Inhibition of Rho-associated coiled coil-containing kinase (ROCK) has been reported to promote differentiation of neuronal cells. Here, we examined the effect of Y-27632, a ROCK inhibitor, on the outgrowth of neurites in PC12 cells. Y-27632 caused a rapid induction of neurite outgrowth in PC12 cells in a time-dependent manner. The neurite outgrowth, triggered by Y-27632, was accompanied by Rac1 activation, and was attenuated by Rac1 inhibitor NSC23766, in a concentration-dependent manner. Y-27632 also induced an increase in the production of reactive oxygen species (ROS). Pretreatment with N-acetylcysteine, an ROS scavenger, inhibited the ROS generation and neurite outgrowth in response to Y-27632. These results indicate that the activation of Rac1 and the generation of ROS contribute to the neurite outgrowth triggered by Y-27632 in PC12 cells.

A new graft cactus (Gymnocalycium schickendantzii × G. mihanovichii) cultivar “Geoseong” was developed from a interspecific crossing between a green G. schickendantzii and G. mihanovichii line “950222” by in vitro grafting and line selection at National H

A new graft cactus (Gymnocalycium mihanovichii) cultivar “Suhong” was developed from a cross between G. mihanovichii cv. “Seolhong” and a line “940623” by in vitro grafting and line selection at National Horticultural Research Institute, Rural Development

A new graft cactus (Gymnocalycium mihanovichii) cultivar “Hwiseong” was developed from a cross between G. mihanovichii “Orenge” and a line “943011” by in vitro grafting and line selection at National Horticultural Research Institute, Rural Development Adm

A new graft cactus (Gymnocalycium mihanovichii) cultivar “Heukhong” was developed from a crossing “941101” with mixed pollens of 10 unknown cultivars of G. mihanovichii by in vitro grafting and line selection at National Horticultural Research Institute,

A new graft cactus (Gymnocalycium mihanovichii) cultivar ‘Suhwang’ was developed from a crossing ‘9502165’ with mixed pollens of 10 unknown cultivars of G. mihanovichii by in vitro grafting and line selection at National Horticultural Research Institute,