Soil biodiversity is important for proper functioning of soil ecosystem. Soil microarthropods play roles in cycling of nutrients and decomposition of organic matter. We compared the diversity of oribatid mites in organically and conventionally managed apple orchards. Sampling were done from 10 orchards; 5 from organic and 5 from conventional, in spring season of 2011 and 2012. Species richness and abundance were higher in conventional (25, 4,222/m2) than organic orchard (21, 1,906/m2). Diversity index was higher in conventional than organic orchard. Common dominant species present in orchards were Perglumna duplicata nipponica and Scheloribates latipes. Only observed in apple orchards were Pilogalumna tenuiclava, Protoribates agricola, Hypochthonius luteus, Punctoribates manzanoensis, Punctoribates sphaericus, and Scheloribates corpusculum. In organic orchard, Suctobelbella naginata and Tectocepheus cuspidentatus were only present. Pergalumna altera, Galumna longiporosa, and Protoribates lophotrichus, Galumna sp. were found only in conventionally managed apple orchard. Further study on the functional aspects and association with other soil biotic and abiotic factors are demanding.

Grapholita dimorpha is a new pest of apple, pear and plum in Japan and Korea as well. Although G. dimorpha was reported as a multi-voltine insect with four generations per year, the phenology is not fully known in Korea. We present a model to predict the spring occurrence of G. dimorpha adults based on the relationship between the cumulative proportion of G. dimorpha adult catches and accumulated degree-days. Five different distribution models were applied and the selection was made based on the statistical information criteria (AICC and BIC). Model validation was performed with the field data of the male moth catches in sex pheromone-baited traps of two apple production regions (Chungju and Geochang) of Korea in 2011. Model predictions of the dates for the cumulative 50% male moth catches were within five days variation. Because G. dimorpha has a feeding habit of boring inside the fruit the management practice of G. dimorpha should apply on egg and neonate larval stage. The management strategy of G. dimorpha in spring might be improved by the model.

This research is aiming to develop generic attractants for lepidopteran pests that can be used as a control tool for environmentally friendly management against the lepidopteran pests in orchards. During the first year of this research we carried out various trapping experiments in apple orchards in Korea. Field trapping experiments were carried out at two apple orchards in UaiSung-Gun, GyungBuk Province during May – September 2010, using different rice wines and fruit-based fermenting baits. Overall, when six major moth pest species(Grapholita molesta, Carposina sasakii, Adoxophyes orana, Choristoneura longicellana, Archippus breviplicanus, Phyllonorycter ringoniella) in apple orchards were counted, 445 moths were captured in raw material-baited traps and 1,566 moths in sex pheromone-baited traps. There were no clear differences in the attractiveness to the moths between different kinds of rice wines and fruit baits when the attractiveness of fifteen different rice wines and seven fermenting fruit baits were compared in the apple orchards. One rice wine (‘Chung-ju’ rice wine) and fermenting pineapple showed somewhat higher attractiveness to G. molesta than other raw baits, which will be re-examined in 2011 – 2012 periods.

Bombus terrestris was released for pollination of apple flowers at apple orchards in Uileumgol, Milyang-sity, mid spring, 2008. Numbers of out-going bees and in-coming bees were 38heads at 10 a.m. and 23heads at 4 p.m., respectively. 75% bees per total in-coming bees collected the pollens about 10 a.m. and 63% bees per total in-coming bees collected the pollens around 4 p.m.. The SEM photography(A) showed that 82.7% of a pollen load were collected on apple flowers and 17.3% of a pollen load were collected from another plants and trees flowers. The another SEM photography(B) showed that 89.1% of a pollen load were collected on apple flowers and 10.9% of a pollen load were collected from another plants and trees flowers. Therefore these results show that B. terrestris mainly pollinates on the apple flowers but the bee seems to visit flowers of other plant species from time to time.

Monitoring was conducted to investigate the occurrence of Grapholita dimorpha Komai in Korean apple orchards using sex pheromone traps. G. dimorpa showed four peaks per year: early May, from late June to early July, from late July to mid August, and from late August to September. After adult emergence of the over-wintered G. dimorpha, G. dimorpha catches was decreased significantly and increased again after July. In G. molesta traps, G. molesta and G. dimorpha were trapped by 98.8 and 1.2%, respectively. Conversely in G. dimorpha traps, G. dimorpha and G. molesta were trapped by 99.7 and 0.3%, respectively. The 30.6% of the moths from the damaged apple fruits were G. dimorpha. This is the first report on G. dimorpha in apple orchards in Korea.

Major fruit moths in commercial apple orchards are known as Carposina sasakii and Grapholita molesta. In addition, recently G. molesta is dominant species compared with C. sasakii in apple orcahrds. In case of Japan, the fruit damage occurred a lot unexpectedly in apple orchards of the northern Nagano during the harvesting season in 2004. As the result of identifying after emerging the larvae collected from the damaged fruit, Grapholita dimorpha was found out. The morphology of Grapholita dimorpha are almost similar to that of Grapholita molesta, and the shape of the damaged fruits was too similar to distinguish them. We installed sex pheromone traps of Grapholita dimorpha in apple orchards to check if there were Grapholita dimorpha in Korean apple orchards and to survey seasonal occurrence. As a result of survey by installing sex pheromone traps of G. dimorpha at one apple orchard in Uiseong-Gun and Gunwi-Gun area respectively, we could find high population density of G. dimorpha in sex pheromone traps. The peak of the first generation of G. dimorpha was dated in early May. The first generation occurrence of G. molesta in sex pheromone traps was lower than that of Grapholita molesta. However, it had higher occurrence of sex pheromone traps than G. molesta since it increased after mid and late July. Also, as a result of identifying in G. molesta traps, there were 673 G. molesta and 8 G. dimorpha. As a result of survey of 1,102 G. dimorpha in its sex pheromone traps, there were 1,099 G. dimorphaand 3 G. molesta.

The study investigated occurrence of major insect pests and damage to 16-30 representative apple orchards in major apple production area of 4-8 cities and counties in Gyeongsang Provinces and Jeonbuk province for 17 years from 1992 to 2008. In addition, it examined the hatching time of the overwintered eggs of European red mite (Panonychus ulmi) and apple leaf-curling aphid (Myzus malisuctus) at the Apple Research Station in Gunwi-gun, the Gyeongbuk Province from 1993 to 2009, and conducted a survey on the occurrence rate of oriental fruit moth (Grapholita molesta) and peach fruit moth by means of sex pheromone traps at 2-5 day intervals to look into the changes in the occurrence. As the result of survey on the insect pests of representative apple orchards, the population density of two spotted spider mite (Tetranychus urticae) was higher then that of Panonychus ulmi from 1992 to 1999 while it became lower than Panonychus ulmi from 2000. Although there was difference in spiraea aphid (Aphis citricola) by year, but the occurrence density tended to decline from 1992. Lyonetia prunifoliella had not become a problem in apple orchards before the 1990’s, but it started occurring in 1993-1994 and increased rapidly in the late 1990’s. Later, it has consistently increased as leafminer with Phyllonorycter ringoniella in apple orchards. Tortricid insect pests increased the most highly in the late 1990’s, but it has not been a problem after 2000 and the occurrence and damage by Grapholita molesta was higher than that of Carposina sasakii in apple orchards after 1997. Besides, the damage by stink bugs as well as ambrosia beetles has increased after 2000. In the 2000’s, the initial hatching date of overwintered eggs of Panonychus ulmi and Myzus malisuctus ulmi was 18 and 14 days earlier, the 50% hatching date was 7 and 6 days earlier, and the final hatching date was 1 and three days earlier respectively than that of the 1990’s. In the 2000’s, the first occurrence of Grapholita molesta and Carposina sasakii was earlier by 5 days compared to the 1900’s, and the first occurrence of apple insect pests tended to become earlier compared to the 1990’s.

Woolly apple aphid, Eriosoma lanigerum, overwintered as adult or nymph stage on rootstocks, and crown- and root sucker in the soil. In an unsprayed apple orchard, the number of E. lanigerum colony started to increase from mid-April, showed the 1st peak between late June and early July, thereafter decreased followed by the 2nd peak in late July, and then again peaked in late September as the size in the 1st peak. In this orchard, the number of E. lanigerum colonies per tree did not exceed 3.5 colonies during the peak occurrence period, and was maintained around 2 colonies throughout seasons. In all seasons, parasitism of Aphelinus mali on E. lanigerum was much lower on root colonies than on aerial colonies that located on shoots and tree trunks above the ground. The parasitism of E. lanigerum was high in most orchards examined, showing parasitism of > 70% in maximum in most cases. In the laboratory bioassay for the mortality effects of several insecticides on E. lanigerum, fenitrothion, dichlorphos, machine oil, methidathion, thiacloprid, and imidacloprid showed 97.8, 96.8, 95.4, 91.5, 26.7, and 7.8% morality, respectively. Also, the adult emergence rates from A. mali mummies were 51.2, 72.6, 14.2, 3.5, 72.2, and 85.4% in the treatment of the above insecticides, respectively. Insecticides belong to neonicotinoid, which are newly developed to control aphids, showed low mortality against E. lanigerum. Fenitrothion and dichlorphos were effective on E. lanigerum control and had a low toxic to A. mali. Consequently, the insecticides should be useful in integrated pest management system for E. lanigerum in apple orchards.

Spider mite is the most concerned pest in apple production. This study compared and analyzed the historical changes of two mite pests, Tetranychus urticae koch and Panonychus ulmi (Koch) in 16-30 representative apple orchards in the major apple production area; Gyeongnam, Gyeongbuk, and Jeonbuk province of Korea from 1992 to 2007. Monthly sampling of 100 leaves per orchard provided the basic data of population density of two mite species. Among those orchards, chemical spray history was also analyzed from four orchards, which could be representatives of IPM practitioners. It was found that overall population densities of T. urticae were higher than those of P. ulmi for 16 years. Before 2000, T. urticae was dominant over P. ulmi in most orchards. However since 2000, P. ulmi have occurred more than or as much as T. urticae.. Moreover, although there was large fluctuation of occurrences of two mite species over the years and localities, spider mite pressure appeared to decrease, in general. It seemed to relate the timing of ground cover management with pheromone-based IPM implementation nationwide from late 90s. Panonychus ulmi appeared to rise in April and July in general, fall in August, and go up again in the late season; September-October, while T. urticae appeared to begin to rise in June with July or August peak and sometime with late season second peak in October. Application frequency of acaricide has been dropped from four times in the late 1990s to 2.5 times in the late 2000s.

Six kinds of insecticides, fenpropathrin, dicofol, milbemectin, tebufenpyrad, monocrotophos and propargite, were selected for investigating LC50s to local two spotted spider mite(TSSM) strains. The TSSMs were collected from peach (Cheongdo area) and apple orchards (Chungju, Kunwi and Sobo areas) in 2005, from strawberry plastic house (Nonsan and Damyang areas) in 2006 for experiment and some insecticides selected TSSM strains for more than 20 generations were also used. Each strain was clustered by LC50 value got from six selected insecticides treatment. Every insecticide showed about three-fourfold LC50 difference among TSSMs caught in same area. There are no specific differences in LC50 patterns of TSSM to insecticides within peach orchard and among apple orchards. But TSSMs of apple orchard and peach orchard were clustered into different group each other. Grouping of selected TSSM strains with insecticides in lab artificially showed good relationship between action mechanisms of insecticides. In strawberry strains TSSM showed regional differences between Damyan and Nonsan. The TSSMs of Nonsan showed more resistance to Tebyfenpyrad and Propargite, but TSSM of Damyang was more resistant to fenpropathrin than that of Nonsan. The TSSMs selected with monocrotophos and bifenazate, known as acetylcholinesterase inhibitors, and also pyridaben and chlorfenapyr, known as inhibitors of mitochondrial electron transportation, were grouped close respectively.

This survey was conducted from 1992 to 2005 in the major apple producing districts in southern part of South Korea including 4~8 cities, to know the occurrence and damage level of two major pests, Carposina sasakii and Grapholita molesta that attack apple fruit. The fruit damage by G. molesta during the harvest period ranged from 0.02 to 1.64%. A tendency of higher damage of G. molesta was observed after 1997 compared with the previous years. The other hand, the fruit damage by C. sasakii was 0.02 to 1.30%, and the damage level was very low with fruit damage of ＜ 0.3% except 1998. The orchard infested with G. molesta was 13 to 71%, while 12 to 57% with C. sasakii. The rates of orchards where fruit damage by G. molesta was found were higher than those by C. sasakii after 1997. The tendency of fruit damage rates in the orchard where the most fruit damage was found was same with the trend of orchard rates infested with the pests. The maximum damage rate by G. molesta was 20.0% in 2005, while 4.5% by C. sasakii in 1998. The damaged shoot rates by the first generation G. molesta was 0.1~8.1%, and it had a positive correlation with the rates of fruit damage during the harvest period. Consequently, it is concluded that G. molesta is dominant species compared with C. sasakii in commercial apple orchards recently.

수원시 이목동에 있는 원예연구소의 사과원에서 조사한 1958년부터 1998년까지 자료를 바탕으로 사과응애와 점박이응애의 역사적 발생변동 과정을 추적하였다. 1970년까지는 사과응애가 우점하였고 1980년 이후에는 점박이응애로 우점종이 바뀌었으며, 그 변화는 1970년 중반을 전후로 나타났다. 이러한 변화를 설명하기 위하여 3가지 가정, 즉 첫째, 두 종의 경쟁에서 점박이응애가 승리한다. 둘째, 과원의 초생은 점박이응애의 수상이동 여부를 결정하는 중요한 요소이다, 즉 초생이 파괴되었을 때 점박이응애의 수상이동이 촉진된다. 셋째, 과원생태계가 교란되지 않을 때 응애류는 천적에 의하여 개체군 밀도가 조절된다 하에 약제살포(고독성 비선택적 약제 처리 및 선택적 저독성 약제처리)와 초생관리(초생재배 및 청경재배=제초제 살포)에 따른 두 종의 장기적 상호작용 결과를 추정하였다. 고독성 농약/초생재배 시스템에서는 점박이응애의 수상이동이 제한되어 사과응애는 경쟁을 피할 수 있고, 또한 천적상이 파괴되기 때문에 전 생육기간 동안 사과응애가 우점한다. 고독성 농약/청경재배 시스템에서는 계절초기부터 점박이응애가 수상으로 이동하여 사과응애를 경쟁적으로 배제시킴으로써 점박이응애가 우점한다. 저독성 농약/초생재배 시스템에서는 점박이응애의 수상이동이 제한되어 사과응애가 우점하지만 천적의 작용으로 높은 밀도를 형성하지 못한다. 저독성 농약/청경재배 시스템에서는 점박이응애가 수상으로 이동하여 사과응애와 경쟁하지만 천적의 작용으로 밀도가 낮아져서 경쟁압력이 낮아지므로 생육후기 사과응애의 발생이 가능하다. 따라서 계절초기 사과응애, 계절후기 점박이응애가 우점하는 형태로 공존할 수 있다. 본 자료에서 제시한 점박이응애 우점화 현상은 부분적으로 고독성 농약/청경재배 시스템 상태에서 두 종의 장기적 상호작용의 결과로 설명될 수 있었다. CAD를 이용하여 살펴보고자 한 것이다. 연구방법으로는 가장 일반적인 실로 묶기, 전통적인 손바느질 느낌이 나는 시침질, 현대적 느낌이 강한 깡통에 의한 묶기와 기하학적 효과가 나는 접기 등의 홀치기염색 기법으로 수작업 한 다음 CAD를 이용하였다 연구의 결과는 다음과 같다. 첫째, 홀치기염색기법에 의해 제작된 패턴을 모티브로 하여 수작업에서 얻지 못하는 다색사용가능성이 주�

1998년부터 2000년까지 3년간 경북사과주산지 사과원과 묘목원을 대상으로 잎말이나방류 종을 조사하였으며, 1992년부터 2001년까지 10년간 5-6개 시군의 관행사과원과 폐원에서 잎말이 나방 발생동향과 피해정도를 조사하였다. 사과원에 발생하는 잎말이나방으로는 애모무늬잎말이나방 (Adoxophyes orana), 사과무늬 잎말이나방(Archips breviplicanus), 매실애기 잎말이나방(Rhopobota unipunctana), 사과잎말이나방(Choristoneura longicellana), 복숭아잎말이나방(Acleris fimbriana), 감나무잎말이나방(Ptycholoma lecheana circumclusna), 한국잎말이나방(Archips subrufanus) 등 총 7종이 조사되었다. 이중 애모무늬잎말이나방이 매년 단연 우점종이었으며, 사과무늬잎말이나방과 매실애기잎말이나방은 특정 해에만 1-2 농가에서 다발생하였다. 1980년대 우점종은 사과무늬잎이나방과 매실애기잎말이나방이였으나, 1990년대말 우점종 변화가 이루어진 것으로 조사되었다. 10년간 관행사과원의 잎말이나방 피해정도를 조사한 결과, 평균 피해과율은 였다. 과실 피해는 3세대 유충에 의해 8-10월에 집중적으로 이루어졌다