The aim of this study was to determine total phenolics (TP) content, total flavonoids (TF) level, and antioxidant activity of methanol extracts from leaf parts of 15 Vietnamese wild plants. TP content showed the highest amount in methanol extracts from Altenanthera sessilis (409.7±1.4 ppm), and followed by Eclipta prostrata (183.6±3.8 ppm) and Cassia tora (159.3±5.7 ppm). The highest TF level also was found in A. sessilis (156.3±1.7 ppm), followed by E. prostrata, and C. tora, showing similar tendency to TP. Methanol extracts of A. sessilis, E. prostrate and C. tora at 1000 ppm exhibited the highest DPPH radical scavenging activity by 94.5, 92.6 and 92.7%, respectively. The methanol extracts from A. sessilis showed the highest ABTS and nitrite scavenging activities by 97% and 92%, respectively. The highest correlation coefficient (r2) was 0.9522 between TP and TF, and followed by 0.8919 between DPPH and ABTS activities. However, polyphenols and antioxidant activities showed low correlation coefficients, ranging from r2=0.4114 to 0.4826. It was concluded that Vietnamese wild plants contain polyphenol compounds with antioxidant activities depending on plant species.

본 연구에서는 국내에서 시판되는 코코아 분말로부터 polyphenol 분획물의 다양한 in vitro 항산화활성을 조사하였다. 코코아 80% 메탄올 추출물로부터 분리한 0.01N 염산, 에틸아세테이트 및 산성 메탄올 분획물의 총 페놀화합물 함량은 각각 19.25, 202.24 및 295.83 mg/g이었다. 코코아 용매분획물의 DPPH 라디칼 소거활성을 농도의존적인 경향을 보였으며, ABTS 라디칼 소거활성은 산성 메탄올 분획물에서 가장 높은 활

This study was carried out in order to determine the biological activities such as antioxidant activities in nine cultivars of Momordica charantia L. (Korea, China, Japan and Philippine native cultivars). Their antioxidant activities were measured using DPPH free radical scavenging, ABTS cation radical scavenging, and Nitrite scavenging ability. The highest total polyphenol content (16.82 μg/ml) measured in the native Korea cultivar, and this value was 4.0 μg/ml higher than that of the 'Verde Beuhas' cultivar (12.82 μg/ml). The 'Peacock' cultivar had the highest total flavonoid amount which was 4.38 μg/ml. The free radical scavenging activity using DPPH method was the highest in the native China cultivar (RC50 = 102.6 μg/ml). ABTS cation radical scavenging activity according to cultivars was significantly higher in 'Peacock'. Nitrite scavenging ability showed the most remarkable effect at the pH 1.2, exhibited to 81.5~86.9% by addition of ethanol extract 1μg/ml from Momordica charantia L. These results suggest that Momordica charantia L. had the potent biological activities, and that their activities exhibited differently depending on cultivars.

블랙 라스베리 과실의 성숙정도에 따른 항산화 활성과 이와 관련된 인자인 총페놀성 함량 및 비타민 C 함량의 변화를 측정하였다. 이러한 결과들로부터 과실의 성숙 단계에 따른 활성 및 성분변화 그리고 이들 간의 상관관계를 구명하였다. 블랙 라스베리 과실의 항산화활성측정 결과, 자유 라디칼 소거능 및 환원력 모두 과실이 성숙함에 따라 감소하였다. 또한 항산화 활성과 밀접한 관계가 있는 총페놀 함량 및 비타민 함량 역시 과실이 성숙함에 따라 감소하였다. 자유 라디칼 소거능은 과실 결실 5일 후에서 125ppm 농도에서 61.67%로 최대치를 나타내었으며 환원력은 과실 결실 10일 후가 0.71로 최대였다. 총페놀 함량과 비타민 C함량의 최대치는 각각 220.73ug/g과 540.45ug/g였다. 여러 인자들에 의한 상관관계 분석 결과 자유 라디칼 소거 활성과 환원력 그리고 환원력과 총페놀 함량과의 상관관계는 각각 r=0.9861와 r=0.9889로 고도의 상관관계를 나타내었다. 이상의 결과를 종합하여 보면, 블랙 라스베리를 수확함에 있어 완숙과보다는 미숙과가 약리적인 측면에서는 유용한 성숙단계라 할 수 있지만 크기나 무게 그리고 맛이나 과색에서는 완숙단계가 식용으로는 적합하고 항산화 활성 등에서는 낮으나 이용 가능한 약리 효과를 가지고 있다고 할 수 있다.

창포의 뿌리를 주근(main root)과 측근(lateral root)으로 분리하여 3 열풍건조분말의 주된 포화지방산으로는 caprylic acid, pentadecanoic acid, stearic acid 및 heneicosanoic acid 등 10종이 확인되었고, 이들은 각각 주근과 측근의 경우 caprylic acid가 28.35%, 31.44%이었고, 불포화지방산으로는 linoleic acid, palmitoleic acid 및 lin

A supercritical fluid extraction was performed for the extraction of phenolics from grape seeds which up to now have been discarded. The optimum condition for extraction process was predicted through response surface methodology using central composit experimental design. The extraction amount of grape seed phenolics was increased by increasing extraction temperature, pressure, and concentration of co-solvent (ethanol). The optimum extraction conditions were 84.83C, 51.50MPa and 1.27% ethanol. The yield of phenolics using SFE was higher with 3 folds than ethanol and 4 folds than hexane but less than 80% methanol. In the respects of food poisoning, the approved solvents were restricted to ethanol and hexane. So, SFE for extraction of phenolics could be powerful alternative method for solvent extraction.