항균력이 있는 것으로 확인된 대황 및 황련추출물을 오이, 호박, 상치 및 고추에 처리하였을 때 총균수가 감소하였다. 채소의 종류에 따라서 적절한 처리농도는 달랐는 데, 호박, 상치 및 고추는 100 ppm, 오이는 500 ppm 처리가 적절한 것으로 나타났다. 채소를 침지할 때의 시간은 10분 정도가 적절한 것으로 나타났으며, 세척수의 온도를 4로 높일 경우에도 채소류 표면의 총균수 감소 효과는 실온의 세척수와 비슷하였다. 또한 오이와 호박을 대

Korean medicinal herb extracts(KMHE) were applied to the preservation of greenhouse produce in order to prove their effectiveness. KMHE showed remarkable antimicrobial effects against Bacillus cereus, Peudomonas syringae, and Corynebacterium xerosis causing the postharvest decay of greenhouse produce. Among KMHE the extracts of Rheum palmatum L. and Coptis chinensis Franch most obviously inhibited the growth of microorganims causing the Postharvest decay of greenhouse produce, which destroyed to undetectable levels when treated with more than 500ppm of KMHE. The activities of KMHE were stable in the wide spectrum of pH and temperature. Direct visualization of microbial cells by using both transmission electron microscope and scanning electron microscope showed microbial cell membrane the function of which was destroyed by treating with the dilute solutions of KMHE. This change of cellular membrane permeability could be identified in the experiment that O-nitrophenyl--D-galactopyranoside(ONPG), the artificial substrate of -galactosidase, was hydrolyzed in the presence of KMHE, indicating that the membrane was perturbed by KMHE.

It is neceesary to evaluate the location suitability of protected cultivation facilities to guide reasonable protected cultivation. The evaluation system could help plan new protected cultivation facilities in rural areas. In this study, an assessment was made for the facilities located in three different selected regions: suburban, plain, and mountainous. The assessment was performed based on spatial characteristics of protected cultivation facilities such as land category, size of protected cultivation facilities, land shape, stoniness, land consolidation level, soil drainage, land slope, topography, effective soil depth, zoning or not of agricultural development area, irrigation and drainage condition, distance from roads, and so forth. The results showed that there were significant differences in locational characteristics among the three regions.