The effects of the transferred green rice leafhopper (GRH) resistant gene on yield and grain quality were analyzed by using 7 near-isogenic lines (NILs) with introgressed GRH resistance gene in rice cultivar "Ilpumbyeo". The genotype analysis using SSR markers indicated that all NILs were recovered more than 95% as compared with recurrent parent "Ilpumbyeo". All NILs showed no significant differences compared to recurrent parent for grain yield. No significant differences were observed between NILs and their recurrent parent for physicochemical traits (protein content, amylose content and alkali spreading value) and eating quality. But there were significant differences for appearance of brown and milled rice and pasting properties between some NILs and their recurrent parent. It means that these grain appearance and pasting traits of NILs were very similar or more improved compared to recurrent parent "Ilpumbyeo". Thus, it could be possible to develop GRH resistant variety with high ,quality in segregating populations.

ABSTRACT: Wide hybridization was used to broaden the gene pool of japonica rice. Different approaches involving direct crosses between four japonica cultivars and eight wild species in combination with anther culture, embryo rescue, and molecular markers were used to produce interspecific hybrids, advanced alien introgression lines, dihaploids, and characterization of alien introgression. Interspecific hybrids were produced between Jinmibyeo, Ilpumbyeo, and Hwaseongbyeo cultivars of rice (O. sativa, 2n=24 AA) and wild species, O. rufipogon (2n=24, AA), O. longistaminata (2n=24, AA), O. punctata (2n=24, BB), O. minuta (2n=48, BBCC), O. alta (2n=24, CCDD), including African rice species, O. glaberrima (2n=24, AA). Crosses involving species other than A genome were produced through embryo rescue and were sterile. Following backcrossing with the recurrent japonica parents, advanced progenies have been produced for transfer of alien genes into japonica rice. As results of yield trials, three elite lines were generated from this cross; they are "Suweon 487" with resistance to black streak dwarf virus, "Suweon 497" with blast and bacterial blight resistance and "Suweon 506" with blast resistance, emonstrating that wild species genes have now become important component of japonica rice breeding.

화성벼와 O. minuta 간 여교잡을 통해 반복친인 화성벼와 출수기, 간장 등 여러 작물학적 특성에서 뚜렷한 차이를 보이는 염색체단편 치환계통 "WH79006"을 육성하였는데 화성벼와 WH79006의 차이는 WH79006에 이입된 O. minuta 염색체 단편에 의한 것이라고 할 수 있다. WH79006이 화성벼의 유전적 배경에 O. minuta의 어느 염색체 단편을 가지고 있는지를 검정하기 위해 SSR마커를 이용하였다. 벼 염색체에 골고루 분포한 294개의 SSR 마커를 검정한 결과 최소한 28개의 이입 단편을 검정하였는데 이들은 2번 염색체를 제외한 모든 염색체에 분포하였으며 전체 길이는 약 168cm이었다. 간장 관여 QTL을 탐색하기 위해 화성벼/WH79006 조합의 75개 F2 개체를 육성, 간장을 조사하였다. QTL분석 결과 6번 염색체의 RM225부근에서 간장에 관여하는 QTL cl6가 탐지되었다. cl6는 전체 표현형변이의 9.6~% 를 설명하였으며 O. minute의 대립유전자가 간장을 크게 하는 방향으로 작용하였다. 이 결과는 O. minuta가 포복형임에도 불구하고 간장 조절 유전자들 중에는 간장을 크게 하는 방향으로 작용하는 유전자가 있음을 제시하고 있으며, 이 유전자들은 앞으로 QTL 분석을 통해 그 작용을 밝힐 예정이다. 추후교잡 집단을 이용 O. minuta 특이적 단편들이 어느 형질과 관련이 있는지를 밝힐 예정이다.

Rye genome- and chromosome-specific DNA markers were selected to easily identify the existence of rye chromatin in the wheat genome by RAPD analysis. Among 260 decamer primers used, five primers, namely, OPC01, OPF07, OPF11, OPH09, and OPH16 amplified rye