The present study was conducted to analyze agronomic characteristics of 8 ecotypes of Arundinella hirta (A. hirta) and the correlation among them. Changes in phenotypic characteristics of 8 ecotypes were measured at equal intervals of time from May to September. Among ecotypes, Jangsoo-1 has the highest plant height (172.33 cm), number of leaves (9.00) and leaf length (55 cm) while the ecotype Youngduk has the highest leaf width (1.57 cm), fresh mass (26.63 g), dry mass (7.06 g), number of spikelets per spike (53.33), amount of seeds per spike (0.74 g) and amount of seeds per 10 spikes (7.23 g). The ecotype Jinju-1 has the shortest plant height (119 cm) and leaf number (6.33), while Okgye-2 has shortest leaf length (30.67 cm), leaf width (0.93 cm), fresh mass (12.60 g), dry mass (3.30 g), spike length (30.33 cm), spikelet per spike (39.67), amount of seeds per spike (0.61 g) and amount of seeds per 10 spikes (6.00 g). Correlation coefficients were estimated among the studied agronomic characteristics which showed positive and significant association with each other. In the present study, the agronomic data collected would be useful to understand the potential of A. hirta as a forage resource and helpful in selecting the high-yielding genetic resource for future forage improvement.

The increase in temperature due to climate warming is predicted to affect crop yields in the future. Until now, various types of OTC (open top chamber) that simulate the future climate condition have been developed and used to study the effect of temperature increase due to global warming on maize growth. However, in most OTCs, high equipment and maintenance costs were required to artificially increase the temperature. This study was carried to develop a cost-effective and simple OTC suitable for climate warming experiments for forage maize. Three octagonal OTCs with a height of 3.5 m × a diameter of 4.08 m and a partially covered top were constructed. The lower part of OTC covered film was opened at a height of 26 cm (OTC-26), 12 cm (OTC-12) from the ground surface, or not opened (0 cm, OTC-0). Mean air temperatures during the daytime on a sunny day in OTC-0, OTC-12 and OTC-26 increased to 3.23℃, 1.33℃, and 0.89℃, respectively, compared to the ambient control plot. For a pilot test, forage maize, ‘Gwangpyeongok’ was grown at OTCs and ambient control plots. As a result, in the late maize vegetative growth phase (July 30), the plant height was increased more than 45% higher than the ambient control plot in all OTC plots, and the stem diameter also increased in all OTC plots. These results indicate that it is possible to set the temperature inside the OTC by adjusting the opening height of the lower end of the OTC, and it can be applied to study the response of forage maize to elevated temperature. An OTC, with its advantages of energy free, low maintenance cost, and simple temperature setting, will be helpful in studying maize growth responsiveness to climate warming in the future.

이 연구는 해파리 비료 시비처리 수준에 따른 상수리나무 유묘의 광합성 반응과 엽록소 함량 변화를 알아보기 위해 수행되었으며, 시비처리는 5가지로 조절하였다(무시비처리구, 1.6 g/kg, 2.7 g/kg, 5.5 g/kg과 11.1 g/kg). 해파리 비료 시비처리시 토양수분함량과 잎의 수분포텐셜은 비교적 높게 나타났으며, 해파리 비료의 콜로이드 특성이 토양의 수분보유력을 높이는 것을 알 수 있다. 1.6 g/kg 처리구에서 최대 광합성속도, 기공증산속도, 기공전도도, Fv/Fm 및 토양-엽 수리전도도 등 전반적인 광합성 활성이 증가 하였으며(p<0.05), 엽록소와 카로테노이드 함량 및 Fv/Fo 역시 높게 나타났다(p<0.05). 이와 유사하게 2.7 g/kg 처리구 역시 광합성 활성과 엽록소 함량이 높은 것으로(p<0.05) 나타났으나 무처리구는 이와 반대되는 경향을 보였다. 특히 11.1 g/kg 처리구 경우 토양수분함량은 가장 높았으나 잎의 수분포텐셜은 상대적으로 낮게 나타났는데, 이는 과도한 시비에 따른 생리적인 장애로 생각된다. 결론적으로 1.6 g/kg과 2.7 g/kg 해파리 비료 시비처리시 상수리나무 유묘의 토양수분 보유력과 광합성 활성이 증가됨을 알 수 있다.

본 연구는 조선기자재 수출입 기업체를 대상으로 설문조사를 실시하여 수출입 물류에 관한 일반사항과 물류실태, 화물정보 제공, 화물 식별 및 분류, 화물 오배송 및 손상 등의 화물관리 상의 애로요인과 개선방안을 조사, 분석하여 조선기자재 수출입 물류에 있어서 화물 관리의 문제점과 해결방안을 제시하였다. 조선기자재 수출입 물류가 원할히 이루어지기 위해서는 수출입 업체와 물류관련 업체 간의 화물상 태와 위치에 관한 정보가 실시간으로 제공되어야 할 것이다. 그리고 수출입 화물의 지연과 소실 및 오배송을 줄이고 물류효율성을 제고하기 위해서는 표준 화물 분류시스템과 인식시스템의 제정이 시급히 이루어져야 할 것이다. 또한 조선기자재 전문 포워더의 육성과 전문성 강화 가 필요하며, 이를 위해 포워더 담당자들에 대한 역량배양 교육을 수출입 업체와 물류업체가 공동으로 참여하여 조선기자재 제품 특성과 물 류 특성을 모두 반영하여 수행하여야 할 것이다.

We isolated low temperature inducible genes using suppression subtractive hybridization (SSH) method and were able to obtain to clone MLT7 gene encoding peroxiredoxin and aminotransferase. The full-length cDNA of MLT7 is 1,049 bp with an open reading frame (ORF) consisting of 261 amino acid (aa). Genomic southern blot confirmed that mungbean genome has two copies of MLT7 gene. Northern blot analysis was also carried out for the gene expression during ABA, NaCl, drought, wounding and H2O2 stresses. The expression of MLT7 gene significantly decreased by ABA, NaCl and drought stress, but wounding and H2O2 stress significantly induced MLT7 gene expression. Especially, H2O2 strongly induced the MLT7 gene expression. The expression of MLT7 gene during low temperature stress started to increase in 3 h after treatment, and than slightly decreased and again increased at 24 h. Using GFP fusion vector, GFP-MLT7 was targeted both to mitochondria and chloroplast. However, it was mostly targeted to mitochondria and partially targeted to chloroplast. For the functional analysis of MLT7, MLT7 recombinant protein was heterologously expressed in E. coli. The MLT7 recombinant cells showed enhanced antioxidant activity compared to that of vector control cells.

We isolated low temperature inducible genes using suppression subtractive hybridization (SSH) method and were able to obtain to cloneMLT107 gene encoding peroxiredoxin and aminotransferase. The full-length cDNA of MLT107 is 1,049 bp with an open reading frame (ORF) consisting of 261 amino acid (aa). Genomic southern blot confirmed that mungbean genome has two copies of MLT107 gene. Northern blot analysis was also carried out for the gene expression during ABA, NaCl, drought, wounding and H2O2 stresses. The expression of MLT107 gene significantly decreased by ABA, NaCl and drought stress, but wounding and H2O2 stress significantly induced MLT107 gene expression. Especially, H2O2 strongly induced the MLT107 gene expression. The expression of MLT107gene during low temperature stress started to increase in 3 h after treatment, and than slightly decreased and again increased at 24 h. Using GFP fusion vector, smGFP-MLT107 was targeted both to mitochondria and chloroplast. However, it was mostly targeted to mitochondria and partially targeted to chloroplast. For the functional analysis of MLT107, MLT107 recombinant protein was heterologously expressed in E.coli. The MLT107 recombinant cells showed enhanced antioxidant activity compared to that of vector control cells.