Inventory inspection is an important task to eliminate the inconsistency between real assets and inventory management systems, and it is performed periodically at the site. In the case of a large-sized property, it is mainly managed in an outdoor workshop or a warehouse. However, due to the large area of the outdoor area, it is relatively difficult to identify the quantity and location of the property and is also vulnerable to theft. To solve this problem, we proposed a method and system for performing a warehouse inventory inspection using an unmanned aerial vehicle (UAV) and Bluetooth low energy (BLE) beacons. The proposed method has the following advantages. First of all, if we carry out inventory inspection by utilizing UAV, we can minimize user’s effort compared to existing methods. The method of recognizing the asset by attaching the BLE beacon is more costly than using the existing RFID technology, but it is advantageous because the recognition distance is increased and the battery life of the tag is drastically increased. We also designed a BLE beacon reader for the system and implemented a prototype to show the feasibility of the proposed system. The prototype is based on Genuino 101, which is a kind of arduino, and adds HM-10 and Neo-6m modules to provide Bluetooth and GPS functions. The BLE beacon reader was tested in outdoor, and attached in drone. We also developed an inventory checking system based on the web to display results of inventory checking. The proposed approach enables the users to automate the operation of inventory checking on an outdoor warehouse.
Sulfated polysaccharides are known to be immune-stimulators in mammals and can be used as food additives to enhance immunity. In this study, the immune-stimulating activity of water-soluble anionic macromolecules F2 fractionation isolated from Codium fragile using ion-exchange chromatography was tested in olive flounder, Paralichythys olivaceus, in vitro and in vivo. The gene expression of interleukin (IL)-1β was adopted to check the immune-affection. As a result, in vitro study revealed that the expression of IL-1β was significantly upregulated in head kidney cells by 1 and 5 μg/ml of polysaccharides 4 h and by 5 μg/ml of polysaccharides at 24 h. In vivo, IL-1β gene expression in head kidney was significantly upregulated by 20 and 100 μg of the polysaccharides at day 1 post-i.p. injection, while downregulated at day 3 but not significant. Meanwhile, in peritoneal cells, it was upregulated by 20 μg of the polysaccharides at day 1 but the upregulation was sustained until day 3 though it was not significant. These results indicate that the sulfated polysaccharides from C. fragile are an immune-stimulator and might be potential feed additives for olive flounder.
Up to now the complete mitochondrial genome (mitogenome) sequences of only three species of clitellate have been available. We have determined the complete mitogenome sequences of the elusive Burmese giant earthworm Tonoscolex birmanicus (Clitellata: Megascolecidae), which is endemic to Myanmar. The 15,170-bp long genome contains the 37 genes typical of metazoan mitogenomes [13 protein-coding genes (PCG), two rRNA genes and 22 tRNA genes] and one major non-coding region. All of the 37 genes are transcribed from the same DNA strand. The arrangement of the T. birmanicus mitogenome is identical to that of two within-ordinal species Lumbricus terrestris and Perionyx excavates. All 13 PCGs start with the ATG. For the stop codon, only six PCGs end with the TAA, whereas the remaining ones ends with the incomplete stop codon, T. Genes overlap in a total of 14 bp in five locations, and harbor a total of 16 bp of intergenic spacer sequences in nine locations.
SERPINB3 (also known Squamous cell carcinoma antigen 1, SCCA1) is involved in apoptosis, immune response, cell migration and invasiveness of cells. It has been investigated in various types of squamous cell carcinoma. Therefore we investigated the functional role of SERPINB3 gene in human epithelial ovarian cancer (EOC) using laying hens, the most relevant animal model. In 136 laying hens, EOC was found in 10 (7.4%). We compared the expression and localization of SERPINB3 using RT-PCR, quantitative RT-PCR, in situ hybridization and immunohistochemistry, and SERPINB3 activation was detected in chicken and human ovarian cancer cell lines using immunofluorescence microscopy. Thereafter, we examined the prognostic value of SERPINB3 expression in patients with EOC by multivariate linear logistic regression and Cox’ proportional hazard analyses. In present study, SERPINB3 mRNA was induced in cancerous ovaries (p< 0.01), and it was only expressed in the glandular epithelium(GE) of cancerous ovaries of laying hens. SERPINB3 protein was localized predominantly to the nucleus of glandular epithelium in cancerous ovaries of laying hens, and it was abundant in the nucleus of both chicken and human ovarian cancer cell lines. In 109 human patients with EOC, 15 (13.8%), 66 (60.6%) and 28 (25.7%) of those patients showed weak, moderate and strong expression of SERPINB3 protein, respectively. Strong expression of SERPINB3 protein was a prognostic factor for platinum resistance (adjusted OR, 5.94; 95% Confidence Limits, 1.21-29.15). Therefore SERPINB3 may play an important role in ovarian carcinogenesis and be a novel biomarker for predicting platinum resistance and a poor prognosis for survival in patients with EOC. This research was funded by the World Class University (WCU) program (R31-10056), Basic Science Research Program (2010- 0013078) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology and by the Next-Generation BioGreen 21 Program (No.PJ008142), Rural Development Administration, Republic of Korea.
The multicolored Asian ladybird beetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), is common to a wide range of natural and agricultural habitats. Applications of gamma irradiation minimized the losses of stored food and the death or failure of emergence in larval and pupal stages. On the other hand, degrade toxin waste as one of alternative to chemical pesticide for both quarantine and sprout control purposes of storage crop pest. So, we have investigated whether gamma irradiation exposed to eggs, lava, pupa and adults of H. axyridis. It may be affected the emergence, fertility, fecundity, development period and sex ratio of H. axyridis. Some changes of physiological characteristics may be applied to more efficient agents as biological control of several aphids. Insects were exposed to gamma irradiation from 0 to 500 Gy of 60Co depended on their developmental stages. The results showed that the first instar, eggs, third instar, pupae, and adults were more sensitive in order of irradiation dose. And fecundity and fertility of female adults were significantly decreased with increasing gamma irradiation dose at all tested individuals.
We present UBV CCD photometry of Be 2, previously unstudied open cluster. Our photometry covers a field of 3'.2×3'.8 of the sky centered on the cluster, which is slightly smaller than the cluster diameter estimated to be about 260". We have determined the reddening, distance, age and metallicity of the cluster by fitting the Pad ova isochrones to the observed stellar distributions in color-magnitude diagram as well as main sequence fitting: E(B-V)=0.8±0.05, (m-M)o=13.6±0.1, log(t) =8.9±0.1, and Z=0.008. The present photometry shows that Be 2 is a distant open cluster of intermediate age. that it is a distant intermediate-age open duster.
We have analyzed vector magnetograms and Hβ filtergrams of two sunspot groups, one in a growing phase and the other in a decaying phase. In this study, the temporal evolution of their magnetic morphology has been investigated in association with solar activity. The morphological variations of the growing and decaying phase of these sunspots revealed in detail the coalescence of small spots into a large spot and the fragmentation of a large spot into many small spots, respectively. Numerous small flares were detected in the spot group during the decaying phase. This seems to be intimately associated with the shearing motions of many spots with different polarities created by fragmentation of a large sunspot. The magnetic flux and the average shear angle are found to be substantially reduced during the decaying phase, especially in the course of the flarings. This implies that the decaying phase of the sunspot is, to some degree, involved with magnetic field cancellation. The growing spot group has not shown any large activities, but numerous small spots have grown into a typical bipolar sunspot.
The evolution of the Galaxy is examined by the halo-disk model, using the time-dependent bimodal IMF and contraints such as cumulative metallicity distribution, differential metallicity distribution and PDMF of main sequence stars. The time scale of the Galactic halo formation is about 3Gyr during which the most of halo stars and metal abundance are formed and ∼ 95 of the initial halo mass falls to the disk. The G-dwarf problem could be explained by the time-dependent bimodal IMF which is suppressed for low mass stars at the early phase (t < 1Gyr) of the disk evolution. However, the importance of this problem is much weakened by the Pagel's differential metallicity distribution which leads to less initial metal enrichment and many long-lived metal-poor stars with Z < 1 / 3 Z ⊙ The observational distribution of abundance ratios of C, N, O elements with respect to [Fe/H] could be reproduced by the halo-disk model, including the contribution of iron product by SNIs of intermediate mass stars. The initial enrichment of elements in the disk could be explained by the halo-disk model, resulting in the slight decrease and then the increase in the slopes of the [N/Fe]- and [C/Fe]-distributions with increasing [Fe/H] in the range of [Fe/H] < -1.