The objective of this study was to determine the changes in content of polyphenolics, flavonoids, amino acids, especially γ-aminobutyric acid (GABA), and the antioxidant activities in the methanol extracts of four different pigmented rice seeds (white, black, red, and green color) at different germination stages, dry seeds (DS), imbibed seeds (IS), and germinated seeds for 1 (GS1), 2 (GS2) and 3 days (GS3). Total phenolics content at 1000 mg/kg extract was highest in black rice, ranging from 208.3 to 257.3 mg/kg, and followed by red rice 144.7 to 185.7 mg/kg, brown rice 151.3 to 194.2 mg/kg and green rice 125.7 to 160.7 mg/kg (lowest). Total flavonoid level had same tendency to total phenolics among colored rices with significant differences, showing highest amounts (45.2 to 56.8 mg/kg) in black rice and lowest (18.8 to 23.0 mg/kg) in green rice. Total phenolics and total flavonoids levels in black and brown seeds were highest when soaked, and in red rice when nonsoaked, and in green rice when germinated for 1 day. These results showed that content of physiologically- active substances were differ depending on seed coat color, soaking or germinating degree. Content in total amino acids during germination was ranged from 136.5 to 232.0 mg/kg in green rice (highest), and 98.9 to 176.0 mg/kg in red rice (lowest). Total content of amino acids and GABA level in all seed colors were high in order of GS3 > GS1 > IS > DS. The antioxidant activity of the methanol extracts from all the seed colors concentration-dependently increased. DPPH free radical scavenging activity at 2,000 mg/kg was highest in black rice by 83.0 to 87.7%, and lowest in green rice by 44.3 to 65.7%.

The aim of this study was to evaluate the effects of orvus es paste(OEP) on the sperm characteristics during freezing in boar semen. Semen quality was evaluated the motility, membrane integrity, mitochondria function, acrosome status, viability and abnormality. Boar semen were frozen until 5℃ for 2 hours using cell freezer and making the straws, and then freezing by lowing the straws into styrofoam box on the 8cm above the LN2 and plunged into LN2 for cryopreservation. In different concentration of OEP (0, 0.25, 0.5 and 1.0%) into cryo-extender, sperm motility, membrane integrity, acrosome status, viability and mitochondria function were significantly higher in 0.5% OEP than those of any other groups, but sperm abnormality were highest in 1.0% OEP group among all treatment groups (P<0.05). In the relationships of the evaluation methods for sperm viability, CBB vs membrane integrity, CBB vs HO/PI and CBB vs mitocondria function were positively correlated (0.67~0.92). Among the evaluation methods, CBB vs membrane integrity, CBB vs HO/PI and CBB vs mitocondria function were significantly correlated (P<0.001). These results of this study indicate that supplementation of 0.5% OEP in boar semen cryo-extender can improve the semen quality.