolwoe leaves that were treated with roasting treatment, or hot air drying. To compare each extract, the total phenol and flavonoids content were measured, and the TEAC experimental method was conducted in which ABTS radical was indexed to trolox to measure antioxidant activity. Also, The degree of reduction of iron ions was measured using the FRAP experimental method to compare the reducing power of each extract. As a result of the experiment, the total phenol content was between 35.54 and 71.52 ㎎·GAE, the ethanol extract of roasted leaves showed more than twice the amount of phenol than the ethanol extract of dried leaves. The total flavonoids content was 5.37 to 28.91 ㎎·QE/g, with roasted leaves ethanol extracts with high total phenol content. In particular, antioxidant activity with TEAC 153.90 ± 1.72 mM·TE/g, FRAP 320.78 ± 1.44 mM·FE/g in ethanol extract of roasted leaves showed high activity in proportion to the total phenol and flavonoids content and hydroxyl radical scavenging was found to be the highest in the ethanol extract of roasted leaves. Conclusion : Total phenol and flavonoid content, antioxidant effects were highest when the Dolwoe leaves were processed for roasting treatment and extracted as ethanol. Therefore, extraction under the following conditions will have a useful effect as a health functional food.

Background : Mountain ginseng adventitious roots are being produced in large quantities by using plant tissue cultivation techniques to overcome the disadvantages of recent cultivation techniques. In this study, we intend to provide basic data on the development of cosmetic products by measuring the antioxidants of mountain ginseng adventitious roots extracted from each ethanol concentration and the activity of tyrosinase and elastase enzyme inhibition. Methods and Results : After drying, the mountain ginseng adventitious roots, it was crushed to less than 0.5 ㎜. for testing. It was then extracted at 70℃ for 180 minutes, adding 10 times its weight to each ethanol concentration (0, 20, 40, 60 and 80%) and filtered. The extract is depressurised and used in experiments after freeze-drying. The total phenols and flavonoids content measurement showed the most content in 80% ethanol extract. However, Trolox equivalent antioxidant capacity, ferric reducing antioxidant power showed the best ability in 60% ethanol extract. In addition, the active measurements of tyrosinase and elastase enzyme inhibition performed to check skin whitening and wrinkles improvement showed the highest activity in the 80% ethanol extract. Conclusion : As a result, the optimum conditions for the use of natural antioxidants and functional foods were maximized when it is extracted with 60% ethanol. It is also expected to be valuable as a natural cosmetics material when extracted with 80% ethanol as a solvent when combined with the results of enzyme inhibition activity.

Background : Extraction is the first and the most important step in the recovery and purification of bio-active compounds from plant materials. Many important factors such as solvent, solvent composition, solvent to solid ratio, pH, and temperature significantly influence the extraction efficiency of bio-active compounds. Factorial design of a limited set of variables is advantageous when compared to the conventional method which varies a single parameter per trial. Methods and Results : The objective of this study was to screen independent factors, namely, ethanol concentration (60 – 100%), extraction temperature (40 – 80℃), time (6 – 18 hours), and liquid to solid ratio (10 – 50 ㎖/g) on the recovery of the extract yield, antioxidant capacity, phenolic and flavonoid contents from Dendropanax morbifera leaf using factorial design. Total flavonoid content of extract was determined by colorimetric method with aluminum chloride, while antioxidant activity was screened using the DPPH radical scavenging activity, TEAC and FRAP assays. Full factorial design was employed to determine the significant contribution of the above factors towards antioxidant capacity (TEAC, DPPH and FRAP), and flavonoid contents. Among, all the factors examined, ethanol concentration and extraction temperature are very significant (p < 0.0001), in obtaining higher antioxidant activity, total flavonoid contents. Conclusion : Two level full factorial design screening was successfully employed to determine the significant factors, which are ethanol concentration, temperature, time and liquid to solid ratio in contributing to high antioxidant capacity (TEAC, DPPH and FRAP), and flavonoid content determination from Dendropanax morbifera leaf. From the results obtained, ethanol concentration and temperature was very significant (p < 0.0001), in obtaining higher antioxidant activity and flavonoid contents. Further work on optimization using these significant factors are in progress.

Background: The biological activities of Tradescantia pallida grown in Korea have not been well determined, thus the aim of this study was to investigate the possibility of using it as a medicinal plant. Methods and Results: To investigate the antioxidant activity, α-glucosidase inhibitory effect and antimicrobial activity of T. pallida, we performed the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and reducing power assay. This assay for T. pallida leaf extract showed the highest antioxidant activity for the ethyl acetate fraction (RC50= 14.55 ± 0.16㎍/㎖ and Abs = 0.613 at 300㎍). Further, the ethyl acetate fraction exhibited higher α-glucosidase inhibitory effect with an IC50 value of 14.1 ± 0.1㎍/㎖ and showed antimicrobial activity against gram positive bacteria (minimum inhibitory concentration = 1,000㎍/㎖). Conclusions: The ethyl acetate fraction of the crude methanol extract of T. pallida showed remarkable antioxidant activity, α-glucosidase inhibitory effects and antimicrobial activity. These activities might be related to the flavonoid content in the T. pallida leaf extract.