This paper presents an electrochemical immunosensor using a graphene/multi-walled carbon nanotube (MWCNT) composite platform for detecting the cardiovascular marker C-reactive protein (CRP). The immunosensor exhibited a linear detection range of 0.20–100 ng/mL CRP with a low limit of detection reaching 0.081 ng/mL. The composite material provided a 3D porous structure that allowed efficient antibody immobilization and minimized steric hindrance. The sensor showed high specificity, with minimal response to interfering substances. Using differential pulse voltammetry, the immunosensor demonstrated exceptional precision, rapid detection, and a direct correlation between CRP concentration and sensor response current. Overall, this work highlights the potential of the graphene/MWCNT composite platform as a robust tool for early CRP detection and cardiovascular disease risk assessment. The immunosensor provides sensitive and selective CRP quantification that could enable timely clinical intervention for at-risk individuals.

Soybean peptide (SP) exhibited low intestinal absorption at oral administration due to its fragile structure under gastric digestion. Therefore, we have attempted to encapsulate peptide by cross-linkage interaction between positive charged chitosan (CS) or chitosan oligosaccharide (CSO) and negative charged peptide. The CS (or CSO) with SP nanoparticles were prepared by using ultrasonification technique. The objective of this study was to find the optimal processing method by changing concentration, pH, and homogenizing conditions. We measured physicochemical properties such as particle size, zeta-potential, encapsulation efficiency (EE%), release rate (RR) and antioxidant ability of samples. The results showed that the optimal processing method was using 0.5% (w/v) CSO (diluted by pH 3 Acetic acid buffer) mixed with 0.5% (w/v) SP (diluted by pH 6 buffer) by 9:1 ratio. Afterwards, using high-speed mixer at 12,000 rpm for 3 min, and then passed 2 times through an ultrasonicator (50% power, 3 min). In this way for processing, the particle sizes of CSO/SP nanoparticles were approximately 300 nm, zeta-potential were approximately 45 mV. In addition, the EE% and RR of CS/SP nanoparticles was higher than the CSO/SP nanoparticles. The increase in antioxidant ability of SP was attributed to the affected by CS/CSO microcapsules. In conclusion, this research can befoundation for the manufacturing process of CS/SP nanoparticles, and it was expected that the future application of this nanoparticle in food matrix.

With the implementation of the internationalization strategy of traditional Chinese medicine (hereinafter referred to as “TCM”) and the increasing recognition of TCM in the world, the export trade of traditional Chinese medicinal materials has shown an overall positive trend. With the rapid development of the global economy, people's pursuit of wealth has gradually accelerated, and at the same time, they have paid much more attention to pollution-free and health and green environmental protection than before. A new measure to protect the domestic ecological environment has emerged internationally - green trade barriers. Based on the analysis of the general situation of green trade barriers and the status quo of the industry, this paper expounds the performance and reality of the green trade barriers encountered in China’s export of Chinese herbal medicines, and then provides four countermeasures: firstly, spread and promote screening and classification standards of regions and material origins for medicines; secondly, resolutely promote industrial production standards to ensure the quality of traditional Chinese medicinal products; thirdly, accelerate the cultivation of international TCM trade talents to help better predicting and responding to the green trade barriers; and lastly, strengthen the overall management of the traditional Chinese medicine industry.

요즘 중한 양국 문화 영역의 합작이 심화하고 있다. '원소스 멀티테리토리'모식으로 제작한 중한 영화들을 관객의 주목을 받았다. 하지만 <용하형경>과 <극한직업>이 개봉하면서 양극화 관영 평가와 현격 한 흥행 차이가 생겼다. 따라서 본 논문에서는 두 영화를 중심으로 내러티브, 캐릭터로서 현지화 원소의 운용을 대비하고 분석한다. 같은 스토리는 어떻게 다른 나라의 현지화 사회 문화를 표현할 수 있는 것에 대해 살펴보고 더 우수한 중국 영화를 만들기 위해 현지화 원소의 활용 방법을 총결해야 한다.