식용곤충은 미래식량 자원으로써 우수한 가치를 지니고 있어 해외에서는 사육자동화, IoT 및 AI 기술적용, 수직재배시스템 구축 등 많은 연구가 진행되고 있지만 국내에서는 대규모 사육농가나 곤충스마트팜 기술개발 이 부족하여 이를 위한 AI/빅데이터 인프라 구축이 시급한 실정이다. 학습용 인공지능 데이터는 식용곤충으로 활용되고 있는 장수풍뎅이, 흰점박이꽃무지, 갈색거저리, 백강잠, 메뚜기, 풀무치의 생애 주기별 총 6종의 RGB 사진데이터와 분광이미지 데이터 408,000장을 구축하였으며 온도, 습도, CO,, 암모니아, 조도, 수분 등 환경 데이 터 200,000세트를 수집하였다. 수집된 데이터는 원시데이터 수집, 원천데이터 가공, 라벨링 데이터 결합, 가공데 이터 검수 등을 통해 만들어졌으며 관련 데이터는 AI Hub(www.aihub.or.kr)에서 다운받을 수 있다. 확보된 식용곤 충 6종의 데이터는 곤충 종별 성장단계, 환경 변수에 따른 최적의 사육환경 조성, 생산시기 예측, 스마트대량사육 시스템 개발, 제품 가공시 추적이력제 도입, 식용곤충 스마트팜 기술 개발 및 연구 등 다양한 분야에 활용될 수 있을 것으로 예상된다.

Truck no-show behavior has posed significant disruptions to the planning and execution of port operations. By delving into the key factors that contribute to truck appointment no-shows and proactively predicting such behavior, it becomes possible to make preemptive adjustments to port operation plans, thereby enhancing overall operational efficiency. Considering the data imbalance and the impact of accuracy for each decision tree on the performance of the random forest model, a model based on the Borderline Synthetic Minority Over-Sampling Technique and Weighted Random Forest (BSMOTE-WRF) is proposed to predict truck appointment no-shows and explore the relationship between truck appointment no-shows and factors such as weather conditions, appointment time slot, the number of truck appointments, and traffic conditions. In order to illustrate the effectiveness of the proposed model, the experiments were conducted with the available dataset from the Tianjin Port Second Container Terminal. It is demonstrated that the prediction accuracy of BSMOTE-WRF model is improved by 4%-5% compared with logistic regression, random forest, and support vector machines. Importance ranking of factors affecting truck no-show indicate that (1) The number of truck appointments during specific time slots have the highest impact on truck no-show behavior, and the congestion coefficient has the secondhighest impact on truck no-show behavior and its influence is also significant; (2) Compared to the number of truck appointments and congestion coefficient, the impact of severe weather on truck no-show behavior is relatively low, but it still has some influence; (3) Although the impact of appointment time slots is lower than other influencing factors, the influence of specific time slots on truck no-show behavior should not be overlooked. The BSMOTE-WRF model effectively analyzes the influencing factors and predicts truck no-show behavior in appointment-based systems.

The radiological characterization of SSCs (Structure, Systems and Components) plays one of the most important role for the decommissioning of KORI Unit-1 during the preparation periods. Generally, a regulatory body and laws relating to the decommissioning focus on the separation and appropriate disposal or storage of radiological waste including ILW (intermediate level waste), LLW (low level waste), VLLW (very low level waste) and CW (clearance waste), aligned with their contamination characteristics. The result of the preliminary radiological characterization of KORI Unit-1 indicated that, apart from neutron activated the RV (reactor vessel), RVI (reactor vessel internals), and BS (biological shielding concrete), the majorities of contamination were sorted to be less than LLW. Radiological contamination can be evaluated into two methods. Due to the difficulties of directly measuring contamination on the interior surfaces of the pipe, called CRUD, the assessment was implemented by modeling method, that is measuring contamination on the exterior surfaces of the pipes and calculating relative factors such as thickness and size. This indirect method may be affected by the surrounding radiation distribution, and only a few gamma nuclides can be measured. Therefore, it has limitation in terms of providing detailed nuclide information. Especially, α and β nuclides can only be estimated roughly by scaling factors, comparing their relative ratios with the existing gamma results. To overcome the limitation of indirect measurement, a destructive sampling method has been employed to assess the contamination of the systems and component. Samples are physically taken some parts of the systems or components and subsequently analyzed in the laboratory to evaluate detailed nuclides and total contamination. For the characterization of KORI Unit-1, we conducted the radiation measurement on the exterior surfaces of components using portable instruments (Eberline E-600 SPA3, Thermo G20-10, Thermo G10, Thermo FH40TG) at BR (boron recycle system) and SP (containment spray system) in primary system. Based on these results, the ProUCL program was employed to determine the destructive sample collection quantities based on statistical approach. The total of 5 and 8 destructive sample quantities were decided by program and successfully collected from the BR and SP systems, respectively. Samples were moved to laboratory and analyzed for the detail nuclide characteristics. The outcomes of this study are expected to serve as valuable information for estimating the types and quantities of radiological waste generated by decommissioning of KORI Unit-1.

Commercial condom advertisements usually emphasize the sexual pleasure of branded products, which leads to controversial public views. Some people agree that commercial condom advertisements can also benefit public health, whereas others disapprove of such commercial condom advertisements because their contents are usually offensive, low-tasted, and pornographic. Despite controversy over commercial condom advertising, we know little about the spillover effect of commercial condom advertisement. On one hand, sexual-related content in the commercial condom advertisement may have an arousal effect. That is, it can evoke sexual arousal, leading to more sex intercourses. More frequent sex behaviors, especially casual sex behaviors, may then lead to a risk of contracting sexually transmitted diseases (STDs). On the other hand, commercial condom advertisements may have an educational effect. That is, it can persuade people to use condoms, helping people get in the habit of using condoms, thus reducing the STD trends. In the short term when condom commercials are aired, the arousal effect and educating effect coexists, which motivates the net short-term effect as an open empirical question. In the long-term when the condom commercial no longer aired, the educating effect remains, which decreases the STD trends.

Governments around the world are enacting laws mandating explainable traceability when using AI(Artificial Intelligence) to solve real-world problems. HAI(Human-Centric Artificial Intelligence) is an approach that induces human decision-making through Human-AI collaboration. This research presents a case study that implements the Human-AI collaboration to achieve explainable traceability in governmental data analysis. The Human-AI collaboration explored in this study performs AI inferences for generating labels, followed by AI interpretation to make results more explainable and traceable. The study utilized an example dataset from the Ministry of Oceans and Fisheries to reproduce the Human-AI collaboration process used in actual policy-making, in which the Ministry of Science and ICT utilized R&D PIE(R&D Platform for Investment and Evaluation) to build a government investment portfolio.

This study assessed the utility of netted melon ‘Top Earl’s’ and cantaloupe melon ‘Alex’ as functional fruits by analysing their moisture content, vitreous sugar, folic acid, citric acid, and beta-carotene levels. High-performance liquid chromatography (HPLC) was used to analyse the free sugar, folic acid, citric acid, and beta-carotene levels. The moisture content was not significantly different between ‘Top Earl’s’ and ‘Alex.’ The glucose, sucrose, and fructose contents were three, two, and one-and-a-half fold higher in ‘Alex’ than in ‘Top Earl’s.’ Moreover, citric acid was approximately three times higher in ‘Alex’ than that in Top Earl’s.’ However, the folic acid content was higher in ‘Top ‘Earl’s’ than ‘Alex,’ and the amount was 124 μg / 100 g FW and 112 μg / 100 g FW respectively. ‘Beta-carotene was undetectable in ‘Top Earl’s,’ whereas it was 1000 μg / 100 g FW in ‘Alex.’ β-carotene, a substance that is converted in the body into vitamin A and acts as an antioxidant, is an important component in healthy food. These results suggested that the cantaloupe melon ‘Alex’ has a higher free sugar content and functional ingredients, such as antioxidants, including citric acid and beta carotene, than the netted melon ‘Top Earl’s.’

Explainable AI (XAI) is an approach that leverages artificial intelligence to support human decision-making. Recently, governments of several countries including Korea are attempting objective evidence-based analyses of R&D investments with returns by analyzing quantitative data. Over the past decade, governments have invested in relevant researches, allowing government officials to gain insights to help them evaluate past performances and discuss future policy directions. Compared to the size that has not been used yet, the utilization of the text information (accumulated in national DBs) so far is low level. The current study utilizes a text mining strategy for monitoring innovations along with a case study of smart-farms in the Honam region.

다양한 동물 모델이 인간 질병, 의약품의 효능 및 작용 메커니즘을 연구하는 데 사용되고 있다. Zebrafish(Danio rerio)는 여러 가지 장점이 있어 인간 질병에 대한 중개 연구의 모델로 점점 더 폭넓게 활용되고 있다. 본 논문은 Pubmed, Google Scholar, Scopus에서 2020년 12월까지 최근 10년간 zebrafish 모델, 천연물(한약), in vivo 스크리닝의 키워드를 사용하여 저널에 게재된 논문을 검토하여 필 요한 정보를 얻었다. 이 리뷰에서 우리는 천연물(한약) 연구에 대한 다양한 제브라피쉬 질병 모델의 최 근 경향에 대해 논의하였다. 특히, 암, 안질환, 혈관 질환, 당뇨병 및 합병증, 피부질환에 중점을 두었고, zebrafish 배아를 사용하여 이들 질병에 대한 의약품의 분자 작용 메커니즘에 관해 언급하였다. Zebrafish는 실험실에서 임상 연구까지의 격차를 줄이는 데 중추적 역할을 할 수 있는 중요한 동물 모 델이다. Zebrafish는 의약품이나 화장품 개발, 질병의 병인론을 이해하기 위해 사용되고, 이로 인해 생의 학 연구에서 설치류의 사용을 줄이는 데 크게 기여하고 있다.

Acute myocardial infarction (AMI) is considered the major cause of mortality in the world. Tremendous animal studies are performed to develop novel therapeutics, and this study aimed to induce porcine myocardial infarction model by using polyethylene terephthalate (PET). Coronary guidewire was placed in left anterior descending artery (LAD). The balloon angioplasty catheter was inserted at the back of the PET. The balloon catheter was carefully pushed forward, until the balloon marker was located in mid-LAD. Coronary angiography was performed pre- and post-occlusion at 28 days by C-arm. Histologic analysis of heart tissue was performed 28 days after inducing AMI. Thirty three pigs were anesthetized and underwent percutaneous coronary catheterization. All pigs were successfully embolized in mid-LAD by PET. Fifteen pigs died due to ventricular fibrillation during post-anesthetic recovery time, and overall experiment mortality was 45.5%. In 2,3,5- triphenyl tetrazolium chloride staining, gross finding of the ischemic heart lesion showed firm and white area of infarction associated with the apex and left ventricular posterior wall. Infarct on H&E-stained sections demonstrated a region without myocytes and rich with cardiomyocyte with atypical nuclei. Successful induction of AMI by using PET may provide the pathophysiological information of ischemic heart disease and improvement of therapy development for AMI.

Alcohol intake is known to affect various organs in the human body, causing reduction of salivation in the oral cavity. Hypo-salivation effect of alcohol is a common feature, but the mechanism in salivary glands is still poorly studied. Therefore, in this study, the changes in salivary secretion and water channel protein (aquaporin5, AQP5) in salivary glands of mice were investigated after ethanol administration. Animals were divided in to 4 groups with the control, 4 g/kg ethanol, 8 g/kg ethanol and 16 g/kg ethanol administration groups. One hour after ethanol administration, saliva was collected from the oral cavity, and the animals were killed and parotid and submandibular glands were extracted to analyze the histopathology, AQP5 immunihistochemistry and AQP5 protein level. According to the results, the salivation rate decreased irrespective of the ethanol dose in mice, and viscosities increased with increase in ethanol dose. However, there were no pathological changes in parotid and submandibular glands due to ethanol administration. Expression of AQP5 in parotid and submandibular glands decreased with increase ethanol administration These results indicate that the reduction of salivary secretion due to acute alcohol intake is closely related to decrease of the water channel protein such as AQP5 in parotid glands and submandibular glands, rather than the damage of salivary glands.

Stored grain pests can cause reduction of grain quantity, quality, commercial value and germination rate. Susceptibility of three fumigants, methyl bromide, ethyl formate and phosphine, were assessed on Tribolium castaneum, which is an important stored grain pest. On susceptible insects, LCT50 of phosphine was 0.654mg h/L for egg, 0.127mg h/L for late larvae, 0.105mg h/L for pupae and 0.048mg h/L for adult stage, respectively. LCT50 of methyl bromide was 33.193mg h/L for egg, 14.585mg h/L for late larvae, 8.616mg h/L for pupae and 11.967mg h/L for adult stage, respectively. LCT50 of ethyl formate were 25.165mg h/L for egg, 80.912mg h/L for late larvae, 176.326mg h/L for pupae and 68.578mg h/L for adult stage, respectively. On resistant insects, LCT50 of phosphine were 82.325mg h/L for egg, 33.315mg h/L for late larvae, 73.546mg h/L for pupae and 55.707mg h/L for adult stage, respectively. LCT50 of methyl bromide were 19.250mg h/L for egg, 43.413mg h/L for late larvae, 76.842mg h/L for pupae and 19.387mg h/L for adult stage, respectively. LCT50 of ethyl formate were 87.552mg h/L for egg, 113.457mg h/L for late larvae, 200.122mg h/L for pupae and 85.394mg h/L for adult stage, respectively.

본 연구는 고농도 CO2 환경에 순응한 호접란 유묘의 CO2 교환 및 생장 반응을 조사하기 위해 실시하였다. 환경 조절이 가능한 식물생장상에서 6주령 호접란 ‘만천홍’을 27주 동안 야간에 각각 400 ± 100, 900 ± 100, 1500 ± 100, 2100 ± 100μmol CO2·mol-1 농도로 유지해주었다. 처리에 앞서 발달해 있었던 최상위 성숙엽으로 측정한 야간 중 CO2 흡수량은 900μmol CO2·mol-1 처리구에서 가장 높았고, 이어 2100, 1500, 400μmol CO2·mol-1 순으로 높았다. 그러나 처리 7주 이후 새로 발달한 성숙엽에서 측정한 결과, 2100μmol CO2·mol-1 처리구가 야간 중 가장 높은 CO2 흡수량을 보였으며, CO2의 흡수량은 처리 CO2 농도가 높아질수록 증가하는 경향을 보였다. 생장 반응에서는 최상위 성숙엽의 엽장, 엽폭 및 생체량은 900, 1500, 2100μmol CO2·mol-1 등 고농도 처리구에서 감소하였으나, 신엽 발달은 고농도 CO2 처리 하에서 촉진되었다. 이러한 결과들은 호접란 유묘의 높은 CO2 환경에 대한 적응이 CO2 흡수와 신엽 발달을 증진시킬 수 있다는 것을 보여준다. 그러나 900μmol CO2·mol-1 이상의 고농도에서는 잎의 생장과 생체량이 다소 줄어드는 경향이 있어 이에 대한 원인구명 등 추가적인 연구가 필요하다.

Oocyte is the central factor in the bi-directional communication axis in the ovarian follicles. It controls the cumulus or granulosa cells to perform functions which are beneficial for its own development via secreting paracrine growth factors, including GDF9 and BMP15. The aim of this study was to investigate whether the recombinant GDF9 and BMP15 are able to promote meiotic resumption and cumulus expansion of canine COCs during IVM, as well as to demonstrate the actions of GDF9 and BMP15 in regulating the expression of connexin transcripts in the ovarian granulosa cells. As results, GDF9 and BMP15 significantly improved the meiotic resumption rate and cumulus expansion by activating ERK1/2 signaling. Treatments with GDF9 significantly improved the expression of CyclinB1 but inhibited the expression of Cx43 transcripts. In addition, cumulus expansion genes (MAPK1, Ptgs2, Tnfaip6 and Ptx3) were differentially improved by GDF9 and BMP15. In the ovarian granulosa cells, GDF9 suppressed the expression of Cx43 transcripts by binding ALK4/5/7 receptors and activation Smad2/3 signaling, whereas, BMP15 stimulated the expression of Cx43 transcripts by binding ALK2/3/6 receptors and activating Smad1/5/8 signaling. In conclusion, by regulating functions of granulosa/cumulus cells, oocyte has the potential to enhance the growth and maturation of itself.

Somatic cell nuclear transfer (SCNT) is the technique which generates embryos by transferring diploid nucleus into an enucleated oocyte, it has produced specific animals successfully in a variety of species. However, the developmental capacity of SCNT embryos is still relatively lower than that of embryos produced in vivo. Oocyte is a kind of lipid rich cells, its quality limits the efficiency of embryo production. L-carnitine is a co-enzyme facilitating the transportation of long chain fatty acids across the inner mitochondria membrane where fatty acids are used for generating adenosine triphosphate (ATP) via beta-oxidation. It also has antioxidant actions which may protect mitochondrial membranes and DNA against damage induced by reactive oxygen species (ROS). Whether L-carnitine is functional in bovine SCNT embryos are unknown. Therefore, the objective of this study was to examine the effects of L-carnitine on oocyte maturation and developmental competence of subsequent SCNT embryos. L-carnitine was supplemented during IVM, then intracellular ROS and GSH levels, mitochondrial activity, gene expression of COCs were analyzed at the end of IVM. SCNT embryos were produced subsequently, apoptosis detection and gene expression evaluation were performed in blastocysts. In the results, treatments with 1.5 mM and 3 mM L-carnitine significantly improved maturation rates (P<0.05). Treatments with 3 mM L-carnitine effectively induced improvement in nuclear maturation, intracellular GSH levels and mitochondrial activity, as well as a reduction in intracellular ROS levels (P<0.05). mRNA levels of CPT1A, ACAA1, ACAA2, AREG, EREG, SOD1, GPX4, GLUT1 and CDC2 transcripts were effectively up-regulated by 3 mM L-carnitine treatments (P < 0.05). Similarly, 3mM L-carnitine induced an increase in blastocyst developmental rates and an improvement in blastocyst quality (P<0.05). Our study indicates that L-carnitine treatment during IVM improves oocyte nuclear maturation and subsequent SCNT embryo development.

The mesenchymal stem cells (MSC) has been investigated as a source of stem cell therapy to replace and treat damaged cells. Human endometrial epithelial and stromal cells was isolated from hysterectomy tissue and the direct evidence of stem/progenitor cells in the human endometrium was identified. Endometrium derived stem cells (EnMSCs) are known to have a high proliferative ability, genetic stability, lack of tumorigenicity and low immnunogenicity during long-term cultivation. Here, we aimed to identify MSC in canine endometrium and characterize its potential to differentiate into decidua cells. EnMSCs were isolated from thrown-away spayed uterus of adult canine depending on their estrus cycle, and identified by flow cytometry, immunocytochemistry and flow cytometry with MSC specific markers. We then characterized the ability of EnMSCs by the doubling-time analysis, colony-forming units and MSC differentiation assays. Isolated EnMSCs expressed stem cell specific genes (Sox2, Oct4, Nanog, MCAM, Endoglin, Susd2 and IGTB) and MSC surface markers (CD90, CD44 and CD117). EnMSCs are also differentiated into adipogenic, osteogenic and chondrogenic cells morphologically under modified conditions with the expression of lineage specific genetic markers. EnMSCs showed higher proliferation ability than canine amniotic fluid derived MSCs which were used as a positive control. EnMSCs were cultured at low density (10, 20, cells/cm2) and initiated to form small colonies of loosely-arranged cells and gradually formed large colonies of densely-packed cells which underwent self-renewal with high proliferative potential which is similar to the clonogenicity feature of human endometrium-derived stem cells. EnMSCs were then induced to differentiate into decidua cells with 0.5 mM dbcAMP. After 14 days, EnMSCs changed their morphology into the elongated and rounded shape. The induced decidual cells expressed PRL and IGFBP1 which are typically expressed in decidua cells. In conclusion, we successfully isolated and characterized MSC in the canine endometrium which differentiated into decidua cells. These results showed that endometrium may be a promising source of stem cells, and furthermore raise the possibility of canine EnMSCs as a novel hypothetical decidualisation model of infertility associated with decidualisation insufficiency and implantation failure.

Oocyte is the central factor in the bi-directional communication axis in the ovarian follicles. It controls the cumulus or granulosa cells to perform functions which are beneficial for its own development via secreting paracrine growth factors, including GDF9 and BMP15. The aim of this study was to investigate whether the recombinant GDF9 and BMP15 are able to promote meiotic resumption and cumulus expansion of canine COCs during IVM, as well as to demonstrate the actions of GDF9 and BMP15 in regulating the expression of connexin transcripts in the ovarian granulosa cells. As results, GDF9 and BMP15 significantly improved the meiotic resumption rate and cumulus expansion by activating ERK1/2 signaling. Treatments with GDF9 significantly improved the expression of CyclinB1 but inhibited the expression of Cx43 transcripts. In addition, cumulus expansion genes (MAPK1, Ptgs2, Tnfaip6 and Ptx3) were differentially improved by GDF9 and BMP15. In the ovarian granulosa cells, GDF9 suppressed the expression of Cx43 transcripts by binding ALK4/5/7 receptors and activation Smad2/3 signaling, whereas, BMP15 stimulated the expression of Cx43 transcripts by binding ALK2/3/6 receptors and activating Smad1/5/8 signaling. In conclusion, by regulating functions of granulosa/cumulus cells, oocyte has the potential to enhance the growth and maturation of itself.

Removal of phosphate from environmental water has become more important to prevent eutrophication. In the present study, sorption behavior of phosphate onto magnesite was investigated under different conditions. The optimum pH of phosphate adsorption was determined to be 6.0. The adsorption capacity was found to decrease with increasing temperature, which indicates that a low temperature was beneficial for phosphate adsorption. The sorption capacity for phosphate was found to be 10.2 mg/g at an initial concentration of 100 mg/L and a dose of 2 g/L. The first order kinetic equation and Freundlich isotherm model fit the data well. Phosphate adsorption on magnesite was explained by electrostatic attraction and weak physical interactions.

Somatic cell nuclear transfer (SCNT) has been considered for preserving genetically valuable or endangered animals. Sapsaree is a Natianal Monument in Korea to maintian a pure pedigree. The aim of this study was to produce azoospermia Sapsaree using SCNT and identify normal reproductive ability of cloned azoospermia Sapsaree. Ear skin biopsy was performed on a thirteen-year-old azoospermia Sapsaree and ear skin fibroblasts were isolated for SCNT as donor cells. The fibroblasts were injected into enucleated in vivo matured oocytes, the couplets were electrical fused by two pulse of direct current (55 V for 15 μs) using titanium and platinum fusion needle and activated by calcium ionophore. Cloned embryos were surgically transferred into oviducts of natuarally estrus cycle synchronized recipient dogs. The fusion rate of platinum needle was 70%, which was higher than those of titanium needle (64.1%). Developmental rate to the 8 cells and 10 cell stages was higher in platinum needle group (24% and 16%, respectively) than those of platinum needle group (14.8% and 3.1%, respectively). Total 35 SCNT embryos were transferred into oviducts of 3 recipient dogs and one recipient finally delivered a puppy by caesarean section. As results, this study demonstrated that platinum fusion needle could be successfully make the reconstructed embryos and improve the efficiency of canine SCNT. Cloning azoospermia Sapsaree may contribute to conserve genetically valuable and unique pedigree. And further study should be confirm whether cloned live dog is azoospermia.

Prolonged communication between oocytes and the surrounding somatic cells is one of the unique reproductive physiology in canine. Paracrine Kit ligand (KITL) signaling is a well-known communication between granulosa cells and the oocyte. KITL is a cytokine growth factor secreted by granulosa cells that signals via the c-kit receptor expressed by oocytes. Paracrine factors, including growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15), exert their effects by binding with the kinase receptors expressed on the granulosa cells. However, the regulations of GDF9 and BMP15 in the canine KITL expression are currently poorly understood. Therefore, we investigated the effects of GDF9 and BMP15 on the expression of KITL in canine ovarian granulosa cells in vitro. In Annexin V assay recombinant GDF9 and BMP15 did not induce apoptosis in the cultured ovarian granulosa cells. When treated, FSH significantly increased KITL expression, and hCG suppressed its expression. When both FSH and hCG were treated, the expression of KITL was affected by GDF9 and BMP15 in dose and time dependent manner in the luteal granulosa cells. GDF9 (10 ng/mL) significantly decreased KITL expression after12 h. BMP15 (10 ng/mL) significantly also decreased KITL expression after 24 h. Western blot and immunochemistry results indicate that GDF9 activated Smad2/3. After blocking ALK 4/5/7 receptors by SB, GDF9 failed to activate Smad2/3, also BMP15 did not activate Smad1/5/8 after blocking ALK 2/3/6 receptors by DM. So GDF9 exerts its effects via using ALK 4/5/7 receptors to activate SMAD2/3 signaling, and BMP15 binds ALK 2/3/6 receptors to activate SMAD1/5/8 signaling. The expression of KITL was not changed by SB or DM treatment. However, the effect of GDF9 and BMP15, which decreased the expression of KITL, was suppressed by SB or DM treatment. In conclusion, this study provides the first evidence that recombinant GDF9 and BMP15 decrease KITL expression in canine ovarian granulosa cells.