To investigate the oviposition preference and development of azuki bean weevil (Callosobruchus chinensis L.), the following six different leguminous seeds were used in this study: red bean (Vigna angularis (Willd.) Ohwi & Ohashi), black soybean (Glycine max (L.) Merr.), soybean (Glycine max (L.) Merr.), seoritae (Glycine max (L.) Merr.), small black bean (Rhynchosia nulubilis) and kidney bean (Phaseolus vulgaris var. humilis Alef.). In the study of oviposition preference, the numbers of eggs per leguminous seed on red bean, black soybean, soybean, seoritae and small black bean were 1.23, 0.61, 0.69, 1.05 and 1.13, respectively. The maximum daily number of eggs was observed at 48 hours and the minimum was at 96 hours. According to each host leguminous seed, developmental time for each host seed was different. The shortest adult emergence time was on red bean (25.27 days). The other five leguminous seeds increased or doubled the adult emergence time. Adult emergence rates feeding on red bean, seoritae, black soybean, soybean, small black bean were 83.33%, 28.23%, 27.87%, 20.44%, and 11.59%, respectively. Emergence rate on red bean was four times higher than the rate on other seeds. The longevity of emerged female adults was almost all longer than that of males. The male adults weighed the lowest of feeding on small black bean. Female adults weighed the lowest of feeding on soybean. Adult weights were the heaviest for both males and females feeding on red bean. As a result, hosts of azuki bean weevil could decrease oviposition rate, emergence rate, adult longevity, and adult weight but increase emergence time. Especially in kidney bean, adult was not completely emerged. No eggs were laid. These results suggest that there might be emergence inhibitors in kidney bean. These imformation might be used to control damages caused by azuki bean weevils.

In the present study, lichen(Parmelia sp.) extract showed insecticidal activity against Aedes albopictus, and the effect of growth inhibiting activity was investigated. Acetone and methanol extracts of the lichen against larvae of Aedes albopictus were showed high insecticidal activities in low lethal concentration. 50% lethal concentration of the acetone extractof the lichen is 0.13% and the 50% lethal concentration of methanol extract of lichen is 0.15% respectively. This experiment that used acetone and methanol extracts of the lichen were observed for 24, 48 and 72 hours. As a result, the higher concentration and the longer exposure time is increased mortality against Aedes albopictus. Pupation time took more time as the higher concentration of acetone extract of lichen. Consequently, the lichen extract is effect in inhibiting the growth of Aedes albopictus larvae. In this experiment indicates that lichen extract has activity against Aedes albopictus and is available as the natural insecticide.

The White backed planthopper (WBPH), Sogatella furcifera (Horvath) is one of the serious insect pests in rice growing region in Asia. When rice is attacked by the insect it releases secondary metabolites for self-defense. In this study, we identified WBPH-mediated compounds from a cross ‘Cheongcheongbyeo/Nagdongbyeo’ doubled haploid (CNDH). The compounds were located in chromosome. Leaves and stem of CNDH lines were infected by 2∼3 insta of 3 weeks WBPH and samples were extracted by 90% methanol. Extracted compounds were analyzed through HPLC. TLC was used in separating the target compounds. QTL analysis of compound was done using winQTLcart 2.5 program. Chrysoeriol was highly contained in Cheongcheongbyeo. QTL location is found on chromosome by winQTLcart 2.5. QTL analcited with compound7 was detected on chromosome 4, 7 and 12. qFla4 was detected on chromosome 4 in RM280-RM6909 at LOD 3.5 with 30% of variation. qFla7 was detected on chromosome 7 in RM248-RM1134 with LOD 3.0 with 30% of variation. qFla12 was detected on chromosome 12 in RM1226-RM12 with LOD 2.7 with 40% of variation. Cochlioquinone was detected on chromosome8, qFla8 in RM23230-RM3689 with LOD 2.5 with 30% of variation. Chrysoeriol and Cochlioquinone separated to condition of (Chloroform: Methanol:1-Butanol:Water=4:5:6:4). Separated compounds were analyzed by LC/MS and NMR. These results, investigation is being done to ditermine how the secondary metabolites come lead to pathways of genes and its effect on WBPH relation.

This experiment conducted to identify the changes of the response when white-back planthopper (WBPH, Sogatella furcifera), were inoculated in 10 days rice leaves after germination. We confirmed the difference between inoculated and uninfected plants by in the different time period (1day, 1 week, 2 weeks, and 3 weeks after inoculation). Breeding rice and WBPH maintained at 26∼28℃ with 60 % humidity. 3 leaves plants (TN1, Cheongcheong, and Nagdong) were inoculated with 2∼3 instars WBPH. Harvested rice plant samples were completely dried in dark condition and then samples were completely immersed in a solution of methanol for 3 days under darkness. Dissolving in water and then de-fatted three times with hexane. 100 ppm samples were applied to HPLC, eluting with acetonitrile and 0.1 % acetic acid by C18 (5ph column Agilant) and detected at 254 nm. We confirmed the difference of peak using LC/MS/MS (API-2000). The results showed that three weeks from the day of inoculation was increased at the molecular weight 118.1, 264.2 and 364.2.