In this study spent mushroom substrate has ingredient raising rice bed soil. spent mushroom substrates are organiccontent is 60.72% were nitrogen - phosphoric acid - potassium is 1.39 - 0.89 - 0.81% of the chemical characteristics determine.Post-harvested mushroom substrates of the stabilization process, the temperature of the 20 days time progress in the pH of therise and fall of temperature down were germination index also 77, as identified, Spent mushroom substrate bed soil for raisingrice Ingredient to take advantage of the 20 days or more stabilization process needed to be investigated. Rice seed germinationcharacteristic is in the common bed soil for raising rice ingredients manufactured control group and the comparison in spentmushroom substrate is 10% or less of a mixed experimental population of the germination rate is 82% was more than averagedays to germination and germination energy, even a statistical significant difference is or control group than good level was ok.Growth initial also spent mushroom substrate is 10% or less of a mixed experimental population of shoot dry matter (top) andgrave less than control group higher as confirmed spent mushroom substrates are bed soil for raising rice ingredients are likely totake advantage of the high, as was the judge.

광조사 유무에 따른 수량성 조사 결과 수량은 점등처리구에서 많았으나 상품수량은 소등처리구에서 많았고, 생육특성은 점등처리구가 소등처리구 보다 갓과 대의 형태 모두 작은 경향이었다. LED광원에 따른 생육특성을 조사한 결과 자실체의 발육기간은 주황색광 및 적색광에서 길었고, 수량은 청색광 및 녹색광에서 많았다. 그리고 유효경수는 청색광에서 많았으나 상품수량은 주황색광과 적색광에서 많았다. 이와 같은 결과 잣버섯의 경우 파장이 짧을수록 갓의 크기가 커지는 경향이었고, 파장이 길수록 대의 길이는 커지는 경향이었다.

팽이버섯 폐배지의 화학적 특성은 질소, 인산, 가리의 총함 량은 6.93%이었으며 유기물함량은 67.78%이었고 pH와 EC는 7.20과 1.46 ds/m이었으며 팽이버섯 폐배지의 안정화 과정 중 에 15일 경과시점에서 온도의 하강과 pH의 상승이 이루어지 었으며 안정화 15일 경과 후에 발아지수의 경우에도 88로 조 사되어 팽이버섯 폐배지를 작물의 생육자재로 활용 시에는 15 일 이상의 안정화과정이 필요한 것으로 조사되었다. 한편 발아특성의 경우에는 오이와 참외의 경우에는 팽이버섯 폐배지의 혼합비율이 20%이하의 경우에는 발아율 등이 대 조구와 비교하여 차이가 없이 양호하였으며 상추와 배추의 경 우에는 10%이내일 경우에는 대조구와 통계적 유의차가 없는 것으로 확인되었으나 파종 20일후 묘소질의 경우에는 오이와 참외 그리고 배추는 팽이버섯 폐배지의 혼합비율이 10% 이 내의 경우에서만 양호한 생육을 보였고 상추의 경우에는 혼 합비율에 상관없이 대조구에 비하여 낮은 생육특성이 나타나 팽이버섯 폐배지의 경우에는 오이와 참외 그리고 배추에서는 10% 이내로 혼합하여 사용할 경우에 활용가능성이 높다고 판단된다.

이번 연구에서는 피마자박, 미강, 수피, 채종박, 돈분슬럿지 총 5종류의 유기물원의 화학적특성과 안정화 과정중의 특성변 화를 조사한 결과 피마자박과 채종박 그리고 미강의 유기물 및 질소등의 합량이 높은수준이었으며 시험재료의 유기물원들 의 안정화과정에 따른 소요일수에서는 피마자박과 채종박 그 리고 돈분슬럿지가 20일이 경과후에 온도가 하강되면서 안정 화되는 경향을 보이고 있으며 이러한 결과는 발아지수에서도 같은 경향으로 나타나 안정화과정 25일째에 피마자박과 돈분 슬럿지가 70이상의 발아지수를 나타내고 있다. 배추와 상추의 초기생육에서는 수피를 제외한 모든 유기물원들이 비슷한 생 육특성을 나타내고 있다. 이러한 결과를 종합하여 볼 때 피마 자박과 돈분슬럿지를 20일이상 안정화과정을 진행하면 작물에 안정적인 유기물 공급원으로 사용할수 있을것으로 판단된다.

Lentinula edodes mushrooms cultivated under different relative humidities were wrapped at 4℃ and the results of storage characteristics were investigated. Changes in water content of fruiting bodies during the storage period showed the highest water content in fruit bodies harvested from the treatment with the highest relative humidity. The luminosity of the fresh fruiting bodies showed no significant change during the storage period, and the redness was higher in the relative humidity 95% treatment than in the other treatments. According to this study, the relative humidity of the pileus was 65%, and the content of Ergosterol was 0.67 ± 15 g / L at relative humidity of 65%, 80% and 95%. In addition, amino acid analysis and Principal Component Analysis (PCA) confirmed that methionine was the main cause of changes in storage time and relative humidity.

This study analyzed the quality characteristics of black garlic made from Seosan Yukjok Garlic and elephant garlic in Seosan, Chungnam province. Of the inorganic components, Mg content was the highest in all treatment groups, and the Ca content was high in each of the 15 day treatments. The content of K was high after 10 days aging in Yukjok garlic and after 15 days in the elephant garlic. The Fe, Na, K, and Mg content was high in Yukjok black garlic after 15 days, and Na, K, Ca, and Mg were high in the elephant black garlic aged for 15 days. The crude fat content was high in both Yukjok black garlic and elephant black garlic after 15 days. Vitamin C content was highest in both types of garlic after aging for 15 days. An analysis of four kinds of organic acids showed that citric acid was the only organic acid to appear in raw garlic of Yukjok garlic and elephant garlic. Black Yukjok garlic and elephant black garlic had a greater total amino acid content than the raw garlic of either type. However, among the tested amino acids, 13 kinds of amino acids were at their highest after five days of ripening in Yukjok black garlic, while 15 kinds of amino acids were abundant in elephant garlic after the same period. Eight kinds of amino acids were high after aging for 15 days. Through this study, it was confirmed that, in the process of making black garlic, changes in the main components of the garlic occur through different routes, and these changes vary depending on the garlic species. Therefore, this study provided basic data for the processing of Seosan's Yukjok black garlic and elephant black garlic.

The purpose of this study was to investigate eco-friendly measures to manage major diseases which cause heavy economic damages to ginseng. Morphological, physicochemical, and molecular biological species identification was carried out after isolating useful antagonistic bacteria from ginseng fields. In addition, optimal conditions for mass culture were established, and he efficacy of the bacteria in the prevention of the diseases was verified in the field. The results showed that about 150 bacteria were extracted from 150 ginseng fields in the whole county. Among them, B. pyrrocinia LA101 was finally selected, which had a strong antagonistic potency against Alternaria panax, Botrytis cinerea, Rhizoctonia solani, and Cylindrocarpon destructans on agar media. The B. pyrrocinia LA101 is a baculiform gram-negative bacterium identified as Burkholderia pyrrocinia according to results from an API(Analytical Profile Index) kit, 16S rRNA, and gyrase gene sequencing analysis. It was donated to the microbe bank of the Agricultural Genetic Resources Center at the National Academy of Agriculture Science under the Rural Development Administration on September 28, 2011 (Donation No. KACC91663P). A patent for the mass culture technology was granted in August 2012 (Patent No. 10-1175532).

The effects of amino acid and/or urea liquid fertilizer application on the growth and phytochemicals of Perilla leaves were summarized as follows; The fresh weight of the perilla leaves was in the order of CF, CL, KLF, and control, and 39.7 g, 37.4 g, 36.5 g and 32.3 g were measured. The plant height increased by 71.6 cm in the CF treatment than in the control(54.6 cm). The number of nodes was 14.3 node in CF treatment and 19% more than control(12 node). The vitamin C content tends to be increased by fertilizing the amino acid solution in the perilla leaf. The components of polyvalent unsaturation of n-6 origin were measured in CF treatment, KLF treatment, and control in 10.19 mg, 10.18 mg, and 9.38 mg per 100 g, respectively. Glutamic acid, aspartic acid, leucine, arginine, alanine and lysin were contained in perilla leaf amino acid. Glutaminic acid content was found to be 455.1 mg, 495.6 mg, and 478.8 mg in the control, KLF and CF treatment per 100 g, respectively. Effective nutrition management using amino acid fertilizer optimizes crop yield and profitability, it is important to reduce the negative environmental risks of using fertilizer.

This study was carried out to investigate the changes in the amount of 20 free amino acids in PDB (Potato Dextrose Broth) medium during the incubation period while cultivating Lentinula edodes. The total incubation period was 90 days, and the total amount of free amino acids was confirmed every 45 days. Among the 18 kinds of amino acids whose increase and decrease patterns were confirmed, 10 amino acids were increased compared to that in the control, among which cysteine increased from its initial value of 9,889 ± 3 μg/L to 12,909 ± 2 μg/L at 45 days and 29,256 ± 4 μg/L at 90 days. Six amino acids with decreased expression patterns were identified. Arginine decreased to 83,751 ± 2 μg/L after 45 days from its initial value of 161,787 ± 1 μg/L and to 79,055 ± 7 μg/L at 90 days.

This study investigated the relationship among seven species of trichoderma through the identification of strains collected in Korea. The phylogenetic tree among the collected strains was classified into four groups. The trichoderma strains isolated in this way showed inhibitory effect on the fusarium wilt which is parasitic to cotyledon stem..The invisibility of J9, J10, J13 and J16 strains were higher in comparison with other strains in vitro test stand, and their spore production level was also higher. In the aluminum ring tests, it showed that the yield of the spores in J9, J10 and J13 were more than any other strain. As a result conducting the port test for cucumbers, the plant lengths of J13 were larger than the control plot, and the root lengths of all strains, except for J2 were longer than the control plot, and the root weights of J1, J9, J10, J13 and J16 were larger than the control plot. The disease severity for the fusarium wilt showed the smallest values at J13 and J16 in comparison with the control plot, and the control values of J13 and J16 were higher than other strains.

As for chemical characteristics of cultivated media waste, the total content of nitrogen, phosphoric acid, and potassium was 6.93%; organic matter content was 67.78%; pH and EC were 7.20 and 1.46 ds/m, respectively. Organic acids identified during stabilization included citric acid, oxalic acid, fumaric acid, and acetic acid, which was highest. During the stabilization of cultivated media waste, temperature decreased and pH increased on the 15th day, and the germination index was 88 after the period, indicating that at least 15 days of stabilization is needed when cultivated media waste is used for growth material of crop. As for germination characteristics, when the mixture rate of cultivated media waste was 20% and under, germination of cucumber and oriental melon was favorable without difference compared to the control plots. For lettuce and Chinese cabbage, no statistically significant difference was found when the rate was 10% and under compared to the control plots. As for seedling quality after 20 days of sowing, cucumber, oriental melon, and Chinese cabbage showed favorable growth only when the mixture rate of cultivated media waste was 10% and under; lettuce showed lower growth characteristics regardless of mixture rate when compared to the control plots. Cultivated media waste may be significantly useful for cucumber and oriental melon, but not for Chinese cabbage and lettuce.