We examined the effects of cadmium exposure and various temperature stress on the expression of Pardosa astrigera heat shock protein 70 (HSP70). To do this, P. astrigera HSP70 gene was cloned and its sequence determined. Female spiders collected from non-contaminated region were exposed to 40mM CdCl2 for 2, 4 and 6 weeks by dietary uptake. At the end of every 2, 4 and 6 weeks of exposure, a batch of 5 spiders was collected and total RNA was extracted from each batch of whole bodies. Female spiders were also exposed to different temperatures (20, 25, 30 and 35℃) for 3h and RNA extracted likewise. Transcription profiles of HSP70 in response to cadmium and temperature were determined by quantitative real-time PCR using 18S rRNA as reference gene for data normalization. HSP70 transcription gradually increased during 2,4 and 6 weeks of exposure to cadmium. In particular, the expression level at 6-week exposure was 3.4-fold higher than that of untreated control. In the temperature response, an increased expression of HSP70 was also observed as temperature increased up to 30℃ and then slightly decreased at 35℃. The expression level at 30℃ was 2.3-fold higher than that of 25℃. Taken together, HSP70 gene appears to be up-regulated by general stress factors including cadmium exposure and temperature increase.
A Korean Cheiracanthium erraticum (Walckenaer, 1802) is first described with detailed illustrations, leg spination, trichobothrium patterns and SEM photographs of male palp and female epigynum. Two species, C. lascivum Karsch, 1879 and C. eutittha Boesenb
호주산 독거미의 일종인 Red-Back Spider (Latrodectus hasselti)는 고온의 사막이나 저온의 산악지역을 제외하고 호주 전 지역에서 발견되는 거미로 뉴질랜드의 Katipo Spider나 미국의 Black Widow Spider와 같은 과에 속하는 것으로 알려져 있다. 호주에는 1속 1종이 보고된 바 있으며, 독거미 중에서는 가장 잘 알려져 있는 종이다. 이에 저자는 호주 채집여행을 하며 채집한 종들 중 Red-Back spider
A list of Korean spiders was revised, including 46 families 225 genera 622 species which were reported by December 1999. Of these 61 added species and 37 alterated scientific names were supplemented, and 3 species, which have been reported since June 1994, were newly synonymized. The spider fauna of Korea is composed of 12 Cosmopolitan, 34 Holarctic, 66 Palearctic and 136 Korean endemic species. The spider fauna of Korea has much more similarity with Japanese spider fauna than with those of other countries.
초 록
최근 중국에서 신종기재된 Neoscona속(거미목: 왕거미과)의 2종 Neoscona minoriscylla(아기지이어리왕거미)와 Neoscona pseudonautica(어리집왕거미)를 한국미기록종으로 보고한다. 이로써 한국산 Neoscona속은 기지종 8종과 함께 10종이 된다.