The current status of insect pollinator use was surveyed. Honeybee was larger than mason bee and bumblebee in average use area per farm household at kinds of insect pollinator. Insect pollinator was used at 19 crops. Among them, tomato was the most used crop, which was 44.9%, and in order to apple 26.7%, strawberry 13.1%, red-pepper 7.7% and watermelon 3.7%. In the use of insect pollinators, bumblebee, which was 56.9%, was higher than honeybee (31.3%) and mason bee (11.8%). The most farmers were a positive intention except for 1.5% of farmer in the use of insect pollinator for crop pollination. The best advantage in insect pollinator use was improvement of seed setting percentage, which was 28.3% and the worst problem was low activity at bad weather, which was 25.8%. And also, 97% of farmers have an intention to use continuously insect pollinator.

Recently Transgenesis was achieved in Bombix mori. For stable and effective transgenesis in B.mori, B.mori cytoplasmic actin gene (BmA3) promoter was used to expression of marker gene, the green fluorescent protein(GFP). Green fluorescent protein expression for selection of transformants was visible in all larval, pupal, and adult tissues but, unexpectdly, was not detectable in embryos. So, it spend times and money on rearing of silkworm. Furthermore, the BmA3 promoter is predominantly active in the midgut, which makes it difficult to reliably identify transformants since autofluorescence of many insect foods can mask low-level fluorescence and only allows the detection of strongly expressing individuals with potentially multiple insertions. Therefore, we need more intensely promoter than BmA3 promoter for selected by expression of GFP in embryos and selected by reliable expression of GFP in larvae. We performed dot blot hybridization to develop strong promoter. Nine differentially expressed clones were isolated and we focused one clone of them which has high similarity with heat shock protein 70 gene from D.melanogaster. We named it as bHSP70 (Bombyx mori heat shock protein 70). Expression from the hsp70 promoter was strong and heat shock-dependent. And Drosophila hsp70 promoter appears useful for regulating expression of Exogenous DNA. So, we analyzed transcriptional activity of promoter with bHSP70 gene by using dual luciferase assay system. bHSP70 promoter has about 264 folds more intensely than BmA3 promoter. Also, when bHSP70 promoter treated heat shock(42℃), transcriptional activity incresed 2 times more than normal condition. Therefore, we suggest that bHSP70 promoter is more effective candidate for stable transformation and selection of transformants.

In order to select the superior lines of bumblebee queens(Bombus terrestris) in oviposition and colony development which has some characteristics of diapause with adult type, undiapaused bumblebee queens of 3rd generation were surveyed in room condition compared to queen treated with CO2 and artificial hibernation. Oviposition rate and preovipositional days of undiapaused queens was lower in efficacy with 46.7% and shows same trend in preovipositional days with 52.8 days than the other treatment with CO2 anesthesia and artificial hibernation with 60.0%, 86.7%, 26.1 days and 26.6 days respectively. In continuous experiment, we also surveyed ovipositional characteristic and colony development of three types of queens emergenced from undiapaused queens and treated queens with CO2 and artificial hibernation. Although undiapaused queens in 1st generation were more effective in the same condition than treated queens, but the undiapaused queens of 3rd generation emergenced from undiapaused queens of 2nd generation were very low trend in all checkpoints compared with treated queens in same generation. This results was shown that the queens treated with CO2 or artificial hibernation need to be in successive mass rearing continuously in the year round.

In this study, the transient second or third layer on the topside of the Martian ionosphere were investigated with the most recently released Mars advanced radar for subsurface and ionospheric sounding/Mars Express data obtained from January 2010 to September 2011 to study the correlation between these topside additional layers and surface magnetic fields, solar zenith angle and solar activities. When examining the zones where the topside layer appeared, the occurrence rate of the topside layer was low at the areas with a strong Martian crustal magnetic field as observed by the Mars global surveyor. The occurrence rate of additional layers on the Martian topside ionosphere decreases as the solar zenith angle increases. However, these layers appeared significantly near the terminator of which solar zenith angle is 90°. In comparison between F10.7 which is the index of solar activities and the occurrence rate of the topside layer by date, its occurrence rate was higher in 2011 than in 2010 with less solar activities. The result of this study will contribute to better understanding of the environments in the topside of the ionosphere through the correlation between the various conditions regarding the Martian ionosphere and the transient layer.

“Jokyoung”, a new bread making wheat cultivar, was developed from the cross between “Seri 82”, a hard white wheat from CIMMYT, Mexico and “Keumkang”, a hard white wheat with high milling rate and early maturing from Korea by National Institute of Crop Sci