This study was to evaluate the protein profile of seminal plasma using 2-DE in Hanwoo. Seminal plasma was harvested from five mature Hanwoo, and seminal plasma protein was extracted by M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was . Immobilized pH gradient (IPG) strip was used 18 cm and 3~11 NL. SDS-PAGE was used 12% acrylamide gel. Each gels were visualized by comassie brilliant blue and silver staining. These spots were analyzed by MALDI-TOF MS and searched on NCBInr. The result, 20 proteins of 36 protein spots were searched through peptide sequencing on the NCBInr. 8 proteins profiled by 2-DE were proved through previous bovine studies and the name of each protein was albumin, nucleobindin, clusterin, TIMP-2, spermadhesin Z13, spermadhesin-1 and BSP proteins (BSP 30 kDa and BSP A1/A2). 12 new proteins were ATP synthase, protein MAK16 homolog, Transmembrane protein 214, E3 ubiquitin-protein ligase BRE1A, dual serine/threonine and tyrosine protein kinase, tissue factor pathway inhibitor 2, alpha-actinin-4, RUN domain-containing protein 3B, catenin alpha-1, protein-glutamine gamma-glutamyltransferase 2, plakophilin-1 and inter-alpha-trypsin inhibitor heavy chain H1 has not been previously described in the bovine seminal plasma study. These proteins may be contribute to define the type of proteins affecting fertility of male and improve the fertilizing ability of semen in Hanwoo.

In this paper, a best-first branch and bound algorithm based upon the bottom-up approach for the unweighted unconstrained two-dimensional cutting problem is proposed to find the optimal solution to the problem. The algorithm uses simple and effective meth

The early diagnosis of bovine pregnancy is an essential component of successful reproductive planning on farms, because lack of bovine pregnancy over the long term results in reproductive failure and low milk yield‐the latter of which is a special concern on dairy farms. This study was designed to identify early pregnancy‐specific whey proteins in bovine, by comparing milk samples collected from cattle during pregnancy (Days 30 and 50) and from non‐pregnant cattle. In this study, differentially expressed proteins in five pregnant and five non‐pregnant Holstein dairy cattle were investigated and compared, using proteomics analysis. The first dimension was applied to a pH 3.0～10.0 strip, by loading a 2‐mg milk protein sample. After the second‐dimension separation was performed, the gels were stained with colloidal Coomassie brilliant blue. The stained gels were scanned and the images were analyzed, to detect variations in protein spots between non‐pregnant and pregnant cattle milk protein spots, using ImageMaster; this was followed by analysis with MALDI TOF‐MS. Analysis of the 2‐DE gel image resulted in a total of approximately 500～600 protein spots, of which 12 spots were differentially expressed, six spots were up‐regulated, and four spots were downregulated; two spots were identified as pregnancy‐specific proteins. These proteins were identified as lactoferrin, NADH dehydrogenase subunit 2, albumin, serum albumin precursor and transferrin. Our results via 2‐D PAGE analysis revealed composite profiles of several milk proteins related to early bovine pregnancy, implying the possible use of these milk proteins in the early detection of bovine pregnancy.

본 논문은 로터 시스템의 디스크 회전운동을 표현하는데 있어 운동방정식을 통합하는 과정에서 기존 연구자들이 채택한 오일러 각 사용법이 일관성이 없음을 지적하였다. 기존 연구자들은 오일러 각 순서가 달라서 속도와 운동에너지도 달리 산정하였음은 물론, 운동방정식은 오직 선형 시스템만 취급해 왔다 이러한 오일러 각 사용법의 단점을 극복하기 위하여 회전운동을 더욱 단순하게 매개화할 수 있는 4원법(quaternion)과 구 좌표계를 적용하여 비선형 시스템을 도출하였다. 이를 바탕으로 수치해석을 통하여 기존 방법과 비교하여 제안한 방법의 신뢰성과 우수성을 보였다.

SnO nanosheets were prepared at room temperature through a reaction between an aqueous solution of SnCl2, N2F4, and NaOH and were converted into SnO2 nanosheets without a morphological change. The SnO nanosheets were formed through a dissolution-recrystallization mechanism. Uniform and well-dispersed SnO nanosheets with the round-shape morphology were attained when the solution was treated by ultrasonic sound immediately after the addition of NaOH. The SnO2 nanosheets prepared by means of solution reduction under the ultrasonic treatment, and subsequent oxidation at 600˚C showed a high level of gas sensitivity to C2H5OH and CH3COCH3.

본 연구는 2차원 및 3차원 동적 탄소성 응력 해석을 위한 특수 적분해 경계요소법의 공식 개발을 제시한다 정적 탄성에 대한 기본식이 일반해를 구하는데 이용되었으며, 전체형상함수 개념을 이용하여, 변위율과 traction rate의 특수 적분해를 구함으로써 지배 방정식의 가속도 부분을 근사화시켰다. 시간 적분을 위하여 Houbolt 시적분 방법을 이용하였으며, Newton-Raphson 알고리즘을 이용하여 수치 연산을 행하였다. 제시된 공식에 따른 예제 해석을 통하여 그 방법의 유효성과 정확성을 설명하였다.

The Fokker-Planck (FP) model is one of the commonly used methods for studies of the dynamical evolution of dense spherical stellar systems such as globular clusters and galactic nuclei. The FP model is numerically stable in most cases, but we find that it encounters numerical difficulties rather often when the effects of tidal shocks are included in two-dimensional (energy and angular momentum space) version of the FP model or when the initial condition is extreme (e.g., a very large cluster mass and a small cluster radius). To avoid such a problem, we have developed a new integration scheme for a two-dimensional FP equation by adopting an Alternating Direction Implicit (ADI) method given in the Douglas-Rachford split form. We find that our ADI method reduces the computing time by a factor of ~2 compared to the fully implicit method, and resolves problems of numerical instability.

Many contemporary architectural avant gardes tend to use painting as a medium to create architecture which goes beyond the rationalized spatial conception of modem architecture represented by perspectivism. They produce non perspective drawings to represent spatial Ideas, and expand it through poetic imagination to create an unexpected architectural form and space. This paper attempts to analyze the historical origin and background of dominance of drawing in the production of architecture. It was with the invention of perspective that architectural representation became important tool for architectural production. Thereafter, drawing was considered prior to actual building and architecture was considered a three dimensional realization of two dimensional drawing. Modernist avant gardes such as Cubism shattered the rationalized pictorial space of perspective and found a new pictorial space. They tried to extend it to three dimensional space through parallel projection largely based on the Hildebrand's theory of pure visibility. However, due to the ambiguity of the position of the viewing subject, their attempts could not succeed in creating a new architecture. The new architectural avant garde of the 70's rediscovered the early 20th century avant gardes in their attempt to create a new architecture which can register the fragmented spatial condition of contemporary society, and used painting as a medium to create architecture. Their difference from the early avant gardes was that they used poetic imagination rather than parallel projection in the process of projecting three dimensional space and form from the painting. However, their architecture cannot escape the scopic field of perspectivism in that they rely on the picture plane and the distance between object and viewing subject. Therefore, I conclude that in order to create architecture which goes beyond the rationalized space of modern architecture, it is necessary to resort to other tradition of modern architecture than visual one.

이 논문에서는 포화된 2상 지반의 동적해석에서 원역을 모형화하기 위한 새로운 무한요소를 제안하였다. 무한요소법은 무한영역 또는 반무한영역을 모형화해야 하는 공학문제에 효과적으로 적용되어 왔다. 그러나 현재까지 개발된 2상지반의 동적해석을 위한 무한요소는 형상함수에 사용될 수 있는 파동성문이 2개(Pl파와 P2파)로 한정되어 있다. 이 논문에서는 이와 같은 제한을 없애고 임의 개수의 파동성분을 고려할 수 있도록 하는 정식화 과정을 제안하였다. 구조물을 포함하는 근역은 유한요소로 나타내며 원역은 평행층상 반무한 지반으로 가정하였다. 제안된 무한요소의 타당성은 1차원 및 2차원 파동전달문제를 해석하고 이를 이론해 및 정밀수지해석 해와 비교하여 검증하였다.

To investigate the antifungal activity related protein in pesticidal bacteria, a bacterial strain LTD was isolated from soil collected at Gimje in Jeonbuk province, Korea, and identified as Bacillus subtilis LTD based on a API50 CHB kit and 168 rDNA seque

Here we present a linear stability analysis and an MHD 2D model for the Parker-Jeans instability in the Galactic gaseous disk. The magnetic field is assumed parallel to a Galactic spiral arm, and the gaseous disk is modelled as a multi-component, magnetized, and isothermal gas layer. The model employs the observed vertical stratifications for the gas density and the gravitational acceleration in the Solar neighborhood, and the self-gravity of the gas is also included. By solving Poisson's equation for the gas density stratification, we determine the vertical acceleration due to self-gravity as a function of z. Subtracting it from the observed gravitational acceleration, we separate the total acceleration into self and external gravities. The linear stability analysis provides the corresponding dispersion relations. The time and length scales of the fastest growing mode of the Parker-Jeans instability are about 40 Myr and 3.3 kpc, respectively. In order to confirm the linear stability analysis, we have performed two-dimensional MHD simulations. These show that the Parker-Jeans instability under the self and external gravities evolves into a quasi-equilibrium state, creating condensations on the northern and southern sides of the plane, in an alternate manner.

본 연구에서는 경계 요소법을 이용하여 2차원 비등방성 탄성체의 손상 규명을 수행한다. 경계에서의 적분항만을 포함하는 본 수치모델은 1차원으로 줄게된다. 이러한 장점은 특히 균열 역학과 같은 문제에 있어서 중요한 의미를 갖는다. 또한 일정 영역을 분할하는 기법을 피함으로서 수치해석 과정을 간편하고 효율적으로 수행할수 있기 때문에 역문제 해결에 있어서 장점을 갖는다. 본 연구에서는 기존의 등방성 재료에 대한 비파괴 추정기법을 복합신소재와 같은 비등방성 재료로 이루어진 탄성체의 해석에 대하여 확장한다. 먼저 경계요소법에 의한 수치모델의 타당성을 기존의 문헌과 비교 검증하며, 서로 다른 특성을 보이는 비등방성 형식의 변화에 따라 실제 측정시 발생하는 노이즈 영향을 분석한다. 수치예제는 적층 형태 및 하중조건에 대하여 수행하며, 결함 추정에 미치는 적층 형태의 영향을 시험한다.

본 연구는 국제정서사진체계(IAPS) 사진 중 이차원 감성을 잘 표현한다고 판단되는 9장의 사진을 선정하고, 이 사진 자극으로 유발된 감성의 변화를 심리ㆍ생리적으로 측정한 후 이차원 감성의 한 축인 각성/이완의 감성변화를 심리ㆍ생리적으로 변별 가능한지를 검증하는 것을 목표로 한다. 각성/이완의 감성변화를 대변하는 Eelectrocardiogram (ECG), Galvanic Skin Response (GSR), Skin Temperature, Respiration 등의 자율신경계 반응을 측정하였다. 자극 제시 후에 주관적 평가를 실시한 바, 각성 〉 불쾌/각성 및 쾌/각성 〉 중립 〉 이완 순서의 4단계로 각성도 감성을 구분할 수 있었다 자율신경계 반응은 각성, 불쾌/각성, 쾌/각성 〉 중립 〉 이완 순서의 3단계로 구분이 가능하였다. 본 연구는 한국인의 이차원 감성을 표현할 수 있는 9장의 사진을 IAPS로부터 선정하였으며, 이차원 감성의 한 축인 각성/이완의 감성 변화를 심리ㆍ생리적평가로부터 변별할 수 있다는 사실을 확인하였다.

본 논문에서는 2차원 사각탱크내 비압축성, 비점성, 비회전 유동에 대한 비선형 슬로실 해석을 다룬다. 유체영역의 지배방정식으로 포텐셜 이론에 기반을 둔 라플라스 방정식을 사용한다. 대변형의 슬로싱 거동을 표현하기 위하여 베르누이 방정식으로부터 유도된 운동 및 동역학적 자유표면 경계조건을 적용한다. 이러한 비선형 슬로싱 문제는 9결점 요소를 사용한 유한요소법에 의하여 해석되어 진다. 경계조건에 대한 시간적분과 정확한 속도계산을 위하여 각각 예측자-수정자 기법 및 최소자승법을 도입하였다. 또한, 자유표면 추적에서 야기되는 안정성 문제는 시간변동에 대한 자유표면 위치를 직접 계산함으로써 확보할 수 있었다. 외부 조화가진에 대한 본 논문의 결과는 선형이론해 또는 참고문헌의 결과와 비교하여 매우 정확하고 안정적이었다. 프로그램 검증 후, 유체높이와 가진크기에 대한 슬로싱 응답특성을 분석하였다.