Background : Codonopsis is a flowering plants belong to the family Campanulaceae, and has many kinds of medicinal properties. As currently recognized, two other groups, Campanumoea and Leptocodon, are included in the Codonopsis. The enlarged genus Codonopsis is distributed in Eastern, Southern, Central, and Southeastern Asia. C. lanceolata, C. clematidea and C. pilosula has many kinds of medicinal properties and this plants are used as medicinal and edible plants. C. ovata and C. mollis are distributed in Pakistan Kashmir and Himalaya mountains at an altitude of about 3,000 m, and flowers bloom in July to August. Methods and Results : In this study, we analyzed the genetic diversity of 5 Codonopsis species using 8 SSR markers base on C. lancelolata genomic sequences. Samples were obtained from fresh leaves of 5 plants from each species and genomic DNA was extracted using CTAB method. PCR was performed in total 20μl reaction volume containing 20 ng of DNA template and 5 pmole of primers. PCR conditions composed pre-denaturation at 95℃ for 5 min, then 35 cycles of 95°C for 30 sec, 60°C for 30 sec and 72°C for 30 sec, and a final extension at 72℃ for 30 min. The amplified band sizes ranged from 74 to 301 bp and clearly showed single or doble bands in eletrophoresis. From the phylogenetic analysis, C. lanceolata was grouped together, but the others were not grouped together according to the species. Conclusion : We concluded that C. lanceolata cultivated in Korea is different from the other species, and the eight SSR markers used in this study are able to distinguish C. lanceolata from the other species.

Formation of disinfection by-products (DBPs) including trihalomethans (THMs) and haloacetic acids (HAAs) from chlorination of six different species (Chlorella vulgaris, Scenedesmus sp., Anabaena cylindrical, Microcystis aeruginosa, Asterionella formosa and Aulacoseira sp.) of algal extracellular organic matter (EOM). The EOM characteristics evaluation of six algal species reaching at the stationary phase in the growth curve showed most of its SUVA254 showed below 1 and this means hydrophilic organic matter is much higher than hydrophobic organic matter. Chloroform formation potential (CFFP), dichloroacetic acid formation potential (DCAAFP) and trichloroacetic acid formation potential (TCAAFP) were mainly composed of THMFP and HAAFP in the EOM of various algal species. In the case of THMFP/DOC and HAAFP/DOC values, EOM of blue-green algae has appeared highest and EOM of green algae and diatom in order. THMFP/DOC was higher than HAAFP/DOC in EOM of blue-green algae. In comparison of formation potential by unit DOC composed of HAAFP in algal species EOM, DCAAFP/DOC was 1.5 times to 7.5 time higher than TCAAFP/DOC in the EOM of blue-green algae, while DCAAFP/DOC was found to be relatively high compared to TCAAFP/DOC in the EOM of green algae and diatom.

This study aims at classifying and interpreting on the vegetation structure, the correlation between a vegetation and an environment, a species diversity and a life-form of Evergreen Broad-Leaved Forest(EBLF) located in Gageo-do Island. It is also the objective that the estimation of vegetation change founded on the species composition and characteristics. The vegetation of EBLF was classified into three forests or four community units as Machilus thunbergii forest (Polystichum polyblepharon-M. thunbergii community and Phaenosperma globosum-M. thunbergii community), Ilex integra-Castanopsis sieboldii community, Quercus acuta community and Neolitsea sericea stand. The ordination analysis by DCA is analogous with the vegetation structure analysis. As a result of the correlation (Pearson's correlation coefficient) with environmental conditions, the Altitude has the significance with the distribution of communities. The total vegetation change by progress of succession will not be wandered away from the present vegetation structure practically, and the vegetation on the underlayers will be a little changed.

A biological assessments of fish community structure were carried out in the lower reach of Seomjin River from May 2009 to November 2010. The collected fish from the six surveyed sites were 63 species belonging to 24 families, and 10 orders. Although species was different depending on sites, the numbers of individuals were not shown significance on sites. Locational dominant species were different. For example, the dominant species on May were Zacco platypus at W-1, Zacco temminckii at W-2, Acanthogobius flavimanus at W-3 and W-4, and Leiognathus nuchalis at W-5 and W-6. There is no seasonal differences in species. Species diversity was the best up to 2.64 on May at W-1 from 1.33 on November at W-6. As a result of an analysis about environmental factors for the numbers of fish species and individuals in each surveyed sites, the most effective groups were DO, BOD, and COD. The proportional difference was high on May than November between transient and asymptotic projections for population size and population growth rate for simulations starting at the current or theoretical stage distribution.

This research was carried out to investigate invertebrate fauna with organic and conventional pear orchards, which used four collected methods; soil sampling for soil microorganism, pitfall, malaise, and black light trap for over ground species. Collected species were 37 species, 1,184 individuals in organic and 28 species, 501 individuals by soil sampling in conventional pear fields. Those were 38 species, 646 individuals and 29 species, 440 individuals by pitfall trap, 55 species 650 individuals and 47 species, 508 individuals by malaise trap, and 23 species, 201 individuals and 9 species, 42 individuals by black light trap. Collembola was collected 389 individuals in organic which was 5 times than in conventional in soil sampling. In pitfall trap, that was 183 individuals which was 3 times. The diversity indices of organic pear orchards were 1.956 in May, 2.638 in August and those of conventional was 1.426 in May, 2.011 in August in soil sampling. In pitfall trap, the dominant species were spiders, collembollan, and coleopteran. Among Coleoptera, indicator insects for the evaluation of agricultural environment suggested were Eusilpha jakowelewi as organic pear orchard and Anisodactykus punctatipennis and Pheropsophus jessoensis as conventional. Malaise trap was collected dominant species as Diptera and Hymenoptera of Braconidae and Ichneumonidae. The diversity indices of organic pear orchards were 2.952, 3.120, and 2.010 in pitfall, malaise and black light trap in over ground invertebrate sampling. The highest diversity was in malaise trap. The higher diversity indices, the lower dominance indices.

Shannon and Simpson indexes are used for species diversity analysis of ecosystem. In species diversity analysis of ecosystem, not only frequency of each species but also survey size have to be considered. In this study, total information composed with knowledge and ignorance was suggested as a species diversity analysis method for ecosystem survey. To apply developed method, flora in the Sangachun river valley was sampled with 19 sites and 198 species. In applying results, Shannon index shows more reasonable results than Simpson index by the variance of sample size but has difficulties of determining the relation of surveying species number and sample site number. Suggested total information can overcome this difficulty by the relation of knowledge and ignorance.

We examined the genetic variation within the species, the patterns of genetic diversty between populations, thermostability variations of enzymes and temperature tolerances of Corbicula japonica from the two main rivers in Korea. Starch gel electrophoresis was used to examine the genetic variation of 22 loci. Heating experiments of electrophoresis under the condition of 40±5° for 15±5 min disclosed thermostability differences, called heat-sensitive and heat-resistant types, within each electrophoretic allozyme. Genetic diversity at the natural species level was high (77.3%), whereas the extent of heat-treat groups was relatively low (52.6%). The genetic diversity trends to decrease from the source of two main rivers (the Sumjin River and the Nam River) to the mouths. Based on the data available such as considerable high genetic diversity compared with a mean value of C. japonica species, It is recommended that several populations of the species in Korea should be preserved.