본 연구는 향버섯이 발생하는 임지를 대상으로 식생구조와 토양 특성을 구명하여 임지 및 환경의 필수적인 기초자료 를 제공하고자 수행하였다. 연구대상지는 신갈나무가 우점하고 있는 신갈나무군락과 굴참나무, 신갈나무, 소나무 등이 혼재하고 있는 혼효림 형태인 굴참나무군락으로 구분되었다. 또한 전자의 군락에는 실새풀, 후자의 군락에는 애기나리 와 대사초가 지표종으로써 유의한 수준으로 나타났다. 두 군락 모두 유효인산과 pH, 모래, 점토가 강한 상관성을 나타내었으며, 토양의 높은 유기물 함량과 약산성 pH는 향버섯 발생에 관련된 것으로 판단된다. 하위 층위에 나타나는 종들은 상위 층위에 출현한 종들이 실현 지위를 확보하고 있기 때문에 지속적으로 우점하는 것으로 나타났다. 해당 입지는 북-동의 방위로 비교적 습윤한 입지에 분포하였고, 낙엽층 깊이가 얇고 암석노출도가 낮아 토양이 발달한 것으로 보였다. 선정된 향버섯 발생지들은 교란 또는 외부 간섭으로부터 적응성이 높은 종을 중심으로 식생이 발달하고 있었다. 이와 같은 사실을 통해 향버섯이 발생할 수 있는 임분을 간접적으로 파악할 수 있으며, 인공재배 시 향버섯 발생 촉진을 위한 기초자료로 활용될 수 있을 것으로 기대된다.

In this study, machine learning models are proposed to predict the Vickers hardness of AlSi10Mg alloys fabricated by laser powder bed fusion (LPBF). A total of 113 utilizable datasets were collected from the literature. The hyperparameters of the machine-learning models were adjusted to select an accurate predictive model. The random forest regression (RFR) model showed the best performance compared to support vector regression, artificial neural networks, and k-nearest neighbors. The variable importance and prediction mechanisms of the RFR were discussed by Shapley additive explanation (SHAP). Aging time had the greatest influence on the Vickers hardness, followed by solution time, solution temperature, layer thickness, scan speed, power, aging temperature, average particle size, and hatching distance. Detailed prediction mechanisms for RFR are analyzed using SHAP dependence plots.

본 연구는 온대 낙엽활엽수림의 하층식생 변화를 구명하여 산림생태계의 체계적 보전과 효율적 관리를 위한 기초자료를 제공하기 위하여 수행하였다. 2003년 경기도 포천 광릉 숲에 1ha 크기의 영구조사구를 설치하였으며, 영구조사구는 10×10m 크기의 부조사구 100개로 구성되었다. 영구조사구의 임분동태 및 하층식생은 2003년부터 2018년까지 5년 간격으로 조사되었다. 조사구에 출현하는 관속식물은 56과 128속 176종 18변종 4품종 1아종으로 총 199분류군 이었다. 관목층과 초본층의 종수는 시간이 경과함에 따라 모두 감소하는 경향을 보였다. MRPP-test 분석 결과 관목층의 종조성은 2008년-2013년을 제외한 모든 연도에서 유의한 차이가 있는 것으로 나타났으며, 초본층의 경우 모든 연도에서 유의한 차이가 있는 것으로 나타났다. 평균 중요치에서 관목층은 참회나무(18.23%), 당단풍나무(16.48%), 작살나무(13.85%)가 우점하는 것으로 분석되었으며, 초본층에서는 단풍취(23.41%), 애기나리(9.45%), 주름조개풀(5.62%)이 우점하는 것으 로 나타났다. 관목층은 상층 임분의 흉고단면적과 임분밀도가 높을수록 청미래덩굴, 청괴불나무, 고광나무의 풍부도가 높았으며, 흉고단면적과 임분밀도가 낮을수록 당단풍나무, 물참대, 산뽕나무, 산딸나무의 풍부도가 높았다. 시간이 경과 할수록 흉고단면적과 임분밀도는 초본층에 미치는 영향이 적은 반면, 관목층의 참회나무와 당단풍나무의 피도는 초본층 종 구성에 미치는 영향이 큰 것으로 분석되었다. 결론적으로 광릉 장기생태조사지의 하층은 종수가 지속적으로 감소하고 있으며, 이 과정은 상층 임분의 종다양성과 흉고단면적, 임분밀도가 하층식생 종조성에 영향을 주는 것으로 판단되었다.

본 연구는 일본잎갈나무림의 벌채유형을 대조구, 보잔목작업구, 모수작업구, 군상개벌작업구, 대상개벌작업구로 구분하여 벌채 처리 전·후의 하층식생 변화를 분석하기 위해 수행되었다. 경북 봉화 지역에서 2014년부터 2016년까지 벌채유형별로 각 9개씩 총 45개 조사구를 대상으로 방형구법을 이용하여 하층식생을 조사하였다. 군상개벌작업구의 총 피도가 지속적으로 높게 나타났으며, 벌채를 실시한 처리구에서는 광선호성 종들이 우점하고 있는 것으로 나타났다. 보잔목작업구와 군상개벌작업구, 대상개벌작업구에서는 노출된 임분에 잘 적응하는 소형지상식물 (Microphanerophyte)의 비율이 가장 높게 나타났으며, 이는 지속적으로 증가할 것으로 판단되었다. 종조성 변화에서는 다른 처리구들과 다르게 보잔목작업구가 가장 큰 변화를 보인 것으로 나타났다. 처리구 간 분석 결과, 대조구와 군상개벌작업구의 두 처리구 간 종조성 차이는 이질적인 것으로 나타났다. 결론적으로, 일본잎갈나무 조림지에서 수행한 4가지 벌채 처리는 모두 대조구에 비해 식물다양성이 증가하는 경향을 보였다. 그러나, 하층식생의 발달 뿐 만 아니라 일본잎갈나무의 천연갱신을 유도하기 위해서는 5년∼7년의 결실주기를 가진 일본잎갈나무 특성상, 지속적인 모니터링 연구가 필요할 것으로 판단된다.

최근 관광, 레저 수요의 증대로 해안 시설물(방파제)에서 낚시를 통해 여가를 즐기는 낚시객의 증대와 아울러 매년 해안 시설물인 소파블록(Tetrapod, TTP)에서의 낙상사고가 사회적 문제가 되고 있다. 본 연구에서는 낙상사고가 주로 발생하는 소파블록을 안전망으로 덮어 낚시객의 낙상을 저감하는 안전사고 방지 시설물 개발의 기초 연구로서 적절한 그물코 크기를 제시하고자 한다. 이는 안전망 시설물의 규모 및 경제성을 고려할 때 반드시 선행되어야 할 부분이다. 본 연구에서 적용한 안전망의 그물코 크기 결정 방법은 낚시객들의 성별, 연령별 현황과 한국인의 인체치수조사 결과를 바탕으로 최적의 그물코 크기를 산정하고자 하였다. 그 최종 결과로서 안전망의 그물 코 크기는 최소 낚시객의 넙다리 둘레에서부터 최대 가슴둘레까지를 고려하여 18.6~27.0 cm의 범위로 산정되었다.

목적 : 청광 차단렌즈의 효과를 정량적으로 분석하는 방법을 새롭게 제시하고, 이를 시판하고 있는 기능성 렌즈에 적용하여 비교 분석함으로써 그 활용 가능성을 확인하고자 하였다. 방법 : 렌즈의 광투과율을 측정하고, 청광 차단 효율 함수를 적용하여 효율에 대한 정성적인 분석을 하였다. 또한 청광 차단 효율 결과를 적분하여 수치화하는 정량적인 분석 방법을 새롭게 도입하여, 시판중인 청색광차단 기능성 렌즈에 적용하여 비교분석하였다. 결과 : 정성적인 분석 방법을 일반렌즈, 코팅방법으로 제작된 2종의 렌즈와 착색방법으로 제작된 1종의 렌즈에 적용한 결과, 모든 렌즈는 단파장 영역의 빛을 차단하는 효과가 우수한 것으로 분석되었다. 하지만 정량적인 분석 방법에 의한 결과에서는 각 렌즈별 다소 차이가 있음을 확인하였다. 결론 : 다양해지는 디스플레이 장치 및 스마트폰의 광 특성이 날로 진화되고 변화되고 있으므로, 다양한 방법으로 광원과 광학제품에 대한 광특성 분석이 요구된다. 이에 따라, 본 연구에서 새롭게 도입한 정량적인 분석 방법이 효과적인 수단일 될 수 있는 가능성을 확인 하였다.

Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.

지적장애아동은 낮은 지적능력으로 인해 주의집중력의 지속시간이 짧으며 정상적인 언어습득과 발달이 어렵다. 그러므로 사회성이 떨어지고 사람과의 유대감을 형성하는 것이 어려울 수 있다. 지적장애아동의 특성을 효과적으로 개선하는 방법에는 동물을 통한 매개 활동이 있다. 동물은 아동과의 상호작용을 통하여 언어능력을 향상 시키고, 인지발달을 촉진시킨다. 그리고 의사소통이나 상호작용이 미숙한 아동에게 동물매개활동은 의사소통 및 자기표현 기술을 습득하는 데 유용하게 작용할 수 있다. 본 연구는 동물매개활동 프로그 램이 지적장애아동의 주의집중력과 언어능력에 미치는 효과를 알아보기 위해 J도 I시 특수 장애 학교에 다니고 있는 만 14세에서 16세인 지적장애아동 3명을 대상자로 선정하였다. 연구는 주 1회, 8주간, 총 16회기 동안 진행하였다. 사전ㆍ사후 검사 도구로는 Harris & Harris(1984)의 집중력 격자판 검사와 수용·표현 어휘력 검사(Receptive and Expressive Vocabulary Test: REVT)를 이용하여 연구 대상자들의 주의집중력과 수용·표현 어휘력의 비교분석을 하였다. 이 연구를 통해 동물매개활동 프로그램이 지적장애아동의 주의집중력과 수용·표현 어휘력을 유의미한 수준으로 향상시켜 주었음을 관찰할 수 있었다.

Interferon-tau (IFNT) is known as a major conceptus protein that signals the process of maternal recognition of pregnancy in ruminants. Also, multiple interferon genes exist in cattle, However, molecular mechanisms of these bovine IFNT (bIFNT) genes whose expressions are limited have not been characterized. We and others have observed that expression levels of bovine subtype IFNT genes in the tissues of ruminants; thus, bIFNT1 and other new type I (bIFNTc1/c2/c3) gene co-exist during the early stages of conceptus development and non-trophoblast cells. Its genes transcription could be regulated through CDX2 and ETS2 and JUN and/or cAMP-response element binding protein (CREB)-binding protein (CREBBP) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. Bovine ear-derived fibroblast cells, were co-transfected with luciferase reporter constructs carrying upstream (positions -1000 to +51) regions of bIFNT1 and other new type I gene and various transcription factor expression plasmids. Compared to each - 1kb-bIFNT1/ c1/c2/c3-Luc increased when this constructs were co-transfected with CDX2, ETS2, JUN and/or CREBBP. Also, Its genes was had very effect on activity by CDX2, either alone or with the other transcription factors, markedly increased luciferase activity. However, the degree of transcriptional activation of the bIFNTc1 gene was not similar to that bIFNT1/c2/c3 gene by expression plasmid. Furthermore, Sequence analyses also revealed that the expression levels of bIFNT1/c2/c3 gene mRNAs expression were highest on day 17, 20 and 22 trophoblast and, Madin-Darby bovine kidney (MDBK), Bovine ear-derived fibroblast (EF), and endometrium (Endo) non-trophoblast cells. But, bIFNTc1 mRNA had not same expression level, bIFNTc1 lowest levels than those of IFNT1/c2/c3 gene in both trophoblast and non-trophoblast cells. These results demonstrate that bovine subtype bIFNT genes display differential, in the trophoblast and non-trophoblast cells.

Variance of conceptus interferon tau (IFNT), produced by the embryonic trophectoderm, is known as a major conceptus protein that signals the process of maternal recognition of pregnancy in ruminants, essential for the maintenance of early pregnancy. Similar to other IFN genes such as IFNA and IFNB, multiple IFNT genes are present. However, some kinds of IFNT genes actively transcribed and regulated in bovine conceptuses have not been well characterized. In this study, during the course of bovine IFNT gene transcription through the use of next generation sequencer SOLiD3, revealed that among 38 IFN genes registered, only two transcripts, IFNT1 and IFNTc1, were found in conceptuses during early pregnancy. Also, to identify a transcription factor(s) involved in the regulation of IFNT genes, mRNAs for various known transcription factors were investigated by real-time PCR in conceptus tissues, respectively. Furthermore, compared to the IFNT genes, IFNT1 and IFNTc1 had same active levels, which were previously shown to correlate with the appearance of effective antiviral activity. However, the expression levels of these Luc activities differed. Bovine ear fibroblast (EF) cells were cotransfected with luciferase reporter constructs carrying upstream (–631 to -51) promoter regions of IFNT1 or IFNTc1 and various transcription factor expression plasmids, CDX2, AP1(JUN), ETS2 and/or cAMP-response element binding protein (CREB)-binding protein (CREBBP). CDX2, either alone with the other 2 transcription factors, was found to increase luciferase activity approximately 14- and 11-folds, respectively. The degree of transcriptional activation of the IFNTc1 gene was not similar to that IFNT1 gene by AP1, ETS2 or/and CREBBP, expression plasmid. These results suggest that two isoforms of bovine conceptus IFNT genes are regulated differently in conceptuses during early pregnancy.

The present study was to assess the in vivo embryo production efficiency using the semen separated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7 of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 days after the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificial insemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4 million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observed in the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbers of total embryos are each 12.58 ± 8.31 and 13.25 ± 7.86. The mean numbers of transferable embryos, sexed semen were significantly lower than KPN semen (3.75 ± 1.98 vs. 8.23 ± 6.07, P<0.05). The rates of unfertilized embryos from superovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate of degenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion, these results indicate that superovulation using sexed semen was useful, but efficient embryo production was important to reducing the damage caused by the Flowcytometer-based sperm sorting procedure.

Although some factors, including season, age, type of estrus (natural estrus vs. induced estrus) and semen type (conventional vs. sexed), affect the conception rate following artificial insemination (AI) in dairy cattle, there is little information about the influence of ovarian characteristics, such as preovulatory follicle (PF) location at estrus, on fertility in dairy cattle. In most breeds of cattle the right ovary appears to function more actively than the left and about sixty percent of pregnancies in dairy cattle occur in the right horn of uterus (Reece and Tuner, 1938). Our study aimed to compare conception rates in dairy cattle between PFs that developed in the left ovary and those that developed in the right ovary at estrus. In this study, we examined the locational effect (left or right ovary) of the preovulatory follicle (PF) on fertility in dairy cattle. In total, 955 artificial inseminations (AI) were analyzed. At AI, PF locations were examined using rectal palpation, and dairy cattle were divided into two groups on their PF locations: (i) the PF located in the left ovary (L-PF); and (ii) the PF located in the right ovary (R-PF). Pregnancy was diagnosed by rectal palpation or ultrasonographic examination 60 days after AI. The conception rate was 38.1% in all dairy cattle. Conception rate was higher in the R-PF (40.8%) than in the L-PF (33.2%). In summary, PF development in the right ovary was associated with increased conception rates in dairy cattle.

본 연구는 생물다양성이 매우 중요하게 여겨지고 있는 백두대간 일대인 단목령-구룡령 구간의 산림식생을 보다 더 생태적이고 체계적인 보전 및 관리 계획 수립을 위한 기초자료를 제공하고자 수행하였다. 총 조사구 142개소에 대해 Z.-M. 학파의 식물사회학적인 방법을 사용하였고 식생조사 및 입지 환경 특성을 조사하였다. 산림식생의 유형은 1개 군락군, 2개 군락, 4개 군, 4개 소군의 단위체계를 가진 것으로 분석되었는데 상재도급 Ⅴ형에 해당하는 신갈나무와 당단풍나무는 대체적으로 향존중으로 나타났으며, 조사 지역의 산림생태계 관리 계획에 있어 중점관리대상종이 될 것으로 판단되었다. 그리고 종다양성에 대한 결과를 살펴보면 VT3형에서 우점종들이 균일하게 분포되어 있었고 VT4 형의 종들은 다양하게 분포하고 있었을 뿐만 아니라 종들이 제일 풍부하게 나타났다. 또한, 단목령-구룡령 구간에서는 희귀식물이 다소 분포하였는데 멸종위기종인 벌깨풀과 위기종인 구실바위취, 취약종인 백작약, 두메대극, 금강초롱꽃, 어리병풍, 약관심종인 등칡, 도깨비부채, 참배암차즈기, 미치광이풀, 병풍쌈 등 총 24종의 희귀식물이 나타났다.

To increase the productivity of in vitro development, the antioxidants have been used for culture system of bovine oocytes and embryos. However, comparative studies on these molecules are rare and direct beneficial effects on blastocyst production cannot be discriminated for best results. The study was conducted to determine the influence of N-acetyl-L-cysteine (NAC), N-acetyl-L-cysteine amide (NACA), glutathione (GSH) and cysteamime (CYS) on maturation competence of COCs from GV to MII stage and productivity of blastocyst formation during in vitro fertilization and culture. There was no difference among maturation rates of oocytes to metaphase II with polar body with antioxidants for any of the treatment groups (p>0.05). However, the significant improvement on the rate of blastocysts (32.3±5.0%) was found in 0.1 mM CYS treatment than 0.3 mM NAC, 0.2 mM NACA or 0.5mM GSH (p<0.05). The addition of NAC (18.8±3.7%) or NACA (21.2±3.9%) did not improve development competence to morula and blastocysts than control (24.4±4.1%) and GSH (26.5±5.0%) (p>0.05). Our study showed that medium supplementation with CYS during IVM and IVC improved the rate of bovine embryo development but not with NAC, NACA and GSH addition.

The elevated temperature and high humidity has been known as main reason for heat stress on animals and cause detrimental effects on productivity of organisms and physiological conditions of normal bioactivities. The aims of this study were to evaluate the relationship between time of heat shock simulation during in vitro maturation and developmental competence of subsequent embryo after in vitro fertilization. Heat shocked cumulus-oocyte complexes (COCs) of Korean native cattle were subjected to normal conditions for 22, 21, 18 and 12 h respectively and transferred to heat stress inducing condition at 40.5 °C in other incubator for 0 (control), 1 and 4 h. After maturation for 22 h, the oocytes were fertilized and cultured in mSOF media for 8 d and examined the developmental capacity of embryos. There were no differences in maturation and cleavage rates between 0, 1 and 4 h heat socked oocytes, but blastocysts formation were lower in the 4 h heat stressed oocytes. The apoptotic cells of developed blastocysts were also increased in at day 8 with 4 h heat shocked oocytes. These results indicate that heat shock on oocytes during maturation could cause negative effects on the developmental competence of embryos.

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudalrelatedhomeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.

Interferon tau (IFNT), produced by the mononuclear trophectoderm, signals the process of maternal recognition of pregnancy in ruminants, but its expression in vivo is not well characterized. Objectives of this study were to determine IFNT gene isoforms expressed in the bovine uterus, and to identify differences in promoter sequences of IFNT genes that differ in their expression. Through the RNA-seq analysis of bovine conceptuses on days 17, 20 and 22 (day 0 = day of estrus), the expression of only two IFNT transcripts, IFNT1 and IFNTc1, were found, which were indeed classified into the IFNT gene clade. IFNT mRNAs were highest on day 17, and then decreased on days 20, and 22, which were also supported by the results of quantitative RT-PCR. Bovine ear-derived fibroblast (EF) cells were then cotransfected with luciferase reporter constructs carrying 5‘-upstream (positions -1000 to +51) regions of IFNT1 or IFNTc1 and various transcription factor expression plasmids. CDX2, either alone or with other Ap-1, ETS2 and/or CREBBP transcription factors, was found to increase luciferase activity approximately 10 and 18 fold more than twice of those cotransfected with bIFNT1, c1-Luc construct. Furthermore, The degree of transcriptional activation by a combination of the AP1, ETS2, CREBBP and/or CDX2 expression vectors was similar to that of CDX2 along plasmid. However, expression patterns of these Luc activity differented. Whereas bIFNTc1-Luc showed lowest antivity had than bIFNT1-Luc reports. Although, lowest antivity had of the bIFNTc1 –Luc report, cotransfected with the bIFNTc1-Luc construct and AP1(JUN) or/and ETS2 expression plasmid, Luc activity was enhanced approximately 2 and 4-fold more than the bIFNT1-Luc. Furthermore, along CDX2 expression factor had high effect on activity of bIFNT1-Luc reporter than the c1 gene in EF cells. These results suggest that two forms of IFNT genes are expressed in utero and their transcriptional regulations differ.

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), one of new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome or not and to examine the expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. The transcription of these genes could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1(JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on the days 17, 20 and 22 bovine conceptuses. bIFNT1 was highly expressed on the day 17 and transcripts were gradually and weakly detectable on the days 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on the day 20 and transcripts were weakly detectable on the days 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had higher effect on activity by alone ETS2, and AP1(JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not idented. These results demonstrate that these genes display differential, tissue-specific expression and developmental regulation during pregnancy.

Ethyl formate (EF), a methyl bromide alternative fumigant which is commercially used for quarantine and pre-shipment(QPS) purpose, has a potential to control various insect pests. Based on CTP (concentration × time products), we've firstly evaluated fumigation trials of EF on cucumber plants to eradicate horticultural insect pests in greenhouse. In the preliminary test, EF achieved 100% control of cotton aphid, western flower thrip and tobacco whitefly when CTP of EF was 4.4, 5.6 and 3.3 g h m-3, respectively. In 1300m3 scale cucumber greenhouse trial, EF was applied with 2g m-3 dosage for 15 hrs, and cotton aphid, western flower thrip and tobacco whitefly were completely controlled without phytotoxic symptom. EF fumigation could be suitable for greenhouse cultivation in terms of good efficacy on horticultural insect pests with labor-saving benefit than current insecticide spraying applications.