Anthriscus sylvestris (L.) Hoffm. is a perennial herb found widely distributed in various regions of Korea, Europe, and New Zealand. The root of A. sylvestris have been extensively used in the treatment for antitussive, antipyretic, cough remedy in Oriental medicine, but the physiologically active function of the leaf of A. sylvestris is as yet unknown. In this study, we investigated the anti-cancer activity and the mechanism of cell death of water extracts of leaf of Anthriscus sylvestris (WELAS), on human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that WELAS treatment inhibited cell viability in a concentration- and time-dependent manner. In addition, the treatment of WELAS markedly induced apoptosis in FaDu cells, as determined by the viability assay, DAPI stain and FACS analysis. WELAS also increased the proteolytic cleavage of procaspase-3, -9 and PARP (poly(ADP-ribose) polymerase). In addition, exposure to WELAS decreased the expression of Bcl-2 (an anti-apoptotic factor), but increased the expression of Bax (a pro-apoptotic factor), suggesting that mitochondria-dependent apoptotic pathways are mediated in WELAS-induced apoptosis. Taken together, these results indicate that water extracts of leaf of A. sylvestris inhibits cell growth and induces apoptosis via the mitochondrial-dependent apoptotic pathway in FaDu human hypopharyngeal squamous carcinoma cells. Therefore, we propose that the water extracts of leaf of A. sylvestris is a novel chemotherapeutic drug, having growth inhibitory properties and induction of apoptosis in human oral cancer cells.

Antibiotics have been used to prevent disease, promote growth rate, and improve feed efficiency. However, the use of antibiotics in livestock has been restricted worldwide due to problems such as bacterial resistance. Therefore, probiotics among alternatives to antibiotics have gained attention in the livestock feed industry these days. This study was conducted to investigate the effects of dietary supplementation with probiotic 379D on safety, growth rate, and feed efficiency. In this study, bacterial strain 379D was isolated from soil and identified as a Bacillus sp. according to 16S rRNA sequence analysis. In an in vitro test, in-gel activity assay and antimicrobial susceptibility test were conducted to evaluate 379D. In an in vivo study, 379D was administered at concentrations of 0.1% and 1% to broiler chickens for 28 days. The results of in-gel activity assay and antimicrobial susceptibility test showed that strain 379D had broad spectrum antimicrobial activity. Furthermore, no adverse 379D-related effects were observed in 0.1% and 1% groups. Feed efficiency was higher in the 379D-treated groups than in the control group. In conclusion, 379D is expected to be used as a safe alternative to antibiotics in a feed supplement and will improve feed efficiency in broiler chickens.

메밀 식이섬유의 효율적인 활용방안과 종자발아의 특성 연구를 위하여 메밀(신농 1호)을 10℃에서 7일간 발아시켜, 24시간마다 시료를 채취하여 불용성, 수용성 및 총식이섬유의 함량과 조성의 변화를 관찰한 결과는 다음과 같다 발아 전 총식이섬유 함량은 건량기준으로 24.86%였고, 발아 1일째에 다소 감소하였다가 이후 서서히 증가하여 발아 6, 7일에는 32.22, 36.25%로 급격히 증가하였다. 불용성 식이섬유 함량은 발아 전 22.95%였고, 발아중 변화경향은 총식이섬유와 유사하였으며 발아 7일에는 31.11%를 나타냈다. 수용성 식이섬유 함량은 발아전 1.42%에서 발아 후 서서히 증가하여 발아 7일에는 4.53%였으며, 증가율은 발아 7일째에 219.0%로 급격히 증가하였다. 총식이섬유에 대한 불용성 식이섬유와 수용성 식이섬유의 조성비는 발아 전 16.2:1에서 점차 감소하여 발아 5일째에는 6.01:1이었으며, 6, 7일에는 6.54:1, 6.87:1로 증가하는 경향을 나타내었다. AOAC 법과 Prosky 법에 의한 총식이섬유 함량 차이는 0.20∼093%로써 AOAC법이 다소 높았다. 그러나 두 방법간의 평균치 검정 결과 유의적인 차이는 없었고, 상관관계는 높은 정의 상관관계(r=0.9966, p<0.01)를 보였다. 발아중 식이섬유 구성성분들인 불용성 식이섬유(X_1), 수용성 식이섞유(X_2)와 뿌리신장도(Y)와의 함수관계를 다중회기분석한 결과, 결정계수가 R^2=0.968로 매우 높은 함수관계를 나타내 었다. 회기방정식은 다음과 같다. Y = -12.6681 + 0.5089X_1 + 0.6622X_2( p<0.01)