Mealworm(Tenebrio molitor) was added to the feed to improve the health of dogs. As the amount of mealworm powder added to dog feed increased from 2.5 to 7.5%, the contents of crude protein, crude fat, crude fiber and crude ash increased. In the old dogs, creatinine and BUN related to nephropathy, triglyceride related to hypertension, calcium related to osteoporosis, globulin related to immunity, and ALT related to hepatic function were in the normal range. Particularly, ALT showed a tendency to increase overall, but the numerical increase tended to decrease as the amount of mealworm powder added. In the young dogs, all investigative items such as BUN and creatine were in the normal range and ALT tended to decrease at 2.5～5.0% treatment of mealworm. As a result, it was shown that adding 2.5～5.0% of mealworm powder to feed effectively improved the hepatic function of old and young dogs.

Ochratoxin A, which is frequently detected in cereals and infant diets worldwidely, is a mycotoxin to damage mainly the kidney and liver. Because ochratoxin A is highly thermostable compound. it is necessary to study ways of reducing level of ochratoxin A by controling processing steps. However, food processes, including extrusion, expansion, roasting, and steam cooking, which are used in order to mitigate the contents of ochratoxin A, are known to produce polycyclic aromatic hydrocarbons, which are generated from radicals decomposed by pyrolysis. Therefore, this study analyzed the levels of 4 polycyclic aromatic hydrocarbons, benz (a) anthracene, chrysene, benzo (b) fluoranthene and benzo (a) pyrene in rice-based products made in high pressure and heating process. Rice samples were finely ground, and homogenized samples were alkaline treatement with 1 M KOH/EtOH and extracted with liquid-liquid extraction method using n-hexane. The extracted solution was pretreated with a silica cartridge. The purified solution was dried with nitrogen gas and dissolved in 1 mL of dichloromethane and injected into GC/MSD. We had overall recoveries for 4 polycyclic aromatic hydrocarbons spiked into rice samples ranging from 92.8 to 110.2%. The limit of quantitations of benz (a) anthracene, chrysene, benzo (b) fluoranthene and benzo (a) pyrene in rice-based product were 0.19 ng/g, 0.38 ng/g, 0.51 ng/g, and 0.31 ng/g, respectively. However, these 4 polycyclic aromatic hydrocarbons in all processed rice samples were not detected.