This study was conducted to investigate the effectof supplemental lighting intensity (SLI) on the shoot growthand flower quality of Rosa hybrida ‘Pink Bell’ in winter sea-son. High pressure sodium lamps (HPS) which were set upat 1.4m height above the planting beds were adjusted forthe SLI treatments: no supplemental lighting (control), pho-tosynthetically active radiation (PAR) 30, 50, 70, and 90µmol•m−2•s−1 in 20h daylength (17:00 to 22:00 and 02:00to 09:00). Shoot growth including shoot elongation, shootweight, and flowering speed was promoted quantitatively asSLI increased. But the flower quality such as petal num-bers, petal pigmentation, and biomass distribution to thepetals was declined at PAR 90, even though the proper SLIfor rose production was generally known in the range ofPAR 90 to 120. In this experiment PAR 90 treatment madethe surface of petals heated near 30oC which was about10oC higher than the control group without supplementallighting. The surface temperature of the petals was continu-ously rising because the distance to lamps from floral budsbecame increasingly short as the shoots grew in localgreenhouse facilities. The heat stress by HPS lampscaused petal discoloration and over-consumption of assimi-lation products due to excessive respiration and water lossin petals. Thus, it is necessary to prevent heat injury byswitching light intensity depending on shoot growth consid-ering local greenhouse structure.

In this work, we report in-situ observations of changes in catalyst morphology, and of growth termination of individual carbon nanotubes (CNTs), by complete loss of the catalyst particle attached to it. The observations strongly support the growth-termination mechanism of CNT forests or carpets by dynamic morphological evolution of catalyst particles induced by Ostwald ripening, and sub-surface diffusion. We show that in the tip-growth mode, as well as in the base-growth mode, the growth termination of CNT by dissolution of catalyst particles is plausible. This may allow the growth termination mechanism by evolution of catalyst morphology to be applicable to not only CNT forest growth, but also to other growth methods (for example, floating-catalyst chemical vapor deposition), which do not use any supporting layer or substrate beneath a catalyst layer.

장수명 핵분열생성물인 79Se와 99Tc는 자연수 중에서 용해도가 클 뿐더러 음이온으로 존재하여 방사성폐 기물 처분장에서 주요 관심핵종들로 고려되고 있다. 본 연구에서는 KURT 지하수의 다양한 pH와 산화-환 원 조건에서 셀레늄과 테크네튬의 Solubility Limiting Solid Phase (SLSP)로 알려진 FeSe2와 TcO2의 용해 도를 측정하였다. 또한, 지화학코드를 이용하여 실험과 유사조건에서 이들의 용해도와 주요 화학종을 계 산하였다. 실험 및 계산으로부터 pH 8∼9.5와 Eh=-0.3∼-0.4 V 조건에서 FeSe2의 용해도는 1x10-6 mol/L 이하이며, 주 용해 화학종은 HSe-로 판단된다. TcO2의 경우는 pH 6∼9.5와 Eh<-0.1 V 영역에서 용해도와 주 용해 화학종이 각각 5x10-8∼1x10-9 mol/L와 TcO(OH)2로 나타났지만, Eh=-0.35 V조건에서는 주 용해화학종이 pH가 10.5∼12와 12이상에서 각각 TcO(OH)3 -와 TcO4 -로 계산되었다.

Field survey was conducted to determine the differences of spider community between traditional farming and environmental friendly farming at 17 localities, Korea in 2008. In guild structure, The occupancy rate of pest group, general group and natural enemy group was 37.3%, 41.0% and 21.6% respectively in environmental friendly farming, and 36.9%, 35.6% and 27.5% respectively in traditional farming. Among natural enemy group, parasitoids and predators were 15.8% and 84.2% respectively in environmental friendly farming, and 28.1% and 71.9% respectively in traditional farming. Among natural enemy group, rice field spiders were 74.4% and 62.2% in environmental friendly farming and traditional farming respectively. Among predators, rice field spiders were 86.7% in environmental friendly farming and 84.9% in traditional farming. Rice field spider community comprises 28 species of 24 genera belonging to 10 families and 30 species of 24 genera belonging to 10 families in environmental friendly farming and traditional farming respectively. Dominant families were Lycosidae, Linyphiidae and Tetragnathidae and dominant species were Pirata subpiraticus, Ummeliata insecticeps, Tetragnatha maxillosa, Gnathonarium dentatum and Packygnatha clercki in both agricultural practices. The ratio of hunting spiders and webbing spiders was similar between agricultural practices.

L-trans-pyrrolidine-2,4-dicarboxylate (PDC) is a potent inhibitor of glutamate transporters. In our current study, we investigated whether the neuronal death induced by PDC involves mechanisms other than excitotoxicity in mixed mouse cortical cultures. Cortical cultures at 13-14 days in vitro were used and cell death was assessed by measuring the lactate dehydrogenase efflux into bathing media. Glutamate and PDC both induced neuronal death in a concentration-dependent manner but the neurotoxic effects of glutamate were found to be more potent than those of PDC. Treatment with 10, 100 and 200 M PDC equally potentiated 50 M glutamate-induced neuronal death. The neuronal death induced by 75 M glutamate was almost abolished by treatment with the NMDA antagonists, MK-801 and AP-5, but was unaffected by NBQX (an AMPA antagonist), trolox (antioxidant), BDNF or ZVAD-FMK (a pan-caspase inhibitor). However, the neuronal death induced by 200 M PDC was partially but significantly attenuated by single treatments with MK-801, AP-5, trolox, BDNF or ZVAD-FMK but not NBQX. Combined treatments with MK-801 plus trolox, MK-801 plus ZVAD-FMK or MK-801 plus BDNF almost abolished neuronal death, whereas combined treatments with trolox plus ZVADFMK, trolox plus BDNF or ZVAD-FMK plus BDNF did not enhance the inhibitory action of any single treatment with these drugs. These results demonstrate that the neuronal death induced by PDC involves not only in the excitotoxicity induced by the accumulation of glutamate but also the oxidative stress induced by free radical generation. This suggests that apoptotic neuronal death plays a role in PDCinduced oxidative neuronal injury.

Human recombinant IL-32 induces the production of large amounts of several proinflammatory cytokines and chemokines, even in macrophage cell lines by activation of NF-κB and MAPK. The ability of IL-32 to potentiate inflammation is not the sole action of this cytokine. IL- 32 stimulates prostaglandin (PG)E2 in human PBMCs, which are pivotal in inflammation. The development of recombinant protein consisting of IL-32 is of great medical and industrial importance. Agrobacterium strains GV3101 harboring pCAMBIA1304 include IL- 32 vectors were used to infect the mushroom fruiting body pieces. For transformation experiments, fruiting body gill tissue pieces were vacuum infiltrated with the suspension of induced bacteria until the air had been completely purged. Mushroom mycelium appeared at the margins of the tissue pieces after 9 to 14 days on selection medium with hygromycin at 50㎍/㎖. Transformants PCR analyses confirmed that the IL-32 gene was into the genome of Pleurotus eryngii. The present results demonstrating the usefulness of the transgenic technique s in the genetic manipulation and improvement of mushroom.

Human GH (hGH) has been available over 40 years for the treatment of children with GH deficiency. Human growth hormone (hGH) is mainly produced in the somatotrophic cells of the pituitary in brain and is the product of the GH-N gene. Among the edible mushrooms, the king oyster mushroom (Pleurotus eryngii ) is one of the most popular mushrooms in Asia, Europe and North America. The increasing popularity of P. eryngii among consumers is due to its flavor, texture and shelf life. We report a modified Agrobacteriummediated method for the efficient transformation of hGH2 in Pleurotus eryngii . The binary vector pCAMBIA1304 was used for the initial transformation and detected by GUS and GFP. Infiltrated samples transformed with pCAMBIA1304 showed a wider GUS response than the co-cultivated in the 50㎍/㎖ hygromycin selection medium. This transformation technique offers new prospects for the production of useful protein in the genetically improved mushroom.

To reveal the relationship between the changes in the growth and photo- synthesis induced by low dose radiation, red pepper (Capsicum annuum L.) plants were serially irradiated three times with gamma rays of 0.5, 1, 2, 3, and 4 Gy. The plant growth was mo

본 논문 에서는 강구조물의 설 계를 위한 가상하중 소 성활절 해석기볍이 연구되 었 다. 구조풀 의 기하학적 인 불완전성 올 가상하중 기법으로 고 려하였다. 본 해 석 기볍을 통하여 구조물 의 거동과 하증 지지 능력 을 직접적인 방법으로 예측할 수 있다. 즉 본 기법 은 강구조 설 계 에서 전통적으로 사용되고 있 는 유효길이 인 지 (K-fac tor) 의 계산 및 각 부재의 강도계산을 필요로 하지 않으므로써 다음 세대의 설 계기볍이라고 할 수 있다. 본 기볍 에 의하여 예측된 강도와 변위는 정확한 해라고 알려진 Plastic-Zone 해석 결 과와 비교 검증하였다， 본 기볍의 해석 및 설계 세 부 지침과 순서 를 제시하였으며， 본 해석기볍 및 AISC-LRFD 방법에 의하여 결 정된 부재크기 를 비교하였다. 본 해석기법은 실 제 설계에 효율 적 으로 사용될 수 있을 것으로 사료된다.

To derive the distributions of electron density, temperature and gas-phase metal abundances within the Orion Nebula, we have performed a non-LTE analysis to the radio observations of hydrogen recombination lines and continuum flux over the frequency range from 0.1GHz to 100GHz. We have explicitly included the thermal balance condition in our analysis, hence our derived distributions have their internal consistencies. This enables us to derive the radial distribution of Oxygen and Nitrogen. The gas-phase concentrations of these cooling agents show about the solar values at the very central part of the nebula, then, decrease slowly outward, and finally become about one quarter of the solar values in the outer extended envelope. Such an outward decrease is interpreted as an outward increase of dust concentrations in the Orion.

수문모형의 매개변수 추정에 필요한 유량 관측 자료의 수집은 시·공간적으로 제한이 있어 우리나라도 아직 상당수의 미계측유역이 존재하며, 이를 보완하고자 주변 유역의 정보를 활용하는 지역화 방법들이 연구되어 왔다. 그러나 지역적 특성이나 기후 조건에 따라 지역화 방법의 결과가 상이하여 어느 지역에 어떠한 지역화 방법이 가장 우수하다고 판단하기 어렵다. 본 연구에서는 보편적으로 사용되는 지역화 방법인 지역회귀모형의 설명 변수에 공간근접모형으로 추정한 수문모형의 매개변수를 추가하여 회귀모형의 적합성을 향상시켰으며, 이를 하이브리드 지역화모형이라 정의하고 기존 방법들과 비교하였다. 계측유역으로는 관측 자료가 충분한 남한의 37개 유역을 선정하였고, 수문모형은 개념적 수문모형인 GR4J를 사용하였으며, 계측유역에 대한 수문모형의 매개변수 산정은 Shuffled complex evolution 알고리즘을 사용하였다. 유역 특성변수들 간 다중공선성을 고려하기 위해 Variation inflation factor를 사용하였고, Stepwise regression을 통해 회귀모형의 최적 설명변수를 선택하였다. 통계 값을 통해 모형의 적합성을 비교한 결과, 하이브리드 지역화모형에서 가장 작은 RMSE 값을 나타내었으며, 유역별 모의 값의 변동성이 줄어들어 결과의 불확실성 또한 낮아짐을 확인할 수 있었다. 따라서 하이브리드 모형이 미계측유역의 유출량 산정을 위한 하나의 대안이 될 수 있음을 확인하였다.

Background : Insect pests damages are increasing on the field of Lycium chinense under eco-friendly organic management and especially, pest damage of Gelechiidae (Ilseopsis parki Povolny) is serious. Currently, various eco-friendly organic materials are registered, but insecticidal activity against Gelechiidae was not verified. Therefore, this experiment was carried out to select eco-friendly organic materials showing insecticidal activity in Gelechiidae. Methods and Results : To test insecticidal activity, the Gelechiidae lavas were collected from the field and Nine eco-friendly organic materials were collected from the market. method. The insecticidal activity assay method was, putting Gelechiidae lavas on the 90mm petridish then water-diluted eco-friendly organic materials were sprayed onto the laver. Result of insecticidal activity was observed with a microscope 10 minutes, 2 hours and 18 hours after the treatment. Microscopic observation results, insecticidal activity was shown only in 5 out of 9 materials. Conclusion : As a result, Insecticidal activity against Gelechiidae was not effective in all tested materials, but the 5 eco-friendly organic materials showed the same effect in repeated experiments. In this study, we selected 5 materials out of 9 eco-friendly organic materials against Gelechiidae, then future studies are planned to select the most suitable materials by field testing the 5 eco-friendly organic materials.

This study was carried out to examine the effects of NaOCl, sucrose, and BA concentration as pretreatment solution on quality and vase life of cut flowers in Dendranthema grandiflorum ‘Jinba’. Flower diameter, fresh weight, and vase life in control and 0 mg･L -1 NaOCl treatments decreased, and the treatment with 100~200 mg･L -1 NaOCl was more effective in the quality and vase life. In pretreatment with 2.5% sucrose solution, flower diameter and fresh weight decreased and vase life was shortest due to the rapid leaf wilting. However, pretreatment with 0.1% sucrose solution increased the flower diameter and fresh weight, and showed the longest vase life. When more than 80 mg･L -1 BA was treated with pretreatment solution, flower diameter and fresh weight decreased, and vase life was shortened. With pretreatment of 20 mg･L -1 BA, the flower diameter was bigger than in the other treatments, but it was no effect on fresh weight and vase life. Therefore, it was suggested that pretreatment solution mixed with 200 mg･L -1 NaOCl, 0.1% sucrose, and 20 mg･L -1 BA was the most effective for the quality and vase life of cut flowers in standard chrysanthemum ‘Jinba’.