This study was conducted to collect the patents of microbiome-based treatment technology for pets. An electronic search for microbiome or probiotics in brain nervous system disease was studied using the WINTELIPS database. Patent Cooperation Treaty of Korea, Japan, the EU, the US, and China that were registered by October 31, 2022 were selected in this study. A total of 206 patents were included for final analysis. Since 2016, patent activity has shown an explosive increase in recent years. China is the leading market in this technology field, and Korea has the second-highest market share. To provide the groundwork for the next research and development, we examined the industrial trend of microbiome for brain nervous system diseases in this study using an analysis of patents that have been applied for and registered up to this point. Looking at the overall patent trends by year in the technology field related to treating of brain and nervous system diseases using the microbiome, there was a tendency to repeat increasing and decreasing trends. However, considering 2021 and 2022, which have undisclosed sections, it can be seen that patent activity has tended to increase explosively in recent years, starting in 2016. If related studies use the patent analysis data constructed in this way strategically, it is expected that it will lead to patent registration and the development of new products, ultimately contributing to the revitalization of the companion animal industry.

Collagen peptides have garnered significant attention as functional foods across multiple fields due to their capacity to regulate physiological and hormonal processes, offering numerous advantages. However, despite their broad range of applications, comprehensive research on the potential toxicity of these substances remains lacking. Therefore, this study sought to assess the acute oral toxicity of a collagen peptide derived from skate (Raja kenojei) skin (CPSS) in both rats and dogs. In the rat model, CPSS was orally administered at doses of 300 and 2,000 mg/kg to Sprague-Dawley rats. An escalating single-dose oral toxicity assessment at doses of 500, 1,000, and 2,000 mg/kg was carried out in beagle dogs with 3-day intervals between doses. Throughout the 14-day post-administration assessment period, clinical signs, mortality rates, changes in body weight, and necropsy observations were closely monitored. After oral administration, no signs of toxicity associated with CPSS were observed in either rats or dogs. Therefore, the oral LD50 (approximate lethal dose for 50% mortality) for CPSS in rats was determined to exceed 5,000 mg/kg, and the maximum tolerated dose for dogs was estimated to be above 2,000 mg/kg. Consequently, this study offers safety data on the use of CPSS in functional foods and medicinal applications.

Fermentation by Rhizopus spp. has been used as a traditional medicine for treating various inflammatory diseases. Allergic asthma is a chronic respiratory disease that is caused by an exaggerated immune response. This study was conducted to ascertain the anti-inflammatory effects of Rhizopus spp. fermentation extract (RU) on a mouse model of ovalbumin (OVA)-induced asthma. The animals were intraperitoneally injected OVA on day 1 and 7, followed by OVA intranasal inhalation on days 14 to 18. The animals were treated daily with RU (100 and 200 mg/kg) by oral gavage from day 18 to day 23. RU significantly decreased eosinophilia and the production of inflammatory cytokines and OVA specific immunoglobulin E in animals with asthma, along with reducing airway inflammation and mucus secretion in lung tissue. Histological changes in the lungs and levels of inflammatory mediators of allergic airway inflammation were evaluated. The regulatory effects of RU on type 2 helper T (Th2) cell activation were investigated. RU administration attenuated asthmatic changes such as inflammatory cell infiltration and mucus production, decreased the levels of Th2-related cytokines, and reduced Th2 cell activation. Administration of RU effectively reduced allergic responses in asthmatic mice, which is associated with regulating Th2 cell activation and differentiation. These results indicate that RU can attenuate the respiratory symptoms of asthma.

Developments in cancer therapies and diagnostic techniques have improved the long-term survival of cancer patients. Certain cancer treatments, such as radiotherapy, often harm normal tissue as well as the specifically targeted cancer cells. High doses of radiation induce bone loss. This study investigated the effects of pentoxifylline (PTX) on radiation-induced bone loss in C3H/HeN mice. C3H/HeN mice were divided into sham and irradiation (3 Gy, gamma-ray, IR) groups. The irradiated mice were treated for 12 weeks with vehicle, PTX (p.o.) or PTX (s.c.). Grip strength, uterus weight, serum alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) level were measured. Tibiae were analyzed using micro-computed tomography. There were no significant differences in the degree of grip strength, body weight and uterine weight between IR group and PTX-treated group. Treatment of PTX significantly preserved trabecular bone volume, trabecular number, trabecular separation and bone mineral density of proximal tibia metaphysic. The administration of PTX lowered serum TRAP in IR mice, suggesting that PTX can reduce the bone resorptive rate in mice. Our experimental data support the protective role of PTX against bone loss in irradiated mice. Based on the findings of this study, development of PTXbased treatments is anticipated to address bone loss after radiotherapy. Prospective dose escalation studies are required to determine the appropriate dosage of PTX.

Celecoxib, a cyclooxygenase (COX)-2 selective inhibitor, was approved as a non-steroidal anti-inflammatory drug (NSAID), and this therapeutic application has been expanded to several other diseases, including colon cancer. Notably, a treatment strategy combining the use of celecoxib and radiation therapy has been employed for improving the control of local cancers. In this study, we examined the effect of celecoxib on irradiation-induced intestinal damage. The twenty four mice (BALB/c) were divided into four groups; 1) sham-irradiated control group, 2) celecoxib-treated group, 3) irradiated group, and 4) celecoxib-treated irradiation group. Mice were orally administered celecoxib at a dose of 25 mg/kg in a 0.1 mL volume, daily for 4 days after irradiation exposure (10 Gy). Then, histological examinations of the jejunal villous height, crypt survival, and crypt size were performed. The expression of COX-2 after administration of celecoxib in irradiated mice was examined by employing immunohistochemistry, Western blotting, and qPCR analysis. The jejunal villi height and the crypt survival were reduced in the irradiation group compared with the sham-irradiated group. Celecoxib treatment in irradiation mice even more decreased those indicators. Crypt size was increased in the radiation group compared to the sham-irradiated control group, whereas the size was decreased in the celecoxibtreated irradiation group compared with the group exposed to the radiation injury. COX-2 expression was detected in the crypt of the small intestine, and COX-2 expression was increased in the crypt lesion following radiation exposure. However, COX-2 expression was reduced in the celecoxib-treated irradiation group. Therefore, in the present study, we confirmed that celecoxib treatment after irradiation aggravated the irradiation-induced intestinal damage. These results suggest that a caution need to be administered when celecoxib treatment is performed in combination with radiation therapy for cancer treatment.

Various viral and bacterial pathogens interact with environmental factors to cause diarrhea in piglets. Enterococcus spp. are Gram-positive anaerobic bacteria that are commonly found in the gastrointestinal tract of several animal species, including pigs. Enterococcus spp. have been reported to infect several animal species as a pathogen. However, gastrointestinal infection by Enterococcus hirae is rare in pigs; only a few cases have been reported worldwide. Four piglets with diarrhea were examined in the diagnostic laboratory of Optipharm Inc. (Cheongju, Korea). During the initial post-mortem examination, no disease lesions were observed. Upon microscopic examination, we found numerous Gram-positive cocci that were adhered to epithelial villi in the jejunum and ileum. However, the villi did not exhibit significant structural damage. Cultured bacteria were identified as E. hirae using the VITEK 2 system and polymerase chain reaction (PCR). Using PCR, we also confirmed that viruses and protozoa that can potentially infect piglet intestines were absent. In antibiotic susceptibility test, the bacteria were resistant to most types of antibiotics. This study presents rare cases of E. hirae infection of the piglet small intestine, which can occur in association with diarrhea possibly by the continuous use of antibiotics.

Osteoporosis is a major worldwide public health problem that poses a great economic burden to society. Puerariae Radix, the dried root of Pueraria lobata (Wild.) Ohwi, has been widely used in Asia. This study investigated the effects of Puerariae Radix (PR) on bone loss in ovariectomized (OVX) mice. C3H/HeN mice (10 weeks old) were divided into sham and OVX groups. The OVX mice were treated with vehicle, 17β-estradiol (E2), PR (oral administration, 250 mg/ kg/day) or PR (intraperitoneal administration, 50 mg/ kg/every other day) for 6 weeks. Grip strength, uterus weight, serum alkaline phosphatase (ALP), estradiol concentration and osteoclast surface levels were measured. Tibiae were analyzed using microcomputed tomography. There were no significant differences in the degree of grip strength, body weight and uterine weight between OVX group and PR-treated group. As compared with the OVX group, the serum estradiol levels were significantly increased in the PR-treated group. PR (i.p.) significantly preserved trabecular bone volume, trabecular bone number, structure model index and bone mineral density of proximal tibiae metaphysic. The administration of PR lowered serum ALP and osteoclast surface levels in OVX mice, suggesting that PR can reduce the bone turnover rate in mice. The results indicate that the supply of PR can prevent OVX-induced bone loss in mice.

본 연구는 폴리에틸렌 중합에 이용되는 Ziegler-Natta 촉매와는 다른 Indene및 Cyclopent adien을 기반으로 하는 다양한 메탈로센 촉매를 사용하여 폴리에틸렌 왁스를 중합하고 중합한 폴리에틸렌 왁스 의 특성에 대해 분석하고 평가하였다. 폴리에틸렌 왁스 중합은 각 다른 구조의 리간드를 포함하는 메탈로 센 촉매에 대하여 중합온도, 연쇄이동제로 사용되는 수소와 에틸렌가스의 비율을 조정하여 다양한 조건하에서 중합을 시도 하였으며 그에 따른 분자량과 분자량 분포, 촉매 수율을 비교 분석하였다. 결과적으로 본 연구를 통하여 저분자량을 가지며 좁은 분자량 분포를 가지기에 적합한 메탈로센 촉매의 구조를 제안하였으며 이상적인 폴리에틸렌 왁스를 중합 할 수 있었다.

Volunteer is defined as a person who offer service for social welfare work without pay voluntarily and people who recognize the importance of volunteer activity and engage in volunteer work in government offices and non-governmental organizations. However, the disaster protection for the volunteers is still out of the law’s boundary. The volunteer in volunteer organizations, social welfare organization as well as teenage volunteer are now protected in nation through private health insurance but its coverage is limited and the guarantee level is not sufficient to protect volunteer. If volunteers cannot be protected from industrial accidents or paid proper compensation to realize their human dignity by assuring a basic right to live, it leads to serious loss personally and socially. If the volunteers are protected by occupational health and safety insurance that functions as social insurance and the industrial accident risk gets removed, it will contribute to invigorate the volunteer activities and thus, legal improvement and institutional strategy must be required so that volunteers enjoy various insurance benefits in Industrial Accident Insurance.

An evidence suggests that even low-dose irradiation can lead to progressive cognitive decline as well as memory deficits in both humans and experimental animals in part due to hippocampal dysfunction. To determine whether or not green tea (GT) and epigallocatechin gallate (EGCG) could attenuate memory impairment as well as suppress hippocampal neurogenesis, passive avoidance and object recognition memory test as well as TUNEL assay and immunohistochemical detection with markers of neurogenesis (Ki-67 and doublecortin (DCX)) were performed using adult mice treated with relatively low-dose gamma irradiation (2.0 Gy). GT was administered intraperitonially at a dosage of 50 mg/kg of body weight at 36 and 12 hr pre-irradiation and at 30 minutes post-irradiation, or orally at a dosage of 250 mg/kg of body weight/day for 7 days before autopsy. EGCG (25 mg/kg of body weight) was administered intraperitonially at 36 and 12 hr pre-irradiation and at 30 minutes post-irradiation. In the passive avoidance and object recognition memory test, mice trained for 1 day after acute irradiation (2 Gy) showed significant memory deficits compared with sham controls. The number of TUNEL-positive apoptotic nuclei in the dentate gyrus increased by 12 h after irradiation. In addition, the numbers of Ki-67- and DCX-positive cells significantly decreased. GT treatment prior to irradiation attenuated memory defects, blocked apoptotic death, as well as reduced the number of DCX-positive cells. Therefore, GT may attenuate memory defects in adult mice exposed to a relatively low dose of radiation possibly by inhibiting the detrimental effects of irradiation on hippocampal neurogenesis.

Panax ginseng, also known as Korean ginseng, has long been used as a broad tonic in Oriental medicine to augment vitality, health, and longevity, particularly in older people. This study investigated the effects of Korean red ginseng (RG) on bone loss in ovariectomized (OVX) mice. C3H/HeN mice (10-weeks-old) were divided into sham and OVX groups. OVX mice were treated with vehicle, 17β-estradiol (E2), RG (oral administration, 250 mg/kg/day), or RG (intraperitoneal administration, 50 mg/kg/every other day) for 6 weeks. Serum E2 concentration and alkaline phosphatase (ALP) activity were measured. Tibiae were analyzed using microcomputed tomography. Biomechanical properties and osteoclast surface level were measured. There was no significant difference in the degree of grip strength, body weight, uterine weight, mechanical property, tibiae length, or tibiae weight between the OVX and RG-treated groups. Compared with the OVX group, the serum ALP level was significantly lower in the RG-treated groups. Serum E2 levels and osteoclast surface levels did not change between the OVX and RG-treated groups. RG could not preserve trabecular bone volume, trabecular bone number, trabecular separation, trabecular thickness, structure model index, or bone mineral density of the proximal tibiae metaphysic. In conclusion, there was no definite effect of RG on OVX-induced bone loss in C3H/HeN mice.

Recently, since the development of internet and online space, people experience new communication space and games different from traditional ones. People share their experiment of games and item in the online space with internet. This paper proposes the design of the MMORPG item’s pricing decision system. First, the on-going “chatting” in the game chatting system is collected by chatting API. This collected chatting is the information including terms of game item. Then, extracted terms of game item are classified on the basis of collective intelligence model and generate a table of weight. At last, this system can recommend suitable MMORPG item to users with the obtained weight table storing as ontology.

To evaluate the acute to chronic effects of crude oil exposure on hematological and blood biochemical toxicities, Sprague-Dawley rats were given oral doses of 0, 50, or 100 mg/kg BW/day of Iranian heavy crude oil for a period of four weeks. In the acute phase of exposure (one day after four weeks of oil treatment), decreases in weight of thymus, serum level of interferon gamma (IFN-γ), superoxide dismutase (SOD), and catalase activities in liver or kidney, and increase in weight of adrenal gland occurred after oral administration of crude oil. In body weight, histopathological examination, hematological and blood biochemical analyses in the acute phase of exposure, no significant differences were observed among the experimental groups. In the subchronic and chronic phase of exposure (two months and six months after four weeks of oil treatment), the changes of biomarkers were normalized, except the indicators of oxidative stress. Our findings showed that the bioassay on the indicators of oxidative stress is a sensitive method for determining exposure to crude oil in rats.

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

웹 기반의 온라인 교육은 강의실에서 수업하는 면대면 강의의 부족한 점을 보완하는 가장 효과적인 방법 중의 하나이다. 웹을 이용한 학습은 자기주도적인 학습이 가능하고 학습자와 교수자 간의 상호 보완적인 학습이 이루어질 수 있다는 장점이 있기 때문이다. 이러한 온라인 교육을 효과적으로 지원하기 위해서는 학습관리 또는 콘텐츠관리 기능을 제공해주는 학습관리시스템이 필요하다. 학습자들에게 양질의 온라인 콘텐츠를 제공하고자 하는 교사들을 위해 개발된 학습관리시스템 중 하나가 Moodle LMS이다. Moodle LMS는 소스가 공개되어 있어 누구나 필요한 사람은 소스를 개선, 확장, 활용할 수 있다. 따라서, 본 논문은 Moodle LMS를 활용하여 교수자가 교양 사이버강좌를 쉽게 개설하고 관리․운영할 수 있는 학습관리시스템을 확장하여 활용하는데 목적이 있으며 이를 확장하면 온라인 게임도 관리․운영할 수 있다.

소듐냉각 고속로 (SFR) 핵연료 피복관 후보재료로 고려되고 있는 중형 규모의 HT9 단조품 소재에 대한 금속조직학적 영향을 고찰하였다. 시험 재료는 유도가열법을 이용하여 1.1톤 규모의 잉곳으로 성형한 후, 1170℃에서 고온 단조 및 공랭을 통하여 160mm 직경 및 7000mm 길이를 갖는 단조품으로 가공하여 반 경방향으로 미세조직의 변화를 관찰하였다. 시험 결과 시험 재료는 페라이트-마르텐사이트 조직을 보였 으며 합금 조성에 의하여 2~3%의 델타 페라이트 (delta ferrite)를 가짐과 동시에 반경방향의 냉각속도 차 이에 의하여 최대 15%의 변태 페라이트 (transformed ferrite)를 함유함이 관찰되었다. 냉각곡선의 모델 링과 시간-온도-변태 (TTT) 선도를 이용한 민감도 분석을 통하여 단조품의 직경을 120mm로 줄였을 경우 중심부의 변태 페라이트 형성을 억제할 수 있음을 제시하였다.

This study was conducted in order to evaluate the anti-inflammatory activities of Phellinus linteus (PL) water extract against carrageenan- and cotton pellet-induced acute and chronic inflammation in rats. Animals were divided into four experimental groups for conduct of acute and chronic inflammations studies: the vehicle control group, positive control group (aminopyrine 100 mg/kg), PL 10 mg/kg group, and PL 20 mg/kg group. Oral administration of PL water extract has dose-dependent anti-inflammatory effects against both acute and chronic inflammation models. In carrageenan-induced paw edema, significant inhibition was observed at 0.5 and 1 h in the PL 10 mg/kg group and at 0.5, 1, 2, 3, 4, and 6 h in the PL 20 mg/kg group. In cotton pellet-induced granuolma formation, PL water extract at 10 and 20 mg/kg/day also showed significant inhibition in dry weights of granuloma. Results of the free radical scavenging assay showed that PL has a dose-dependent scavenging activity against 2,2-diphenyl-1-picrylhydrazyl free radicals. The results obtained in this study indicate that this plant material may be used beneficially as an anti-inflammatory agent having antioxidant action.

본 연구는 2007년도 조사된 국가산림자원조사 자료를 이용하여 충북지방에 분포하는 주요 수종에 대하여 평균 연륜생장량과 연륜생장량에 영향을 미치는 인자들과의 상관관계를 분석하고자 하였다. 충북지역에서 조사된 고정표본점은 총 56개소로 다양한 수종이 분포하였으며 그 중 개체수 분포 비율이 높은 10개 수종에 대하여 총 800본의 연륜생장량이 조사된 목편 자료를 대상으로 분석하였다. 충북지방에서 평균 연륜생장량이 가장 높은 수종은 아까시나무(2.30mm/yr)로 나타났고, 그 다음으로는 졸참나무(2.27mm/yr)>산벚나무(1.98mm/yr)>일본 잎갈나무(1.98mm/yr) 순으로 나타났다. 대부분 주요 수종들의 경우, 영급과 임분밀도가 증가함에 따라 평균 연륜생장량은 감소하는 경향으로 나타났다.

There have been significant changes to family values and the types of families have diversi-fied. This phenomenon gave way to the rise of the lower fertility and aging society, and could also cause disorganization or crisis of family in the future. It has now become necessary to build family-friendly values through the new education envi-ronment, Web 2.0. Social Network Service (SNS), the main idea of this study, represents one type of the new environment. It is a vital and well-matched concept with family-friendly val-ues. The paradigm shift in education has features such as interaction, relationship and com-munication. The purpose of this study was to suggest feasibility of Social Network Service for forming family-friendly values. To verify the feasibility, four family websites in Korea were examined with the research method of SWOT Analysis. To find strategies, SWOT factors were identified as follows; 1) Strength factors of Social Network Service for family-friendly values formation: free ser-vice, diverse functions, and global interactivity through language service. 2) Weakness factors: unstable, virtual disorder resulting from too many functions, and lack of user directedness. 3) Opportunity factors: increasing needs of family value education and improvement to mul-ticultural society. 4) Threat factors: digital literacy divide between old and new generation and decreasing time to communicate with family. In this study, the following seven strategies were extracted through SWOT analysis: 1) Practice educational paradigm shift: Web 2.0 2) Change educational settings in multicultural society 3) Challenge media convergence 4) Represent ability of ubiquitous learning 5) Promote interactivity across all age groups, taking into account life cycle 6) Present user-directed interfaces 7) Conduct instructional contents or curriculum with blended learning at K-12 Keywords: Family friendly values, Web 2.0, Learning 2.0, SWOT Analysis

본 연구에서는 2005년부터 2013년까지 운용되었던 국내 연안여객선의 항로를 사회네트워크 분석 방법을 통해 파악하였다. 우선 국 내 연안여객 항로의 네트워크 특성을 살펴본 결과 네트워크 복잡계 특성인 멱함수의 법칙을 따르는 Scale-Free 형태를 보이고 있음을 확인하 였다. 본 연구에서는 사회 네트워크 분석의 중요 척도라고 할 수 있는 중심성을 바탕으로 살펴보았으며, 연도별 연결정도 중심성의 변화추세, 기존의 정량적인 방법과의 비교를 통한 양의 상관관계 확인, 마지막으로 연결정도 중심성과 매개 중심성간의 관계를 분석하여 두 가지의 척도를 기준으로 우선시되는 항구를 순위별로 확인할 수 있었다. 본 연구는 연안여객 항로를 하나의 네트워크로 간주하고 분석하여 유용한 결과를 제시하였다. 앞선 결과들을 바탕으로 국내 연안여객 항로의 개발 등 발전방향을 설정할 시 보다 효과적으로 진행이 가능할 것으로 보인다. 또 한 공항의 중심성 분석, 주변국의 외교 관계 분석 등 다양한 분야에서 사회네트워크 분석 방법을 활용한다면 유용한 결과를 산출하는데 효과적 일 것으로 판단된다.