This study was carried out to investigate the effects of seeding dates on the growth characteristics, dry matter yield and mineral contents of rye cultivated in the paddy field. The field experiment was conceived as a randomized block design performed in triplicate with seeding dates of October 19 (T1), October (T2), November 2 (T3), November 9 (T4), and November 16 (T5). All treatments was harvested on May 22 of the following year. Plant length, stem diameter, dry matter yield, and total digestible nutrient (TDN) yield were higher in rye with early seeding dates (p<0.05), whereas TDN was higher with late seeding dates (p<0.05). There was no significant difference between crude protein and ether extract among the different seeding dates. Neutral detergent fiber (NDF) and acid detergent fiber (ADF) were higher with early seeding dates (p<0.05). K and Ca contents were significantly higher at T1 as compared to other treatments (T2, T3, T4 and T5). There was no significant difference in Mg and Na content among T1, T2, T3, T4 and T5 treatments. P content was higher in the order T1 > T2 > T3 > T4 > T5 (p<0.05). There was no significant difference in free sugar contents (glucose, fructose and sucrose) by treatments, whereas total free sugar content was the highest in T1 than in the other treatments (p<0.05). Collectively, the results obtained in this study indicate that it is favorable to seed (T1 and T2) soon after harvesting rice to increase dry matter, TDN yield and total free sugar content of rye in the midlands of Korea.

This study was carried out to investigate the growth characteristics, yield and chemical compositions of whole crop barley (Hordeum vulgare L.) according to mixing ratio of chemical fertilizer (CF) and liquid swine manure (LSM) in the paddy field cultivation. The experimental design was arranged in a randomized block design with five treatments and three replications. The manure fertilizer ratio of five treatments were CF 100% (T1), CF 70% + LSM 30% (T2), CF 50% + LSM 50% (T3), CF 30% + LSM 70% (T4), and LSM 100% (T5) of whole crop barley. At this time, the application of liquid swine manure was based solely on the nitrogen. Plant length was higher at T1 as compared to other treatments (T2, T3, T4 and T5). Fresh yield, dry matter yield and total digestive nutrients (TDN) yield were the highest in T1, whereas the lowest in T5 treatment (p<0.05). Chemical compositions (crude protein, crude fat, neutral detergent fiber, acid detergent fiber and TDN) did not show significant difference among treatments. Ca and Na contents were significantly lower at T1 as compared to other treatments (T2, T3, T4 and T5). However, Mg and P contents were significantly higher at T1 as compared to other treatments(p<0.05). There was no significant difference in total free sugar content among T2, T3, T4 and T5 treatments, but the chemical fertilizer (T1) was significantly lower than the other treatments (p<0.01). Considering the above results, liquid swine manure application showed lower dry matter yield and TDN yield than chemical fertilizer, but higher free sugar content. Therefore, in order to increase the productivity of whole crop barley, it is considered desirable to mix liquid fertilizer with chemical fertilizer, taking into account the decomposition rate and insufficient components (P, K) of the liquid swine manure.

본 실험은 논 토양에서 호밀 재배시 화학비료와 발효 돈분 액 비 혼용 시용이 생육특성 및 영양성분에 미치는 영향을 조사하고 자 실시하였다. 실험설계는 화학비료 100% 처리구(C), 화학비료 70% +돈분액비 30% 처리구(T1), 화학비료 50% +돈분액비 50%처리구(T2), 화학비료 30% +돈분액비 70% 처리구(T3) 그리고 돈분액비 100% 처리구(T4)로 한, 5처리 3반복 난괴법으로 배치 하였다. 이때 돈분 액비 시용은 질소량만을 기준으로 하였다. 초 장은 처리구들 간에 유의적인 차이를 보이지 않았다. 이삭길이, 엽장 및 엽폭은 각각 C, T3 및 T2구에서 각각 높게 나타났다 (p<0.05). 경의 굵기는 T4 > T3 > T2 > T1 > C구 순으로 돈분 액비 시용량이 높을수록 높게 나타났다(p<0.05). 건물수량 및 TDN 수량은 T4구가 가장 높게 나타난 반면 C구가 가장 낮게 나 타났다(p<0.05). 조단백질, NDF, ADF 및 조섬유 함량에서는 처 리구들 간에 유의적인 차이는 나타나지 않았다. 그러나 조지방 및 조회분 함량에 있어서는 각각 T2 및 T3구에서 높게 나타났다 (p<0.05). 총무기물 함량은 돈분액비 시용 비율이 증가할수록 유 의적으로 감소하였다(p<0.05). 그러나 총 유리당 함량은 돈분액 비 시용 비율이 높았던 T3 및 T4구가 높게 나타난 반면 화학비료 처리구(C) 및 돈분액비 시용 비율이 낮은 구(T1)에서는 낮게 나 타났다(p<0.05). 이상의 결과를 종합해 볼 때, 수량성(건물수량, TDN수량)과 사일리지 품질에 영향을 미치는 유리당을 고려한다 면 화학비료와 돈분액비 혼용하거나 돈분액비를 시용하는 것도 효과적이라고 사료된다.

This study was carried out to investigate the effects of planting densities on the growth characteristics, dry matter yield, and feed value of “Geukdong 6” (a new variety of corn for feed). The experimental design was arranged in a randomized block design with three replications. Treatments consisted of six planting densities, 60 cm × 25 cm (T1), 60 cm × 30 cm (T2), 70 cm × 25 cm (T3), 70 cm × 30 cm (T4), 80 cm × 25 cm (T5) and 80 cm × 30 cm (T6). All treatments were sown on May 14, 2021, and the harvest was on October 3 (late flowering). Plant length and the number of tillers were the highest in T5 (p<0.05), but the number of leaves and stem diameter were the highest in T6 than in the other treatments (p<0.05). Leaf length, leaf width, and dead leaf were not significantly different among the treatments. Organic matter was highest in T6, and crude protein was highest in T5 (p<0.05). The ether extract was not significantly different among the treatments. Crude fiber, NDF, and ADF were highest in T2 with relatively higher planting density (p<0.05). Calcium and phosphorus were not significantly different among the treatments. TDN content was the highest in T3 (p<0.05). Sugar degree (Brix), fructose, glucose, dextran, isomerose, and inverted sugar were not significantly different among the treatment. Fresh yield, dry matter yield and TDN yield were higher in order of T6 > T5 > T4 > T3 > T2 > T1 (p<0.05). Relatively feed value was higher in order of T3 > T6 > T5 > T1 > T4 > T2 (p<0.05). Based on the above results, planting density could be recommended from 80 cm × 30 cm for efficient production of “Geukdong 6”.

본 실험은 Holstein 비육후기에 홍삼박, 광물질 및 설탕을 첨 가 급여하여 건물채식량, 일당증체량, 육질특성 및 경제성에 미치는 영향을 조사하고자 수행하였다. 시험구는 4처리로서 홍삼박, 광물질 및 흑설탕을 급여하지 않는 처리구를 대조구(C), 홍삼박 200g 첨가 급여 구는 T1, 홍삼박 100g과 광물질 50g 첨가 급여구는 T2 그리고 광물질 50g과 흑설탕 20g 첨가 급여한 구를 T3 로 나누어 실시하였다. 그리고 본 실험에서 얻어진 결과를 요약해 보면 다음과 같다. 총 사료섭취량 및 1일 채식량은 T2 및 T3구가 C구에 비하여 유의적으로 높게 나타났다(p<0.05). 또한 채식량 이 많았던 T2 및 T3구에서 총 증체량 및 일당 증체량이 높게 나 타났지만 유의적인 차이는 없었다. 사료 요구율은 C구가 다른 구 (T1, T2, T3)에 비하여 유의적으로 높게 나타났지만(p<0.05), T1, T2 및 T3 구간에는 유의적인 차이가 없었다. 등지방두께, 등심단 면적 및 육량등급은 처리구 상호간 유의적인 차이가 없었다. 근내 지방은 T3(4.5) > T1(4.0) > T2(2.7) > C(2.5)구순으로 높게 나타났다(p<0.05). 그러나 육색, 지방색, 조직감 및 성숙도는 상호 처리 간 유의적인 차이가 없었다. 육질등급은 상호 처리 간 유의적인 차이는 보이지 않았지만 T3 > T1 > T2 > C 순으로 높은 경향을 보였다. 경제적 조수익성은 T3 > T1 > T2 > C구순으로 나타 났다. 이상의 결과를 종합해 볼 때, 배합사료와 조사료 만 급여하는 체계(C) 보다는 홍삼박, 광물질 및 흑설탕을 첨가 급여(T1,T2, T3)하는 체계가 도체등급 및 수익성 증대에 유리하며, 특히 증체량, 육질등급 및 경제성을 고려하였을 때 T3구가 가장 좋은 급여체계로 나타났다.

본 연구는 Holstein 거세 비육우의 성장 단계에 따른 채식 및 반추행동에 미치는 영향을 규명하기 위하여 실시하였다. 실험에 사용한 공시 Holstein 거세우는 육성 단계(T1), 비육전기 단계(T2) 그리고 비육 후기 단계(T3)을 각각 3두씩, 총 9두를 3처리 3반복으로 배치하였다. 사료급여는 농후사료 체중의 1.62%, 조사료는 자유 채식시킨 후 육안으로 48시간 채식 행동관찰을 실시하였다. 체중대비 건물섭취량은 T1구(2.21%) 가장 높게 T3구 (1.39%)가 가장 낮게 나타났다. 채식시간은 T1, 반추시간은 T2 그리고 휴식시간은 T3 구에서 높게 나타났다(p<0.05, 0.01). 저작 시간은 T1 및 T2구에 비하여 T3구가 현저히 떨어지는 것으로 나타났다(p<0.01). 식괴수와 총 저작수는 다른 처리구에 비하여 T2구가 유의적으로 높게 나타났다(p<0.01). 그러나 식괴 당 저작수 및 분 당 식괴수는 T1구가 높게 나타났지만 상호 처리간 유의적인 수준은 나타나지 않았다. 음수 회수 및 배분 회수는 처리 구들 간에 유의적인 차이가 없었다. 그러나 배뇨 회수는 어린 단 계인 T1 구에서 유의적으로 높게 나타났다(p<0.01). 음수 시간, 배분시간, 배뇨시간은 T1 구에서 높게 나타났지만, 개체 차이가 심하여 유의적인 차이는 보이지 않았다. 이상의 결과를 종합하면 비육후기 단계(T3)는 육성(T1) 및 비육전기(T2)비하여 체중대비 채식량이 낮음에 따라 채식시간 반추시간은 줄어들고 휴식시간은 증가하는 것으로 나타났다. 또한 T3구는 총 식괴수 및 총 저작수도 떨어지는 것으로 나타났다.

Sperm cryopreservation is an important method of assisted reproductive techniques and storing genetic resources. It plays a vital role in genetic improvement, livestock industrial preservation of endangered species, and clinical practice. Consequently, the cryopreservation technique is well organized through various studies, especially on Korean native cattle (Hanwoo). However, the cryopreservation technique of Korean native brindled cattle, which is one of the native cattle species in Korea, is not well organized. Therefore, it is necessary to develop a Supplementary Table technique for the cryopreservation of Korean native brindled cattle. For this purpose, it is important to first evaluate the quality of the currently produced cryopreserved sperm of Korean native brindled cattle. In this study, we randomly selected 72 individual Korean native brindled cattle semen samples collected from 8 different region research centers and used them to evaluate sperm functions. We focused on the quality evaluation of cryopreserved Korean native brindled cattle semen following the measurement of motion kinematics, capacitation status, intracellular ATP level, sperm motility, and cell viability. Then, the values of each of the eight groups were derived from various sperm parameters of nine individual samples, including sperm motility, kinematics, cellular motility, and intracellular ATP levels, which were used to compare and evaluate sperm function. Overall, differences in various sperm parameters were observed between most of the research centers. Particularly, the deviations of motility and motion kinematics were high according to the sample. Therefore, we suggest that it is necessary to develop a standard method for the cryopreservation of Korean native brindled cattle semen. We also suggest the need for sperm quality evaluation of the cryopreserved semen of Korean native brindled cattle before using artificial insemination to attain a high fertility rate.

This study was conducted to evaluate the effect of rumen origin lactate-utilizing bacteria (LUB) as one of the potential treatments on subacute ruminal acidosis (SARA) during in vitro challenge compared to buffering agents (NaHCO3, sea minerals, MgO) and direct-fed microorganism (yeast). We hypothesized that rumen LUB (RLUB) could be a potential treatment to treat ruminal acidosis. The supplementation level of other treatments was determined by referring to previous studies in the literature. The 108 CFU/g freeze-dried RLUB isolated from Hanwoo cattle were compared with 0.1% NaHCO3, 0.8% of MgO, 0.5% sea mineral and 0.4% yeast during in vitro SARA challenge. Rumen fluid collected from one cannulated Holstein and one Hanwoo steer fed by maize silage was mixed with 0.5g feed consisted of 0.05g forage and 0.45g concentrate. These mixtures were incubated in triplication for 3, 6, 12 and 24h. After 6h of incubation, along with MgO and sea minerals, RLUB treatment showed higher (p<0.05) ㏗ values than control with no significant differences in total volatile fatty acid concentration. However, in the same period, the propionate concentration and A:P ratio were higher in RLUB than in the other treatment (p<0.05), which might alter the fermentation pattern. On the other hand, the RLUB treatment produced a higher (p<0.05) ammonia-N concentration. Based on these results, we can conclude that RLUB might have the potential to alleviate SARA. Nonetheless, further study on its mechanism in SARA is required, especially with live animals.

This study was conducted to evaluate the effects of different nitrogen levels on the yield and nutrient quality of a newly developed domestic hybrid of Teosinte, Geukdong 6〔Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis〕. The field experiment was conducted in a randomized block design with three replicates and consisted of four nitrogen (N) application rates, T1 (200 kg/ha), T2 (300 kg/ha), T3 (400 kg/ha), and T4 (500 kg/ha). No differences were found in plant length, leaf length, leaf width, leaf number, dead leaves, stem hardness, tiller number, and fresh yield (p>0.05). The T3 showed significantly greater dry matter yield at harvest (heading stage) compared to the other treatments (p<0.05). The crude protein content of T4 (10.49%) was higher than those of T1 and T2 (p<0.05). However, there was no significant difference between T4 (10.49%) and T3 (9.63%). The effects on crude fat, crude ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude fiber were not significant (p>0.05). The sugar content was higher in the T2 treatment than the other treatments (p<0.05). For Ca, T3 showed significantly greater content (p<0.05). However, no significant effects were found in the contents of Cu, Fe, K, Mg, Mn, Mo, and Zn (p>0.05). Na content was higher in order of T2 > T4 > T3 > T1 (p<0.05). Total mineral contents were not significantly different among the treatments (p>0.05). Given these results, we recommend the amount of nitrogen fertilization necessary for “Geukdong 6” to be around 400 kg per ha (T3), when considering, high fresh yield, dry matter yield, number of leaves and content of crude protein.

This study was carried out to investigate the growth characteristics, yield and feed value of Sorghum×Sudangrass hybrid for silage according to the drainage depths in the paddy field of lowland. The experimental design was arranged in a randomized block design with four treatments and three replication. The drainage depths of four treatments were 0cm, 20cm, 40cm and 60cm, respectively. Plant length, leaf length and leaf number were not significantly different, but leaf width increased as the higher the drainage depth(p<0.05). The number of dead leaf was higher in the order of 60cm > 0cm ≥ 40cm ≥ 20cm treatment(p<0.05). Green degree was higher in the order of 20cm > 40cm > 0cm > 60cm treatment(p<0.05). Stem diameter and stem hardness increased significantly as drainage depth increased from 0cm to 60cm(p<0.05). Also, fresh yield, dry matter yield and TDN yield increased as the higher the drainage depth(p<0.05). Crude protein and TDN content were the highest in 40cm treatment(p<0.05). Crude ash was higher in the order of 20cm > 40cm > 0cm > 60cm treatment(p<0.05). ADF and NDF content were the highest in 0cm treatment(p<0.05). Total mineral content was higher in the order of 20cm > 0cm > 40cm > 60cm(p<0.05). Free sugar content(fructose, glucose and sucrose) was the highest in 0cm treatment(p<0.05). Total amino acid(EAA+NEAA) was higher in 40cm than the other treatments(p<0.05). There is a difference in the content of ingredients(crude protein, TDN, mineral, free sugar and amino acid) according to the treatments. But considering dry matter yield and TDN yield, Sorghum×Sudangrass hybrid cultivation is advantageous to set the drainage depth of about 60cm in the paddy field of lowland.

This study was carried out to investigate the growth characteristics, yield, and chemical compositions of winter forage crops cultivated after rice harvest in the central inland region. The experimental design was arranged in a randomized block design with three replications. The treatments consisted of 4 species (Rye, Oat, Triticale and Italian ryegrass), and varieties were “Marton”, “Donghan”, “Shinyoung” and “Kowinnearly”, respectively. As a result, dry matter yield and TDN yield were higher in rye than in the other winter forage crops, and lowest in Oat(p<0.05). Crude protein and crude fat content were significantly higher in Italian ryegrass(p<0.05). However, Crude ash and ADF content did not show significant difference among winter forage crops. NDF content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05). TDN and total amino acid content (EAA+NEAA) were higher in order of winter forage crop with high crude protein content (Italian ryegrass > Oat > Triticale > Rye). Total mineral content was higher in order of Rye > Triticale > Oat > Italian ryegrass(p<0.05), and total free sugar content was higher in order of Oat > Italian Ryegrass > Triticale > Rye(p<0.05). Compared with the results above, Italian ryegrass and Oat are high in crude protein, TDN, amino acid and free sugar content. Rye and Triticale have the merit that feed value is decreased but high yield(dry matter and TDN yield) can be maintained. Therefore, it is advantageous to grow Rye and Triticale as winter forage crops after rice harvest in the central inland region.

This study was carried out to investigate the effects of seeding dates and cultivated period on the growth characteristics, dry matter yield and feed value of Teosinte new variety “Geukdong 6”〔Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis〕for feed. The experimental design was arranged in a randomized block design with three replications. Treatments consisted of five time seeding dates, 10 May(T1), 25 May(T2), 10 June(T3), 25 June(T4) and 10 July(T5), and same time harvesting, 22 October. Therefore, growing period were 164 days(T1), 149 days(T2), 134 days(T3), 119 days(T4) and 103 days(T5), respectively. In growth stage at harvest time, T1, T2, T3, T4 and T5 were early flowering, end heading, middle heading, early heading and early heading stage, respectively. Plant length and dead leaf were highest in T1, but leaf width and number of leaf were highest in T2 than others (p<0.05). Leaf length, stem diameter and number of tiller were not significantly different among the treatments (p<0.05). Stem hardness was higher in order of T1(2.0)> T2(1.9) > T3=T4(1.7) > T5(1.2kg/cm2). Fresh yield and dry matter yield showed significantly higher as the sowing time was faster and the cultivation period was longer (T1 > T2 > T3 > T4 > T5, p<0.05). Crude protein, crude fat and TDN content were highest in T5, but ADF and NDF content were highest in T1 than others (p<0.05). T1, T2 and T3 showed significantly higher crude protein yield compared to T4 and T5 (p<0.05). Total digestible nutrients yield were higher in order of T1 > T2 > T3 > T4 > T5 (p<0.05), and relative feed value were higher in order of T5 > T4 > T3 > T2 > T1 (p<0.05). Based on the above results, seeding dates could be recommended from the early May to the late May, and it is efficient that the cultivation period is over 150 days for dry matter yield, crude protein yield and total digestible nutrients yield.

Virtual reality is one of the most popular technologies in the fourth industrial revolution. We suggest that virtual training using virtual reality would be more effective than existing training. Development of virtual trolley simulation content was done through Unreal Engine. Through the production of virtual reality content, we had developed a more advanced simulation development environment.

This study investigated the comparative growth characteristics, yield, chemical compositions, and nutritive yield of Italian ryegrass cultivars harvested in a paddy field. The experimental design was a randomized block design with three replicates. The treatments consisted of seven Italian ryegrass cultivars (Kogreen, Kowinmaster, Hwasan 101, Kowinnearly, Kospeed, Sahalie select, and Strike). The planting date was October 21, 2009 and the sample harvest was occurred on May 21, 2010. Dry matter yield was higher in Kowinnerary and Kogreen than in the other varieties (p<0.05). Crude protein and ether extract contents were highest in Hwasan 101 and lowest in Kospeed. The content of total digestible nutrients did not differ among the Italian ryegrass varieties. The amino acid contents were in the order Hwasan 101 > Strike > Kowinnearly > Kogreen > Sahalie select > Kospeed > Kowinnearly (p<0.05). Fructose content was highest in Kospeed, while glucose and sucrose contents were highest in Hwasan 101. The results of this study indicate that Kowinnearly, in terms of dry matter yield, and Hwasan 101, in terms of feed value (chemical composition and free sugar content), may be recommended for forage production in a paddy field.

The present study investigated the effect of enzyme inclusion on silage quality using meta-analysis tool. A total of 16 research papers reporting the effect of enzyme application on silage quality were employed in the meta-analysis of this study. Mixed model for integrating quantitative results from multiple studies was used first to calculate the predicted error of each study. Individual error from the estimated model was the applied into standard deviation of each study to calculate the mean difference. Finally, summary effect was determined using standard mean difference (SMD) and inversed variance weighting. Mixed model analysis and SMD analysis showed the same effect patterns in all analysis items. Enzyme inclusion in silage significantly (p < 0.05) altered all silage quality characteristics investigated compared to control when enzyme was not included. Our results showed that enzyme treatment increased dry matter content, preserved crude protein effectively, and elevated water soluble carbohydrate content. However, the pH value, acetic acid, propionic acid, neutral detergent fiber, and acid detergent fiber contents in silage with enzyme inclusion were lower than those of the control.

This study evaluated the effects of TMC (trace mineral-fortified microbial culture) supplementation on growth performance, carcass characteristics, and meat quality parameters of Hanwoo steers during the last 4 months of finishing period. The TMC was a combination of 0.4% trace minerals, 20.0% Na-bentonite, and 79.6% feedstuffs, which was inoculated with a mixed microbial culture (Enterobacter ludwigii, Bacillus cereus, B. subtilis, Lactobacillus plantarum, and Saccharomyces cerevisiae). Twenty-four steers were blocked by initial BW (634 ± 16 kg) and randomly allocated to one of two treatments (control vs. 3.3% TMC). The effect of TMC supplementation on the growth performance was not significant. There was no incidence of urolithiasis in TMCfed steers. However 3 out 12 steers (25%) fed the control diet were observed to have urinary calculi. The carcass yield and meat quality parameters were not affected by TMC supplementation, however marbling score was increased in TMC-fed steers (P = 0.08). There was no effect of TMC treatment on the chemical composition of longissimus dorsi muscle (LM). The TMC supplementation increased concentrations of manganese (P < 0.01), cobalt (P = 0.02), iron, and copper (P = 0.06) in LM. In conclusion, TMC treatment did not negatively affect growth performance and meat quality parameters, and positively affected the trace minerals profile of LM.

This study was conducted to evaluate growth characteristics, yield and feed value of newly developed domestic teosinte (Geukdong 6) compared to widely cultivated silage corn hybrid (31N27) and sorghum × sudangrass hybrid (P877F). Geukdong 6, a new single cross (Teosinte × Suwon 19) variety, is a teosinte (Zea may L. ssp. maxicana) developed by the Geukdong 6 of agricultural company corporation (GACC) in An-dong province. This field experiment was designed in a randomized block design with three replications and consisted of silage corn (T1), sorghum x sudangrass hybrid (T2) and Geukdong 6 (T3). Leaf length, number of leaves, number of tillers and fresh yield of T3 were higher than that of T1 and T2 (p<0.05). Dry matter yield of T3 was lower than that of T1 (p<0.05). In particular, T3 (0.1 kg/cm2) showed to be very tender compared to other T1 (0.5 kg/cm2) and T2 (0.5 kg/cm2), (p<0.05). The crude protein content of T3 as 8.25% was higher than that of T2 (5.90%), (p<0.05). However, there was no significant differences between T1 (8.14%). The brix degree of T2 was higher than that of T1 and T3 (p<0.05). T3 showed higher content of total minerals compared to T1 and T2 (p<0.05). The relative palatability of T3 was higher than T1 and T2 (p<0.05), when fed to deer as soiling crop. However, it showed a lower than T1 in Korean native cattle and korean native goats. The relative palatability of the silage shown in order of T1> T3> T2 in korean native cattle, spotted deer, and korean native goats. As mentioned above the results, Geukdong 6 (T3) could be recommended as using of soiling and lab silage, when high fresh yield, tender stem, number of many leaf, content of high crude protein, and palatability higher than sorghum × sudangrass hybrid were considered.

The objective of this study was to determine the effect of different pregnancy stages on the eating and ruminating behavior of Hanwoo heifers (Bos taurus coreanae). A total of twelve Hanwoo heifers were divided into four groups depending on pregnancy stage: C: before pregnancy, 9 months old, T1: 3 months pregnant, 16 months old, T2: 6 months pregnant, 19 months old, T3: 9 months pregnant, 22 months old (3 replicates per group). Concentrate of 1% of the body weight was fed to them. Roughage was provided at ad libitum to all treatments. Our results revealed that the intake of roughage was the highest in the T3 group (at 3.68 kg) and the lowest in the C group (at 1.02 kg, p<0.05). Total intake in the order from high to low was: T3 > T2 > T1 > C (p<0.05). The rate of dry matter intake compared to body weight (DMI/BW, %) from high to low order was: T3 > T2 > T1 > C (p<0.05). Eating time, ruminating time, and chewing time were the highest in T3 group (p<0.05). Resting time was the highest in C group (p<0.05). The number of bolus and the number of total chews were significantly higher in T3 compared to those in other groups. However, ruminating time per bolus was not significantly difference among groups. The number of chews per bolus from high to low order was: C > T1 > T3 > T2 (p<0.05). The number of bolus per minute was not significantly different among groups. The number of drinking and the number of defecating were significantly higher in C and T1, respectively (p<0.05). However, the number of urinating had no effect according to pregnancy stage. Eating rate and chewing efficiency were significantly higher (p<0.05) in accordance with pregnancy stage (T3 > T2 > T1 > C). Ruminating efficiency was significantly (p<0.05) higher T1 than that in other groups.

본 실험은 논 토양에서 사일리지용 수수 × 수수 교잡종 재배시 화학비료와 발효 돈분 액비 혼용 시용이 생육특성 및 영양성분에 미치는 영향을 조사하고자 실시하였다. 실 험설계는 화학비료 100% 처리구 (C), 화학비료 70% + 돈분 액비 30% 처리구 (T1), 화학비료 50% + 돈분액비 50% 처 리구 (T2), 화학비료 30% + 돈분액비 70% 처리구 (T3) 그리 고 돈분액비 100% 처리구 (T4)로 한, 5처리 3반복 난괴법 으로 배치하였다. 이때 돈분 액비 시용은 질소량만을 기준 으로 하였다 (150 kg/ha). 초장, 엽장, 엽폭 그리고 경의 굵 기는 T4구가 유의적으로 작게 나타났다 (p<0.05). 경의 경 도는 돈분액비 시용 비율 낮고 화학비료 시용 비율이 높을 수록 유의적으로 증가하였다 (p<0.05). 생초수량은 T2구가 높았던 반면 T3구가 낮게 나타났다 (p<0.05). 그러나 건물 수량 및 TDN 수량은 처리구들 간에 유의적인 차이를 보이 지 않았다. 조단백질 함량은 T1구가 다른 구에 비하여 높 게 나타났다 (p<0.05). 조지방 함량은 T1, T2, T3 그리고 T4 처리구들 간에는 유의적인 차이가 없었지만, C구와는 유의적인 차이를 보였다 (p<0.05). NDF와 조섬유 함량은 각각 T3구와 C구에서 높게 나타났다 (p<0.05). 그러나 ADF 함량은 처리구들 사이에 유의적인 차이를 보이지 않았다. 총무기물 함량은 T1 > T2 > T4 > T3 > C 구 순으로 높게 나 타났다 (p<0.05). 유리당 함량은 T1과 C구가 다른 처리구에 비하여 유의적으로 높게 나타났다 (p<0.05). 이상의 결과를 종합해 볼 때, 돈분액비 시용은 화학비료에 비하여 수량성, 영양성분에 크게 떨어지지 않기 때문에 사일지용 수수 × 수수 교잡종 재배시 돈분액비와 화학비료를 혼용 시용 하 거나 돈분액비 만 시용하여도 큰 무리가 없는 것으로 판단된다.

This study aimed to investigate the effects of Dandelion (Taraxzcum coreanum) supplementation on milk yield, milk composition and blood characteristics in lactating dairy cows. Eight lactating dairy cows were divided into two groups (control: TMR supplementation, treatment: TMR with Dandelion supplementation). The milk yield, milk fat, lactose, solids not fat (SNF) and somatic cells counts (SCC) were not significantly different between the control group and the treatment group, whereas milk protein, milk urea nitrogen (MUN) and free fat acid (FFA) were significantly higher in the treatment group compared to the control (p<0.05). The blood components of the treatment group were compared with those of the control group and only aspartate aminotransferase (AST) appeared significantly high (p<0.05). The other blood components were not significantly different in the two groups. Blood corpuscle components in the groups were not significantly different. Especially, all blood corpuscle components in the treatment group were within the normal range. However, the white blood cells (WBC), lymphocytes (LYM) and hematocrits (HCT) in the control group exceeded the normal range. Based on the above results, the addition of Dandelion to feed increased milk protein, MUN and FFA, but did not significantly affect the composition of the blood and corpuscle in Holstein milking cows.