In this study, we have determined mitochondrial genome of Matsucoccus thunbergianae isolated in Korea. The circular mitogenome of M. thunbergianae is 15,406 bp including 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNAs. AT ratio is 78.2%. Maximum-likelihood and Bayesian inference phylogenetic trees show that M. thunbergianae is clustered with M. matsumurae, and family Margarodidae is clustered with family Pseudococcidae with enough supportive values.

본 연구에서는 한국산 미기록속인 고수씨살이좀벌속(신칭) 및 미기록종인 고수씨살이좀벌(신칭)과 긴고수씨살이좀벌(신칭)을 처음으로 보 고한다. 본 논문에서는 한국산 고수씨살이좀벌속(신칭)의 검색표와 두 미기록종의 재기재문 및 사진을 제공한다.

본 연구는 관상적 가치가 뛰어난 한반도 자생식물 너도개미 자리[Minuartia laricina (L.) Mattf.]의 산업화를 위한 대량 번 식 기술을 개발하기 위해 수행되었다. 종자 번식 실험에서는 2023년 8월 16일 형태적으로 완전히 성숙한 종자를 채종하여 사용하였으며, 종자의 내부 형태를 관찰한 결과 배가 완전히 발달하여 미숙배로 인한 형태적 휴면(MD)은 없음을 확인하였 다. 또한, 종자를 증류수에 침지시켜 수분 흡수율을 조사한 결과 수분흡수 24시간 후 약 66%의 수분 흡수율을 보여 불투수성 종피에 의한 물리적 휴면(PY)도 없음을 확인하였다. 실온 (22±2℃)에서 후숙처리(Dry after-ripening)를 0, 4, 21주 실 시 후 각각 4, 10, 15, 20, 25, 30℃로 설정된 기내 발아 실험을 진행하였다. 실험의 결과, 후숙 4주 처리 후 20℃에 치상한 종자 의 발아율이 약 76%로 가장 높았다. 후숙 처리를 하지 않은 종자는 12주 내 어떠한 온도 조건에서도 발아하지 않아 생리 적 휴면(PD)으로 판단되었다. 삽목 번식 실험에서는 줄기삽목 (Stem-cutting) 방식으로 진행하였고, 루톤 분제 처리가 발근 에 미치는 영향을 조사하였다. 루톤 분제를 처리하지 않은 처리 구는 발근율과 생존율이 100%로 나타났으며, 루톤 분제가 처리 된 삽수는 이들보다 발근율과 생존율이 통계적으로 유의하게 낮았다. 따라서 너도개미자리 종자는 생리적 휴면 종자로 분류 되며, 4주간의 후숙 처리가 휴면 타파에 효과적인 것으로 확인 되었다. 또한, 너도개미자리 삽목 번식 시 별도의 발근 촉진제 처리가 필요하지 않음을 확인하였다.

목적 : 본 연구는 입체시 검사법들의 재현성과 교환가능성에 대해 알아보고자 하였다. 방법 : 안과적 질환과 관련된 진단을 받은 적이 없으며 교정시력이 20/20 이상인 30명을 대상으로 하였다. 굴 절이상을 확인한 후 입체시 검사(티트무스, 랑 Ⅱ, 티엔오, 프리즈비 검사)를 각각 하루 이상의 간격을 두고 두 번 검사하였다. 검사 간의 신뢰도 분석은 급내상관계수를 이용하여 분석하였다. 결과 : 반복 검사를 통한 입체시 검사의 신뢰도 확인에서 프리즈비 검사(p=0.103, ICC=0.99) 에서만 높은 재 현성을 보였다. 입체시 검사 간의 비교에서는 프리즈비와 티엔오 검사(p=0.358, ICC=0.89)에서 좋은 호환성을 확 인할 수 있었다. 20초미만의 작은 차이를 나타내는 경우 또한 42%로 모든 검사법들의 비교 중 가장 큰 비중을 차 지하는 것으로 나타났다. 결론 : 입체시 검사의 진행은 방문 간에 일정하게 유지되는 것이 좋으나 검사 방법들의 특성에 따라 신뢰도와 호환성이 좋은 입체시 검사와 함께 평가되는 것이 필요하다. 본 연구의 결과 프리즈비 검사가 신뢰도와 호환성에서 좋은 것으로 나타나 다른 검사와 함께 평가하는 것이 유용할 것으로 사료된다.

호접란은 세계적으로 분화뿐만 아니라 절화로도 판매되는 주 요 화훼작물이다. 상업적 종묘 대량생산은 조직배양 기술에 의 해 이루어지고 있으나 우리나라는 아직까지 균일하고 우수한 발근묘 생산 기술이 확립되지 않았다. 생육이 고르고 우수한 발근 배양묘를 생산하기 위한 적정 배양 신초수를 찾기 위해 용적 500mL 유리 배양병에 호접란 2품종(‘Lovely Angel’과 ‘UniVivace’)의 신초를 1, 4, 7, 10개씩 배양한 후 3개월에 지상부 및 지하부 초기 생육 특성을 조사한 결과, 7개의 신초를 배양하였을 때 신초의 생육이나 뿌리 유도 및 생육에 효과적이 었다. 또한, 배양병 재질이 발근묘 생육에 미치는 영향을 확인하 고자 용적이 500mL로 동일한 유리 배양병과 플라스틱 배양병 에 신초 7개 배양 3개월 후 2품종의 생육 특성을 비교한 결과, 2품종 모두 플라스틱 배양병 보다는 유리 배양병이 유의적으로 생육에 효과적인 것으로 확인되었다. 기내 발근묘 생산을 위한 배양병 재질의 영향은 이후까지 지속적으로 영향을 미치는 것으 로 확인되었다. 결론적으로, 균일한 호접란 발근묘 생산은 플라 스틱 재질보다는 유리병 재질의 배양병이 적합하며, 특히, 용적 이 500mL인 유리 배양병의 경우 신초를 7개(묘당 재식면적 5.4㎠) 이내로 배양하는 것이 바람직한 것으로 확인되었다.

As the number of enlistees decreases due to social changes like declining birth rates, it is necessary to conduct research on the appropriate recalculation of the force that considers the future defense sufficiency and sustainability of the Army. However, existing research has primarily focused on qualitative studies based on comprehensive evaluations and expert opinions, lacking consideration of sustained support activities. Due to these limitations, there is a high possibility of differing opinions depending on perspectives and changes over time. In this study, we propose a quantitative method to calculate the proper personnel by applying system dynamics. For this purpose, we consider a standing army that can ensure the sufficiency of defense between battles over time as an adequate force and use battle damage calculated by wargame simulation as input data. The output data is the number of troops required to support activities, taking into account maintenance time, complexity, and difficulty. This study is the first quantitative attempt to calculate the appropriate standing army to keep the defense sufficiency of the ROK Army in 2040, and it is expected to serve as a cornerstone for adding logical and rational diversity to the qualitative force calculation studies that have been conducted so far.

This study aims to examine the expressive characteristics of the New Chinese style in the collection of Chinese fashion designer Ziggy Chen, analyze the inner meaning, and suggest a new direction in Chinese fashion design. As a research method, the background and concept of New-Chinese style occurrence were examined through previous studies, and the characteristics of the New Chinese style expression in fashion were investigated. The characteristics of the New Chinese style were summarized as cultural tradition, historical ethnicity, and pluralistic convergence. Based on these contents, the characteristics of the New Chinese style expression in Ziggy Chen’s men’s fashion design were analyzed. The data collection range was selected as the range of 20 seasons collected from 2012 S/S to 2022 A/W, which was collected on fashion sites and the brand’s official Instagram. The analysis results are as follows. First, subcultural resistance is a retro and ragged decadence formed by combining punk elements and industrial styles based on the clothing styles of lower-class Chinese people in the 17th and 90s. Second, cultural traditionality was influenced by traditional culture by mainland Chinese designers, who expressed the conservative presence of tradition and the Chinese style by looking at it from an oriental perspective. Third, historical ethnicity forms a Chinese fashion culture in which the national spirit and the development of the times coexist, while traditional culture and contemporary social values develop in harmony. Fourth, traditional fashion develops by combining a contemporary aesthetic sense and lifestyle with pluralistic convergence.

얕은 물에서 선박과 바닥의 상호작용으로 인해, 제한이 없는 깊은 물에서 운항할 때와 비교하여 저항이 증가하는 현상이 발생 한다. 이러한 천수효과에 의해 증가하는 저항은 주로 조파저항에 기인하기 때문에, 본 연구에서는 유람선을 대상으로 LCG(Longitudinal Center of Gravity)의 위치 변경을 통해 성능을 최적화하여 조파저항을 감소시키는 것을 목표로 진행하였다. 수치해석 시뮬레이션을 통해 LCG 위치를 최적화하여 저항의 최소값을 찾고, 이후 수심의 깊이에 따른 영향을 분석하였다. 분석 결과, 37.5% - 52.5% Lpp의 영역에서의 LCG 변화는 총 저항에 큰 영향을 주었으며, 깊은 물의 조건에서는 총 저항의 최대값과 최소값을 비교하였을 때, 72.67%의 큰 차이를 보이 는 반면, 얕은 물 조건에서는 그 차이가 62.97% 정도로 비교적 낮은 차이를 보인다. 수심의 깊이에 따른 효과는 수심이 낮을수록 총 저항 이 증가하는 경향을 보였다. 깊은 물과 비교하여 1.5m의 얕은 물에서는 총 저항이 최대 67.68% 가량 증가하는 것으로 분석되었다. 이 경우 총 저항 증가의 주요 원인은 전체 저항의 84.99%를 차지하는 조파저항에 의한 것으로 판단된다.

Background: Balance is the foundation of performing daily activities, and has been proven to be improved by various compression materials. As a new and never-before-seen means, the floss band improves joint range of motion, increases muscle flexibility, and affects balance. Several studies using the short-term application of a floss band to the ankle have been conducted. However, long-term effects of the floss band on the knee warrant further research. Objectives: This study aims to examine the long-term benefits of strength exercises with a floss band applied to the knee for static and dynamic balance. Design: Quasi-experimental design. Methods: A total of 28 participants (four men and 24 women, aged 20–60 years) with no orthopedic knee conditions were recruited and randomized into two groups, with 14 in the group using the strength exercises with floss bands applied to the knee (the floss band group), and 14 in the group using internal rotation of the tibia during mobilization with movements (MWM; the MWM group). A physical therapist with 10 years of clinical experience applied the intervention 10 times, measuring static and dynamic balance before and after the intervention using the Balance Trainer 4. Independent t-tests and paired t-tests were used for statistical analysis, with a significance level of ⍺=.05. Results: Statistically significant effects for static balance and dynamic balance were observed in the comparison from pre- to post-intervention between the floss band and MWM groups (P<.05). Additionally, a statistically significant effect for dynamic balance was noted in the pre- to post-intervention comparison in the floss band group (P<.05). Conclusion: The strength exercises with floss bands applied to the knee are expected to have a long-term effect on improving dynamic balance.

The electrochemical properties of a CFX cathode were improved by defluorination of the surface with a N2 plasma and using a silica wafer. Compared to the N2 plasma treatment alone, when the CFX and silica were reacted together, the C-F bonds were modified and the surface was etched efficiently, so defluorination was enhanced. An electrochemical analysis confirmed that Half-cells prepared by treating CFx and silica with nitrogen plasma exhibited a capacity of about 400 mAh/g at 5C. In addition, it was confirmed that the loss of charge transfer was reduced by up to 71% compared to that for pristine CFX. As shown by a GITT analysis, when the CFx and silica were treated with N2 plasma together, the ion conductivity gradually increased due to a decrease in the ion diffusion barriers and the formation of a carbon layer. Therefore, this is a simple and effective way to improve the conductivities of CFX cathode materials with the energy of a N2 plasma and the silica-fluorine reaction.

The growing significance of sustainable energy technologies underscores the need for safe and efficient management of spent nuclear fuels (SNFs), particularly via deep geological disposal (DGD). DGD involves the long-term isolation of SNFs from the biosphere to ensure public safety and environmental protection, necessitating materials with high corrosion resistance for DGD canisters. This study investigated the feasibility of a Cu–Ni film, fabricated via additive manufacturing (AM), as a corrosion-resistant layer for DGD canister applications. A wire-fed AM technique was used to deposit a millimeter-scale Cu–Ni film onto a carbon steel (CS) substrate. Electrochemical analyses were conducted using aerated groundwater from the KAERI underground research tunnel (KURT) as an electrolyte with an NaCl additive to characterize the oxic corrosion behavior of the Cu–Ni film. The results demonstrated that the AM-fabricated Cu–Ni film exhibited enhanced corrosion resistance (manifested as lower corrosion current density and formation of a dense passive layer) in an NaCl-supplemented groundwater solution. Extensive investigations are necessary to elucidate microstructural performance, mechanical properties, and corrosion resistance in the presence of various corroding agents to simplify the implementation of this technology for DGD canisters.

Severe Fever with Thrombocytopenia Syndrome (SFTS) is a newly emerging tick-borne disease caused by the SFTS virus (SFTSV), which belongs to the phlebovirus in the Bunyaviridae family. SFTSV is enveloped with a tripartite ambisense RNA genome. The L segment encodes the viral RNA-dependent RNA polymerase, the M segment encodes the two glycoproteins, Gc and Gn, and the S segment encodes the nucleoprotein (NP) and the nonstructural protein (NSs). NP participates in ribonucleoprotein (RNP) packaging and commonly detected early after infection, suggesting that the N protein is possible to be used as a target antigen for early diagnosis of SFTSV infection. In this study, we analyzed a highly immunogenic multi-epitope using GnGc and NP genes from a consensus sequence of SFTSV strain isolated from infected patients in Korea. The selected genes are constructed to the expression vector plasmid pJHL65 and the recombinant plasmid vector was transformed into the Δasd Δlon ΔcpxR Salmonella Typhimurium attenuated strain JOL912 and the expression of these antigens was verified by immunoblotting assay. We observed the significant levels of systemic IgG and mucosal IgM responses against the JOL912-derived antigen in the immunized BALB/c mice. The level of CD3+CD4+, CD3+CD8+ T lymphocyte subpopulation and TNF-α were also highly regulated in splenic T cells re-stimulated in vitro with NP and Gn/Gc multi-epitope selected antigens. Therefore, immunized mice with NP and Gn/GC multi-epitope recombinant proteins of attenuated Salmonella delivery system elicited T cell-related immune response, inducing an effective immune response. In conclusion, the attenuated Salmonella expressing NP-GnGc multi-epitopes could be a novel vaccine candidate against the SFTS virus.

With the advent of the 4th Industrial Revolution, changes in the market environment and employment environment are accelerating due to smart technological innovation, and securing professional manpower and developing human resources for domestic small and medium-sized enterprises is becoming very important. Recently, most of the domestic small and medium-sized enterprises are experiencing hiring difficulties, and the development and training of human resources to overcome this is still lacking in systemization, despite much support from the government. This reflects the reality that it is not easy to invest training costs and time to adapt new employees to small and medium-sized businesses. Based on these problems, the work-study parallel project was introduced to cultivate practical talent in small and medium-sized businesses. Work-study parallel training is carried out in the form of mentoring between corporate field teachers and learning workers in actual workplaces, and even if the training is the same, there are differences depending on the learner's attitude, learning motivation, and training achievement. Ego state is a theory that can identify personality types and has the advantage of being able to understand and acknowledge oneself and others and intentionally improve positive factors to induce optimized interpersonal relationships. Accordingly, the purpose of this study is to analyze the attitudes of learning workers, who are the actual subjects for improving the performance of work-study parallel projects and establishing a stable settlement within the company, based on their ego status. Through this study, we aim to understand the impact of the personality type of learning workers on training performance and to suggest ways to improve training performance through work-study parallelism.

Salinity stress is a major threat to plant growth and development, affecting crop yield and quality. This study investigated the effects of different salinity levels on photosynthetic responses and bulb growth of Lilium LA hybrid “‘Serrada’.” Plants were irrigated with 1 L of 0, 200, and 400 mM NaCl solutions every two weeks for 14 weeks in a greenhouse. At the end of the cultivation period, the substrate pH decreased, and electrical conductivity increased with increasing salinity. Regardless of salinity levels, the days to flowering and number of flowers were similar among treatments. In contrast, the flower width, plant height, number of leaves, and leaf area decreased with increasing NaCl concentrations. Although there were no differences in the photosystem II (PSII) operating efficiency and maximum quantum yield of PSII, net CO2 assimilation rates (An) and stomatal conductance (gs) were significantly reduced at 200 and 400 mM NaCl solutions compared to the control. At 400 mM NaCl solution, bulb diameter and weight significantly decreased at the end of the experiment. These results suggest that bulb growth inhibition could be attributed to limiting photosynthetic rate and stem growth. This finding suggests that salinity mitigation is necessary to maintain plant growth and photosynthetic capacity in lily cultivation on salt-affected soils.

Caprine cryptosporidiosis mainly occurs in young goats, with morbidity rates of 80%–100% and mortality over 50% in goat kids. However, limited research has been conducted on the impact of Cryptosporidium parvum, a diarrhea-causing pathogen, on the intestinal microbiota of goat kids. In this study, 16S rRNA-based metataxonomic analysis was performed to compare the microbial diversity and abundance of the gut microbiota between C. parvum-infected and uninfected goat kids. In total, 12 goat fecal samples were collected, including seven naturally C. parvum-infected and five uninfected goats from Chungcheongbuk-do, Korea. After amplification of the V3–V4 hypervariable region of the bacterial 16S rRNA, high-throughput sequencing was performed. The results showed differences in the microbial composition between C. parvum-infected and uninfected groups based on beta diversity. Firmicutes and Bacteroidetes were the most dominant phyla in both groups. However, no significant difference was observed in the Bacteroidetes/Firmicutes ratio between the two groups. Compared with the uninfected group, the C. parvum-infected group showed significantly higher abundances of Tyzzerella nexillis, Lactobacillus johnsonii, Butyricicoccus pullicaecorum, Enterococcus raffinosus, Enterococcus faecalis, and Negativicoccus massiliensis, and significantly reduced abundances of Aerococcus vaginalis, Faecalicoccus pleomorphus, Oribacterium parvum, and Coprococcus comes. These findings indicate that C. parvum infection, which is associated with diarrhea in neonatal goats, induces alterations in the caprine gut microbiota.

Fluorescent bacteria were isolated from sporocarps that browned into various mushrooms during survey at places of the production in Korea. We examined the pathogenicity, biodiversity, and genetic characteristics of the 19 strains identified as Pseudomonas tolaasii by sequence analysis of 16S rRNA and White Line Assay. The results emphasize the importance of rpoB gene system, fatty acid profiles, specific and sensitive PCR assays, and lipopeptide detection for the identification of P. tolaasii. As a result of these various analyses, 17 strains (CHM03~CHM19) were identified as P. tolaasii. The phylogenetic analysis based on the 16S rRNA gene showed that all strains were clustered closest to P. tolaasii lineage, two strains (CHM01, CHM02) were not identified as P. tolaasii and have completely different genetic characteristics as a result of fatty acids profile, specific and sensitive PCR, lipopetide detection, rpoB sequence and REP-PCR analysis. Pathogenicity tests showed 17 strains produce severe brown discolouration symptoms to button mushrooms and watersoaking of sporophore tissue within three days after inoculation. But two strains did not produce discolouration symptoms. Therefore, these two strains will be further investigated for correct species identification by different biological and molecular characteristics.

It was found in this study that fluorinated microporous carbon aerogels with enhanced hydrophobicity could be successfully prepared by direct fluorination to separate water-in-oil emulsions at high flux. The fluorinated carbon aerogel (F-CA) surface treated by the fluorination method had a water contact angle of 151.2° and could immediately absorb oil. In addition, the unique network structure of F-CA and its hydrophobicity allow surfactant-stabilized water-in-oil emulsions to be effectively and simply separated under gravity without requiring external forces such as vacuum or pressurization. The network structure of F-CAs consists of randomly connected spherical particles that form fluorinated permeation channels, which induce high flux during emulsion separation. The F-CA spherical particles have nanosized pores and high hydrophobicity, which repel and trap water droplets to increase the separation purity. Therefore, F-CA exhibited excellent performance, such as high filtrate purity (up to 99.9954%) and flux (up to 11,710 L/m2h). Furthermore, F-CA reusability was demonstrated as it did not lose its hydrophobicity and maintained its performance even after repeated use. This type of aerogel has great potential to be utilized throughout various environmental fields, including oil remediation.