고령운전자의 인지 및 신체 기능 저하로 인한 교통사고 증가는 점점 심각한 사회적 문제가 되고 있으며, 이 로 인해 면허증을 자진하여 반납하는 제도가 운영되고 있으나 보다 객관적인 평가 방법이 필요하다. 본 연 구에서는 다양한 주행 상황에서 발생하는 정적 및 동적 시각 자극에 대하여 운전자의 시각적 행동을 평가 할 수 있는 운전 시뮬레이션 주행 환경과 시나리오를 구현하는 것이 목표이다. 이를 위해 고령운전자의 주 행 특성 분석에 활용된 운전 시뮬레이션 시나리오를 기존의 연구 문헌들로부터 수집하였고, 정적 및 동적으 로 구분된 표적 자극에 따른 텍스트 네트워크 분석을 통해 주행환경과 시나리오를 재분류하였다. 또한, 유 사한 유형의 시나리오들은 보다 발생 빈도가 높고 표적 자극이 다양한 주행환경으로 병합하였다. 연구 결과 로 신호교차로, 비신호 교차로, 왕복 2차로, 왕복 4차로 주행환경에서의 전체 12종의 시나리오로 구성된 운 전 시뮬레이션 콘텐츠가 구현되었다. 신호등, 정차된 차량, 표지판 등으로 구성된 정적 시각 자극과 주행 중 인 차량, 무단 횡단하는 보행자 등의 동적인 시각 자극이 제시되며, 이에 대한 시선 탐색(Visual Detection), 시지각(Visual Perception), 시각운동기능(Visuomotor Function)의 정량적 측정을 통해 운전자의 시각 행동 평가가 이루어진다. 본 연구에서 제안하는 시나리오 구성 방법은 운전 시뮬레이션 콘텐츠를 구현하기 위한 새로운 접근 방식으로써 시각 자극에 따른 운전 능력 검사 환경 구축에 관한 기초 자료로 활용될 수 있을 것으로 기대된다.
해체의 최종단계는 대상 부지 및 건물을 규제로부터 제외하는 것이다. MARSSIM은 부지를 개방하기 위한 최종현황조사를 수행함에 있어 요구되는 자료의 수집 등의 다양한 요건을 만족키기 위한 총괄적인 지침을 제 공한다. 연구로 해체 후 부지 및 건물의 최종현황조사를 위해 MARSSIM에서 제시하고 있는 방법을 적용하였 다. 연구로 부지의 특성을 반영한 개방기준을 도출하기 위해 RESRAD 및 RESRAD-Build 전산코드를 이용하여 부지 및 건물에 대해서 계산하였다. 부지 및 건물의 조사설계(Survey Design)를 위해서 잠재적 오염도 및 측정 결과를 활용하여 조사구역을 구분하였고, 개략조사 및 특성조사를 통해 수집된 다양한 결과에 기초하여 통계 학적 검사를 통해 조사구역 별로 요구되는 시료의 수를 산정하게 된다. 측정된 결과에 기초하여 연구로 최종 현황조사는 개방기준에 만족하는 것으로 평가되었다.
Suaeda japonica is a halophytic plant that lives in mudflat at intertidal zone of western and southern coastal areas of Korea. The seawater-living plants showed a purple color during their whole life. In contrast, freshwater-living plants displayed a green color in leaves. When seawater-living plants were transferred to potting soil, the purple color was gradually changed to green in the leaves. The extracted purple pigment compound exhibited typical characteristics of betacyanin that were represented by water solubility, pH- and temperature-dependent color changes, sensitivity to light, UV-Vis spectra, and gel electrophoretic migration pattern. The LC-MS analysis of the extracted pigment compound showed the presence of two major protonated molecular ions ([M+H]+) at m/z 651.1 and m/z 827.1. Antioxidant activity of the pigment compound was determined using stable free radical DPPH assay. It was found to have an antioxidant activity that is linearly increased in proportion to the reaction time for up to 30 min, and the activity was comparable to that of control BHA at 9.0 ㎎/㎖. The anticancer activity against several tumor cell lines was also examined following the MTT assay. The significant growth inhibitory effect was observed on two tumor cell lines, SW-156 (human kidney carcinoma) and HEC-1B (human endometrial adenocarcinoma). Probably, the pigment compound may function as an osmolyte to uphold halotolerant physiological processes in saline environment.
Wild rice might have previously unidentified genes important for disease resistance and stress tolerance in response to biotic and abiotic stresses. A set of subtractive library was constructed both from leaves of wild rice plants, Oryza grandiglumis (CCDD, 2n=48), treated with fungal elicitor and from wounded leaves. A partial fragment that was homologous to PR10 genes from other plant species was identified via suppression subtractive hybridization and cDNA macroarray. The obtained full-length cDNA sequence (OgPR10) contains an open reading frame of 480 bp nucleotide, encoding 160 amino acids with a predicted molecular mass of 16.944 kDa and an isoelectric point (pI) of 4.91. The multiple alignment analyses showed the higher sequence homology of OgPR10 with PR10 genes identified in rice plants at amino acid level. The OgPR10 mRNA was not expressed by treatment with wounding, jasmonic acid, and salicylic acid, but markedly expressed in leaves treated with protein phosphatase inhibitors cantharidin and endothall, and yeast extract. In addition, the expression of OgPR10 mRNA was induced within 72 h after treatment with probenazole, one of well-known chemical elicitors, and reached the highest level at 144 h. Heterologous expression of OgPR10 caused growth inhibition and seedling lethality in E. coli and Arabidopsis, respectively. Chemically induced OgPR10 expression with glucocorticoid-mediated transcriptional induction system further reconfirmed its lethality on Arabidopsis seedling. In addition, OgPR10-expressing rice plants, Oryzae sativar were resistant against the infection of rice blast fungus, Magnaporthe grisea. These results indicate that OgPR10 is involved in probenazole- and microbe associated molecular patterns-mediated disease resistance responses in plants and is a potential gene for developing disease resistance crop plants.
The effects of the transferred green rice leafhopper (GRH) resistant gene on yield and grain quality were analyzed by using 7 near-isogenic lines (NILs) with introgressed GRH resistance gene in rice cultivar "Ilpumbyeo". The genotype analysis using SSR markers indicated that all NILs were recovered more than 95% as compared with recurrent parent "Ilpumbyeo". All NILs showed no significant differences compared to recurrent parent for grain yield. No significant differences were observed between NILs and their recurrent parent for physicochemical traits (protein content, amylose content and alkali spreading value) and eating quality. But there were significant differences for appearance of brown and milled rice and pasting properties between some NILs and their recurrent parent. It means that these grain appearance and pasting traits of NILs were very similar or more improved compared to recurrent parent "Ilpumbyeo". Thus, it could be possible to develop GRH resistant variety with high ,quality in segregating populations.
The objective of this study was to determine the role of storing years with the variation of total phenol and individual phenolic compounds in soybean (Glycine max L.) seeds. The total phenol content varied from 0.36 to 0.42% over four years, with the highest value (0.42%) found at storage for two and three years. Among the nine soybean varieties examined, Daweonkong had the highest total average phenol content (0.58%). The total content of 11 phenolics varied from 730.0 to 1812.8 ~mu~textrmg~;~textrmg-1 over storage for four years, and the highest concentration (1812.8 ~mu~textrmg~;~textrmg-1 ) was found at storage for two years. Myeongjunamulkong (1465.4 ~mu~textrmg~;~textrmg-1 ) had the highest mean content among the nine soybean varieties. The total content of 11 phenolic compounds measured in this study occupied from 20.96 to 47.73% of the total phenol contents. The highest total phenol contents were in seeds with black coats (5279.4 ~mu~textrmg~;~textrmg-1 ), while the highest concentration of individual phenolic compounds were in seeds with green coats (1419.5 ~mu~textrmg~;~textrmg-1 ). Our study suggests that it may be feasible to improve soybean varieties with high functional substances such as phenolic compounds.