The Yeongsan River is a prominent inland waterway, alongside the Han River, Nakdong River, and Geum River in South Korea. Numerous bacterial strains were isolated from the Yeongsan River basin for a comprehensive investigation into indigenous prokaryotic species conducted between 2020 and 2023. These bacterial strains were identified using 16S rRNA gene sequencing, wherein 45 bacterial strains shared >98.7% sequence similarities with bacterial species not recorded in Korea thus far. Therefore, this study aimed to catalogue aforementioned unrecorded species and characterize them contingent upon their Gram nature, colony and cell morphologies, biochemical properties, and phylogenetic positions. These bacterial species were determined to be phylogenetically diverse. They were categorized into nine classes, 18 orders, and 25 families. These previously unrecorded species were classified into the following genera and classes: Chitinophaga (class Chitinophagia); Flavobacterium (class Flavobacteriia); Rhodopseudomonas, Gemmobacter, Paracoccus, Azospirillum, Sphingomonas, Novosphingobium, Sphingorhabdus, and Erythrobacter (class Alphaproteobacteria); Bordetella, Pararobbsia, Polynucleobacter, Rhodoferax, Aquabacterium, Malikia, Comamonas, Ideonella, Paucibacter, Undibacterium, Cupriavidus, and Thauera (class Betaproteobacteria); Pectobacterium, Arenimonas, Lysobacter, and Luteimonas (class Gammaproteobacteria); Luteolibacter (class Verrucomicrobiia); Mycolicibacterium, Angustibacter, Ornithinibacter, Janibacter, Schumannella, Aurantimicrobium, Luedemannella, Nocardioides, and Propionicimonas (class Actinomycetes); Geothrix (class Holophagae); and Lactococcus (class Bacilli).

국립원예특작과학원에서는 밝은 화색과 안정적인 화형의 생 육이 우수한 빨간색 스탠다드 장미 품종을 육성하기 위해 진한 적색 스탠다드 장미 품종 ‘엔드리스러브(Endless Love)’를 모 본으로, 꽃잎수가 많고 안정적으로 가시가 적은 밝은 노란색 ‘페니레인(Penny Lane)’ 품종을 부본으로 인공교배하였다. 37 개의 교배실생을 양성해 1, 2, 3차에 걸친 특성검정 및 현장실증 을 통해 꽃이 크고 화형이 안정적이며, 재배안정성 및 생산성, 절화특성이 우수한 ‘원교 D1-390’을 최종 선발하였다. 2023년 ‘루비레드(Ruby Red)’로 명명하여 국립종자원에 품종보호출원·등록되었다. ‘루비레드’ 품종은 밝은 적색(R53C)을 가졌으 며, 꽃잎수가 32.8매, 화폭과 화고는 각각 10.9, 5.9cm로 대조 품종보다 크다. 절화장은 평균 71.7cm, 절화수명은 약 16.7일, 수량은 연간 168대/m2로 대조품종인 ‘레드스퀘어(Red Square)’ 대비 절화장이 길고 절화수명도 2배 이상 길며, 수확량도 1.4배 우수하다. 2023년 국내 육성 장미 품종 서울식물원 관람객 대상 공동평가회에서 스탠다드 장미 중 우수한 평가를 받았으며, 현 장 실증 결과 농가별로 균일하고 우수한 수량과 절화품질을 보 였다. 절화용 장미 ‘루비레드’ 품종은 밝은 적색과 우수한 화형 을 가지는 품종으로 해외 대체 품종으로 국내에서 많이 재배될 것으로 기대된다.

Climate change has led to increased insect pests and pest distribution changes. Traditionally, chemical control using synthetic pesticides has been the main method for pest management, but the emergence of pesticide-resistant pests has become a problem. There is a need to develop new pest control agents to overcome these issues. Entomopathogenic fungi used in pest management have minimal environmental side effects and possess a mechanism of action distinct from that of synthetic pesticides. However, there is a need for the development of technologies to maximize the insecticidal effects of fungi against pests, and expressing and releasing dsRNA within the fungi can preemptively knock out the activation of the insect’s defense system, thereby enhancing the insecticidal effect. Controlling insect defense genes and using entomopathogenic fungi as bio-carriers forms a new pest management strategy. This approach, described as a “microbial insecticide agents development strategy of cassette concept, ” can versatilely modify genes and microbes. It is expected to overcome the limitations of synthetic pesticides.

Silage inoculants, crucial in modern silage production, comprise beneficial microorganisms, primarily lactic acid bacteria (LAB), strategically applied to forage material during ensiling. This study aimed to compare the effectiveness of various inoculants produced by different companies. Five treatments were evaluated, including a control group: T1 (Lactobacillus plantarum), T2 (Lactobacillus plantarum + Pediococcus pentosaceus), T3 (Lactobacillus plantarum + Pediococcus pentosaceus + Lactobacillus buchneri), T4 (Lactobacillus plantarum + Lactobacillus acidophilus + Lactobacillus bulgaricus), and T5 (Lactobacillus plantarum + Pediococcus pentosaceus + Enterococcus faecium). Italian ryegrass was harvested at the heading stage and treated with these silage inoculants. Samples were collected over a 60-day ensiling period. Co-inoculation with L. plantarum and P. pentosaceus (T2) resulted in significantly higher CP compared to the control group co-inoculation exhibited with resulted in Lactobacillus plantarum and Pediococcus pentosaceus in the T2 treatment exhibited higher CP content of 106.35 g/kg dry matter (DM). The T3 treatment, which included heterofermentative bacterial strains such as Lactobacillus buchneri, exhibited an increase in acetic acid concentration (11.15 g/kg DM). In the T4 treatment group, which utilized a mixed culture of Lactobacillus acidophilus and Lactobacillus bulgaricus, the NH3-N/TN content was observed to be the lowest (20.52 g/kg DM). The T5 containing Enterococcus faecium had the highest RFV (123) after 60 days. Expanding upon these findings, the study underscores not only the beneficial effects of particular inoculant treatments on silage quality but also underscores the potential of customized inoculation strategies in maximizing nutrient retention and overall silage preservation.

전 세계적으로 급격하게 성장하는 스마트농업의 중요 요소 중 하나인 자동 관개시스템은 토양수분 센서에서 계측한 데이터를 기반으로 관개시점과 관개량을 판단하기 때문에 토양수분 센서의 설치가 필수적이다. 하지만 국내의 경우 토양을 고려하지 않고 단순히 포장의 가운데에 센서를 설치하는 등 토양수분 센서의 설치 위치에 대한 기준이 마련되어 있지 않아 토양수분 계측 위치에 관한 기준 검토가 필요하다. 본 연구에서 통계학적 방법을 이용하여 토양수분의 대표 계측지점을 선정 연구를 수행하였다. 토양은 수직적 또는 수평적으로 불균일성을 갖기에 구명이 쉽지 않다. 따라서 포장 전체에 걸쳐 지속해서 편향이 발생하지만 특정 위치에서의 평균 토양수분이 시간에 따라 유지한다는 시간 안정성 개념을 기반으로, 평균 토양수분을 나타내는 대표지점 선정 연구를 수행하였다. 토양수분을 측정하기 위한 시스템을 제작하였고, TDR (Time Domain Reflectometry) 센서를 이용하여 총 30개 지점을 측정하였다. 2023년 5월부터 8월까지 측정한 날짜·지점별 데이터를 이용하여, 지점의 편향을 정량화하여 식별할 수 있는 MRD (Mean Relative Difference, 평균상대차이)와 측정의 정밀도를 나타내는 RD (Relative Difference, 상대차이)의 SDRD (Standard Deviation of Relative Difference, 표준편차)를 산출하고, MRD와 SDRD를 통합한 지표로써 RMSE (Root Mean Square Error, 평균제곱근오차) 를 구하여, 시간 안정성이 가장 높은 지점인 RMSE의 수치가 최소인 지점을 대표지점으로 선정하였다. 토양수분 센서로 측정한 데이터를 사용하여 지점별 RMSE를 산출하고 비교하여, 평균적인 토양수분을 나타내는 대표지점을 선정할 수 있음을 확인하였다.

Since rice is the main food in Korea, there are no regulations on corn milling yet. Corn is known as one of the world's top three food crops along with wheat and rice, and it is known that 3.5 billion people worldwide use corn for food. In addition, corn mills are not developed or sold in Korea, but the use of corn mills is increasing significantly in many countries in Southeast Asia. In the Philippines, as Korea's rice mill import increases, Korea's KAMICO (Korea Agricultural Machinery Industry Cooperative) and domestic company A agreed to develop a corn mill jointly with PHilMech, an organization affiliated with the Philippine Ministry of Agriculture. However, research on corn milling was very insignificant, so the development was carried out based on the technology of Korea's rice mill. Rice milling is performed by peeling off the skin of rice and producing brown or white rice, so it is carried out by removing the skin and cutting the skin. On the other hand, in the corn mill, the skin of the corn is peeled, pulverized and selected to produce main products suitable for edible use. Therefore, in order to develop a corn mill, processes such as peeling, transfer, grinding, sorting, and by-product separation are required, and suitable parts must be developed. In addition, the performance must be gradually improved through experiments in which corn is repeatedly milled. The Philippines produces 7.98 million tons/year of corn, which is about 100 times that of Korea, and is mostly consumed as a staple food. This is about 10% of the total crop production in the Philippines. In addition, the main cultivation complexes of corn are the mountainous regions of Tarlac or Pangasinan, and the produced corn is 72.4% of the so-called yellow corn called Arabel and Sarangani, and the remaining 27.6% are known as white corn. In this study, it was intended to produce grains of 2.5 mm or less suitable for food for yellow corn and to develop a corn mill for 200 kg per hour. Detailed conditions for development are stipulated as more than 55% of the main product recovery rate, more than 31% of the by-product recovery rate, less than 5% of the raw material loss rate, and more than 80% of the embryo dislocation rate. In this study, to achieve this, the overall process of the corn mill was developed, and the optimal conditions for the corn mill were obtained through the development of parts and empirical tests to improve performance. In addition, it was intended to achieve the development goal by evaluating and analyzing the performance of each part so that it did not conflict.

Domestic commercial low- and intermediate-level radioactive waste storage containers are manufactured using 1.2 mm thick cold-rolled steel sheets, and the outer surface is coated with a thin layer of primer of 10~36 μm. However, the outer surface of the primer of the container may be damaged due to physical friction, such as acceleration, resonance, and vibration during transportation. As a result, exposed steel surfaces undergo accelerated corrosion, reducing the overall durability of the container. The integrity of storage containers is directly related to the safety of workers. Therefore, the development of storage containers with enhanced durability is necessary. This paper provides an analysis of mechanical properties related to the durability of WC (tungsten carbide)-based coating materials for developing low- and intermediate-level radioactive waste storage containers. Three different WC-based coating specimens with varied composition ratios were prepared using HVOF (high-velocity oxy-fuel) technique. These different specimens (namely WC-85, WC-73, and WC-66) were uniformly deposited on cold-rolled steel surfaces ensuring a constant thickness of 250 μm. In this work, the mechanical properties of the three different WCbased coaitng materials evaluated from the viewpoints of microstructure, hardness, adheision force between substrate and coating material, and wear resistance. The cross-sectional SEM-EDS (Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) images revealed that elements W (tungsten), C (carbon), Ni (nickel), and Cr (chromium) were uniformly distributed within the each coating layers which was approximately 250 μm thick. The average hardness values of HWC-85 and HWC-73 were found to be 1,091 Hv (Vickers Hardness) and 1,083 Hv, respectively, while the HWC-66 exhibited relatively lower hardness value of 883 Hv. This indicates that a higher WC content results in increased hardness. Adhesion force between and substrates and coating materials exceeded 60 MPa for all specimens, however, there were no significant differences observed based on the tungsten carbide content. Furthermore, a taber-type abrasion tester was used for conducting abrasion resistance tests under specific conditions including an H-18 load weight at 1,000 g with rotational speed set at 60 RPM. The abrasion resistance tests were performed under ambient temperatures (RT: 23±2°C) as well as relative humidity levels (RH: 50±10%). Currently, the ongoing abrasion resistance tests will include some results in this study.

This study focuses on the development of coatings designed for storage containers used in the management of radioactive waste. The primary objective is to enhance the shielding performance of these containers against either gamma or neutron radiation. Shielding against these types of radiation is essential to ensure the safety of personnel and the environment. In this study, tungsten and boron cabide coating specimens were manufactured using the HVOF (High-Velocity Oxy Fuel) technuqe. These coatings act as an additional layer of protection for the storage containers, effectively absorbing and attenuating gamma and neutron radiation. The fabricated tungsten and boron carbide coating specimens were evaluated using two different testing methods. The first experiment evaluates the effectiveness of a radiation shielding coating on cold-rolled steel surfaces, achieved by applying a mixture of WC (Tungsten Carbide) powders. WC-based coating specimens, featuring different ratios, were prepared and preliminarily assessed for their radiation shielding capabilities. In the gamma-ray shielding test, Cs-137 was utilized as the radiation source. The coating thickness remained constant at 250 μm. Based on the test results, the attenuation ratio and shielding rate for each coated specimen were calculated. It was observed that the gammaray shielding rate exhibited relatively higher shielding performance as the WC content increased. This observation aligns with our findings from the gamma-ray shielding test and underscores the potential benefits of increasing the tungsten content in the coating. In the second experiment, a neutron shielding material was created by applying a 100 μm-thick layer of B4C (Boron Carbide) onto 316SS. The thermal neutron (AmBe) shielding test results demonstrated an approximate shielding rate of 27%. The thermal neutron shielding rate was confirmed to exceed 99.9% in the 1.5 cm thick SiC+B4C bulk plate. This indicates a significant reduction in required volume. This study establishes that these coatings enhance the gamma-ray and neutron shielding effectiveness of storage containers designed for managing radioactive waste. In the future, we plan to conduct a comparative evaluation of the radiation shielding properties to optimize the coating conditions and ensure optimal shielding effectiveness.

본 연구는 주변 환경의 차이에 따른 화분매개곤충의 유입 특성을 파악하기 위하여 국립수목원 내 진화속을걷 는정원과 부추속전문전시원에 식재된 울릉산마늘의 화분매개곤충을 조사하였다. 2023년 5월 22일부터 6월 2일 까지 꽃이 70% 이상 개화하였을 때 포충망을 활용하여 8일간 곤충을 채집하였고, 각 전시원 별 식생(피도), 기후 (온도·습도·조도)를 조사하였다. 조사 결과 진화속을걷는정원에서 피도 60% 온도 26.4℃, 습도 31.5%, 조도 40953.6lx, 화분매개곤충 20과 450개체, 부추속전문전시원은 피도 90%, 온도 25.6℃, 습도 31.6%, 조도 6387lx, 화분매개곤충 15과 196개체로 나타났다. 온도와 조도가 상대적으로 높은 진화속을걷는정원이 채집된 곤충의 다양성과 방문 빈도가 높았다. 시간대별 곤충의 방문 빈도를 비교해본 결과 온도와 조도는 개체수가 증가할 때 같이 증가하는 경향을 보였으며, 습도는 반대의 경향을 보였다.

단단한 자구를 가진 적색 비모란선인장 ‘Gangjeok’ 품종 은 ‘Isaek’품종을 모본으로, ‘Suyeon’ 품종을 부본으로 하여 2018년에 교배하여 육성하였다. 교배 후 획득한 종자는 조직 배양실에서 기내파종하여 획득한 유묘를 기내에서 삼각주선 인장에 접목하여 ‘1802001’ 등 20계통을 양성하였다. 2019 년에 기내에서 양성한 20계통을 온실에서 삼각주선인장 대목 에 접목하여 재배하면서 ‘1812005’ 계통을 1차 선발하였다. 2020년부터 2022년까지 3차에 걸쳐서 특성을 검정한 후, 농 산물직무육성품종 심의회에서 최종 선발하여 ‘Gangjeok’으 로 명명하였다.‘Gangjeok’ 품종은 편원형의 적색 구를 가진 다. 혹(tubercle)이 돌출된 형태의 모구는 8.4개의 능(rip)을 가지며, 3.5mm 짧은 회색 가시가 발생한다. 정식 10개월 후 ‘Gangjeok’ 품종의 직경은 46.1mm이며, 자구는 평균 18.3 개 발생한다. 2022년 육성계통 평가회에서 ‘Gangjeok’ 품종 은 높은 기호도 점수 4.0을 받았다.

The PoN (Proof of Nonce) distributed consensus algorithm basically uses a non-competitive consensus method that can guarantee an equal opportunity for all nodes to participate in the block generation process, and this method was expected to resolve the first trilemma of the blockchain, called the decentralization problem. However, the decentralization performance of the PoN distributed consensus algorithm can be greatly affected by the network transaction transmission delay characteristics of the nodes composing the block chain system. In particular, in the consensus process, differences in network node performance may significantly affect the composition of the congress and committee on a first-come, first-served basis. Therefore, in this paper, we presented a problem by analyzing the decentralization performance of the PoN distributed consensus algorithm, and suggested a fairness control algorithm using a learning-based probabilistic acceptance rule to improve it. In addition, we verified the superiority of the proposed algorithm by conducting a numerical experiment, while considering the block chain systems composed of various heterogeneous characteristic systems with different network transmission delay.