대두 단백 필름 포장 방법이 쇠고기의 저장 중 품질에 미치는 영향을 측정하기 위하여 저장 중 중량 감소율, pH, TBARS, 미생물 수 (총균수) 등을 측정하였다. 쇠고기는 로즈마리 추출물을 첨가한 대두 단백 필름으로 포장 한 후 에 저장하였다. 중량 감소율은 대조구에 비해 포장재를 사용한 것이 현저하게 감소했으며 대두 단백 필름 포장(NPF)과 로즈마리 추출물을 첨가한 대두 단백 필름 포장(RPF)간의 차이는 보이지 않았다. 저장 중 pH의

This study was carried out to know the variation of soybean seed proteins, 11S and 7S globulins, and their amino acid compositions among different colored soybean varieties, 'Danbaegkong' (yellow), 'Pureunkong' (green) 'Jinyulkong' (brown), and 'Geoumjeongkong l' (black). Soybean seed proteins showed a wide range in molecular size, but the electrophoresis patterns of total seed protein subunits showed a similarity among different colored soybean varieties. Amino acid compositions of total seed proteins were similar for all soybean varieties tested. However, soybean varieties showed low composition rates in sulfur containing amino acids. The composition rates of cysteine and methionine in the 11S globulins were higher than those of total seed proteins and 7S globulins. Glutamic acid and glycine were higher in the 11S and 7S globulins than those of total seed proteins. However, the levels of methionine and phenylalanine are high in the 11S globulins, but those of valine and lysin are slightly lower than the 7S globulins. By using HPLC, we tried to analyse the soybean seed proteins. The 11S globulin was composed of 10 major peaks whereas the 7S globulin was composed of 4 major peaks. The composition rates of 11S related proteins have a tendency to increasing during the maturing whereas those of 7S related proteins have a tendency to decreasing. Composition rates of each peaks among different colored soybean varieties suggested that soybean seed proteins are varied, although they showed similarity in the electrophoresis patterns, and understanding of this characteristics is important for the utilization of soybeans.

To improve the iron content of red pepper, we have transferred the entire coding sequence of the ferritin gene(Fpl) into Capsicum annuum (L. cv. Chungyang and Bukang) by Agrobacterium mediated transformation. Transformants were found to contain the Fp1 gene at up to three loci, increased distinct iron content changes. In transgenic plants, iron content was as much as 7-fold to 8-folds greater than that of their untransformed counterparts. Furthermore, the Rl progenies from transformant(A7, A8) co-segregated into a 15:1 ratio for both Kanamycin resistance and genotype of high iron.

Soybean is an important crop because its seed has very high protein relative to others. The quality of soy protein is limited by the concentration of the sulfur-containing amino acids in the amino acid profile. Among the supply of various forms of 0.4mM sulfur as S nutrition during seed fill. only 0.4mM L-methionine can inhibit β-subunit synthesis completely and produce the highest glycinin-containing seeds. Compared to 0.4mM sulfate control, seeds supplied by 0.4mM L-methionine have lower α-, no β-subunit, and highly increased glycinin without altering total protein concentration. Supply of 0.2mM cystine (0.4mM S) did not affect the accumulative pattern of seed storage protein (SSP) subunits. In the supply of L-methionine, 0.2mM treatment showed higher glycinin in seeds but 0.05mM resulted in lower glycinin than tile sulfate control. The relative abundance of α`-subunit was not altered by any N or S nutrition. Under 5mM nitrogen, protein concentration was increased about 3-5% by substituting ammonia for nitrate during seed fill independent of nutrition. The increase resulted in the only increase of 7S protein, mainly β-subunit. Our data suggest that the regulatory system of SSP genes responds to the balance between N and S assimilates supplied from mother plant. and controls the di fferential synthesis of their subunits for the maximum protein accumulation in developing soybean seed.

A cDNA Fragment encoding iron storage protrin generated by polymerase chain reaction(PCR) using highly conserved regions of ferritin related genes were used to sereen a red pepper cDNA library. cDNA clone was designated as Fp1. Fp1 clone contatines a 5' nontranslated region of 51dp containing stop conds. Down stream from 5' UTP. an open reading frame of 750bp was observed. followed by a 3' UTR of 272bp. The deduces amino acid sequence of red pepper protein(Fp1) showed 84%, 48% and 36% identity with soybean(SolC). human(HuL H) and horse spleen(HoS-L) ferritin mRNA accumulation in response to iron. Ferritin mRNA accumulation was transient and particularly abundant in leaves. reaching a maxmum at 12h. The level of ferritin mRNA in roots was affected to a lesser extent than in leaves.

땅콩 품종에 있어서 종자발아 동안에 단백질의 함량 및 pattern(subunits)의 변화를 조사하였던 바 다음과 같은 결과를 얻었다. 땅콩의 저장단백질 패턴이 다른 남대와 대광의 자엽부에 대한 함수율의 변화는 거의 비슷하였으나 배축부에서는 치상 2일과 3일에 남대가 75.0％, 89.1%였고, 대광이 66.0%, 83.5%로서 남대가 다소 높은 함량을 나타내었다. 가용성 단백질의 함량 변화는 남대와 대광 두 품종 공히 자엽부에서는 2∼3일, 배축부에서는 치상 1∼2일 사이에 현저히 감소되었으며, 치상 5일에 자엽부에서는 28∼29%, 배축부에서는 10%의 함량을 나타내었다. 남대와 대광 두 품종의 arachin의 함량은 자엽부, 배축부에서 모두 남대 품종이 높은 값을 나타내었고, 발아 동안에 생성된 new protein은 자엽부에서 3일, 배축부에서 2일에 최고량을 보였다. arachin type이 상이한 남대와 대광 품종에서 발아동안에 대광의 자엽부에서만 치상 2일경부터 16kD의 band를 나타내었다. 발아가 진행됨에 따라 남대와 대광 품종의 자엽부에서는 66, 43, 40, (35.5)kD의 subunit들이 현저히 감소되어 치상 4일경에 소실되었고, 21.5∼23.0kD의 subunits는 완만한 감소로 유지되었으며, 14∼16kD의 low polypeptides는 점차 승가를 나타내었다. 또한, 배축부에서도 치상 2일 동안에 자엽부와 같은 경향을 나타내었다.